Click here to switch to the map view.

The map label for this gene is pspA [C]

Identifier: 116621502

GI number: 116621502

Start: 3037380

End: 3038156

Strand: Reverse

Name: pspA [C]

Synonym: Acid_2384

Alternate gene names: 116621502

Gene position: 3038156-3037380 (Counterclockwise)

Preceding gene: 116621503

Following gene: 116621501

Centisome position: 30.49

GC content: 63.84

Gene sequence:

>777_bases
ATGGCATTTCTTTCGGACATTTTCGGGCGTCTCGGCCGCGTGGCCCGCGGCCAGGCAAACGCCGGCGTCGATACCATCGA
AGACGCGACTTTTGAGGCCACCGTGCGGCAGACCGTCGCCGACATGAAGACCGAGTTGAACAACGTGGTGCGCGCGTCGG
CGCAGGCGATGAGCAACTACAACAGCCTGGACGCGGAGTATCAGAAGTACGTGCGCCAGTCGCAGGAATGGAAGGACCGC
GCGGGAATGGCGCTCGACGCGGGCAATGAAGACCTGGCCAAGAAGGCGCTGGCCAAGAAGGCCGAGTGCGACATCCAGGT
CAACTCCATGAAGACCGCCGTGGACAATGCCTCCGCCACAAGCGAAAAGCTGAAGGCGCAGGTGGGCGAGCTCAAGCGCA
AAATCGAAGAAGGCGAGCGCACGGCCGGCACGCTGGTGGCGCGCAAGAATGCCGCGGTGGCGCAGCGCAAGGTGAGTGAA
GCCATGGCTGGAGTGGGCAGCGCCGATAACGCGTTCGCCGCGCTCAACCGCTACGAACAGACTGTGGCGAAGGAAGAAGC
CACGGCCAAGGCCTTCGACCAGTTGGCGAGCGTCGGCAAAGATGACAACCTGGAGAAGGAATTCGCGCAGCTCGGCGGAC
ACAGCGTGGATGCCGAACTCGCCGCGCTCAAAGCCGAGCGGCAGAAACCCTCGGCGCCCTATATCGCCAAGGAACTGCCG
GGGCCGGCCGGTTCGCTTTCCACCGGCACGCAGATCGGGGAAAAGACCGGGGCGTAA

Upstream 100 bases:

>100_bases
ATTCTTTCGTGCGTGCATTTTCTGCTGGTGGATGTAATGAGCGCGCGGACCCTGTTGAGCAGCCTGGCGGCGTAAGAGGA
GAATAAGAGGAGAGACGATT

Downstream 100 bases:

>100_bases
CCGGGCGCTTCAATGATCACGTTCATTGTTGTAGCCATTCTCGGAGCGCTGCTGGCGATCGTGCTTATGAACCTATTCAA
GAAAGATCCGAAGCCGCTTC

Product: phage shock protein PspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAFLSDIFGRLGRVARGQANAGVDTIEDATFEATVRQTVADMKTELNNVVRASAQAMSNYNSLDAEYQKYVRQSQEWKDR
AGMALDAGNEDLAKKALAKKAECDIQVNSMKTAVDNASATSEKLKAQVGELKRKIEEGERTAGTLVARKNAAVAQRKVSE
AMAGVGSADNAFAALNRYEQTVAKEEATAKAFDQLASVGKDDNLEKEFAQLGGHSVDAELAALKAERQKPSAPYIAKELP
GPAGSLSTGTQIGEKTGA

Sequences:

>Translated_258_residues
MAFLSDIFGRLGRVARGQANAGVDTIEDATFEATVRQTVADMKTELNNVVRASAQAMSNYNSLDAEYQKYVRQSQEWKDR
AGMALDAGNEDLAKKALAKKAECDIQVNSMKTAVDNASATSEKLKAQVGELKRKIEEGERTAGTLVARKNAAVAQRKVSE
AMAGVGSADNAFAALNRYEQTVAKEEATAKAFDQLASVGKDDNLEKEFAQLGGHSVDAELAALKAERQKPSAPYIAKELP
GPAGSLSTGTQIGEKTGA
>Mature_257_residues
AFLSDIFGRLGRVARGQANAGVDTIEDATFEATVRQTVADMKTELNNVVRASAQAMSNYNSLDAEYQKYVRQSQEWKDRA
GMALDAGNEDLAKKALAKKAECDIQVNSMKTAVDNASATSEKLKAQVGELKRKIEEGERTAGTLVARKNAAVAQRKVSEA
MAGVGSADNAFAALNRYEQTVAKEEATAKAFDQLASVGKDDNLEKEFAQLGGHSVDAELAALKAERQKPSAPYIAKELPG
PAGSLSTGTQIGEKTGA

Specific function: The Phage Shock Protein (Psp) Operon (Pspabce) May Play A Significant Role In The Competition For Survival Under Nutrient- Or Energy-Limited Conditions. Pspa Has A Role As A Negative Regulator, Is Required For Efficient Translocation And Has An Unknown Fu

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=220, Percent_Identity=28.6363636363636, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 27447; Mature: 27316

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLSDIFGRLGRVARGQANAGVDTIEDATFEATVRQTVADMKTELNNVVRASAQAMSNY
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NSLDAEYQKYVRQSQEWKDRAGMALDAGNEDLAKKALAKKAECDIQVNSMKTAVDNASAT
CCCCHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCEEHHHHHHHHHCCHHH
SEKLKAQVGELKRKIEEGERTAGTLVARKNAAVAQRKVSEAMAGVGSADNAFAALNRYEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TVAKEEATAKAFDQLASVGKDDNLEKEFAQLGGHSVDAELAALKAERQKPSAPYIAKELP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHCC
GPAGSLSTGTQIGEKTGA
CCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
AFLSDIFGRLGRVARGQANAGVDTIEDATFEATVRQTVADMKTELNNVVRASAQAMSNY
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NSLDAEYQKYVRQSQEWKDRAGMALDAGNEDLAKKALAKKAECDIQVNSMKTAVDNASAT
CCCCHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCEEHHHHHHHHHCCHHH
SEKLKAQVGELKRKIEEGERTAGTLVARKNAAVAQRKVSEAMAGVGSADNAFAALNRYEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TVAKEEATAKAFDQLASVGKDDNLEKEFAQLGGHSVDAELAALKAERQKPSAPYIAKELP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHCC
GPAGSLSTGTQIGEKTGA
CCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]