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The map label for this gene is agaA [H]

Identifier: 116620708

GI number: 116620708

Start: 1948227

End: 1949783

Strand: Reverse

Name: agaA [H]

Synonym: Acid_1588

Alternate gene names: 116620708

Gene position: 1949783-1948227 (Counterclockwise)

Preceding gene: 116620710

Following gene: 116620706

Centisome position: 19.57

GC content: 62.56

Gene sequence:

>1557_bases
ATGAGATTCGCTGCACTGGCGCTGTGCGCCGCATCTGTCTATGCTGCCGATTTGACCGGACGTTGGACCGGCCCCGCCTC
GACCGCGGATAACGGTCAGGAACTGGTGCTTGCCCTCAAGCAGGCGCCGGGCGGCGGTTTCTCGGGCTACGTTCAAGGGC
CGCGCGCCACCGACACCATTGTCGGCGGCAAACTGGAAGGCGCCAACCTGACGCTCGACGTGGAACGGCCAGGCCGCAAC
AACGCAGTGCAAAAGCTCGCCTACATCGGAGTAGTGGAGGGTAACAAGCTCAAGTTGACGCTGCCCTCACCGTTCGGTGG
ACGCGGCGGAGCGCAGCAGAAGGCCCCGCCCAAGGCGCCGCCTGTTCCTCAGGTCGTGGAGTTGTCGCGCGTCTCGAACG
ATGCCCCAGCTCCCCTGCCGGCGCGCCCCGCGCCCGTATCGCTGCCCATGCCCGAGCCGGTCCACGCCAACGGCCTGGCC
AAGACCCCGCCGATGGGCTGGAACAGTTGGAACAAGTTCCGCAACCAGGTAAGCGACAAAATGGTCCGCGAAATCGCCGA
CGCCATGGTCAAGAGCGGCATGAAGGCCGCCGGGTACGTGTACGTCAACATCGACGATACCTGGGAAGGCGCGCACCGCG
ACGCACAGGGCAACATCACCACTAACAACAAATTCCCGGACATGAAAGCCCTCTCCGCCTATGTCCATTCCAAGGGACTC
AAGCTGGGCATTTACTCGTCGCCGGGACCGAAGACCTGCGCGGGCTATGAAGGCAGTTATCAGCACGAGGAACAGGACGC
CAAGACCTACGCAGCCTGGGGCATCGACTACCTCAAGTACGATTGGTGCAGCGCGTCCCAGGTCTACGACAACACCCAGG
CCACCATGGTCAATGCGTACGCCAAGATGGGACTTGGCCTGCTGAACTCCGGCCGCAAAATCGTCTACAGCCTCTGTCAG
TACGGCAATCTGGACGTCGGCGCGTGGGGAGAAAGGGTGGGCGGCAATCTGTGGCGCACCACCGGCGATATTTCGGATCG
CTGGGACTCCATGCAGCGGCTTGGCTTCGAGTTGCAAACCGATCGAGAGAAGGTCGCCGGCCCCGGCCACTGGAACGATC
CCGACATGCTGGAGATCGGCAACGGCGGCATGACGAATGACGAGTACAAAACCCACATGAGCCTGTGGTGCATTCTGGCC
TCGCCGCTGCTCGCCGGCAACGATATCCGTGACATGAAACCCGAAGTAGCCGACATCCTCATGAATAAGGAAGTCATCGC
CGTGGACCAGGACAAACTCGGGAAACAAGGCAAGCGCGTGGCCAAGGATGGCGATAAGGAAGTCTGGGCGAAGCCGCTCG
CCGACGGCAGTCACGCGGTGGCCTTGTTCAACCGCGGCGCCGACACCGCCAGCGTCACGGCCAAATGGAGCGACCTCGGC
ATCAACGGCGCCCGAACCATCCGCGACTTATGGAAACACGCGGACCTCGGCAGGAAATCCGGCGAGTTCACCGCCACCGT
CCCCAGCCACGGCGTCGTCATGGTTCGGATCAAATAA

Upstream 100 bases:

>100_bases
GAGCCACCACCCATGTTCTGATCGATCGCACGCCGGTATTGTACCGGGTCGCGCGCATGAATCCTGCTACTATCTCACGA
AGAGGCCATCATCAATGGGT

Downstream 100 bases:

>100_bases
CGAGCCGCGACCGTCAGGGAGCGCAACGCGCCTTTGATCTCCGGCATCGCCCCCAAACCGATCTGCATCTGTCTGTTGGC
AGCCCGCCAAGATTCGAGGG

Product: alpha-galactosidase

Products: NA

Alternate protein names: Alpha-D-galactoside galactohydrolase; Alpha-galactosidase 27A; Melibiase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MRFAALALCAASVYAADLTGRWTGPASTADNGQELVLALKQAPGGGFSGYVQGPRATDTIVGGKLEGANLTLDVERPGRN
NAVQKLAYIGVVEGNKLKLTLPSPFGGRGGAQQKAPPKAPPVPQVVELSRVSNDAPAPLPARPAPVSLPMPEPVHANGLA
KTPPMGWNSWNKFRNQVSDKMVREIADAMVKSGMKAAGYVYVNIDDTWEGAHRDAQGNITTNNKFPDMKALSAYVHSKGL
KLGIYSSPGPKTCAGYEGSYQHEEQDAKTYAAWGIDYLKYDWCSASQVYDNTQATMVNAYAKMGLGLLNSGRKIVYSLCQ
YGNLDVGAWGERVGGNLWRTTGDISDRWDSMQRLGFELQTDREKVAGPGHWNDPDMLEIGNGGMTNDEYKTHMSLWCILA
SPLLAGNDIRDMKPEVADILMNKEVIAVDQDKLGKQGKRVAKDGDKEVWAKPLADGSHAVALFNRGADTASVTAKWSDLG
INGARTIRDLWKHADLGRKSGEFTATVPSHGVVMVRIK

Sequences:

>Translated_518_residues
MRFAALALCAASVYAADLTGRWTGPASTADNGQELVLALKQAPGGGFSGYVQGPRATDTIVGGKLEGANLTLDVERPGRN
NAVQKLAYIGVVEGNKLKLTLPSPFGGRGGAQQKAPPKAPPVPQVVELSRVSNDAPAPLPARPAPVSLPMPEPVHANGLA
KTPPMGWNSWNKFRNQVSDKMVREIADAMVKSGMKAAGYVYVNIDDTWEGAHRDAQGNITTNNKFPDMKALSAYVHSKGL
KLGIYSSPGPKTCAGYEGSYQHEEQDAKTYAAWGIDYLKYDWCSASQVYDNTQATMVNAYAKMGLGLLNSGRKIVYSLCQ
YGNLDVGAWGERVGGNLWRTTGDISDRWDSMQRLGFELQTDREKVAGPGHWNDPDMLEIGNGGMTNDEYKTHMSLWCILA
SPLLAGNDIRDMKPEVADILMNKEVIAVDQDKLGKQGKRVAKDGDKEVWAKPLADGSHAVALFNRGADTASVTAKWSDLG
INGARTIRDLWKHADLGRKSGEFTATVPSHGVVMVRIK
>Mature_518_residues
MRFAALALCAASVYAADLTGRWTGPASTADNGQELVLALKQAPGGGFSGYVQGPRATDTIVGGKLEGANLTLDVERPGRN
NAVQKLAYIGVVEGNKLKLTLPSPFGGRGGAQQKAPPKAPPVPQVVELSRVSNDAPAPLPARPAPVSLPMPEPVHANGLA
KTPPMGWNSWNKFRNQVSDKMVREIADAMVKSGMKAAGYVYVNIDDTWEGAHRDAQGNITTNNKFPDMKALSAYVHSKGL
KLGIYSSPGPKTCAGYEGSYQHEEQDAKTYAAWGIDYLKYDWCSASQVYDNTQATMVNAYAKMGLGLLNSGRKIVYSLCQ
YGNLDVGAWGERVGGNLWRTTGDISDRWDSMQRLGFELQTDREKVAGPGHWNDPDMLEIGNGGMTNDEYKTHMSLWCILA
SPLLAGNDIRDMKPEVADILMNKEVIAVDQDKLGKQGKRVAKDGDKEVWAKPLADGSHAVALFNRGADTASVTAKWSDLG
INGARTIRDLWKHADLGRKSGEFTATVPSHGVVMVRIK

Specific function: Hydrolyzes galactomannan found in plant cell wall, by cleaving alpha-1,6-D-galactose side-chains from the mannan backbone. Appears to act in synergy with mannanase (ManA) to elicit hydrolysis of galactomannan. Has greater activity against galactomannans w

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 27 family [H]

Homologues:

Organism=Homo sapiens, GI4504009, Length=397, Percent_Identity=38.2871536523929, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI4557781, Length=387, Percent_Identity=37.4677002583979, Blast_Score=223, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17562946, Length=393, Percent_Identity=35.3689567430025, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24583253, Length=393, Percent_Identity=38.676844783715, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI19922382, Length=397, Percent_Identity=36.5239294710327, Blast_Score=237, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24654142, Length=397, Percent_Identity=36.5239294710327, Blast_Score=237, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013780
- InterPro:   IPR002241
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 55901; Mature: 55901

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00512 ALPHA_GALACTOSIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFAALALCAASVYAADLTGRWTGPASTADNGQELVLALKQAPGGGFSGYVQGPRATDTI
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCCEE
VGGKLEGANLTLDVERPGRNNAVQKLAYIGVVEGNKLKLTLPSPFGGRGGAQQKAPPKAP
ECCEECCCEEEEEECCCCCCHHHHHHHEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCC
PVPQVVELSRVSNDAPAPLPARPAPVSLPMPEPVHANGLAKTPPMGWNSWNKFRNQVSDK
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MVREIADAMVKSGMKAAGYVYVNIDDTWEGAHRDAQGNITTNNKFPDMKALSAYVHSKGL
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCC
KLGIYSSPGPKTCAGYEGSYQHEEQDAKTYAAWGIDYLKYDWCSASQVYDNTQATMVNAY
EEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEEECCCCCHHHHCCCHHHHHHHH
AKMGLGLLNSGRKIVYSLCQYGNLDVGAWGERVGGNLWRTTGDISDRWDSMQRLGFELQT
HHHCHHHHCCCHHHHHHHHHCCCCCCCCCHHHHCCCEEEECCCHHHHHHHHHHCCCEEEC
DREKVAGPGHWNDPDMLEIGNGGMTNDEYKTHMSLWCILASPLLAGNDIRDMKPEVADIL
CHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHH
MNKEVIAVDQDKLGKQGKRVAKDGDKEVWAKPLADGSHAVALFNRGADTASVTAKWSDLG
HCCCEEEECHHHHCHHHHHHHCCCCHHHHHCCCCCCCCEEEEEECCCCCCEEEEEHHHCC
INGARTIRDLWKHADLGRKSGEFTATVPSHGVVMVRIK
CCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEC
>Mature Secondary Structure
MRFAALALCAASVYAADLTGRWTGPASTADNGQELVLALKQAPGGGFSGYVQGPRATDTI
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEECCCCCCCEE
VGGKLEGANLTLDVERPGRNNAVQKLAYIGVVEGNKLKLTLPSPFGGRGGAQQKAPPKAP
ECCEECCCEEEEEECCCCCCHHHHHHHEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCC
PVPQVVELSRVSNDAPAPLPARPAPVSLPMPEPVHANGLAKTPPMGWNSWNKFRNQVSDK
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MVREIADAMVKSGMKAAGYVYVNIDDTWEGAHRDAQGNITTNNKFPDMKALSAYVHSKGL
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCC
KLGIYSSPGPKTCAGYEGSYQHEEQDAKTYAAWGIDYLKYDWCSASQVYDNTQATMVNAY
EEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEEECCCCCHHHHCCCHHHHHHHH
AKMGLGLLNSGRKIVYSLCQYGNLDVGAWGERVGGNLWRTTGDISDRWDSMQRLGFELQT
HHHCHHHHCCCHHHHHHHHHCCCCCCCCCHHHHCCCEEEECCCHHHHHHHHHHCCCEEEC
DREKVAGPGHWNDPDMLEIGNGGMTNDEYKTHMSLWCILASPLLAGNDIRDMKPEVADIL
CHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHH
MNKEVIAVDQDKLGKQGKRVAKDGDKEVWAKPLADGSHAVALFNRGADTASVTAKWSDLG
HCCCEEEECHHHHCHHHHHHHCCCCHHHHHCCCCCCCCEEEEEECCCCCCEEEEEHHHCC
INGARTIRDLWKHADLGRKSGEFTATVPSHGVVMVRIK
CCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA