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The map label for this gene is psdht [H]

Identifier: 116621619

GI number: 116621619

Start: 3175353

End: 3176270

Strand: Direct

Name: psdht [H]

Synonym: Acid_2501

Alternate gene names: 116621619

Gene position: 3175353-3176270 (Clockwise)

Preceding gene: 116621618

Following gene: 116621620

Centisome position: 31.86

GC content: 67.1

Gene sequence:

>918_bases
ATGAAGGAACGAGTGTTGGCGGCGTACGAGAGGATTCGGGGGACGGTGCGGGAGACTCCGCTGGAGCGGGCGGCGGGGTG
CGAAGCGTGGTTGAAGTTGGAGCACTTGCAGCACACCGGGAGCTTTAAGTTTCGCGGGGCTGCGAATAAGATCGGGCTGC
TGGCCGGGGCGCAGGAAGTGGTGACGGCGAGCAACGGGAATCACGGGCTCGGAGTGGCGGCGGCGGCGGAGGCGCGGGGG
ATTGCGGCGGAGGTGTTCGTGTCGTCCCACGTGTCGCGGGCCAAGGCGGCGCGGATTCAGGCGCGAGGAGCGCGGATTTG
CTACGCGGGGGACGATCCGCTGACGGCGGAGATGGCGGCGCGGCGGGCGGCGGAGGAGACGGGGCGGGTGTATATATCGC
CGTACAACGATCTGGACGTCGCGGCGGGGCAGGGAACGATCGCGGTGGAACTGCTGCGCCAGGCGCCGGATCTGGACGCG
GTGTTCGTGGCGGTCGGCGGGGGCGGACTGATCGGGGGGATCGGCTCGTACTTGAAGGCGATGTCGCCGCGGACGGAAGT
GGTGGGGTGCTGGCCGGAGAATTCGCCGGTGATGAAGGCGTGCCTGGAAGCGGGGAAGATCGTGGAGGTAGCAGAGCGGC
CGACGCTCTCCGAGAGCACGGCGGGCGGGTTGGAGGAAGGCTCGGTGACGTTCGAATTGTGCAGGCAGTCGATCGACCGG
AGCGTGCTGGTGACGGAAGAGGAGATCCTGGGCGCGATGCGGTTCGTGCTGCAGACGGAGCACTGGTTGATCGAAGGGGC
GGCCGGAGTGGCGATGGCGGCGTATCTCAAAGATGCGAAGCGCTATCACGGGAGGAAGGCGGCGGTGGTGCTTTGCGGGC
GCAATGTTTCGGAGGCGGTGCTGGAGAAAATATGTTGA

Upstream 100 bases:

>100_bases
ACGACATCTATCTGGGGGTCAAGTAAAGTCAAAGGCATGTCTCGCAAAGGCGCAAAGGACGCAAAGAAAACCTAAGAAAA
AGCAGGTGGTGAGGAGCGGA

Downstream 100 bases:

>100_bases
GGATCGCGCATCGACGGCGAAAACGGCGGTGGATCACACGAAAGAGCGGTGAGATACTATGCTCGTGAAAACTTTATGTC
TGATCGCGCTGATCGGCACG

Product: hypothetical protein

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKERVLAAYERIRGTVRETPLERAAGCEAWLKLEHLQHTGSFKFRGAANKIGLLAGAQEVVTASNGNHGLGVAAAAEARG
IAAEVFVSSHVSRAKAARIQARGARICYAGDDPLTAEMAARRAAEETGRVYISPYNDLDVAAGQGTIAVELLRQAPDLDA
VFVAVGGGGLIGGIGSYLKAMSPRTEVVGCWPENSPVMKACLEAGKIVEVAERPTLSESTAGGLEEGSVTFELCRQSIDR
SVLVTEEEILGAMRFVLQTEHWLIEGAAGVAMAAYLKDAKRYHGRKAAVVLCGRNVSEAVLEKIC

Sequences:

>Translated_305_residues
MKERVLAAYERIRGTVRETPLERAAGCEAWLKLEHLQHTGSFKFRGAANKIGLLAGAQEVVTASNGNHGLGVAAAAEARG
IAAEVFVSSHVSRAKAARIQARGARICYAGDDPLTAEMAARRAAEETGRVYISPYNDLDVAAGQGTIAVELLRQAPDLDA
VFVAVGGGGLIGGIGSYLKAMSPRTEVVGCWPENSPVMKACLEAGKIVEVAERPTLSESTAGGLEEGSVTFELCRQSIDR
SVLVTEEEILGAMRFVLQTEHWLIEGAAGVAMAAYLKDAKRYHGRKAAVVLCGRNVSEAVLEKIC
>Mature_305_residues
MKERVLAAYERIRGTVRETPLERAAGCEAWLKLEHLQHTGSFKFRGAANKIGLLAGAQEVVTASNGNHGLGVAAAAEARG
IAAEVFVSSHVSRAKAARIQARGARICYAGDDPLTAEMAARRAAEETGRVYISPYNDLDVAAGQGTIAVELLRQAPDLDA
VFVAVGGGGLIGGIGSYLKAMSPRTEVVGCWPENSPVMKACLEAGKIVEVAERPTLSESTAGGLEEGSVTFELCRQSIDR
SVLVTEEEILGAMRFVLQTEHWLIEGAAGVAMAAYLKDAKRYHGRKAAVVLCGRNVSEAVLEKIC

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=307, Percent_Identity=35.8306188925081, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI295821202, Length=285, Percent_Identity=27.0175438596491, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI295821200, Length=285, Percent_Identity=27.0175438596491, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4557415, Length=285, Percent_Identity=27.0175438596491, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789505, Length=323, Percent_Identity=30.9597523219814, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790207, Length=290, Percent_Identity=31.0344827586207, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71991565, Length=301, Percent_Identity=32.5581395348837, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17508781, Length=311, Percent_Identity=27.9742765273312, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17537387, Length=304, Percent_Identity=31.5789473684211, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322631, Length=302, Percent_Identity=31.1258278145695, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320930, Length=284, Percent_Identity=31.3380281690141, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319788, Length=284, Percent_Identity=23.943661971831, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355833, Length=304, Percent_Identity=32.8947368421053, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24645328, Length=211, Percent_Identity=32.7014218009479, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 32319; Mature: 32319

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERVLAAYERIRGTVRETPLERAAGCEAWLKLEHLQHTGSFKFRGAANKIGLLAGAQEV
CCCHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCHHH
VTASNGNHGLGVAAAAEARGIAAEVFVSSHVSRAKAARIQARGARICYAGDDPLTAEMAA
EEECCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
RRAAEETGRVYISPYNDLDVAAGQGTIAVELLRQAPDLDAVFVAVGGGGLIGGIGSYLKA
HHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
MSPRTEVVGCWPENSPVMKACLEAGKIVEVAERPTLSESTAGGLEEGSVTFELCRQSIDR
CCCCCCEEEECCCCCHHHHHHHHCCCEEEHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
SVLVTEEEILGAMRFVLQTEHWLIEGAAGVAMAAYLKDAKRYHGRKAAVVLCGRNVSEAV
EEEEEHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHH
LEKIC
HHHCC
>Mature Secondary Structure
MKERVLAAYERIRGTVRETPLERAAGCEAWLKLEHLQHTGSFKFRGAANKIGLLAGAQEV
CCCHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCHHH
VTASNGNHGLGVAAAAEARGIAAEVFVSSHVSRAKAARIQARGARICYAGDDPLTAEMAA
EEECCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
RRAAEETGRVYISPYNDLDVAAGQGTIAVELLRQAPDLDAVFVAVGGGGLIGGIGSYLKA
HHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
MSPRTEVVGCWPENSPVMKACLEAGKIVEVAERPTLSESTAGGLEEGSVTFELCRQSIDR
CCCCCCEEEECCCCCHHHHHHHHCCCEEEHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
SVLVTEEEILGAMRFVLQTEHWLIEGAAGVAMAAYLKDAKRYHGRKAAVVLCGRNVSEAV
EEEEEHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHH
LEKIC
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]