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The map label for this gene is tobE [H]

Identifier: 116620678

GI number: 116620678

Start: 1915966

End: 1916919

Strand: Reverse

Name: tobE [H]

Synonym: Acid_1558

Alternate gene names: 116620678

Gene position: 1916919-1915966 (Counterclockwise)

Preceding gene: 116620680

Following gene: 116620677

Centisome position: 19.24

GC content: 65.2

Gene sequence:

>954_bases
ATGCTCGCAGTACACCTGGAAAAGGGTGCGGTAACGGTGCGCGAGGTGCCCATGCCCGCGCGGCCCGAAGGTTTCGCCCT
GCTCCGCATGCTGGCCGCGGGCATCTGCAATACAGATCTCGAGCTGCAGCGCGGCTACTACGGCTTCACCGGTACTCCCG
GACACGAGTTTGTTGCGGAGGTGGTGGAGGCGGACACCCGCGAACTCATCGGCCAGCGCGTTTCGGGCGAAATCAATCTC
GCGTGTACGCACTGCGAATGGTGCCTTCGCGGCCTGGGCCGGCATTGCCCCAACCGTACCGTGCTCGGCATCGTGGGCCA
TGCGGGCGCCTTCGCCGAGTTTCTCACGCTGCCGGAGCGCAACCTCCATATTCTGCCCGACGATTTGCCCACGAATCGCG
CCGTCTTCCTGGAGCCGCTGGCCGCCGCCTGCGAGATCCTGGACCAGGTCGCGATCCCGCCGCACGAACCGATCGCGGTG
CTGGGCGACGGCAAGCTCGGCCTGCTGATTTCGCAGGTGCTCAATGCGCACGGCTACCCGGTCCACCAATTCGGGCGCTA
CTCCGAGAAACTGGCGATCGCGCGCCGCGCCGGGATCACCACGGAACGGATTACCGGGCCGCTTCCGGTGGCTGCGTACG
ACTGGGTAGTGGATGCTACCGGGAGTACGGAGGGGCTGCGCACAGCGGTCGGGATGACCCGTCCGCGGGGAACGTTGATC
ATGAAATCGACCGTGCACGGATTGGTTCCCATCGACACGGCGCCGATCATCGTTAACGAATTGACGCTGGTGGGGTCGCG
CTGCGGGCGGATGGAGTTGGCCTTGCCGCTCATCGACCACGAGCTTATTTTGGTGGATGAGATGATCACGGACTGCTTCT
CCCTCTCCGAAGCTCCGCGTGCCTTCGCGCGGGCGGCCGAGAGGGGAGTGCTCAAGGTGCTGCTGGTTCCGTGA

Upstream 100 bases:

>100_bases
TGCGGGCAATTGTAGAGGACTTCGGTGATCGGAAACCGCGCGCGGCACCGCTCTTCAAAACAGGCTAGTTCCGCAGGCAT
GAAGATCTATTATAAAGGGG

Downstream 100 bases:

>100_bases
AAAAACGGTAGCGATTATACTGAATCGAGCGCGAACGCATTGCTGCATCGAACTCGCGCTGGGTAACCGTCCGGCTCTCC
CGATCCTCCAGGAGGTCCAC

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: DOIA dehydrogenase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MLAVHLEKGAVTVREVPMPARPEGFALLRMLAAGICNTDLELQRGYYGFTGTPGHEFVAEVVEADTRELIGQRVSGEINL
ACTHCEWCLRGLGRHCPNRTVLGIVGHAGAFAEFLTLPERNLHILPDDLPTNRAVFLEPLAAACEILDQVAIPPHEPIAV
LGDGKLGLLISQVLNAHGYPVHQFGRYSEKLAIARRAGITTERITGPLPVAAYDWVVDATGSTEGLRTAVGMTRPRGTLI
MKSTVHGLVPIDTAPIIVNELTLVGSRCGRMELALPLIDHELILVDEMITDCFSLSEAPRAFARAAERGVLKVLLVP

Sequences:

>Translated_317_residues
MLAVHLEKGAVTVREVPMPARPEGFALLRMLAAGICNTDLELQRGYYGFTGTPGHEFVAEVVEADTRELIGQRVSGEINL
ACTHCEWCLRGLGRHCPNRTVLGIVGHAGAFAEFLTLPERNLHILPDDLPTNRAVFLEPLAAACEILDQVAIPPHEPIAV
LGDGKLGLLISQVLNAHGYPVHQFGRYSEKLAIARRAGITTERITGPLPVAAYDWVVDATGSTEGLRTAVGMTRPRGTLI
MKSTVHGLVPIDTAPIIVNELTLVGSRCGRMELALPLIDHELILVDEMITDCFSLSEAPRAFARAAERGVLKVLLVP
>Mature_317_residues
MLAVHLEKGAVTVREVPMPARPEGFALLRMLAAGICNTDLELQRGYYGFTGTPGHEFVAEVVEADTRELIGQRVSGEINL
ACTHCEWCLRGLGRHCPNRTVLGIVGHAGAFAEFLTLPERNLHILPDDLPTNRAVFLEPLAAACEILDQVAIPPHEPIAV
LGDGKLGLLISQVLNAHGYPVHQFGRYSEKLAIARRAGITTERITGPLPVAAYDWVVDATGSTEGLRTAVGMTRPRGTLI
MKSTVHGLVPIDTAPIIVNELTLVGSRCGRMELALPLIDHELILVDEMITDCFSLSEAPRAFARAAERGVLKVLLVP

Specific function: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo- inosose (amino-DOI) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787863, Length=334, Percent_Identity=26.0479041916168, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI226510992, Length=286, Percent_Identity=29.020979020979, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788407, Length=348, Percent_Identity=25.2873563218391, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790045, Length=334, Percent_Identity=23.9520958083832, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788073, Length=335, Percent_Identity=24.1791044776119, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 34261; Mature: 34261

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAVHLEKGAVTVREVPMPARPEGFALLRMLAAGICNTDLELQRGYYGFTGTPGHEFVAE
CEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHH
VVEADTRELIGQRVSGEINLACTHCEWCLRGLGRHCPNRTVLGIVGHAGAFAEFLTLPER
HHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCC
NLHILPDDLPTNRAVFLEPLAAACEILDQVAIPPHEPIAVLGDGKLGLLISQVLNAHGYP
CCEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCC
VHQFGRYSEKLAIARRAGITTERITGPLPVAAYDWVVDATGSTEGLRTAVGMTRPRGTLI
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEEEEECCCCCCHHHHHHCCCCCCCEEE
MKSTVHGLVPIDTAPIIVNELTLVGSRCGRMELALPLIDHELILVDEMITDCFSLSEAPR
EEECCCCEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
AFARAAERGVLKVLLVP
HHHHHHHCCEEEEEECC
>Mature Secondary Structure
MLAVHLEKGAVTVREVPMPARPEGFALLRMLAAGICNTDLELQRGYYGFTGTPGHEFVAE
CEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHH
VVEADTRELIGQRVSGEINLACTHCEWCLRGLGRHCPNRTVLGIVGHAGAFAEFLTLPER
HHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCC
NLHILPDDLPTNRAVFLEPLAAACEILDQVAIPPHEPIAVLGDGKLGLLISQVLNAHGYP
CCEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCC
VHQFGRYSEKLAIARRAGITTERITGPLPVAAYDWVVDATGSTEGLRTAVGMTRPRGTLI
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEEEEEECCCCCCHHHHHHCCCCCCCEEE
MKSTVHGLVPIDTAPIIVNELTLVGSRCGRMELALPLIDHELILVDEMITDCFSLSEAPR
EEECCCCEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
AFARAAERGVLKVLLVP
HHHHHHHCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA