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The map label for this gene is pbpE [H]

Identifier: 116621608

GI number: 116621608

Start: 3158406

End: 3160094

Strand: Reverse

Name: pbpE [H]

Synonym: Acid_2490

Alternate gene names: 116621608

Gene position: 3160094-3158406 (Counterclockwise)

Preceding gene: 116621611

Following gene: 116621606

Centisome position: 31.71

GC content: 62.23

Gene sequence:

>1689_bases
ATGCATTACCGCTTTCCCCTCCTCGCTCTGCTTGCGGGCTCCTGCTTCGCGCAGGACGCCGCGCGGATGAATGAAATCGT
CCAATCCTATGTGGCCAACCACCAGTTCATGGGAACCGCCCTGGTCGCGCGCGGCGGTCAGATTCTCTTCAGCAAGGGCT
ACGGCTCCGCCAACCTGGAGTGGGATGTCCCCAATTCACCGGCCACCAAGTTCCGTCTCGGCTCCGTCACCAAGCAGTTC
ACCGCCGCCTCCATCCTTCTGCTTGAAGAACGCGGCAAGATCAAAACCGGCGACCCCGTCAAAACCTATCTCCCCGACGC
CCCCGCCGCCTGGGACAAGATCACCATATTTAACCTGCTCACCCACACCTCCGGCATTCCCAACTTCACCGGCTTCCCCG
AATACGGCAAGCTCGAGCCCTTCGCCGCTACTCCCGCCGAGCTCGTCGCGCGCTTCCGCGACAAGCCCCTCGATTTCCAA
CCCGGGGAAAAGTGGAACTACAGCAACTCGGGCTATGTCCTGCTCGGCTACCTGATCGAAAAGATCACCGGCGGAACCTA
TGAGAAATTCGTGCGCGAAAATATCTTCACTCCCCTGGGCATGATGGATTCCGGCTACGATTCCAACTCCGCCATCATCC
CTCGACGCGCCTCCGGCTACGTCAATGGCAGGAACGGCATGGAAAACGCCGGCTTCATCAACATGACCATCCCGCATGGC
GCGGGCGCCCTCTACTCCACTACCGAAGACCTCTTGAAATGGCAGCAGGGCCTCTTCGGCGGAAAGGTGCTGCAGCCCGC
CTCGCTCGAAAAAATGACCACGCCCTTCAAGAACAACTATGCCTGCGGCGTCGGCGTGGACACGGTCGCCGGACGCAAGG
TGATCTCCCACGGCGGCGGCATCGAGGGCTTCAATACTCAGCTCGAGTATTACCCCGACGACAAGCTCACCGTCGTAGTC
CTCGGCAACCTGAATGGACCCGCCCCCGGCGAGATCGCCAAAAAGCTTGCCGCGCAAGCCCACGGCGAAGCCGTGAAGCT
GACCACCGAACGCAAGGAAATCACGCTCGATCCCAAACAGCTCGCCCGCTACGTCGGCGCCTATCAAATGACCGGCGGCC
CCGCTATGTTGGTCACGCTTGAAGGCAATCAACTGCTCAGCAAGTTAGGCAATCAGAACCCGGTGAGTATCTTCCCGGAA
TCGCCCACTCTCTTCTTCCTGAAGGTGGTCGATGCCCAGCTCGAGTTCGCCATGCCCGATCCGCAGTCCCCGCCCAGCAA
GGTGACGCTCCACCAGAACGGCCGCGAGATCGTCGGCAAGCGCCTCGATGAGGCCGCGTCAAAACGCCTCGCCGACGCCG
CCGCTGCGACCGCCAAACGGATTAAGGACCAGACCGCCGCACCCGGCAGCGAAGCCGCCCTCCGCAAAATGATCCAGGAC
GTGCGCCTCGGCAAACCCGACTACGCCGGCATGAGTTCCGGCCTTGCCGACGCGACCCGCCAGCAGCTCCCCCAGCTCCA
GTCCGACATCGCCAGACTCGGCGCGCTCCAGTCCCTGACCTTCAAGTCAGTCGGCCCCGCCGGCCCCGACATCTACCAGG
CCAAATTCGAAAACGGCTCCCTCGAATACCGCATCTGGCTCGGTCCCGACGGCAAAATCGACGCCGCCAACGCCCGCCCG
ATCCAGTAG

Upstream 100 bases:

>100_bases
ATACTCAGTTCGCTAGGGTAGTGATCTGATTGAATCATGCCCGCGCCGCGCTGTCAAGGCTCCGCTTGCTCCCCTTCTTC
AAACGCGATATACCAGATCC

Downstream 100 bases:

>100_bases
GACAGCCTCCCGGCCTGTCCCTTTAGTCTGTTTTTTGTGGGGCCGGCGTCTACGCACACGGACTCCGTCCCGCGAACCGC
GACCGTCAGGGAGCGATCTC

Product: beta-lactamase

Products: NA

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MHYRFPLLALLAGSCFAQDAARMNEIVQSYVANHQFMGTALVARGGQILFSKGYGSANLEWDVPNSPATKFRLGSVTKQF
TAASILLLEERGKIKTGDPVKTYLPDAPAAWDKITIFNLLTHTSGIPNFTGFPEYGKLEPFAATPAELVARFRDKPLDFQ
PGEKWNYSNSGYVLLGYLIEKITGGTYEKFVRENIFTPLGMMDSGYDSNSAIIPRRASGYVNGRNGMENAGFINMTIPHG
AGALYSTTEDLLKWQQGLFGGKVLQPASLEKMTTPFKNNYACGVGVDTVAGRKVISHGGGIEGFNTQLEYYPDDKLTVVV
LGNLNGPAPGEIAKKLAAQAHGEAVKLTTERKEITLDPKQLARYVGAYQMTGGPAMLVTLEGNQLLSKLGNQNPVSIFPE
SPTLFFLKVVDAQLEFAMPDPQSPPSKVTLHQNGREIVGKRLDEAASKRLADAAAATAKRIKDQTAAPGSEAALRKMIQD
VRLGKPDYAGMSSGLADATRQQLPQLQSDIARLGALQSLTFKSVGPAGPDIYQAKFENGSLEYRIWLGPDGKIDAANARP
IQ

Sequences:

>Translated_562_residues
MHYRFPLLALLAGSCFAQDAARMNEIVQSYVANHQFMGTALVARGGQILFSKGYGSANLEWDVPNSPATKFRLGSVTKQF
TAASILLLEERGKIKTGDPVKTYLPDAPAAWDKITIFNLLTHTSGIPNFTGFPEYGKLEPFAATPAELVARFRDKPLDFQ
PGEKWNYSNSGYVLLGYLIEKITGGTYEKFVRENIFTPLGMMDSGYDSNSAIIPRRASGYVNGRNGMENAGFINMTIPHG
AGALYSTTEDLLKWQQGLFGGKVLQPASLEKMTTPFKNNYACGVGVDTVAGRKVISHGGGIEGFNTQLEYYPDDKLTVVV
LGNLNGPAPGEIAKKLAAQAHGEAVKLTTERKEITLDPKQLARYVGAYQMTGGPAMLVTLEGNQLLSKLGNQNPVSIFPE
SPTLFFLKVVDAQLEFAMPDPQSPPSKVTLHQNGREIVGKRLDEAASKRLADAAAATAKRIKDQTAAPGSEAALRKMIQD
VRLGKPDYAGMSSGLADATRQQLPQLQSDIARLGALQSLTFKSVGPAGPDIYQAKFENGSLEYRIWLGPDGKIDAANARP
IQ
>Mature_562_residues
MHYRFPLLALLAGSCFAQDAARMNEIVQSYVANHQFMGTALVARGGQILFSKGYGSANLEWDVPNSPATKFRLGSVTKQF
TAASILLLEERGKIKTGDPVKTYLPDAPAAWDKITIFNLLTHTSGIPNFTGFPEYGKLEPFAATPAELVARFRDKPLDFQ
PGEKWNYSNSGYVLLGYLIEKITGGTYEKFVRENIFTPLGMMDSGYDSNSAIIPRRASGYVNGRNGMENAGFINMTIPHG
AGALYSTTEDLLKWQQGLFGGKVLQPASLEKMTTPFKNNYACGVGVDTVAGRKVISHGGGIEGFNTQLEYYPDDKLTVVV
LGNLNGPAPGEIAKKLAAQAHGEAVKLTTERKEITLDPKQLARYVGAYQMTGGPAMLVTLEGNQLLSKLGNQNPVSIFPE
SPTLFFLKVVDAQLEFAMPDPQSPPSKVTLHQNGREIVGKRLDEAASKRLADAAAATAKRIKDQTAAPGSEAALRKMIQD
VRLGKPDYAGMSSGLADATRQQLPQLQSDIARLGALQSLTFKSVGPAGPDIYQAKFENGSLEYRIWLGPDGKIDAANARP
IQ

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI239747261, Length=311, Percent_Identity=23.1511254019293, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI239752721, Length=311, Percent_Identity=23.1511254019293, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI239741085, Length=311, Percent_Identity=23.1511254019293, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI26051231, Length=196, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI87082101, Length=339, Percent_Identity=24.188790560472, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1790593, Length=379, Percent_Identity=25.065963060686, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1786574, Length=332, Percent_Identity=26.2048192771084, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533026, Length=368, Percent_Identity=25, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17550934, Length=201, Percent_Identity=35.8208955223881, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17565630, Length=190, Percent_Identity=28.4210526315789, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: NA

Molecular weight: Translated: 60844; Mature: 60844

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYRFPLLALLAGSCFAQDAARMNEIVQSYVANHQFMGTALVARGGQILFSKGYGSANLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCEE
WDVPNSPATKFRLGSVTKQFTAASILLLEERGKIKTGDPVKTYLPDAPAAWDKITIFNLL
EECCCCCCCEEEHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHCCCCCCCCCHHHHHHHH
THTSGIPNFTGFPEYGKLEPFAATPAELVARFRDKPLDFQPGEKWNYSNSGYVLLGYLIE
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHH
KITGGTYEKFVRENIFTPLGMMDSGYDSNSAIIPRRASGYVNGRNGMENAGFINMTIPHG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEECCCC
AGALYSTTEDLLKWQQGLFGGKVLQPASLEKMTTPFKNNYACGVGVDTVAGRKVISHGGG
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEECCCCHHHHCHHHHHCCCC
IEGFNTQLEYYPDDKLTVVVLGNLNGPAPGEIAKKLAAQAHGEAVKLTTERKEITLDPKQ
CCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEECHHH
LARYVGAYQMTGGPAMLVTLEGNQLLSKLGNQNPVSIFPESPTLFFLKVVDAQLEFAMPD
HHHHHCCEEECCCCEEEEEECCHHHHHHHCCCCCEEEECCCCCEEEEEEECCHHEECCCC
PQSPPSKVTLHQNGREIVGKRLDEAASKRLADAAAATAKRIKDQTAAPGSEAALRKMIQD
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VRLGKPDYAGMSSGLADATRQQLPQLQSDIARLGALQSLTFKSVGPAGPDIYQAKFENGS
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
LEYRIWLGPDGKIDAANARPIQ
EEEEEEECCCCCEECCCCCCCC
>Mature Secondary Structure
MHYRFPLLALLAGSCFAQDAARMNEIVQSYVANHQFMGTALVARGGQILFSKGYGSANLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCEE
WDVPNSPATKFRLGSVTKQFTAASILLLEERGKIKTGDPVKTYLPDAPAAWDKITIFNLL
EECCCCCCCEEEHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHCCCCCCCCCHHHHHHHH
THTSGIPNFTGFPEYGKLEPFAATPAELVARFRDKPLDFQPGEKWNYSNSGYVLLGYLIE
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHH
KITGGTYEKFVRENIFTPLGMMDSGYDSNSAIIPRRASGYVNGRNGMENAGFINMTIPHG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEECCCC
AGALYSTTEDLLKWQQGLFGGKVLQPASLEKMTTPFKNNYACGVGVDTVAGRKVISHGGG
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEEECCCCHHHHCHHHHHCCCC
IEGFNTQLEYYPDDKLTVVVLGNLNGPAPGEIAKKLAAQAHGEAVKLTTERKEITLDPKQ
CCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEECHHH
LARYVGAYQMTGGPAMLVTLEGNQLLSKLGNQNPVSIFPESPTLFFLKVVDAQLEFAMPD
HHHHHCCEEECCCCEEEEEECCHHHHHHHCCCCCEEEECCCCCEEEEEEECCHHEECCCC
PQSPPSKVTLHQNGREIVGKRLDEAASKRLADAAAATAKRIKDQTAAPGSEAALRKMIQD
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VRLGKPDYAGMSSGLADATRQQLPQLQSDIARLGALQSLTFKSVGPAGPDIYQAKFENGS
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
LEYRIWLGPDGKIDAANARPIQ
EEEEEEECCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]