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The map label for this gene is lipB [H]

Identifier: 116620959

GI number: 116620959

Start: 2293125

End: 2293910

Strand: Reverse

Name: lipB [H]

Synonym: Acid_1840

Alternate gene names: 116620959

Gene position: 2293910-2293125 (Counterclockwise)

Preceding gene: 116620960

Following gene: 116620958

Centisome position: 23.02

GC content: 63.23

Gene sequence:

>786_bases
ATGCCATCGCCCCCGTGTAAGATCACTGTCCTCGGCTCCGGCACCAGCGTCGGGGTTCCGACGATCGGCTGCCATTGCGA
CGTGTGTACGTCGCAGGATGCGCGCGATAAGCGGCTGCGGCCTTCCGTCCTGGTCAGCTTTGACGATCGCAACGTGCTGA
TCGATACCACGCCCGACCTGAGGACGCAGGCGCTGCGGGCGCGCATCGAGCGGCTCGACGCCGTGCTCTTCACCCATTCC
CACGCCGATCACCTGATGGGGCTGGACGATGTGCGGCCCTTCAATTTCCGGCAGAAGGGACAGATTCCGATCTTCGCGTC
GCCGGAGACGATGGCGGCGGTCCAACGCTGTTTCCAGTACATATTCGATAACGGCAAGAAGGAATCGAACGTGCCCAAGC
TGGACGCGCGCGTGCTTGATGGAACTCCGTTCGACGTGTTCGGCATGGAATTCACGCCGATTCCGATTCTGCACGGTTCG
CAGACCATCTACGGATTTCGTTTCGGCGGCGCGGCCTATCTGACCGATCACAGCGACATCCCGGAATCGTCAATGGAGAT
GCTGAAGGGCCTCGATGTGCTCTTCCTGGACGCGCTGCGCTACAAACCGCACCCCACGCACTCCACCGTGGAGCGCTCCA
TCCGCACGGTGGACCAGCTGGCGCCGCACCGGGCCTTCTTCACGCATATCTGTCACGACCTGGGGCACGAGCGCGCGGAG
AGCATGCTGCCGCCGCACATCCGGCTGGCGTACGACGGCCTGGAAATCCAGGTGGGAGGCCGCTAG

Upstream 100 bases:

>100_bases
GGAAGAGCAGCGCATGCTGGAGAATTCCGTGACCGAGCTGCGCTTCCGGTACGTGCAGATCTCCGACGAGGTGGCGAAGG
CAGCGGCGCAGAAAGCCTGA

Downstream 100 bases:

>100_bases
GCGATGCAGGTTTACCGCAGCCTGGCGGAGATTCCGCGCGATTTCGGACCGAGCGCGGTAACCATCGGGAACTTCGACGG
CGTACATTTCGGGCACCGTC

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MPSPPCKITVLGSGTSVGVPTIGCHCDVCTSQDARDKRLRPSVLVSFDDRNVLIDTTPDLRTQALRARIERLDAVLFTHS
HADHLMGLDDVRPFNFRQKGQIPIFASPETMAAVQRCFQYIFDNGKKESNVPKLDARVLDGTPFDVFGMEFTPIPILHGS
QTIYGFRFGGAAYLTDHSDIPESSMEMLKGLDVLFLDALRYKPHPTHSTVERSIRTVDQLAPHRAFFTHICHDLGHERAE
SMLPPHIRLAYDGLEIQVGGR

Sequences:

>Translated_261_residues
MPSPPCKITVLGSGTSVGVPTIGCHCDVCTSQDARDKRLRPSVLVSFDDRNVLIDTTPDLRTQALRARIERLDAVLFTHS
HADHLMGLDDVRPFNFRQKGQIPIFASPETMAAVQRCFQYIFDNGKKESNVPKLDARVLDGTPFDVFGMEFTPIPILHGS
QTIYGFRFGGAAYLTDHSDIPESSMEMLKGLDVLFLDALRYKPHPTHSTVERSIRTVDQLAPHRAFFTHICHDLGHERAE
SMLPPHIRLAYDGLEIQVGGR
>Mature_260_residues
PSPPCKITVLGSGTSVGVPTIGCHCDVCTSQDARDKRLRPSVLVSFDDRNVLIDTTPDLRTQALRARIERLDAVLFTHSH
ADHLMGLDDVRPFNFRQKGQIPIFASPETMAAVQRCFQYIFDNGKKESNVPKLDARVLDGTPFDVFGMEFTPIPILHGSQ
TIYGFRFGGAAYLTDHSDIPESSMEMLKGLDVLFLDALRYKPHPTHSTVERSIRTVDQLAPHRAFFTHICHDLGHERAES
MLPPHIRLAYDGLEIQVGGR

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI1790530, Length=258, Percent_Identity=28.6821705426357, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 29164; Mature: 29032

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPPCKITVLGSGTSVGVPTIGCHCDVCTSQDARDKRLRPSVLVSFDDRNVLIDTTPDL
CCCCCEEEEEEECCCCCCCCEECCEEECCCCCCCHHHCCCCEEEEEECCCEEEEECCCCH
RTQALRARIERLDAVLFTHSHADHLMGLDDVRPFNFRQKGQIPIFASPETMAAVQRCFQY
HHHHHHHHHHHHHHHEEECCCHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
IFDNGKKESNVPKLDARVLDGTPFDVFGMEFTPIPILHGSQTIYGFRFGGAAYLTDHSDI
HHCCCCCCCCCCCCCEEEECCCCCEEECCCCCCCEEECCCCEEEEEEECCEEEECCCCCC
PESSMEMLKGLDVLFLDALRYKPHPTHSTVERSIRTVDQLAPHRAFFTHICHDLGHERAE
CHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SMLPPHIRLAYDGLEIQVGGR
HCCCCCEEEEECCEEEEECCC
>Mature Secondary Structure 
PSPPCKITVLGSGTSVGVPTIGCHCDVCTSQDARDKRLRPSVLVSFDDRNVLIDTTPDL
CCCCEEEEEEECCCCCCCCEECCEEECCCCCCCHHHCCCCEEEEEECCCEEEEECCCCH
RTQALRARIERLDAVLFTHSHADHLMGLDDVRPFNFRQKGQIPIFASPETMAAVQRCFQY
HHHHHHHHHHHHHHHEEECCCHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
IFDNGKKESNVPKLDARVLDGTPFDVFGMEFTPIPILHGSQTIYGFRFGGAAYLTDHSDI
HHCCCCCCCCCCCCCEEEECCCCCEEECCCCCCCEEECCCCEEEEEEECCEEEECCCCCC
PESSMEMLKGLDVLFLDALRYKPHPTHSTVERSIRTVDQLAPHRAFFTHICHDLGHERAE
CHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SMLPPHIRLAYDGLEIQVGGR
HCCCCCEEEEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]