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The map label for this gene is pbpE [H]

Identifier: 116621660

GI number: 116621660

Start: 3219014

End: 3220198

Strand: Reverse

Name: pbpE [H]

Synonym: Acid_2542

Alternate gene names: 116621660

Gene position: 3220198-3219014 (Counterclockwise)

Preceding gene: 116621663

Following gene: 116621659

Centisome position: 32.31

GC content: 63.63

Gene sequence:

>1185_bases
ATGCTCCGCGCCATTGGCTGGTGCCGCACCAAAGCGATTATCCTGAAATCAGTGACACCCCGCTTCCTCATACCCATAGC
GCTTTCGCTCGCCTCCGCCGTTGCGTTCGCCGCGGACCCGCCGCTCGATACCATCTTTGCTCCCCTGGCAGGTCCCGATA
CTCCCGGGCTTGCCGTTCTCGTGCGCCAGGATGGACGCACCGTATTCCAGCACGGCTATGGCGTGCGTGATTTGCGCTCG
CTCGCCAAAATTGATACCGCCACCGATTTCCGCCTGGCCTCCTTCACCAAGCAGTTCACCGCCATGGCCATCATGCTGCT
CGTCCACGATGGCAAGCTGCGCTACGACCAGACACTCACTGAGATCTTTCCGGACTTCCCCGCCTACGGAAAGACGATCA
CCATCCGCCACCTGCTTACCCACACCGCCGGGCTCCCCGATTACGAAGACCTGATGGATGAGGCCGAAAAAGGCAAGCCG
CCCATCTGGACGCTCGCGCATCAGATTCAGGACCGCGAAGTGCTCGACCTGCTCAAGCAGCAGACCCGGCCGAAGTTCGC
CGCGGGCACGAGCTGGTCCTACAGCAATTCCGCCTATGTGGTGCTCGGCCTGATCGTCGCCAAGGTCTCCGGTCAGACCT
ACGGGGACTTTCTGCGCGCGCGCATCTTTCAGCCGCTCCACATGGATCAGACGCTGGCCTTCGTCAACGGCTCCAACACC
GTGCCCAATCGCGCCTACGGCCACTCCAAAAGACAGGGCAAGTTCGTGCAGACCGACCAGAGTCCGACTTCCGCGACGCT
CGGCGATGGCGGCGTCTATTCCAACATCGCCGATCTCGCCAAGTGGGACGACGCACTCGCCCGCCACACTCTGCTCGGTG
AACCGGAAATGCAGCCCGCGCTCGTGCCCGCAAAGCTGGCCGACGGCTCCGCGACCCGCTGGCCCAACACGCCCGGCGGC
GACAACCTGGCCCCCGGCCGTCCCGTGGAGTACGGCTTCGGCTGGTTCCTCGACTCCTGGCAGGGCCGTCCGCGCATGTG
GCACACCGGCACTACTCAGGGATTCCGTACCGTGATCGAGCGTTTCCCGGAATCGCAAGTCAGCGTGATCATCCTAAGCA
ACCGCACGGATCTGGACCCCGCCGCGCTCGCCGAGCGAGTCGCGGGCCAGATACTGAAGAAGTGA

Upstream 100 bases:

>100_bases
GGTCGGTTCAAAGCGCAGCAGCCGGTCGAGCGGCTCCTCGATTGAGGTTGAAACTTCTTGTATGGTTTCCGTCTTCGTGG
CCATCGCGACCGTATACAGC

Downstream 100 bases:

>100_bases
CCCATGGCTTCCACACCCTCCAGAACGCCCTTCTCGGTCCTCGATCTGGCGCCCGTGCGCCAGGGCGGAACTCCCGCTGA
AGCCTTCCGCAACTCGCTCG

Product: beta-lactamase

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLRAIGWCRTKAIILKSVTPRFLIPIALSLASAVAFAADPPLDTIFAPLAGPDTPGLAVLVRQDGRTVFQHGYGVRDLRS
LAKIDTATDFRLASFTKQFTAMAIMLLVHDGKLRYDQTLTEIFPDFPAYGKTITIRHLLTHTAGLPDYEDLMDEAEKGKP
PIWTLAHQIQDREVLDLLKQQTRPKFAAGTSWSYSNSAYVVLGLIVAKVSGQTYGDFLRARIFQPLHMDQTLAFVNGSNT
VPNRAYGHSKRQGKFVQTDQSPTSATLGDGGVYSNIADLAKWDDALARHTLLGEPEMQPALVPAKLADGSATRWPNTPGG
DNLAPGRPVEYGFGWFLDSWQGRPRMWHTGTTQGFRTVIERFPESQVSVIILSNRTDLDPAALAERVAGQILKK

Sequences:

>Translated_394_residues
MLRAIGWCRTKAIILKSVTPRFLIPIALSLASAVAFAADPPLDTIFAPLAGPDTPGLAVLVRQDGRTVFQHGYGVRDLRS
LAKIDTATDFRLASFTKQFTAMAIMLLVHDGKLRYDQTLTEIFPDFPAYGKTITIRHLLTHTAGLPDYEDLMDEAEKGKP
PIWTLAHQIQDREVLDLLKQQTRPKFAAGTSWSYSNSAYVVLGLIVAKVSGQTYGDFLRARIFQPLHMDQTLAFVNGSNT
VPNRAYGHSKRQGKFVQTDQSPTSATLGDGGVYSNIADLAKWDDALARHTLLGEPEMQPALVPAKLADGSATRWPNTPGG
DNLAPGRPVEYGFGWFLDSWQGRPRMWHTGTTQGFRTVIERFPESQVSVIILSNRTDLDPAALAERVAGQILKK
>Mature_394_residues
MLRAIGWCRTKAIILKSVTPRFLIPIALSLASAVAFAADPPLDTIFAPLAGPDTPGLAVLVRQDGRTVFQHGYGVRDLRS
LAKIDTATDFRLASFTKQFTAMAIMLLVHDGKLRYDQTLTEIFPDFPAYGKTITIRHLLTHTAGLPDYEDLMDEAEKGKP
PIWTLAHQIQDREVLDLLKQQTRPKFAAGTSWSYSNSAYVVLGLIVAKVSGQTYGDFLRARIFQPLHMDQTLAFVNGSNT
VPNRAYGHSKRQGKFVQTDQSPTSATLGDGGVYSNIADLAKWDDALARHTLLGEPEMQPALVPAKLADGSATRWPNTPGG
DNLAPGRPVEYGFGWFLDSWQGRPRMWHTGTTQGFRTVIERFPESQVSVIILSNRTDLDPAALAERVAGQILKK

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI1790593, Length=403, Percent_Identity=26.0545905707196, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786574, Length=343, Percent_Identity=25.0728862973761, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082101, Length=194, Percent_Identity=27.8350515463918, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17550934, Length=183, Percent_Identity=30.0546448087432, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533026, Length=349, Percent_Identity=25.5014326647564, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 43385; Mature: 43385

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRAIGWCRTKAIILKSVTPRFLIPIALSLASAVAFAADPPLDTIFAPLAGPDTPGLAVL
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEE
VRQDGRTVFQHGYGVRDLRSLAKIDTATDFRLASFTKQFTAMAIMLLVHDGKLRYDQTLT
EECCCCCHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EIFPDFPAYGKTITIRHLLTHTAGLPDYEDLMDEAEKGKPPIWTLAHQIQDREVLDLLKQ
HHCCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHH
QTRPKFAAGTSWSYSNSAYVVLGLIVAKVSGQTYGDFLRARIFQPLHMDQTLAFVNGSNT
HCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCCC
VPNRAYGHSKRQGKFVQTDQSPTSATLGDGGVYSNIADLAKWDDALARHTLLGEPEMQPA
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LVPAKLADGSATRWPNTPGGDNLAPGRPVEYGFGWFLDSWQGRPRMWHTGTTQGFRTVIE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCHHHHHHHH
RFPESQVSVIILSNRTDLDPAALAERVAGQILKK
HCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLRAIGWCRTKAIILKSVTPRFLIPIALSLASAVAFAADPPLDTIFAPLAGPDTPGLAVL
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEE
VRQDGRTVFQHGYGVRDLRSLAKIDTATDFRLASFTKQFTAMAIMLLVHDGKLRYDQTLT
EECCCCCHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EIFPDFPAYGKTITIRHLLTHTAGLPDYEDLMDEAEKGKPPIWTLAHQIQDREVLDLLKQ
HHCCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHH
QTRPKFAAGTSWSYSNSAYVVLGLIVAKVSGQTYGDFLRARIFQPLHMDQTLAFVNGSNT
HCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCCC
VPNRAYGHSKRQGKFVQTDQSPTSATLGDGGVYSNIADLAKWDDALARHTLLGEPEMQPA
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LVPAKLADGSATRWPNTPGGDNLAPGRPVEYGFGWFLDSWQGRPRMWHTGTTQGFRTVIE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCHHHHHHHH
RFPESQVSVIILSNRTDLDPAALAERVAGQILKK
HCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]