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The map label for this gene is vapI [H]

Identifier: 116621448

GI number: 116621448

Start: 2974549

End: 2974878

Strand: Reverse

Name: vapI [H]

Synonym: Acid_2330

Alternate gene names: 116621448

Gene position: 2974878-2974549 (Counterclockwise)

Preceding gene: 116621449

Following gene: 116621447

Centisome position: 29.85

GC content: 55.45

Gene sequence:

>330_bases
ATGAGCATAATGCGTCCTGAAGGCGGCTGGAACATTGCCCGCCCTCCGGTTCACCCCGGCGAAATGCTTCGTGAGGAGTT
CATGAAACCGTTGGGCATTTCTATCAATGGACTAGCTCTGGAACTTCACGTACCGGTCACCCGCATCAGTCAGATCGTCA
ACGAGCGTCGCGGGATTACCGCCGACACAGCATTGCGCCTGGCGCAGCACTTCGGCACGAGCGCCGATTTCTGGATGAAC
ATTCAGAAGGATTACGAGTTGCTCTTGACCAGGCAAAAGTCATTGAAATCCATCGAGCAGCAGGTACGGAGACGAACGGT
CGCCGCATGA

Upstream 100 bases:

>100_bases
CAGCGCTCAATGGCGCATCTGTTTTCGTTGGGAAAGCGACGGCGTTTACGATGTCGAGATTGTGGATTACCACTAACCAC
TAAGAGGAGGGACAGAGATT

Downstream 100 bases:

>100_bases
TTTCGCGGATACGAGTTTGTCACTGCAAAGGTAAGCGGAAGCTTGCCCAAAGGGCGTCGAGACGGCCAGGCACGGAGCCA
AAAGATAAACTAGATTTGGA

Product: XRE family plasmid maintenance system antidote protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 109; Mature: 108

Protein sequence:

>109_residues
MSIMRPEGGWNIARPPVHPGEMLREEFMKPLGISINGLALELHVPVTRISQIVNERRGITADTALRLAQHFGTSADFWMN
IQKDYELLLTRQKSLKSIEQQVRRRTVAA

Sequences:

>Translated_109_residues
MSIMRPEGGWNIARPPVHPGEMLREEFMKPLGISINGLALELHVPVTRISQIVNERRGITADTALRLAQHFGTSADFWMN
IQKDYELLLTRQKSLKSIEQQVRRRTVAA
>Mature_108_residues
SIMRPEGGWNIARPPVHPGEMLREEFMKPLGISINGLALELHVPVTRISQIVNERRGITADTALRLAQHFGTSADFWMNI
QKDYELLLTRQKSLKSIEQQVRRRTVAA

Specific function: Unknown

COG id: COG3093

COG function: function code R; Plasmid maintenance system antidote protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081745, Length=95, Percent_Identity=37.8947368421053, Blast_Score=71, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013430
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 12435; Mature: 12303

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIMRPEGGWNIARPPVHPGEMLREEFMKPLGISINGLALELHVPVTRISQIVNERRGIT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHCCCC
ADTALRLAQHFGTSADFWMNIQKDYELLLTRQKSLKSIEQQVRRRTVAA
HHHHHHHHHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIMRPEGGWNIARPPVHPGEMLREEFMKPLGISINGLALELHVPVTRISQIVNERRGIT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHCCCC
ADTALRLAQHFGTSADFWMNIQKDYELLLTRQKSLKSIEQQVRRRTVAA
HHHHHHHHHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8169216 [H]