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The map label for this gene is mltF [H]

Identifier: 116621511

GI number: 116621511

Start: 3047514

End: 3049082

Strand: Reverse

Name: mltF [H]

Synonym: Acid_2393

Alternate gene names: 116621511

Gene position: 3049082-3047514 (Counterclockwise)

Preceding gene: 116621514

Following gene: 116621510

Centisome position: 30.6

GC content: 58.32

Gene sequence:

>1569_bases
ATGAGTGATGCCCGCACCCTCCGGCAGGTTGTCGCCTGTGCGATGGTCGTTGCTGCTATTGCAGCCTGCCCGTCATGCGG
CAAACGTCCAGGTGAAACCCCATCCGCGCGGAAGGCCGGAAAGGCAGCGGCCGCCCCATCGGCCGGCAGTGCGTCGCGCT
TTTCGATCGAGCCCGAGACAAACACGTTCGAACACCCCGCTCTACAGGAGCAGCTCGTTCACGAACGCTGGCGCGGCGAT
CTCGACGGCATTGTCAGCCGGCGGATTCTGCGGGTTCTGGTAGCGCCCAACAAGCTCGGATTCTACTTCGATGGAAGCGA
GATTCGTGGCGCCCTCTATGAGTTCTGCCGCGAATTCGAGCACTTCCTGAATCGCAAGCTCCACACCGGCAATCTCGCCA
TTCACCTCGTCTTCGTCCCGATAGGCCGGGACAACCTGCTGCCCAAGCTGGCAGAAGGTTATGGCGACTTGGTCGCGACC
CTGATGGTCCGGTCGAACCAGCCTCAATATGCCGTGGATTTCACGGATTCCCTCTTCGATGACGCCAGGGCCGTCATCGT
CAGCGGTACCGGCGAGCAATTGTTGCGCATCGAGGATCTTTCCGGCCGCGAGGTCTACTACTTCAGCAATACGATTCCGT
ATGAAAACCTGCGACGTCTCAGTGCAGACTTCGTACGCGACGGCAAACCTCCGATTCACCTCATTCCCGCGGCTCCCGAC
TTACAAGTGGAGGACTTACTGGAGATGGTGAATGCCGGCCTCATACCCCTGACCCTCGCGGAAGACAAGCTTGCGCAGTA
CTGGGCCCAGGTGCTGCCCAATCTGAAGATCCAATCCCACTTGGTCGTGGCTGACAGTCCCTTGGCGTGGGCCGTGCAAC
AGAATACGCCCCGGCTCAAAGCCGTTATCAATGAATTCATTCGCGATCATAAGATCGGCACGCTTTACGGAAACACAGTA
AAAGATAAGTATTTGAGCCGCGTCCCCTGGGTAAAAGACGCAATTGCGGGAGAGGATCTCGCGCACTTTGAAGAAATGGT
GCGCCTGTTCCGCAAATACGGCGACAAATATCAATTCCCTTATCTGCTTCTGGCGGCGCAAGGCTATCAGGAATCCGGCC
TCAACCCTCGCCTGAGGAGCAGGGCCGGGGCTGTCGGCGTCATGCAGATCAAACCGAGCACTGCCGCCGCCGACCCCATC
GACATCACCGGCATTGAGAAAATCGATCGCAATATCGAGGCGGGAGCCAAATACCTCCGGCACATGGTGAGTCATTACTA
CGCCAAAGAGCCGATGGATCAGATCACCAGGGGTCTCTTCGCGCTGGCTTCGTACAACGCAGGCCCCGACAGAATCGAAA
GGCTCCGCTCCGTGGCTGCCGCCGAAGGCTACGATCCCAATCTCTGGTTCAACAATGTCGAGTTCATTGCCGCTCGCCGC
ATCGGCGCCGAAACAACGAACTATGTCAGCAACGTCTACAAGTACTACCTGGCATACAAAATGACCATCGAACAGGACGC
CCGCAGGCAGGCCTCGCGCTCGGGGGCTCTTCCCGGCAAAACTCGATAG

Upstream 100 bases:

>100_bases
CAAATTTGTCCCGCCCCGGAGGCGCATTTTGCGCAGCCCCAACTCTCGCCAACGTCAGGATTTAAGTCCGAAGCATCACC
TTCGCCATCGATCTTTATAG

Downstream 100 bases:

>100_bases
CCGCAACCGGGAGATTCGAAGCCCCATCCCTTCGGCCTGGTGTCAAACATGTGAACCAGCCAAGGAAGGAATCGCATCCA
GCACAGCATGGGAGTTCGGC

Product: lytic transglycosylase catalytic subunit subunit

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSDARTLRQVVACAMVVAAIAACPSCGKRPGETPSARKAGKAAAAPSAGSASRFSIEPETNTFEHPALQEQLVHERWRGD
LDGIVSRRILRVLVAPNKLGFYFDGSEIRGALYEFCREFEHFLNRKLHTGNLAIHLVFVPIGRDNLLPKLAEGYGDLVAT
LMVRSNQPQYAVDFTDSLFDDARAVIVSGTGEQLLRIEDLSGREVYYFSNTIPYENLRRLSADFVRDGKPPIHLIPAAPD
LQVEDLLEMVNAGLIPLTLAEDKLAQYWAQVLPNLKIQSHLVVADSPLAWAVQQNTPRLKAVINEFIRDHKIGTLYGNTV
KDKYLSRVPWVKDAIAGEDLAHFEEMVRLFRKYGDKYQFPYLLLAAQGYQESGLNPRLRSRAGAVGVMQIKPSTAAADPI
DITGIEKIDRNIEAGAKYLRHMVSHYYAKEPMDQITRGLFALASYNAGPDRIERLRSVAAAEGYDPNLWFNNVEFIAARR
IGAETTNYVSNVYKYYLAYKMTIEQDARRQASRSGALPGKTR

Sequences:

>Translated_522_residues
MSDARTLRQVVACAMVVAAIAACPSCGKRPGETPSARKAGKAAAAPSAGSASRFSIEPETNTFEHPALQEQLVHERWRGD
LDGIVSRRILRVLVAPNKLGFYFDGSEIRGALYEFCREFEHFLNRKLHTGNLAIHLVFVPIGRDNLLPKLAEGYGDLVAT
LMVRSNQPQYAVDFTDSLFDDARAVIVSGTGEQLLRIEDLSGREVYYFSNTIPYENLRRLSADFVRDGKPPIHLIPAAPD
LQVEDLLEMVNAGLIPLTLAEDKLAQYWAQVLPNLKIQSHLVVADSPLAWAVQQNTPRLKAVINEFIRDHKIGTLYGNTV
KDKYLSRVPWVKDAIAGEDLAHFEEMVRLFRKYGDKYQFPYLLLAAQGYQESGLNPRLRSRAGAVGVMQIKPSTAAADPI
DITGIEKIDRNIEAGAKYLRHMVSHYYAKEPMDQITRGLFALASYNAGPDRIERLRSVAAAEGYDPNLWFNNVEFIAARR
IGAETTNYVSNVYKYYLAYKMTIEQDARRQASRSGALPGKTR
>Mature_521_residues
SDARTLRQVVACAMVVAAIAACPSCGKRPGETPSARKAGKAAAAPSAGSASRFSIEPETNTFEHPALQEQLVHERWRGDL
DGIVSRRILRVLVAPNKLGFYFDGSEIRGALYEFCREFEHFLNRKLHTGNLAIHLVFVPIGRDNLLPKLAEGYGDLVATL
MVRSNQPQYAVDFTDSLFDDARAVIVSGTGEQLLRIEDLSGREVYYFSNTIPYENLRRLSADFVRDGKPPIHLIPAAPDL
QVEDLLEMVNAGLIPLTLAEDKLAQYWAQVLPNLKIQSHLVVADSPLAWAVQQNTPRLKAVINEFIRDHKIGTLYGNTVK
DKYLSRVPWVKDAIAGEDLAHFEEMVRLFRKYGDKYQFPYLLLAAQGYQESGLNPRLRSRAGAVGVMQIKPSTAAADPID
ITGIEKIDRNIEAGAKYLRHMVSHYYAKEPMDQITRGLFALASYNAGPDRIERLRSVAAAEGYDPNLWFNNVEFIAARRI
GAETTNYVSNVYKYYLAYKMTIEQDARRQASRSGALPGKTR

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=301, Percent_Identity=23.9202657807309, Blast_Score=62, Evalue=9e-11,
Organism=Escherichia coli, GI87082441, Length=192, Percent_Identity=28.6458333333333, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 58290; Mature: 58159

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDARTLRQVVACAMVVAAIAACPSCGKRPGETPSARKAGKAAAAPSAGSASRFSIEPET
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEECCCC
NTFEHPALQEQLVHERWRGDLDGIVSRRILRVLVAPNKLGFYFDGSEIRGALYEFCREFE
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHH
HFLNRKLHTGNLAIHLVFVPIGRDNLLPKLAEGYGDLVATLMVRSNQPQYAVDFTDSLFD
HHHCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHC
DARAVIVSGTGEQLLRIEDLSGREVYYFSNTIPYENLRRLSADFVRDGKPPIHLIPAAPD
CCCEEEEECCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCC
LQVEDLLEMVNAGLIPLTLAEDKLAQYWAQVLPNLKIQSHLVVADSPLAWAVQQNTPRLK
CCHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCEECCEEEEECCCCEEEECCCCCHHH
AVINEFIRDHKIGTLYGNTVKDKYLSRVPWVKDAIAGEDLAHFEEMVRLFRKYGDKYQFP
HHHHHHHHHCCCEEEECCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
YLLLAAQGYQESGLNPRLRSRAGAVGVMQIKPSTAAADPIDITGIEKIDRNIEAGAKYLR
EEEEEECCHHHCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHH
HMVSHYYAKEPMDQITRGLFALASYNAGPDRIERLRSVAAAEGYDPNLWFNNVEFIAARR
HHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
IGAETTNYVSNVYKYYLAYKMTIEQDARRQASRSGALPGKTR
CCCHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDARTLRQVVACAMVVAAIAACPSCGKRPGETPSARKAGKAAAAPSAGSASRFSIEPET
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEECCCC
NTFEHPALQEQLVHERWRGDLDGIVSRRILRVLVAPNKLGFYFDGSEIRGALYEFCREFE
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHH
HFLNRKLHTGNLAIHLVFVPIGRDNLLPKLAEGYGDLVATLMVRSNQPQYAVDFTDSLFD
HHHCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHC
DARAVIVSGTGEQLLRIEDLSGREVYYFSNTIPYENLRRLSADFVRDGKPPIHLIPAAPD
CCCEEEEECCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCC
LQVEDLLEMVNAGLIPLTLAEDKLAQYWAQVLPNLKIQSHLVVADSPLAWAVQQNTPRLK
CCHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCEECCEEEEECCCCEEEECCCCCHHH
AVINEFIRDHKIGTLYGNTVKDKYLSRVPWVKDAIAGEDLAHFEEMVRLFRKYGDKYQFP
HHHHHHHHHCCCEEEECCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
YLLLAAQGYQESGLNPRLRSRAGAVGVMQIKPSTAAADPIDITGIEKIDRNIEAGAKYLR
EEEEEECCHHHCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHH
HMVSHYYAKEPMDQITRGLFALASYNAGPDRIERLRSVAAAEGYDPNLWFNNVEFIAARR
HHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
IGAETTNYVSNVYKYYLAYKMTIEQDARRQASRSGALPGKTR
CCCHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA