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The map label for this gene is ilvB [H]

Identifier: 116621362

GI number: 116621362

Start: 2867220

End: 2869046

Strand: Reverse

Name: ilvB [H]

Synonym: Acid_2243

Alternate gene names: 116621362

Gene position: 2869046-2867220 (Counterclockwise)

Preceding gene: 116621363

Following gene: 116621361

Centisome position: 28.79

GC content: 61.41

Gene sequence:

>1827_bases
ATGATTAAGCTTTCCGACTACGTTATGGGTTTTGTGGCGAACAGCGGCGTCAAGCACGTCTTCCTGGTCGTCGGCGGCGG
AGCCATGCACCTCAACGATTCTCTCTCGAGGCGCAAGGAAATCGAGTTTGTCTGCAACCTCCACGAACAGGCCAGTGCGA
TCGCGGCGGAGAACTATTCCAAATCCACCAATCACCTCGGCGTCGCCCTGGTAACCACCGGACCCGGCGGCACCAATGCG
GTGACCGGCGTGGCCGGCGCCTGGCTGGATTCCACGCCGTGCCTGTTCATTTCGGGCCAGGTGAAACGGGCCGACAGCAT
GTTCCGCCCGGATGGCACACCGCTCGGCGTGCGGCAGGTGGGCGTCCAGGAAGTAGATATCGTTTCCATCGTTCGCCCCA
TCACCAAGTACGCCGTGACTGTGAACGACCCCCAATCCATTCGCTATCACCTGGAACGGGCCCTATACCTGGCGCAGAGC
GGCCGTCCCGGACCGGTGTGGATCGATATTCCGCTCGACGTTCAAGCCGCCCCCATCGACGAAGAAAACCTCCCCGCCTT
CGAAATACCCGCGCCGGAATCCCACCATGAGACTCTGCAAGCGCAGGTCCGCGAAATTATCGATGCGTTGAATCGCGCCG
AACGTCCCATGCTGCTGGCCGGCAACGGGATCCGGCTGGCCAGGGCCGAGAAGGAATTCCTGGAACTCGTCGAATTGCTC
GATATCCCGCTCGAAACCACGTGGCTGGCCATCGATCTCATCGGCGACGATCACCCGCTGTTCGTGGGGCGGCCGGGCAC
GATCGCTCCCCGCGGCGCCAATTTCGCCATCCAGAATACCGATTTTCTGCTTGCCATCGGCGCCCGCCTCGATCGCGTCA
TAACCGGCTTTGCGCCGGAGAAATTCGCGCCCTCTGCCTGGAAGGCAATGGTCGATATCGATCCCGCCGAACTTCAAAAG
ATGGGGAATACGGTTTGCGTCAAAGTCCGCGCCGATGCCGGAGCGTTCATGCGCGAGATGCTGAGTCAGGCCCGCGCGAT
ATCGCCGCGGGATCGATCGAGCTGGAAACAGCGCTGCGCCGATTGGAAGCGTCGATACCCTCTTGTGCTTGAGGAGCATC
GCAAACCGGAAGGACCCGTCAGCTGCTACCACCTGGCCGATGTCCTTTCGGACGAACTCGGCCCCGACGCCCATTATGTT
TCCGGGAGTTCCGGCAGCGGCATCGAGCTCGTGCTGCTCGCCATGCGCGTGAAGCAGGGGCAGAGGATTTATCACACCAC
CGCGCTCGGCGCCATGGGTTACGGCATTGCCGCATCGATTGGCGTGTGTATGGCCTCCGGCCGCCGTGAAGTAGTCTGCG
TCGATGGCGATGGCGGCTTCCAGTTCAATATTCAAGAGTTGGAGACGGTAGCCCGTCTCCAGCTTCCCATCAAGTTTTTC
GTTCTCAACAACAACGGTTACTCTTCGATCCGCGCCTCCCAGAAGGCATTTTTCGGGGAAGCCACCATCGGCTGCGACAC
CAGCACCGGCCAGACACTGCCGGACCTTCGCAAAGTCGCAACAGCCTACGGCATTCAGACCGACGTCATCATGGACCAGA
CCAACCTGCGCGCGGAAATCAGGCGCGTGCTCGCTAGACCGGGACCCGTTGTCTGCGATGTCCACGTCGTTCCCGATGAG
GTCCGGATGCCGCGCCTATCCTCCGTCCAGCTGCCGGACGGTTCGTTCGTGTCCAAGCCGCTGGAGGATCTCTGGCCCTT
TCTCGATCGCGCCGAATTTGAGGCCAACATGCTACGGCCGACTGCCGGGGAGCCCGTCGCTTCGTGA

Upstream 100 bases:

>100_bases
CACATAGAAGATACGATGCTTTGTGTTTGGTTCAATGATCCCTTGAAATCCATTGGACCTCGTCTGTTGAACCTCCAATG
AAGCTCCTATTAACCAGATC

Downstream 100 bases:

>100_bases
TCCGCATCGGACAAATGCAAGGACGCCTGTCCCCTCCGGAAGGCGGACGAATTCAGTTTTTCCCGCGCCTGCGCTGGCAG
GACGAATTTGCCCTCGCGGC

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MIKLSDYVMGFVANSGVKHVFLVVGGGAMHLNDSLSRRKEIEFVCNLHEQASAIAAENYSKSTNHLGVALVTTGPGGTNA
VTGVAGAWLDSTPCLFISGQVKRADSMFRPDGTPLGVRQVGVQEVDIVSIVRPITKYAVTVNDPQSIRYHLERALYLAQS
GRPGPVWIDIPLDVQAAPIDEENLPAFEIPAPESHHETLQAQVREIIDALNRAERPMLLAGNGIRLARAEKEFLELVELL
DIPLETTWLAIDLIGDDHPLFVGRPGTIAPRGANFAIQNTDFLLAIGARLDRVITGFAPEKFAPSAWKAMVDIDPAELQK
MGNTVCVKVRADAGAFMREMLSQARAISPRDRSSWKQRCADWKRRYPLVLEEHRKPEGPVSCYHLADVLSDELGPDAHYV
SGSSGSGIELVLLAMRVKQGQRIYHTTALGAMGYGIAASIGVCMASGRREVVCVDGDGGFQFNIQELETVARLQLPIKFF
VLNNNGYSSIRASQKAFFGEATIGCDTSTGQTLPDLRKVATAYGIQTDVIMDQTNLRAEIRRVLARPGPVVCDVHVVPDE
VRMPRLSSVQLPDGSFVSKPLEDLWPFLDRAEFEANMLRPTAGEPVAS

Sequences:

>Translated_608_residues
MIKLSDYVMGFVANSGVKHVFLVVGGGAMHLNDSLSRRKEIEFVCNLHEQASAIAAENYSKSTNHLGVALVTTGPGGTNA
VTGVAGAWLDSTPCLFISGQVKRADSMFRPDGTPLGVRQVGVQEVDIVSIVRPITKYAVTVNDPQSIRYHLERALYLAQS
GRPGPVWIDIPLDVQAAPIDEENLPAFEIPAPESHHETLQAQVREIIDALNRAERPMLLAGNGIRLARAEKEFLELVELL
DIPLETTWLAIDLIGDDHPLFVGRPGTIAPRGANFAIQNTDFLLAIGARLDRVITGFAPEKFAPSAWKAMVDIDPAELQK
MGNTVCVKVRADAGAFMREMLSQARAISPRDRSSWKQRCADWKRRYPLVLEEHRKPEGPVSCYHLADVLSDELGPDAHYV
SGSSGSGIELVLLAMRVKQGQRIYHTTALGAMGYGIAASIGVCMASGRREVVCVDGDGGFQFNIQELETVARLQLPIKFF
VLNNNGYSSIRASQKAFFGEATIGCDTSTGQTLPDLRKVATAYGIQTDVIMDQTNLRAEIRRVLARPGPVVCDVHVVPDE
VRMPRLSSVQLPDGSFVSKPLEDLWPFLDRAEFEANMLRPTAGEPVAS
>Mature_608_residues
MIKLSDYVMGFVANSGVKHVFLVVGGGAMHLNDSLSRRKEIEFVCNLHEQASAIAAENYSKSTNHLGVALVTTGPGGTNA
VTGVAGAWLDSTPCLFISGQVKRADSMFRPDGTPLGVRQVGVQEVDIVSIVRPITKYAVTVNDPQSIRYHLERALYLAQS
GRPGPVWIDIPLDVQAAPIDEENLPAFEIPAPESHHETLQAQVREIIDALNRAERPMLLAGNGIRLARAEKEFLELVELL
DIPLETTWLAIDLIGDDHPLFVGRPGTIAPRGANFAIQNTDFLLAIGARLDRVITGFAPEKFAPSAWKAMVDIDPAELQK
MGNTVCVKVRADAGAFMREMLSQARAISPRDRSSWKQRCADWKRRYPLVLEEHRKPEGPVSCYHLADVLSDELGPDAHYV
SGSSGSGIELVLLAMRVKQGQRIYHTTALGAMGYGIAASIGVCMASGRREVVCVDGDGGFQFNIQELETVARLQLPIKFF
VLNNNGYSSIRASQKAFFGEATIGCDTSTGQTLPDLRKVATAYGIQTDVIMDQTNLRAEIRRVLARPGPVVCDVHVVPDE
VRMPRLSSVQLPDGSFVSKPLEDLWPFLDRAEFEANMLRPTAGEPVAS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=25.4867256637168, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI21361361, Length=560, Percent_Identity=25.7142857142857, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=30.3571428571429, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI87081685, Length=539, Percent_Identity=32.0964749536178, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1786717, Length=501, Percent_Identity=29.7405189620758, Blast_Score=202, Evalue=6e-53,
Organism=Escherichia coli, GI1788716, Length=458, Percent_Identity=25.9825327510917, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787096, Length=567, Percent_Identity=22.7513227513227, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17531299, Length=565, Percent_Identity=23.716814159292, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=23.716814159292, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17542570, Length=538, Percent_Identity=23.6059479553903, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323755, Length=565, Percent_Identity=28.8495575221239, Blast_Score=212, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=22.0858895705521, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19922626, Length=490, Percent_Identity=26.734693877551, Blast_Score=157, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 66339; Mature: 66339

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLSDYVMGFVANSGVKHVFLVVGGGAMHLNDSLSRRKEIEFVCNLHEQASAIAAENYS
CEEEHHHHHHHHHCCCCCEEEEEEECCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHCCC
KSTNHLGVALVTTGPGGTNAVTGVAGAWLDSTPCLFISGQVKRADSMFRPDGTPLGVRQV
CCCCCEEEEEEEECCCCCCCHHHCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCHHHC
GVQEVDIVSIVRPITKYAVTVNDPQSIRYHLERALYLAQSGRPGPVWIDIPLDVQAAPID
CCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCCC
EENLPAFEIPAPESHHETLQAQVREIIDALNRAERPMLLAGNGIRLARAEKEFLELVELL
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEHHHHHHHHHHHHH
DIPLETTWLAIDLIGDDHPLFVGRPGTIAPRGANFAIQNTDFLLAIGARLDRVITGFAPE
CCCCCCEEEEEEEECCCCCEEEECCCCCCCCCCCEEEECCCEEEEEHHHHHHHHHCCCCC
KFAPSAWKAMVDIDPAELQKMGNTVCVKVRADAGAFMREMLSQARAISPRDRSSWKQRCA
CCCCCHHHEEEECCHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
DWKRRYPLVLEEHRKPEGPVSCYHLADVLSDELGPDAHYVSGSSGSGIELVLLAMRVKQG
HHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCC
QRIYHTTALGAMGYGIAASIGVCMASGRREVVCVDGDGGFQFNIQELETVARLQLPIKFF
CEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEHHHHHHHHHEECCEEEE
VLNNNGYSSIRASQKAFFGEATIGCDTSTGQTLPDLRKVATAYGIQTDVIMDQTNLRAEI
EECCCCCCHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHH
RRVLARPGPVVCDVHVVPDEVRMPRLSSVQLPDGSFVSKPLEDLWPFLDRAEFEANMLRP
HHHHCCCCCEEEEEEECCCCCCCCCCCEEECCCCCHHHCCHHHHHHHHHHHHCHHHCCCC
TAGEPVAS
CCCCCCCC
>Mature Secondary Structure
MIKLSDYVMGFVANSGVKHVFLVVGGGAMHLNDSLSRRKEIEFVCNLHEQASAIAAENYS
CEEEHHHHHHHHHCCCCCEEEEEEECCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHCCC
KSTNHLGVALVTTGPGGTNAVTGVAGAWLDSTPCLFISGQVKRADSMFRPDGTPLGVRQV
CCCCCEEEEEEEECCCCCCCHHHCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCHHHC
GVQEVDIVSIVRPITKYAVTVNDPQSIRYHLERALYLAQSGRPGPVWIDIPLDVQAAPID
CCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCCC
EENLPAFEIPAPESHHETLQAQVREIIDALNRAERPMLLAGNGIRLARAEKEFLELVELL
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEHHHHHHHHHHHHH
DIPLETTWLAIDLIGDDHPLFVGRPGTIAPRGANFAIQNTDFLLAIGARLDRVITGFAPE
CCCCCCEEEEEEEECCCCCEEEECCCCCCCCCCCEEEECCCEEEEEHHHHHHHHHCCCCC
KFAPSAWKAMVDIDPAELQKMGNTVCVKVRADAGAFMREMLSQARAISPRDRSSWKQRCA
CCCCCHHHEEEECCHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
DWKRRYPLVLEEHRKPEGPVSCYHLADVLSDELGPDAHYVSGSSGSGIELVLLAMRVKQG
HHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCC
QRIYHTTALGAMGYGIAASIGVCMASGRREVVCVDGDGGFQFNIQELETVARLQLPIKFF
CEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEHHHHHHHHHEECCEEEE
VLNNNGYSSIRASQKAFFGEATIGCDTSTGQTLPDLRKVATAYGIQTDVIMDQTNLRAEI
EECCCCCCHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCHHHHHH
RRVLARPGPVVCDVHVVPDEVRMPRLSSVQLPDGSFVSKPLEDLWPFLDRAEFEANMLRP
HHHHCCCCCEEEEEEECCCCCCCCCCCEEECCCCCHHHCCHHHHHHHHHHHHCHHHCCCC
TAGEPVAS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]