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The map label for this gene is dapE [H]

Identifier: 116620866

GI number: 116620866

Start: 2173345

End: 2174733

Strand: Reverse

Name: dapE [H]

Synonym: Acid_1747

Alternate gene names: 116620866

Gene position: 2174733-2173345 (Counterclockwise)

Preceding gene: 116620867

Following gene: 116620864

Centisome position: 21.82

GC content: 63.86

Gene sequence:

>1389_bases
ATGACGGGAATGCGCTTCGCACTCCTGCTGTCTCTCCTCGCACTCCCTTGCCTTGCCGCCGATAATCTGCCGCCCGCCGC
CGACCGTCAACTCGCGCGCGAGATTTACCGGCAGATGATCGAGAGCAAGTCCGGCTTCACCACCGGCGCCACCACGCCAA
TCGCCGAAGCCATGGCCGCGCGCCTCAAATCCGCCGGCTTCCCCGATGCCGATATCTTCGTCGGCGGTGCCATCCCGCGC
AAAGCAAACCTCGTGGTCCGCTATCATGGCGCTGGACGCCGCAAGCCCATCCTCCTGCTCGCCCACATCGACGTGGTTGA
AGCCCTGCGCGAAGACTGGACCACCGACCCCTTCGTCCTCATCGAAAAAGATGGCTACTTCTACGGCCGCGGCACCATGG
ACGATAAGGCCCAGGCCGCCGTCTGGATCGCCAACCTCATCCGCTACAAGCGCGAGAACTTCCGCCCCGATCGCGACCTG
ATCGTCGCCCTGACCGCCGATGAAGAAGGCGGCGGCCCCTACAACGGTGTCGATTGGCTCCTCAAGAACCACCGCGACCT
CATCGAAGCCGAGTTCGCAGTCAACGAAGGCGCGCGCGGCGAGATGATCGACGGCAAGCGCATCTCCAACGACATGTCGC
TCAGCGAGAAGGTCTACTCCGATTTCCGCCTCGAGGTTCGCAACAAGGGCGGCCACAGCGCCCGCCCCGTCCCCGACAAT
GCCATCTATCACCTCGCCGGCGGCCTCTATCGCCTGTCCACGTTCGCCTTCCCTTACCACCTCAACGAAATCACCCGCGC
CTACTTCCGACAGATGGCGAAGATCGAAAAAGGACCGCTCGCCGCTGATCTCACGCGCATCTCCCAAGGCGACGAGGCTG
CCATGCGCCGTGTCTCCGCCGCTTCCGCCCCGCTCAACGCCATGCTGCGCACGACCTGCGTCGCCACTCAGCTGGAAGGC
GGCCACGCCACGAACGCCCTGCCCCAACTCGCCGCGGCCAACGTCAACTGCCGCATCCTGCCCGAAGATTCCATTGAAAA
TGTGCTCGCCACGCTGAAGAAAGTCGTCGCCGACGATCAGGTGGTAATCACCATCAAGACCAACGAGGGCGCCAGCCCGG
CTTCCCCGATGCGCCCCGATATCATGCAGGCTTTCGAAAGAATCAGCGACATCATGTGGCCGGGCGTCATCACCGTGCCC
TCCATGGCCGTCGGCGGCTCCGACGGCCGCTACCTCCGCGTCGCCGGCATCCCCACTTACGGCATCCAGGGCTTCTTCGT
CGATCGCGACGACGTCCGCGCCCACGGGCGCGACGAGCGCATGCCCGTCAACTCCTTCTACGAAGGCCAGACCTTCCTCT
ACGAATTCGTCAAGTCTTTGTCGCAATGA

Upstream 100 bases:

>100_bases
GACGGCAAATCCTTCGCCGGCCGCTACCAGACCACCGTGGCCGATAGCGGCGACGGACTCGACGCCGGCGCCTGGAGCGC
CTCCCGCTCCAAGTGAGACA

Downstream 100 bases:

>100_bases
AGAGCCGCGACCGTCACTCGCGCTTCGCGAGCAAGGGAGCGATCTCTCTACTTCACTCCCCGGAACAGATAGCCCAGCTC
GATCACCAGGATGACCACCA

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTGMRFALLLSLLALPCLAADNLPPAADRQLAREIYRQMIESKSGFTTGATTPIAEAMAARLKSAGFPDADIFVGGAIPR
KANLVVRYHGAGRRKPILLLAHIDVVEALREDWTTDPFVLIEKDGYFYGRGTMDDKAQAAVWIANLIRYKRENFRPDRDL
IVALTADEEGGGPYNGVDWLLKNHRDLIEAEFAVNEGARGEMIDGKRISNDMSLSEKVYSDFRLEVRNKGGHSARPVPDN
AIYHLAGGLYRLSTFAFPYHLNEITRAYFRQMAKIEKGPLAADLTRISQGDEAAMRRVSAASAPLNAMLRTTCVATQLEG
GHATNALPQLAAANVNCRILPEDSIENVLATLKKVVADDQVVITIKTNEGASPASPMRPDIMQAFERISDIMWPGVITVP
SMAVGGSDGRYLRVAGIPTYGIQGFFVDRDDVRAHGRDERMPVNSFYEGQTFLYEFVKSLSQ

Sequences:

>Translated_462_residues
MTGMRFALLLSLLALPCLAADNLPPAADRQLAREIYRQMIESKSGFTTGATTPIAEAMAARLKSAGFPDADIFVGGAIPR
KANLVVRYHGAGRRKPILLLAHIDVVEALREDWTTDPFVLIEKDGYFYGRGTMDDKAQAAVWIANLIRYKRENFRPDRDL
IVALTADEEGGGPYNGVDWLLKNHRDLIEAEFAVNEGARGEMIDGKRISNDMSLSEKVYSDFRLEVRNKGGHSARPVPDN
AIYHLAGGLYRLSTFAFPYHLNEITRAYFRQMAKIEKGPLAADLTRISQGDEAAMRRVSAASAPLNAMLRTTCVATQLEG
GHATNALPQLAAANVNCRILPEDSIENVLATLKKVVADDQVVITIKTNEGASPASPMRPDIMQAFERISDIMWPGVITVP
SMAVGGSDGRYLRVAGIPTYGIQGFFVDRDDVRAHGRDERMPVNSFYEGQTFLYEFVKSLSQ
>Mature_461_residues
TGMRFALLLSLLALPCLAADNLPPAADRQLAREIYRQMIESKSGFTTGATTPIAEAMAARLKSAGFPDADIFVGGAIPRK
ANLVVRYHGAGRRKPILLLAHIDVVEALREDWTTDPFVLIEKDGYFYGRGTMDDKAQAAVWIANLIRYKRENFRPDRDLI
VALTADEEGGGPYNGVDWLLKNHRDLIEAEFAVNEGARGEMIDGKRISNDMSLSEKVYSDFRLEVRNKGGHSARPVPDNA
IYHLAGGLYRLSTFAFPYHLNEITRAYFRQMAKIEKGPLAADLTRISQGDEAAMRRVSAASAPLNAMLRTTCVATQLEGG
HATNALPQLAAANVNCRILPEDSIENVLATLKKVVADDQVVITIKTNEGASPASPMRPDIMQAFERISDIMWPGVITVPS
MAVGGSDGRYLRVAGIPTYGIQGFFVDRDDVRAHGRDERMPVNSFYEGQTFLYEFVKSLSQ

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI269846968, Length=376, Percent_Identity=28.4574468085106, Blast_Score=136, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6322289, Length=418, Percent_Identity=22.488038277512, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24649210, Length=396, Percent_Identity=24.4949494949495, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24649208, Length=382, Percent_Identity=24.6073298429319, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24649206, Length=391, Percent_Identity=22.5063938618926, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24649212, Length=309, Percent_Identity=23.9482200647249, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50897; Mature: 50766

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGMRFALLLSLLALPCLAADNLPPAADRQLAREIYRQMIESKSGFTTGATTPIAEAMAA
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RLKSAGFPDADIFVGGAIPRKANLVVRYHGAGRRKPILLLAHIDVVEALREDWTTDPFVL
HHHHCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCEEE
IEKDGYFYGRGTMDDKAQAAVWIANLIRYKRENFRPDRDLIVALTADEEGGGPYNGVDWL
EECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHH
LKNHRDLIEAEFAVNEGARGEMIDGKRISNDMSLSEKVYSDFRLEVRNKGGHSARPVPDN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCC
AIYHLAGGLYRLSTFAFPYHLNEITRAYFRQMAKIEKGPLAADLTRISQGDEAAMRRVSA
HHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHH
ASAPLNAMLRTTCVATQLEGGHATNALPQLAAANVNCRILPEDSIENVLATLKKVVADDQ
HCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCE
VVITIKTNEGASPASPMRPDIMQAFERISDIMWPGVITVPSMAVGGSDGRYLRVAGIPTY
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCEEECCCCCCEEEEECCCCC
GIQGFFVDRDDVRAHGRDERMPVNSFYEGQTFLYEFVKSLSQ
CCEEEEECHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TGMRFALLLSLLALPCLAADNLPPAADRQLAREIYRQMIESKSGFTTGATTPIAEAMAA
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RLKSAGFPDADIFVGGAIPRKANLVVRYHGAGRRKPILLLAHIDVVEALREDWTTDPFVL
HHHHCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCEEE
IEKDGYFYGRGTMDDKAQAAVWIANLIRYKRENFRPDRDLIVALTADEEGGGPYNGVDWL
EECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHH
LKNHRDLIEAEFAVNEGARGEMIDGKRISNDMSLSEKVYSDFRLEVRNKGGHSARPVPDN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCC
AIYHLAGGLYRLSTFAFPYHLNEITRAYFRQMAKIEKGPLAADLTRISQGDEAAMRRVSA
HHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHH
ASAPLNAMLRTTCVATQLEGGHATNALPQLAAANVNCRILPEDSIENVLATLKKVVADDQ
HCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCE
VVITIKTNEGASPASPMRPDIMQAFERISDIMWPGVITVPSMAVGGSDGRYLRVAGIPTY
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCEEECCCCCCEEEEECCCCC
GIQGFFVDRDDVRAHGRDERMPVNSFYEGQTFLYEFVKSLSQ
CCEEEEECHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA