Click here to switch to the map view.

The map label for this gene is sigW [H]

Identifier: 116621233

GI number: 116621233

Start: 2703684

End: 2704283

Strand: Reverse

Name: sigW [H]

Synonym: Acid_2114

Alternate gene names: 116621233

Gene position: 2704283-2703684 (Counterclockwise)

Preceding gene: 116621238

Following gene: 116621232

Centisome position: 27.14

GC content: 63.67

Gene sequence:

>600_bases
ATGCAAAATCTTACGGACGGCGAATTGGTGCGGCTGGCTCAGCAAGGCAGCGAGCGGGCCTTTGGGGAGCTGATGAAGCG
CCATGCACGCGGAATGCACCAGATCGCCTATTCGATTCTGCGGGACCAGGAAGAGGCGGCGGACGAATTGCAAAACGCGT
GGTGGAAGGTCTGGCAGAACCTCGACAGGTTCGCGGGCGAATCGCGATTCAGCACCTGGATGACGCGCATTGTAATCAAT
CAGTGCCTGATGCGGCTGCGCCAATCCCGGCGGAGGCGCTTTCTCTATATGGATGCGCCGGCGCCGGGCGAAGCCAGCGT
GTGCTTCGAGCTGCGAGACCAGTCCAGTTCGCCGGAGGAGGACGTTGCGCGGCGCGAGGCGACCGAGGTAGTGTGCCGGG
AGATCGGGCGGATTCCACCGCTTCTGCGCCAAGCGCTGGTGCTGCGCGAAGTAATGGAGTTGCCCATGCCACAAGTGGCC
GAGGAGTTGGGTATCTCGCTGGCGGCCGCAAAATCGCGGCTCTTGCGGGCGAAGCACGAGCTGCGCGTACGCTTGGAGAA
GTACTGCGGCGGGATGGGTCCGGCCGGCGTGACGGCGTGA

Upstream 100 bases:

>100_bases
GCTCTACTAAATTCATCGCTGCTCATTATTTAAAATTCCGGAACTTCGTTGGTAGGCTGGCCATAGCGACACGAATTGGA
CTCAGGAGGAGTGGGGGAAA

Downstream 100 bases:

>100_bases
GCTTCTGGTACAATGAGGGTCTGCCCTGTGGCGCTATCGTCTAACGGTTAGGACAGAGCCCTCTCAAGGCTTAAATACGG
GTTCGATTCCCGTTAGCGCT

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MQNLTDGELVRLAQQGSERAFGELMKRHARGMHQIAYSILRDQEEAADELQNAWWKVWQNLDRFAGESRFSTWMTRIVIN
QCLMRLRQSRRRRFLYMDAPAPGEASVCFELRDQSSSPEEDVARREATEVVCREIGRIPPLLRQALVLREVMELPMPQVA
EELGISLAAAKSRLLRAKHELRVRLEKYCGGMGPAGVTA

Sequences:

>Translated_199_residues
MQNLTDGELVRLAQQGSERAFGELMKRHARGMHQIAYSILRDQEEAADELQNAWWKVWQNLDRFAGESRFSTWMTRIVIN
QCLMRLRQSRRRRFLYMDAPAPGEASVCFELRDQSSSPEEDVARREATEVVCREIGRIPPLLRQALVLREVMELPMPQVA
EELGISLAAAKSRLLRAKHELRVRLEKYCGGMGPAGVTA
>Mature_199_residues
MQNLTDGELVRLAQQGSERAFGELMKRHARGMHQIAYSILRDQEEAADELQNAWWKVWQNLDRFAGESRFSTWMTRIVIN
QCLMRLRQSRRRRFLYMDAPAPGEASVCFELRDQSSSPEEDVARREATEVVCREIGRIPPLLRQALVLREVMELPMPQVA
EELGISLAAAKSRLLRAKHELRVRLEKYCGGMGPAGVTA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=183, Percent_Identity=30.6010928961749, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22810; Mature: 22810

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLTDGELVRLAQQGSERAFGELMKRHARGMHQIAYSILRDQEEAADELQNAWWKVWQN
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRFAGESRFSTWMTRIVINQCLMRLRQSRRRRFLYMDAPAPGEASVCFELRDQSSSPEE
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCCCCCHH
DVARREATEVVCREIGRIPPLLRQALVLREVMELPMPQVAEELGISLAAAKSRLLRAKHE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHH
LRVRLEKYCGGMGPAGVTA
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQNLTDGELVRLAQQGSERAFGELMKRHARGMHQIAYSILRDQEEAADELQNAWWKVWQN
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRFAGESRFSTWMTRIVINQCLMRLRQSRRRRFLYMDAPAPGEASVCFELRDQSSSPEE
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCCCCCHH
DVARREATEVVCREIGRIPPLLRQALVLREVMELPMPQVAEELGISLAAAKSRLLRAKHE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHH
LRVRLEKYCGGMGPAGVTA
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]