Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 116621556

GI number: 116621556

Start: 3100216

End: 3101529

Strand: Reverse

Name: purB [H]

Synonym: Acid_2438

Alternate gene names: 116621556

Gene position: 3101529-3100216 (Counterclockwise)

Preceding gene: 116621557

Following gene: 116621555

Centisome position: 31.12

GC content: 64.69

Gene sequence:

>1314_bases
ATGATTTCTCGATACACGCGTCCCGAAATGGGGCGGATCTGGAGCGACCAGAACAAATTTCAACAGTGGCTGGAAGTCGA
ACTGGCCGCCTCCGAAGCGCTCGCCGAGCTCGGTGTTGTACCCGCCGGGGCCGCGCGCCTGTTACGCGAACACGCCGGGT
TCGACGTGGCGCGCATCCACGAGATCGAAAAGGAAGTGAAGCACGACGTGATCGCCTTCACCACCGCGGTCGCCGAAACC
ATGAAGGCGCGCGGCCATGCCGAGGCCTCGCGCTGGTTCCACTACGGGCTGACCTCGAACGACGTGGTGGATACCGCGCA
GGCGCTGTGCCTGCAACAGGCGAGCGCGATCCTGCTGAAGGACCTGGCCGGACTGCGCGAGGTGCTGAAGCGGCGCGCGC
TGGAATTCCAGCACACGGTGCAGATCGGGCGCACGCACGGCGTGCACGCGGAGCCGATCACGTTCGGGCTGAAGCTGGCG
ATCTGGTACGAGGAAGCGGGGCGCAATCTGGCGCGGCTTTCGGCAGCGGCGGAGGACGTGCGGGTAGGTAAAACCTCGGG
CGCGGTGGGCACGTTCGGCCACATTGATCCCGAGACGGAGGAGATGATCTGCGCGCGGCTGAACCTGAAGCCGGCGCCGG
TGGCCTCGCAGGTGATCCAGCGCGACCGGCACGCGAATTTCGTGGCGGCGCTGGCTTTGACCACGGCGCTCTGCGAAAAG
ATCGCGCTGGAAGTACGGCACCTGCAGCGCACCGAGGTCCGCGAGGCGGAGGAGTATTTCGCGAAGGGGCAGAAGGGCAG
CTCCGCGATGCCGCATAAGCGGAATCCGGTGACGTGCGAGCAGATCTGCGGATTGGCGCGGGTGGTGCGCGGCAACGTGA
CGCCGGCGCTCGAAGATGTAGCCTTATGGCACGAGCGTGATATCTCTCATTCGTCGGTAGAGCGCGTGATTCTTCCCGAT
TCGACGATTCTGACTGACTACCTGCTCGAAAAGACCACGAAGCTGGTGGACCAGCTCCTGGTCTACCCGGAGCGTATGCG
GCGCAACCTCGACCTGACGCGCGGACTGGTGTTCAGCGGGCAGCTGCTGCTGGATCTGGCCGCCGCCGGAATGCTGCGCG
AACAGGCTTACCGGCTGGTGCAATCGCACGCGATGCGGGCGTGGGAGGAAGAGGGTGACTTCCGCGCGGCAATCGAAGCG
GACCCGGATATCGCGGCGGTCCTCACCAGGGACAAGATCGCCGAGGCCTTCTCCCTGGAGCGTCAATTGCGGAATATCGA
CAAAATCTTCACCCGAGTGTTCGGCCGGACGTGA

Upstream 100 bases:

>100_bases
ATGGTGGCTACCGACGCGCTGGAGCAGCGCGGCCTGCGGACGCGGCTTTCGATTGCGGCGCAGCAGACCCCGAGCGAAGT
TTTTCAATTCTAAGACCATC

Downstream 100 bases:

>100_bases
GAAAGCGTTCCAACGTTTCACCCAAAGTGGTGCGTACTGAACGGCGAATGAGTGTACGATATCCTTTCGACATTTGCATA
TGGCAAGGATAACCGGCAAG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MISRYTRPEMGRIWSDQNKFQQWLEVELAASEALAELGVVPAGAARLLREHAGFDVARIHEIEKEVKHDVIAFTTAVAET
MKARGHAEASRWFHYGLTSNDVVDTAQALCLQQASAILLKDLAGLREVLKRRALEFQHTVQIGRTHGVHAEPITFGLKLA
IWYEEAGRNLARLSAAAEDVRVGKTSGAVGTFGHIDPETEEMICARLNLKPAPVASQVIQRDRHANFVAALALTTALCEK
IALEVRHLQRTEVREAEEYFAKGQKGSSAMPHKRNPVTCEQICGLARVVRGNVTPALEDVALWHERDISHSSVERVILPD
STILTDYLLEKTTKLVDQLLVYPERMRRNLDLTRGLVFSGQLLLDLAAAGMLREQAYRLVQSHAMRAWEEEGDFRAAIEA
DPDIAAVLTRDKIAEAFSLERQLRNIDKIFTRVFGRT

Sequences:

>Translated_437_residues
MISRYTRPEMGRIWSDQNKFQQWLEVELAASEALAELGVVPAGAARLLREHAGFDVARIHEIEKEVKHDVIAFTTAVAET
MKARGHAEASRWFHYGLTSNDVVDTAQALCLQQASAILLKDLAGLREVLKRRALEFQHTVQIGRTHGVHAEPITFGLKLA
IWYEEAGRNLARLSAAAEDVRVGKTSGAVGTFGHIDPETEEMICARLNLKPAPVASQVIQRDRHANFVAALALTTALCEK
IALEVRHLQRTEVREAEEYFAKGQKGSSAMPHKRNPVTCEQICGLARVVRGNVTPALEDVALWHERDISHSSVERVILPD
STILTDYLLEKTTKLVDQLLVYPERMRRNLDLTRGLVFSGQLLLDLAAAGMLREQAYRLVQSHAMRAWEEEGDFRAAIEA
DPDIAAVLTRDKIAEAFSLERQLRNIDKIFTRVFGRT
>Mature_437_residues
MISRYTRPEMGRIWSDQNKFQQWLEVELAASEALAELGVVPAGAARLLREHAGFDVARIHEIEKEVKHDVIAFTTAVAET
MKARGHAEASRWFHYGLTSNDVVDTAQALCLQQASAILLKDLAGLREVLKRRALEFQHTVQIGRTHGVHAEPITFGLKLA
IWYEEAGRNLARLSAAAEDVRVGKTSGAVGTFGHIDPETEEMICARLNLKPAPVASQVIQRDRHANFVAALALTTALCEK
IALEVRHLQRTEVREAEEYFAKGQKGSSAMPHKRNPVTCEQICGLARVVRGNVTPALEDVALWHERDISHSSVERVILPD
STILTDYLLEKTTKLVDQLLVYPERMRRNLDLTRGLVFSGQLLLDLAAAGMLREQAYRLVQSHAMRAWEEEGDFRAAIEA
DPDIAAVLTRDKIAEAFSLERQLRNIDKIFTRVFGRT

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=418, Percent_Identity=27.7511961722488, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI183227688, Length=406, Percent_Identity=27.8325123152709, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787376, Length=322, Percent_Identity=28.8819875776398, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508577, Length=431, Percent_Identity=26.4501160092807, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI32564234, Length=367, Percent_Identity=28.6103542234332, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323391, Length=417, Percent_Identity=28.0575539568345, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24647570, Length=435, Percent_Identity=26.4367816091954, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48891; Mature: 48891

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRYTRPEMGRIWSDQNKFQQWLEVELAASEALAELGVVPAGAARLLREHAGFDVARIH
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHH
EIEKEVKHDVIAFTTAVAETMKARGHAEASRWFHYGLTSNDVVDTAQALCLQQASAILLK
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHH
DLAGLREVLKRRALEFQHTVQIGRTHGVHAEPITFGLKLAIWYEEAGRNLARLSAAAEDV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEEEEHHHCCHHHHHHHHHHHH
RVGKTSGAVGTFGHIDPETEEMICARLNLKPAPVASQVIQRDRHANFVAALALTTALCEK
HCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALEVRHLQRTEVREAEEYFAKGQKGSSAMPHKRNPVTCEQICGLARVVRGNVTPALEDV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
ALWHERDISHSSVERVILPDSTILTDYLLEKTTKLVDQLLVYPERMRRNLDLTRGLVFSG
HHHHHCCCCHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHCC
QLLLDLAAAGMLREQAYRLVQSHAMRAWEEEGDFRAAIEADPDIAAVLTRDKIAEAFSLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RQLRNIDKIFTRVFGRT
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISRYTRPEMGRIWSDQNKFQQWLEVELAASEALAELGVVPAGAARLLREHAGFDVARIH
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHH
EIEKEVKHDVIAFTTAVAETMKARGHAEASRWFHYGLTSNDVVDTAQALCLQQASAILLK
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHH
DLAGLREVLKRRALEFQHTVQIGRTHGVHAEPITFGLKLAIWYEEAGRNLARLSAAAEDV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEEEEHHHCCHHHHHHHHHHHH
RVGKTSGAVGTFGHIDPETEEMICARLNLKPAPVASQVIQRDRHANFVAALALTTALCEK
HCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALEVRHLQRTEVREAEEYFAKGQKGSSAMPHKRNPVTCEQICGLARVVRGNVTPALEDV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
ALWHERDISHSSVERVILPDSTILTDYLLEKTTKLVDQLLVYPERMRRNLDLTRGLVFSG
HHHHHCCCCHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHCC
QLLLDLAAAGMLREQAYRLVQSHAMRAWEEEGDFRAAIEADPDIAAVLTRDKIAEAFSLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RQLRNIDKIFTRVFGRT
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]