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The map label for this gene is purR [H]

Identifier: 116620600

GI number: 116620600

Start: 1815499

End: 1816539

Strand: Direct

Name: purR [H]

Synonym: Acid_1479

Alternate gene names: 116620600

Gene position: 1815499-1816539 (Clockwise)

Preceding gene: 116620599

Following gene: 116620601

Centisome position: 18.22

GC content: 64.27

Gene sequence:

>1041_bases
GTGCGCAACGCCAAAACGTCAACTCAAGGGAAGCCGGCATCCATCAAGGATGTCGCAAGACTGGCAGGCGTTGCGCACTC
CACGGTGTCGCGGGCGCTGCGCAACAGTCCGCTGGTCAACCGGGAAACGACGGAGACGATTCAGCGGATCGCCAGCGAAA
TGGGGTACCGGACGAGCGCGGCGGCGCGCAGCCTGGTGACGCGCCGCAGCGATACCATCGGCGTGGTGGTGACGAATATC
GCCGATCCGTTCATCGCCGGAGTGGTCAGCGGTATCGAGGAGACGGCGGACCAGCACGGGCTCTCGGTCTTCCTGGCAAA
TTCCAACGCGGAGCCGGAGCGCGAAGTCCGGGTGGCCCGCAAGTTCGAAGAGCGCCGGGTGGACGGAATCATCGTGACGG
CCTCGCGGGTGGGAGCGCAGTATGTGCCGCTATTGACGCACATGCAGGTGCCGATCGTGCTGCTGAACAATCAGCATCCA
AGCCAGTTCGCGCATTCCGTGATGATCGCGAACATGCAGGCCAGCCTGGATGTAACCAGGCATCTGATCCATTTGGGCCA
TCGCCGGATCGCGTACCTGGGTGACCGCCAGGGATATCAGTCGGACGCCGAGCGTTATGCCGGGTACCGGCAGGCGCTGG
AGGAGAGCCATTTGTGGGTGGACCCTCAATTGACGGTGCATGGCGACGGCAAGCCCGAGGCGGCGGAAGCCGCGGCGGGG
GAACTGCTGTCCCTGCCCGAACCTCCGACGGCGATATTCTGCTACAACGACATGTCGGCGCTGGGAGCGATGCGGCGCAT
GCGCGATCACGGGCTGCGGATTCCCGATGACATTTCGATTGTGGGCTTCGACGATCTCTATATCAGCGAGTACCTGGATC
CGCCGCTGACCACCGTGCGCCAGCCGATGCGGCAGATGGGGCGGACGGCGATGGAGACGCTGCTGCAGATCTTTGCGGGG
GCCGATTCGGGACACGACATCCGGCTGCCGGGCGAGTTGATTGTCCGCCGGACCACGGCGGCGCCGAAGGAGAAGTCATG
A

Upstream 100 bases:

>100_bases
CGTTTGGAACCATCCGAAGCGCCTTCCCGGCGCGTGTCATTCAGATGGCAATGAAGCTGGTGTTTTAGTAATGAGGCTGT
GAAGAATCGGGGCGGTAAAA

Downstream 100 bases:

>100_bases
GCGAATTGCTGATCCATCCGCGCAGTTTTACGAACCGGATTGAGAGTTTCGGTTACGAATACCTCGCGTTTGAGGCTCGC
CGGATGAAGGCGGGCGAAGA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRNAKTSTQGKPASIKDVARLAGVAHSTVSRALRNSPLVNRETTETIQRIASEMGYRTSAAARSLVTRRSDTIGVVVTNI
ADPFIAGVVSGIEETADQHGLSVFLANSNAEPEREVRVARKFEERRVDGIIVTASRVGAQYVPLLTHMQVPIVLLNNQHP
SQFAHSVMIANMQASLDVTRHLIHLGHRRIAYLGDRQGYQSDAERYAGYRQALEESHLWVDPQLTVHGDGKPEAAEAAAG
ELLSLPEPPTAIFCYNDMSALGAMRRMRDHGLRIPDDISIVGFDDLYISEYLDPPLTTVRQPMRQMGRTAMETLLQIFAG
ADSGHDIRLPGELIVRRTTAAPKEKS

Sequences:

>Translated_346_residues
MRNAKTSTQGKPASIKDVARLAGVAHSTVSRALRNSPLVNRETTETIQRIASEMGYRTSAAARSLVTRRSDTIGVVVTNI
ADPFIAGVVSGIEETADQHGLSVFLANSNAEPEREVRVARKFEERRVDGIIVTASRVGAQYVPLLTHMQVPIVLLNNQHP
SQFAHSVMIANMQASLDVTRHLIHLGHRRIAYLGDRQGYQSDAERYAGYRQALEESHLWVDPQLTVHGDGKPEAAEAAAG
ELLSLPEPPTAIFCYNDMSALGAMRRMRDHGLRIPDDISIVGFDDLYISEYLDPPLTTVRQPMRQMGRTAMETLLQIFAG
ADSGHDIRLPGELIVRRTTAAPKEKS
>Mature_346_residues
MRNAKTSTQGKPASIKDVARLAGVAHSTVSRALRNSPLVNRETTETIQRIASEMGYRTSAAARSLVTRRSDTIGVVVTNI
ADPFIAGVVSGIEETADQHGLSVFLANSNAEPEREVRVARKFEERRVDGIIVTASRVGAQYVPLLTHMQVPIVLLNNQHP
SQFAHSVMIANMQASLDVTRHLIHLGHRRIAYLGDRQGYQSDAERYAGYRQALEESHLWVDPQLTVHGDGKPEAAEAAAG
ELLSLPEPPTAIFCYNDMSALGAMRRMRDHGLRIPDDISIVGFDDLYISEYLDPPLTTVRQPMRQMGRTAMETLLQIFAG
ADSGHDIRLPGELIVRRTTAAPKEKS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=36.094674556213, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788474, Length=313, Percent_Identity=39.297124600639, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=31.1178247734139, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=33.3333333333333, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1790194, Length=308, Percent_Identity=31.8181818181818, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789068, Length=330, Percent_Identity=28.7878787878788, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1786540, Length=340, Percent_Identity=30.2941176470588, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787580, Length=330, Percent_Identity=27.5757575757576, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790715, Length=319, Percent_Identity=29.153605015674, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI48994940, Length=301, Percent_Identity=29.5681063122924, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1787906, Length=319, Percent_Identity=26.6457680250784, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789456, Length=346, Percent_Identity=25.7225433526012, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786268, Length=310, Percent_Identity=24.5161290322581, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790689, Length=320, Percent_Identity=25.625, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38063; Mature: 38063

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNAKTSTQGKPASIKDVARLAGVAHSTVSRALRNSPLVNRETTETIQRIASEMGYRTSA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHH
AARSLVTRRSDTIGVVVTNIADPFIAGVVSGIEETADQHGLSVFLANSNAEPEREVRVAR
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
KFEERRVDGIIVTASRVGAQYVPLLTHMQVPIVLLNNQHPSQFAHSVMIANMQASLDVTR
HHHHHCCCEEEEEHHHCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
HLIHLGHRRIAYLGDRQGYQSDAERYAGYRQALEESHLWVDPQLTVHGDGKPEAAEAAAG
HHHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCEECCEEEECCCCCCHHHHHHHH
ELLSLPEPPTAIFCYNDMSALGAMRRMRDHGLRIPDDISIVGFDDLYISEYLDPPLTTVR
HHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCCHHHHH
QPMRQMGRTAMETLLQIFAGADSGHDIRLPGELIVRRTTAAPKEKS
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHEEECCCCCCCCC
>Mature Secondary Structure
MRNAKTSTQGKPASIKDVARLAGVAHSTVSRALRNSPLVNRETTETIQRIASEMGYRTSA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHH
AARSLVTRRSDTIGVVVTNIADPFIAGVVSGIEETADQHGLSVFLANSNAEPEREVRVAR
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
KFEERRVDGIIVTASRVGAQYVPLLTHMQVPIVLLNNQHPSQFAHSVMIANMQASLDVTR
HHHHHCCCEEEEEHHHCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
HLIHLGHRRIAYLGDRQGYQSDAERYAGYRQALEESHLWVDPQLTVHGDGKPEAAEAAAG
HHHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCEECCEEEECCCCCCHHHHHHHH
ELLSLPEPPTAIFCYNDMSALGAMRRMRDHGLRIPDDISIVGFDDLYISEYLDPPLTTVR
HHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCCHHHHH
QPMRQMGRTAMETLLQIFAGADSGHDIRLPGELIVRRTTAAPKEKS
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA