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The map label for this gene is alaXL [H]

Identifier: 116620597

GI number: 116620597

Start: 1809565

End: 1810725

Strand: Reverse

Name: alaXL [H]

Synonym: Acid_1476

Alternate gene names: 116620597

Gene position: 1810725-1809565 (Counterclockwise)

Preceding gene: 116620611

Following gene: 116620591

Centisome position: 18.17

GC content: 67.27

Gene sequence:

>1161_bases
ATGACGGAACGCCTATACTATAAGAATTCGTACACTTCGGAGTTCCAGGCCCGGATAGTTGACCGCTCACCGGACGGTCA
AACCGTCTATTTGGACCGGAGCGCCTTCTATCCGACGTCGGGCGGGCAGCCCTTCGACCTGGGATCGATCGCAGGGATCG
CGGTGGTCGAAGTGGTGGATGAAGACGACCGCATCGCGCACCGGTTGGCTGCGCCGCTCACGACCGGCGAGGAAGTCACC
GCGCGGATCGACTGGGCGCGGCGCTTCGACCACATGCAGCAGCACTCGGGCCAGCACCTGATTTCCGCGGTTTTCGAGGA
GCTCTTCGGTCTGCGCACGGTCAGCTTCCACCTGGGCGCCGAGAGTGCCACGATCGACCTGGAAGGCGGCCCGGTGGATC
CGGGAACACTGCCGCGGGCCGAGCTGCGCGCCAACCAGTTGATTTACGAGAACCGGCCGCTCGACGTACGCTTCGAAGAC
GCGAAGGAAGCGCAGGGGCTGCGCAAAGCTTCAGAGCGCGAAGGCATGCTGCGCATTGTCTCGATCGAGGGATTGGACCG
CAGCGCCTGCGGCGGCACGCATGTCCGCGCCACCGGGGAGATCGGCGTCATCCTGCTGCGCAAGGTGGAGAAGATCCGCC
AAGCGGCGCGGGTCGAATTCGTTTGCGGCGGCCGCGCGGTGAAGCGCGCGCGCGCGGATTTCGAAGCGCTCACCAAGGTG
TCGCAGGTCTTCTCGGCGGCGCTCGACGAAGTTCCCGGGATGGTGGCGGCGCAGGTGGAAGCGGCCAAGGCCGGCGAGAA
AGCGCGGCGCAAGGTCGAACTCGAGCTCGCCGCCTACCAGGGCAAGGACCTCTACCAGGCGACCGCGCCCGGACCGGATG
GAATGCGGCGGGTGACGCAGCGGCTGGAGCGCGGCAACCTGGAGGATCTGCGCGCGGTGGCGCAGAACTTCACCGCGCAG
CCGATGGCCGTATTCCTGGCGACCCTGCAGGATCCGCCTTCGGTGCTGCTGGCCGCGTCCGCCGATTCCGGAGTGGACGC
CGGCAAAGTGCTGAAGGCTGCGCTGACCGCTGCCAGCGGACGCGGCGGCGGCAACGCGCGAATCGCACAAGGCAGCGTGC
CCGACGCCGGCGCGTTGCAGAAGGTAGTGCAGGCGGTCTGA

Upstream 100 bases:

>100_bases
GGTCGGCGTATATCATGGAGTACCCTCTCATGTAACGGACGATGATAGCTGAAATCGTGTGCAATGTGTTGTTTCAACCT
GCAAGAATATCGATAATCCT

Downstream 100 bases:

>100_bases
GCGCCTATTTTTTGCGGCTCTTGGGAGACTTCTTGACGGAAGGCTGGCCCATCAGTTTTTCGAGTTCGGCGCGTATGTTC
TTTTCGGCGGAAGTCTCTTC

Product: alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTERLYYKNSYTSEFQARIVDRSPDGQTVYLDRSAFYPTSGGQPFDLGSIAGIAVVEVVDEDDRIAHRLAAPLTTGEEVT
ARIDWARRFDHMQQHSGQHLISAVFEELFGLRTVSFHLGAESATIDLEGGPVDPGTLPRAELRANQLIYENRPLDVRFED
AKEAQGLRKASEREGMLRIVSIEGLDRSACGGTHVRATGEIGVILLRKVEKIRQAARVEFVCGGRAVKRARADFEALTKV
SQVFSAALDEVPGMVAAQVEAAKAGEKARRKVELELAAYQGKDLYQATAPGPDGMRRVTQRLERGNLEDLRAVAQNFTAQ
PMAVFLATLQDPPSVLLAASADSGVDAGKVLKAALTAASGRGGGNARIAQGSVPDAGALQKVVQAV

Sequences:

>Translated_386_residues
MTERLYYKNSYTSEFQARIVDRSPDGQTVYLDRSAFYPTSGGQPFDLGSIAGIAVVEVVDEDDRIAHRLAAPLTTGEEVT
ARIDWARRFDHMQQHSGQHLISAVFEELFGLRTVSFHLGAESATIDLEGGPVDPGTLPRAELRANQLIYENRPLDVRFED
AKEAQGLRKASEREGMLRIVSIEGLDRSACGGTHVRATGEIGVILLRKVEKIRQAARVEFVCGGRAVKRARADFEALTKV
SQVFSAALDEVPGMVAAQVEAAKAGEKARRKVELELAAYQGKDLYQATAPGPDGMRRVTQRLERGNLEDLRAVAQNFTAQ
PMAVFLATLQDPPSVLLAASADSGVDAGKVLKAALTAASGRGGGNARIAQGSVPDAGALQKVVQAV
>Mature_385_residues
TERLYYKNSYTSEFQARIVDRSPDGQTVYLDRSAFYPTSGGQPFDLGSIAGIAVVEVVDEDDRIAHRLAAPLTTGEEVTA
RIDWARRFDHMQQHSGQHLISAVFEELFGLRTVSFHLGAESATIDLEGGPVDPGTLPRAELRANQLIYENRPLDVRFEDA
KEAQGLRKASEREGMLRIVSIEGLDRSACGGTHVRATGEIGVILLRKVEKIRQAARVEFVCGGRAVKRARADFEALTKVS
QVFSAALDEVPGMVAAQVEAAKAGEKARRKVELELAAYQGKDLYQATAPGPDGMRRVTQRLERGNLEDLRAVAQNFTAQP
MAVFLATLQDPPSVLLAASADSGVDAGKVLKAALTAASGRGGGNARIAQGSVPDAGALQKVVQAV

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=311, Percent_Identity=31.1897106109325, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI217416402, Length=311, Percent_Identity=31.1897106109325, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1789048, Length=390, Percent_Identity=26.4102564102564, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18543305, Length=409, Percent_Identity=27.8728606356968, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 41581; Mature: 41449

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERLYYKNSYTSEFQARIVDRSPDGQTVYLDRSAFYPTSGGQPFDLGSIAGIAVVEVVD
CCCEEEECCCCCCCHHHEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEC
EDDRIAHRLAAPLTTGEEVTARIDWARRFDHMQQHSGQHLISAVFEELFGLRTVSFHLGA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEECC
ESATIDLEGGPVDPGTLPRAELRANQLIYENRPLDVRFEDAKEAQGLRKASEREGMLRIV
CCEEEEECCCCCCCCCCCHHHHHHHHHEECCCCCEEEECCHHHHHHHHHHHHCCCCEEEE
SIEGLDRSACGGTHVRATGEIGVILLRKVEKIRQAARVEFVCGGRAVKRARADFEALTKV
EECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHH
SQVFSAALDEVPGMVAAQVEAAKAGEKARRKVELELAAYQGKDLYQATAPGPDGMRRVTQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCEEECCCCCHHHHHHHH
RLERGNLEDLRAVAQNFTAQPMAVFLATLQDPPSVLLAASADSGVDAGKVLKAALTAASG
HHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCC
RGGGNARIAQGSVPDAGALQKVVQAV
CCCCCCEEECCCCCCHHHHHHHHHHC
>Mature Secondary Structure 
TERLYYKNSYTSEFQARIVDRSPDGQTVYLDRSAFYPTSGGQPFDLGSIAGIAVVEVVD
CCEEEECCCCCCCHHHEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEC
EDDRIAHRLAAPLTTGEEVTARIDWARRFDHMQQHSGQHLISAVFEELFGLRTVSFHLGA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEECC
ESATIDLEGGPVDPGTLPRAELRANQLIYENRPLDVRFEDAKEAQGLRKASEREGMLRIV
CCEEEEECCCCCCCCCCCHHHHHHHHHEECCCCCEEEECCHHHHHHHHHHHHCCCCEEEE
SIEGLDRSACGGTHVRATGEIGVILLRKVEKIRQAARVEFVCGGRAVKRARADFEALTKV
EECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHH
SQVFSAALDEVPGMVAAQVEAAKAGEKARRKVELELAAYQGKDLYQATAPGPDGMRRVTQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCEEECCCCCHHHHHHHH
RLERGNLEDLRAVAQNFTAQPMAVFLATLQDPPSVLLAASADSGVDAGKVLKAALTAASG
HHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCC
RGGGNARIAQGSVPDAGALQKVVQAV
CCCCCCEEECCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]