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The map label for this gene is pbpG [H]

Identifier: 116621627

GI number: 116621627

Start: 3182265

End: 3184553

Strand: Reverse

Name: pbpG [H]

Synonym: Acid_2509

Alternate gene names: 116621627

Gene position: 3184553-3182265 (Counterclockwise)

Preceding gene: 116621628

Following gene: 116621625

Centisome position: 31.96

GC content: 62.21

Gene sequence:

>2289_bases
ATGCTCGCCACACCACAAAACCCCGAGCAGATCACGCTCCCGTTCTATCGCCGCCGTGGGGTGCGCATCGCCGCTGCATC
AATCCTGGGTCTCTGTCTGCTCTGCCTTGCGACTTTCATTTTTTACTACATAAAACTGGCGCACATAATCGATCAACGTC
TGGCATATGGACCTTTCGCTGGCACCACGAACATCTTCAGCGCCTCGCATCGCATCGCAGTCGGAGACCCACTCACACCC
GAAGAATTCACCGCTCATTTAGTGCGCAGCGGCTACACCAGCAGTCCGAATAATCCTGAGGGCTGGTATCTCCTCCATAA
GAACACCGTGGAGGTGAAGTCACCTGGCGGCCCGCTCGTAGTCGAATTCGGCAAAGGCAAAATCGCGGCTATCTCCGCGC
CCGGCCGCGCCGCCGTCTCCGAATTCGAGCTCGGTCCCGAGCTTATCGCGAACCTTTCCGGTAATCGCGAACGGCGCCGC
CTGGTGAAATTTGCCGACATTCCGCCCTCCGTGGTCCATGCCCTCGTCTCCGTCGAGGACAAGCGATTCTTCGAGCACTC
CGGCATCGATTTCCGCCGGTGCCTGAAGGCCGCCTACGTGGATCTGCGCAGCGGCCACAAAGATCAGGGCGCTTCCACGC
TCAGCATGCAACTGGCCCGCAGTTTTTGGCTGGAGCCCGACAAGCGCTGGAAGCGCAAGCTCGAGGAGTTGGTAATGACG
CTCCACATGGAGCGCAAGCTCACCAAGCAGCAGATCTTCGAATATTACGCCAACCAGGTCTACCTGGGCCGCCGCGATAC
CTTCAACATCAGCGGTTTCGCCGAAGGCGCCCGCGCGTACTTCGACAAGGATCTTTCCGCCATCGATACCTCTGAAGCGG
CATTTCTTGCCGGCTTGGTGCAGCGCCCCAGCTACTACAATCCCTACCGCTACCCCGAGCGCGCTCGCGAACGCCGCGAT
CTCGTCCTCCTGCTCATGCGGCGCAACCACTACCTGTCGGACGCCGAGTATCAGGCGGCGCTTCATGCTCCCCTGCGCTT
CGCGCCCGAACGACTGAACACTCACCAGAACCAGTACTTTGTCGATCTGGTGCGTTACGACCTGCGCAACCATCTCGAGG
AAACCGATCGCGAGGGGCGCAACATTTACACCACGCTAGATCCTGATCTGCAAGAAGCTGCCGAGGCCGCGGTCCGCATC
GGAATCGAAAAAGTGGATCGCCTCCTGCACTCCCATGGCGGCGAGCCGCAGGTCGCTCTGATCGCGCTCGATCCTCACAC
GGGAGAGGTCAAGGCCCTCGTCGGCGGCCGCGACTATGGACAAAGCCAGCTCAACCACGTCCTCGCCATGCGCCAGCCCG
GCTCCGTCTTCAAGCCTTTTGTGTACGCGGCGGCGTTGAACACCGCGGTCGCCGGCGGCCATGAGATCTTCACCCCGACG
AGCGTGGTCAGCGATGAGCCCGCCACCTTCTATTCCGGCAATCAGGTCTACCAACCCGGAAACTTCCACCACGAATTTAT
GGGTGACGTTACCTTGCGCGACGCTCTTGCCCATTCTCTGAACGTGGCCACCGTGACCCTGGCATCCGAGGTGGGCTATC
GCAATGTTGTGCAGATGGCCCAGCGCTCCGGCCTCAATAACGCGATCCGCGCGACCCCCTCAGTCGCTCTCGGGGCGTAT
GAAACCACCCCGCTCGAAATCGCCGGCGCGTACACTACCTTCGCGAATCAGGGCGTCAACGTGAAGCCTGCCAGCGTGTC
GTATGTGACCACCTCCGATGGCCGGACCATCTATCGGAACTCGCCCGAAAGCCACGCCGCGCTCGACCCGCGCGTCAACT
ATCTGCTGGTCAGCATGCTGCAGGACGTCCTCCGCATGGGTACCGGCGCAGGCGTCCGCGCACGCGGATTTACTTTGCCC
GCGGCCGGCAAGACCGGGACCTCCCGCGACGGCTGGTTCGCCGGCTTTACCAGCCAGCTCCTCTGCGTAGTCTGGGTCGG
CTACGATGATAACCGCGAACTGAACCTCGAAGGCGCTCGCTCGGCCCTCCCCGTCTGGGCCGAATTCATGACCCGGGCCG
CGAAGTTCAAACCCTATCGGGACGCCAAGGCGTTCGCGCCGCCCTCCGGCATCGTCACGGTACCGGTCTGTGAGGAATCG
GGCCAGATGGCCGGCCCCTTCTGCCCGCGCACGCGTTCGGACGTCTTCATCGACGGCACCCAGCCCCAGGCCCAGTGTTC
CCTGCACGACCCACGCATCGCCGCCGACCCCATTCCGGTAATCCGGTAG

Upstream 100 bases:

>100_bases
GTCCAGTAGGACGGGCCTCCTGGCCGATCGTGCTCTAAGGTTCTCCCACGCACAAATCTTCCGCCGGCATGGCCCCCTGC
TTGCTCCTACCTTTCACCAG

Downstream 100 bases:

>100_bases
CCGCAACCGCCGGCGTATCCTATCCCCCGGCGTGCTTAAAATATTGCACTGCCTTCTCGTGCTTCTCCGCCGCTTCCCTG
GTTTTGAATGTCCCCAAGTT

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 762; Mature: 762

Protein sequence:

>762_residues
MLATPQNPEQITLPFYRRRGVRIAAASILGLCLLCLATFIFYYIKLAHIIDQRLAYGPFAGTTNIFSASHRIAVGDPLTP
EEFTAHLVRSGYTSSPNNPEGWYLLHKNTVEVKSPGGPLVVEFGKGKIAAISAPGRAAVSEFELGPELIANLSGNRERRR
LVKFADIPPSVVHALVSVEDKRFFEHSGIDFRRCLKAAYVDLRSGHKDQGASTLSMQLARSFWLEPDKRWKRKLEELVMT
LHMERKLTKQQIFEYYANQVYLGRRDTFNISGFAEGARAYFDKDLSAIDTSEAAFLAGLVQRPSYYNPYRYPERARERRD
LVLLLMRRNHYLSDAEYQAALHAPLRFAPERLNTHQNQYFVDLVRYDLRNHLEETDREGRNIYTTLDPDLQEAAEAAVRI
GIEKVDRLLHSHGGEPQVALIALDPHTGEVKALVGGRDYGQSQLNHVLAMRQPGSVFKPFVYAAALNTAVAGGHEIFTPT
SVVSDEPATFYSGNQVYQPGNFHHEFMGDVTLRDALAHSLNVATVTLASEVGYRNVVQMAQRSGLNNAIRATPSVALGAY
ETTPLEIAGAYTTFANQGVNVKPASVSYVTTSDGRTIYRNSPESHAALDPRVNYLLVSMLQDVLRMGTGAGVRARGFTLP
AAGKTGTSRDGWFAGFTSQLLCVVWVGYDDNRELNLEGARSALPVWAEFMTRAAKFKPYRDAKAFAPPSGIVTVPVCEES
GQMAGPFCPRTRSDVFIDGTQPQAQCSLHDPRIAADPIPVIR

Sequences:

>Translated_762_residues
MLATPQNPEQITLPFYRRRGVRIAAASILGLCLLCLATFIFYYIKLAHIIDQRLAYGPFAGTTNIFSASHRIAVGDPLTP
EEFTAHLVRSGYTSSPNNPEGWYLLHKNTVEVKSPGGPLVVEFGKGKIAAISAPGRAAVSEFELGPELIANLSGNRERRR
LVKFADIPPSVVHALVSVEDKRFFEHSGIDFRRCLKAAYVDLRSGHKDQGASTLSMQLARSFWLEPDKRWKRKLEELVMT
LHMERKLTKQQIFEYYANQVYLGRRDTFNISGFAEGARAYFDKDLSAIDTSEAAFLAGLVQRPSYYNPYRYPERARERRD
LVLLLMRRNHYLSDAEYQAALHAPLRFAPERLNTHQNQYFVDLVRYDLRNHLEETDREGRNIYTTLDPDLQEAAEAAVRI
GIEKVDRLLHSHGGEPQVALIALDPHTGEVKALVGGRDYGQSQLNHVLAMRQPGSVFKPFVYAAALNTAVAGGHEIFTPT
SVVSDEPATFYSGNQVYQPGNFHHEFMGDVTLRDALAHSLNVATVTLASEVGYRNVVQMAQRSGLNNAIRATPSVALGAY
ETTPLEIAGAYTTFANQGVNVKPASVSYVTTSDGRTIYRNSPESHAALDPRVNYLLVSMLQDVLRMGTGAGVRARGFTLP
AAGKTGTSRDGWFAGFTSQLLCVVWVGYDDNRELNLEGARSALPVWAEFMTRAAKFKPYRDAKAFAPPSGIVTVPVCEES
GQMAGPFCPRTRSDVFIDGTQPQAQCSLHDPRIAADPIPVIR
>Mature_762_residues
MLATPQNPEQITLPFYRRRGVRIAAASILGLCLLCLATFIFYYIKLAHIIDQRLAYGPFAGTTNIFSASHRIAVGDPLTP
EEFTAHLVRSGYTSSPNNPEGWYLLHKNTVEVKSPGGPLVVEFGKGKIAAISAPGRAAVSEFELGPELIANLSGNRERRR
LVKFADIPPSVVHALVSVEDKRFFEHSGIDFRRCLKAAYVDLRSGHKDQGASTLSMQLARSFWLEPDKRWKRKLEELVMT
LHMERKLTKQQIFEYYANQVYLGRRDTFNISGFAEGARAYFDKDLSAIDTSEAAFLAGLVQRPSYYNPYRYPERARERRD
LVLLLMRRNHYLSDAEYQAALHAPLRFAPERLNTHQNQYFVDLVRYDLRNHLEETDREGRNIYTTLDPDLQEAAEAAVRI
GIEKVDRLLHSHGGEPQVALIALDPHTGEVKALVGGRDYGQSQLNHVLAMRQPGSVFKPFVYAAALNTAVAGGHEIFTPT
SVVSDEPATFYSGNQVYQPGNFHHEFMGDVTLRDALAHSLNVATVTLASEVGYRNVVQMAQRSGLNNAIRATPSVALGAY
ETTPLEIAGAYTTFANQGVNVKPASVSYVTTSDGRTIYRNSPESHAALDPRVNYLLVSMLQDVLRMGTGAGVRARGFTLP
AAGKTGTSRDGWFAGFTSQLLCVVWVGYDDNRELNLEGARSALPVWAEFMTRAAKFKPYRDAKAFAPPSGIVTVPVCEES
GQMAGPFCPRTRSDVFIDGTQPQAQCSLHDPRIAADPIPVIR

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=560, Percent_Identity=32.5, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1788867, Length=602, Percent_Identity=27.906976744186, Blast_Score=167, Evalue=3e-42,
Organism=Escherichia coli, GI87082258, Length=284, Percent_Identity=36.9718309859155, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=30.9090909090909, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 84501; Mature: 84501

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATPQNPEQITLPFYRRRGVRIAAASILGLCLLCLATFIFYYIKLAHIIDQRLAYGPFA
CCCCCCCCCEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GTTNIFSASHRIAVGDPLTPEEFTAHLVRSGYTSSPNNPEGWYLLHKNTVEVKSPGGPLV
CCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEECCCCCEE
VEFGKGKIAAISAPGRAAVSEFELGPELIANLSGNRERRRLVKFADIPPSVVHALVSVED
EEECCCEEEEEECCCCHHHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
KRFFEHSGIDFRRCLKAAYVDLRSGHKDQGASTLSMQLARSFWLEPDKRWKRKLEELVMT
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LHMERKLTKQQIFEYYANQVYLGRRDTFNISGFAEGARAYFDKDLSAIDTSEAAFLAGLV
HHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHH
QRPSYYNPYRYPERARERRDLVLLLMRRNHYLSDAEYQAALHAPLRFAPERLNTHQNQYF
HCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCHHHHHHHCCHHHCHHHHCCCCCHHH
VDLVRYDLRNHLEETDREGRNIYTTLDPDLQEAAEAAVRIGIEKVDRLLHSHGGEPQVAL
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
IALDPHTGEVKALVGGRDYGQSQLNHVLAMRQPGSVFKPFVYAAALNTAVAGGHEIFTPT
EEECCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECCCC
SVVSDEPATFYSGNQVYQPGNFHHEFMGDVTLRDALAHSLNVATVTLASEVGYRNVVQMA
HHCCCCCCEEECCCEEECCCCCCHHHHCCHHHHHHHHHHCCEEEEEEHHHCCHHHHHHHH
QRSGLNNAIRATPSVALGAYETTPLEIAGAYTTFANQGVNVKPASVSYVTTSDGRTIYRN
HHCCCCCHHHCCCCEEECCCCCCCEEEEEHHHHHHCCCCCCCCCEEEEEEECCCCEEECC
SPESHAALDPRVNYLLVSMLQDVLRMGTGAGVRARGFTLPAAGKTGTSRDGWFAGFTSQL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCEECCCCCCCCCCCCCCCHHHHHCE
LCVVWVGYDDNRELNLEGARSALPVWAEFMTRAAKFKPYRDAKAFAPPSGIVTVPVCEES
EEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCC
GQMAGPFCPRTRSDVFIDGTQPQAQCSLHDPRIAADPIPVIR
CCCCCCCCCCCCCCEEEECCCCCCEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLATPQNPEQITLPFYRRRGVRIAAASILGLCLLCLATFIFYYIKLAHIIDQRLAYGPFA
CCCCCCCCCEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GTTNIFSASHRIAVGDPLTPEEFTAHLVRSGYTSSPNNPEGWYLLHKNTVEVKSPGGPLV
CCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEECCCCCEE
VEFGKGKIAAISAPGRAAVSEFELGPELIANLSGNRERRRLVKFADIPPSVVHALVSVED
EEECCCEEEEEECCCCHHHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
KRFFEHSGIDFRRCLKAAYVDLRSGHKDQGASTLSMQLARSFWLEPDKRWKRKLEELVMT
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LHMERKLTKQQIFEYYANQVYLGRRDTFNISGFAEGARAYFDKDLSAIDTSEAAFLAGLV
HHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHH
QRPSYYNPYRYPERARERRDLVLLLMRRNHYLSDAEYQAALHAPLRFAPERLNTHQNQYF
HCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCHHHHHHHCCHHHCHHHHCCCCCHHH
VDLVRYDLRNHLEETDREGRNIYTTLDPDLQEAAEAAVRIGIEKVDRLLHSHGGEPQVAL
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
IALDPHTGEVKALVGGRDYGQSQLNHVLAMRQPGSVFKPFVYAAALNTAVAGGHEIFTPT
EEECCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECCCC
SVVSDEPATFYSGNQVYQPGNFHHEFMGDVTLRDALAHSLNVATVTLASEVGYRNVVQMA
HHCCCCCCEEECCCEEECCCCCCHHHHCCHHHHHHHHHHCCEEEEEEHHHCCHHHHHHHH
QRSGLNNAIRATPSVALGAYETTPLEIAGAYTTFANQGVNVKPASVSYVTTSDGRTIYRN
HHCCCCCHHHCCCCEEECCCCCCCEEEEEHHHHHHCCCCCCCCCEEEEEEECCCCEEECC
SPESHAALDPRVNYLLVSMLQDVLRMGTGAGVRARGFTLPAAGKTGTSRDGWFAGFTSQL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCEECCCCCCCCCCCCCCCHHHHHCE
LCVVWVGYDDNRELNLEGARSALPVWAEFMTRAAKFKPYRDAKAFAPPSGIVTVPVCEES
EEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCC
GQMAGPFCPRTRSDVFIDGTQPQAQCSLHDPRIAADPIPVIR
CCCCCCCCCCCCCCEEEECCCCCCEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]