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The map label for this gene is trmH [C]

Identifier: 116620879

GI number: 116620879

Start: 2188204

End: 2188746

Strand: Direct

Name: trmH [C]

Synonym: Acid_1760

Alternate gene names: 116620879

Gene position: 2188204-2188746 (Clockwise)

Preceding gene: 116620877

Following gene: 116620880

Centisome position: 21.96

GC content: 64.83

Gene sequence:

>543_bases
ATGGAACGTATCCCTTTTCAGGCGACGGCAGCGGGAGCGCTGTACGAGGACGTGGGGCCGCTGCCGGCGGCGGTGCTGCT
GGATAACGTGCGCAGCATGTACAATGTCGGATCGTTTTTCCGCACGGCGGACGCGGCCAGCGTGGAGAAGCTCTATCTCT
CCGGGATCACCGCCCAACCCCCGAAGAGCGCGATCACGAAGACGGCGCTCGGGGCGGAGAACACGGTGTGCTGGGAGCAC
GCGTGGGAGGCCCGGCCGCTGGTGGAGAAGGCGCGCGCGCAAGGGTATGAGATCGCGGCGGTCGAGACCAGCGTCCACGC
CGTGGATCTATTCGATTGGACGCCGCGCTTCCCGGTGCTGGTGATTTTCGGGCACGAGGTGGATGGCATCCGGCCGGCGA
TTTCGGCGCTCTGCGATACGCACGTGCGCATCCCGATGTTGGGCGCAAAGCATTCGTTGAACGTGGCGACGGCCGGCGGT
GTCGTAATTTACGAACTGCTGCGCAAGTACCGGGTGTTGAGCGCTACGATGAGACGTGCCTGA

Upstream 100 bases:

>100_bases
AGGATGGACGGCTCCTCGTTGTGGATCGGGATCACGATGGACAGCATTGCCATGTAAACTTTCAGTTTAGCATCGTGTGG
TGCTATACTTTCGCGCTCTG

Downstream 100 bases:

>100_bases
ACCGCTGCCGCGCATTCGCATGAACCTGGATTTCATGCCCTCGCCTTCGCCCGAACATCCCGGGCTATTCATCCGCGACC
CCTACAAATTTTCGGACGCG

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MERIPFQATAAGALYEDVGPLPAAVLLDNVRSMYNVGSFFRTADAASVEKLYLSGITAQPPKSAITKTALGAENTVCWEH
AWEARPLVEKARAQGYEIAAVETSVHAVDLFDWTPRFPVLVIFGHEVDGIRPAISALCDTHVRIPMLGAKHSLNVATAGG
VVIYELLRKYRVLSATMRRA

Sequences:

>Translated_180_residues
MERIPFQATAAGALYEDVGPLPAAVLLDNVRSMYNVGSFFRTADAASVEKLYLSGITAQPPKSAITKTALGAENTVCWEH
AWEARPLVEKARAQGYEIAAVETSVHAVDLFDWTPRFPVLVIFGHEVDGIRPAISALCDTHVRIPMLGAKHSLNVATAGG
VVIYELLRKYRVLSATMRRA
>Mature_180_residues
MERIPFQATAAGALYEDVGPLPAAVLLDNVRSMYNVGSFFRTADAASVEKLYLSGITAQPPKSAITKTALGAENTVCWEH
AWEARPLVEKARAQGYEIAAVETSVHAVDLFDWTPRFPVLVIFGHEVDGIRPAISALCDTHVRIPMLGAKHSLNVATAGG
VVIYELLRKYRVLSATMRRA

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=148, Percent_Identity=31.0810810810811, Blast_Score=68, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21355819, Length=144, Percent_Identity=31.9444444444444, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 19593; Mature: 19593

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIPFQATAAGALYEDVGPLPAAVLLDNVRSMYNVGSFFRTADAASVEKLYLSGITAQP
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
PKSAITKTALGAENTVCWEHAWEARPLVEKARAQGYEIAAVETSVHAVDLFDWTPRFPVL
CHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHCCEEEEEEECCEEEEEECCCCCCCCEE
VIFGHEVDGIRPAISALCDTHVRIPMLGAKHSLNVATAGGVVIYELLRKYRVLSATMRRA
EEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERIPFQATAAGALYEDVGPLPAAVLLDNVRSMYNVGSFFRTADAASVEKLYLSGITAQP
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
PKSAITKTALGAENTVCWEHAWEARPLVEKARAQGYEIAAVETSVHAVDLFDWTPRFPVL
CHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHCCEEEEEEECCEEEEEECCCCCCCCEE
VIFGHEVDGIRPAISALCDTHVRIPMLGAKHSLNVATAGGVVIYELLRKYRVLSATMRRA
EEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA