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The map label for this gene is pucG [H]

Identifier: 116621633

GI number: 116621633

Start: 3188332

End: 3189456

Strand: Direct

Name: pucG [H]

Synonym: Acid_2515

Alternate gene names: 116621633

Gene position: 3188332-3189456 (Clockwise)

Preceding gene: 116621632

Following gene: 116621635

Centisome position: 31.99

GC content: 64.89

Gene sequence:

>1125_bases
ATGCCGCAAAGCTATCCTGCGATTCAGGCGCCGCCGCGCCTTCTCTTCGGACCCGGACCCAGCATGGTCGCGCCCCGCGT
CTATCAGGCGATGTCACAGCCCGTGGTGGGGCATCTGGATCCATTCTTTTTTCAGGTGGTGGAAGAGGTCCGCGGCCTGC
TCGGGTATGCCTACTCGACGAAGAATCATTTCAACCTCGCGACCTCTGGTACCGGCACCTCGGGGATGGAATGCGCGGTC
GCGAACTTCACCGAGCCCGGCGATAAATTTGCGCTGCTGACCAACGGATTTTTCGGCGATCGCATCGGCGATATGGCGCG
GCGTCATGGCGGCGAGGTGGTGCGGCTCACGAAGCCTTGGGGCGAGCCGTTCGATGCGCAGGAGGTCCGCGAATTCATCC
GCCGGGAACGGCCGCGGGTGGTGGCGTTCGTGCAGGCCGAGACCTCGACCGGGATGTTCAACCAGGCGAAGCCGATCTGC
GACGCGGCGCGCGAAGTGGACGCGGTGACGATCGCCGATTGCGTCACGTCGCTGGGCGGAATGCCGGTGCTGGTGGATGA
GAACGGGATCGACATCGCGTACAGCTGCTCGCAGAAGGGCCTCGGGTGCCCGCCCGGACTGGCGCCGATGACGGTGGGGC
CGCGCGCGCTGGGGCGGCTGAAGGAGCGCAAGGCTCCGGTGCAGTCCTTCTACCTGGACCTGCAGCTGCTCGACAGTTTC
TATTGCGGAAAGAAGTATCACCACACCGCGTCGGCCACGATGTTCTACGCGCTGCGCGAGGGCCTGGCGATTGTCGCGGA
GGAGGGGCTGGAGGCGCGGTGGGCGCGTCACAAGGCAAACCACGAGGCGTTCGTGAAGGGGATCGAGGCGATGGGTTTGA
GCATGCTGGTGCCGGAAGGCCACCGGCTGTGGACGTTGAATACGCCGCGGATTCCCGAGGGCGTGGACGATGCCAAGGTG
CGGCAGTTCCTGCTGGAGCAGTACAACATGGAAATCGCCGGGGGCTTCGGTCCTCTGGCGGGCAAGGTCTTCCGCATCGG
CACCATGGGCTATAGCTCAACGGCGGAGAACGTGCAGCTCATTCTCCAGGCGCTGGAAGAGGCGCTGAAGGCGGCGAAGG
CATAG

Upstream 100 bases:

>100_bases
CAGCGTGGCCGAGCAGCTGGCGGCTTCCTGCGCGCAGATGCAAAGATAACGGCAACGTAGCCGCAACGTCTTTTTCTGCC
GCGGGCTACACTAGTTGCTT

Downstream 100 bases:

>100_bases
AGATCTCCGATCGCCTGCGCGATTCGCTCACCCCATCAGAGCCGCGACCGTCAGGGAGCGTCCTTGCTAACTCAACTAAC
CGCCCAGCCTGGCGGCGAAA

Product: alanine-glyoxylate aminotransferase

Products: pyruvate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MPQSYPAIQAPPRLLFGPGPSMVAPRVYQAMSQPVVGHLDPFFFQVVEEVRGLLGYAYSTKNHFNLATSGTGTSGMECAV
ANFTEPGDKFALLTNGFFGDRIGDMARRHGGEVVRLTKPWGEPFDAQEVREFIRRERPRVVAFVQAETSTGMFNQAKPIC
DAAREVDAVTIADCVTSLGGMPVLVDENGIDIAYSCSQKGLGCPPGLAPMTVGPRALGRLKERKAPVQSFYLDLQLLDSF
YCGKKYHHTASATMFYALREGLAIVAEEGLEARWARHKANHEAFVKGIEAMGLSMLVPEGHRLWTLNTPRIPEGVDDAKV
RQFLLEQYNMEIAGGFGPLAGKVFRIGTMGYSSTAENVQLILQALEEALKAAKA

Sequences:

>Translated_374_residues
MPQSYPAIQAPPRLLFGPGPSMVAPRVYQAMSQPVVGHLDPFFFQVVEEVRGLLGYAYSTKNHFNLATSGTGTSGMECAV
ANFTEPGDKFALLTNGFFGDRIGDMARRHGGEVVRLTKPWGEPFDAQEVREFIRRERPRVVAFVQAETSTGMFNQAKPIC
DAAREVDAVTIADCVTSLGGMPVLVDENGIDIAYSCSQKGLGCPPGLAPMTVGPRALGRLKERKAPVQSFYLDLQLLDSF
YCGKKYHHTASATMFYALREGLAIVAEEGLEARWARHKANHEAFVKGIEAMGLSMLVPEGHRLWTLNTPRIPEGVDDAKV
RQFLLEQYNMEIAGGFGPLAGKVFRIGTMGYSSTAENVQLILQALEEALKAAKA
>Mature_373_residues
PQSYPAIQAPPRLLFGPGPSMVAPRVYQAMSQPVVGHLDPFFFQVVEEVRGLLGYAYSTKNHFNLATSGTGTSGMECAVA
NFTEPGDKFALLTNGFFGDRIGDMARRHGGEVVRLTKPWGEPFDAQEVREFIRRERPRVVAFVQAETSTGMFNQAKPICD
AAREVDAVTIADCVTSLGGMPVLVDENGIDIAYSCSQKGLGCPPGLAPMTVGPRALGRLKERKAPVQSFYLDLQLLDSFY
CGKKYHHTASATMFYALREGLAIVAEEGLEARWARHKANHEAFVKGIEAMGLSMLVPEGHRLWTLNTPRIPEGVDDAKVR
QFLLEQYNMEIAGGFGPLAGKVFRIGTMGYSSTAENVQLILQALEEALKAAKA

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=369, Percent_Identity=35.7723577235772, Blast_Score=247, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17536281, Length=364, Percent_Identity=40.1098901098901, Blast_Score=276, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6321079, Length=370, Percent_Identity=26.7567567567568, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17530823, Length=372, Percent_Identity=39.7849462365591, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.44

Molecular weight: Translated: 40833; Mature: 40702

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSYPAIQAPPRLLFGPGPSMVAPRVYQAMSQPVVGHLDPFFFQVVEEVRGLLGYAYST
CCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCC
KNHFNLATSGTGTSGMECAVANFTEPGDKFALLTNGFFGDRIGDMARRHGGEVVRLTKPW
CCCEEEEECCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCC
GEPFDAQEVREFIRRERPRVVAFVQAETSTGMFNQAKPICDAAREVDAVTIADCVTSLGG
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCC
MPVLVDENGIDIAYSCSQKGLGCPPGLAPMTVGPRALGRLKERKAPVQSFYLDLQLLDSF
EEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
YCGKKYHHTASATMFYALREGLAIVAEEGLEARWARHKANHEAFVKGIEAMGLSMLVPEG
HCCCHHCCHHHHHHHHHHHHCHHEEHHCCHHHHHHHHHCCHHHHHHHHHHCCCEEECCCC
HRLWTLNTPRIPEGVDDAKVRQFLLEQYNMEIAGGFGPLAGKVFRIGTMGYSSTAENVQL
CEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCCCCCHHHHHHH
ILQALEEALKAAKA
HHHHHHHHHHHHCC
>Mature Secondary Structure 
PQSYPAIQAPPRLLFGPGPSMVAPRVYQAMSQPVVGHLDPFFFQVVEEVRGLLGYAYST
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCC
KNHFNLATSGTGTSGMECAVANFTEPGDKFALLTNGFFGDRIGDMARRHGGEVVRLTKPW
CCCEEEEECCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCC
GEPFDAQEVREFIRRERPRVVAFVQAETSTGMFNQAKPICDAAREVDAVTIADCVTSLGG
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCC
MPVLVDENGIDIAYSCSQKGLGCPPGLAPMTVGPRALGRLKERKAPVQSFYLDLQLLDSF
EEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
YCGKKYHHTASATMFYALREGLAIVAEEGLEARWARHKANHEAFVKGIEAMGLSMLVPEG
HCCCHHCCHHHHHHHHHHHHCHHEEHHCCHHHHHHHHHCCHHHHHHHHHHCCCEEECCCC
HRLWTLNTPRIPEGVDDAKVRQFLLEQYNMEIAGGFGPLAGKVFRIGTMGYSSTAENVQL
CEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCCCCCHHHHHHH
ILQALEEALKAAKA
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-alanine; glyoxylate

Specific reaction: L-alanine + glyoxylate = pyruvate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]