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The map label for this gene is agaA [H]

Identifier: 116620593

GI number: 116620593

Start: 1804660

End: 1806000

Strand: Direct

Name: agaA [H]

Synonym: Acid_1472

Alternate gene names: 116620593

Gene position: 1804660-1806000 (Clockwise)

Preceding gene: 116620592

Following gene: 116620594

Centisome position: 18.11

GC content: 64.5

Gene sequence:

>1341_bases
GTGCAGCCGGCTTTCAGCCGGCTTGCCCCTCTCGCAGCCGCACCCGCGATCTACACCTGTTACCGTAATCTTATGAGGAT
TGGCTTCGTTTTTTGTACCTGCCTCTTCGCCCTCTCCGCCGCCGAGCCGCCCATCGTCATCGCCCCCACTCCCCCGATGG
GCTGGAACAGCTGGGATGCCTACGGCACCAGCGTCACCGAGGCCGAAGTCAAAGCCAATGCCGATTACATGGCAGCCAAA
CTGAAATCCCACGGCTGGCAGTACATCGTGGTCGATATCCAGTGGTCGGATCCCCTCGCCAAACCCCACGGCTACCGCCC
CGACGCCGAGCTCGCCATAGACGAATTCGGCCGCCTCATTCCCGCCGTCAACCGCTTCCCCGCGGGCTTTAAAGCTCTCG
GCGACTACCTTCACGCCCGCGGCCTGAAGTTCGGCATCCACATCATGCGCGGCATTCCGCGCCGGGCCGTCGCCGCCAAC
CTGCCCATCGCCGGCAGCAAAGCCCGCGCCGCCGATATCGCCGACACCAAGTCCATCTGCCGCTGGAATACCGATATGTA
CGGCATCGACATGTCCAAGCCCGGAGCCCAGGATTACTATGATTCCATCCTCACCCTTTACGCCGGGTGGGGCGTCGATT
TCATCAAGTGTGACGACATCGCGCAGCCCTTCCACGGCGACGAAATCGCCGCCCTCCACAAGGCCATCCTCCGCACAAAA
CGCCCCATCGTGCTCAGCCTCTCTCCCGGCCCCGCCGAGCTGAGCAAGGCAGCCTTCTACGCCGCCAACGCCAATCTCTG
GCGCATCTCCGGCGATTTCTGGGATCTCTGGACCGATCTCCGTAAGACCTTCGACCTGTTGGACAAATGGAGCCCGTACG
TCCAGCCGGGCGGCTGGCCCGATGCCGACATGCTCCCGCTCGGCCGCATCGGCATCCGCGCCGAACGCGGCAATGACCGC
CGCACCCGCTTCACCCCGGCGGAGCAGCGCACGCTCATGACCCTCTGGTCGATCGCCCGCAGCCCACTGATGTTCGGCGG
CGATCTGCCCAGCAACGACGCCGCGACCGAGGCACTCCTCACCAACGACGAAGTCCTCGCAGCCAATCAGAAGGGTACCC
ACTCCCGCAAGCTATCCCAACAGGGCGATGCCGTCATTTGGACATCCGATGTCGGCGGCCGGCAGTATTTCGCCGCCTTC
AACCTCGGAGAAATCCCGGACCTCCTGATCACCATCCCTGGCACTGGCCCCTTGCGCGACGTCTGGGAGCACCGCGACCT
GACCAACCGCACCCTCCGCCTCGAGCCCCACTCCGCCGCCCTCGTGGTCCACGTACCCTGA

Upstream 100 bases:

>100_bases
GCGGCTTCTTGCGGAGACCGCCAGCGCCTCCAAATTGCGCGTCAGCTACGCCGCGCTTTTCGAGGAGTATTCCGTTGTTC
GCCGTGGGGGTTTTCGACCC

Downstream 100 bases:

>100_bases
CAACGAGCCGCGACAGTCACGAAGCGCTACCCGCGCACCTGCACCCGCAGCTGCCCGCTACACGTCGCTGCCTCGCGAAC
CGCGACCGTTAGGGAGCGAT

Product: glycoside hydrolase, clan GH-D

Products: NA

Alternate protein names: Alpha-D-galactoside galactohydrolase; Alpha-galactosidase 27A; Melibiase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MQPAFSRLAPLAAAPAIYTCYRNLMRIGFVFCTCLFALSAAEPPIVIAPTPPMGWNSWDAYGTSVTEAEVKANADYMAAK
LKSHGWQYIVVDIQWSDPLAKPHGYRPDAELAIDEFGRLIPAVNRFPAGFKALGDYLHARGLKFGIHIMRGIPRRAVAAN
LPIAGSKARAADIADTKSICRWNTDMYGIDMSKPGAQDYYDSILTLYAGWGVDFIKCDDIAQPFHGDEIAALHKAILRTK
RPIVLSLSPGPAELSKAAFYAANANLWRISGDFWDLWTDLRKTFDLLDKWSPYVQPGGWPDADMLPLGRIGIRAERGNDR
RTRFTPAEQRTLMTLWSIARSPLMFGGDLPSNDAATEALLTNDEVLAANQKGTHSRKLSQQGDAVIWTSDVGGRQYFAAF
NLGEIPDLLITIPGTGPLRDVWEHRDLTNRTLRLEPHSAALVVHVP

Sequences:

>Translated_446_residues
MQPAFSRLAPLAAAPAIYTCYRNLMRIGFVFCTCLFALSAAEPPIVIAPTPPMGWNSWDAYGTSVTEAEVKANADYMAAK
LKSHGWQYIVVDIQWSDPLAKPHGYRPDAELAIDEFGRLIPAVNRFPAGFKALGDYLHARGLKFGIHIMRGIPRRAVAAN
LPIAGSKARAADIADTKSICRWNTDMYGIDMSKPGAQDYYDSILTLYAGWGVDFIKCDDIAQPFHGDEIAALHKAILRTK
RPIVLSLSPGPAELSKAAFYAANANLWRISGDFWDLWTDLRKTFDLLDKWSPYVQPGGWPDADMLPLGRIGIRAERGNDR
RTRFTPAEQRTLMTLWSIARSPLMFGGDLPSNDAATEALLTNDEVLAANQKGTHSRKLSQQGDAVIWTSDVGGRQYFAAF
NLGEIPDLLITIPGTGPLRDVWEHRDLTNRTLRLEPHSAALVVHVP
>Mature_446_residues
MQPAFSRLAPLAAAPAIYTCYRNLMRIGFVFCTCLFALSAAEPPIVIAPTPPMGWNSWDAYGTSVTEAEVKANADYMAAK
LKSHGWQYIVVDIQWSDPLAKPHGYRPDAELAIDEFGRLIPAVNRFPAGFKALGDYLHARGLKFGIHIMRGIPRRAVAAN
LPIAGSKARAADIADTKSICRWNTDMYGIDMSKPGAQDYYDSILTLYAGWGVDFIKCDDIAQPFHGDEIAALHKAILRTK
RPIVLSLSPGPAELSKAAFYAANANLWRISGDFWDLWTDLRKTFDLLDKWSPYVQPGGWPDADMLPLGRIGIRAERGNDR
RTRFTPAEQRTLMTLWSIARSPLMFGGDLPSNDAATEALLTNDEVLAANQKGTHSRKLSQQGDAVIWTSDVGGRQYFAAF
NLGEIPDLLITIPGTGPLRDVWEHRDLTNRTLRLEPHSAALVVHVP

Specific function: Hydrolyzes galactomannan found in plant cell wall, by cleaving alpha-1,6-D-galactose side-chains from the mannan backbone. Appears to act in synergy with mannanase (ManA) to elicit hydrolysis of galactomannan. Has greater activity against galactomannans w

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 27 family [H]

Homologues:

Organism=Homo sapiens, GI4504009, Length=390, Percent_Identity=27.1794871794872, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24583253, Length=371, Percent_Identity=26.9541778975741, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013780
- InterPro:   IPR002241
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 49303; Mature: 49303

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPAFSRLAPLAAAPAIYTCYRNLMRIGFVFCTCLFALSAAEPPIVIAPTPPMGWNSWDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
YGTSVTEAEVKANADYMAAKLKSHGWQYIVVDIQWSDPLAKPHGYRPDAELAIDEFGRLI
CCCCEEHHHHHCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
PAVNRFPAGFKALGDYLHARGLKFGIHIMRGIPRRAVAANLPIAGSKARAADIADTKSIC
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHH
RWNTDMYGIDMSKPGAQDYYDSILTLYAGWGVDFIKCDDIAQPFHGDEIAALHKAILRTK
HCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCC
RPIVLSLSPGPAELSKAAFYAANANLWRISGDFWDLWTDLRKTFDLLDKWSPYVQPGGWP
CCEEEEECCCHHHHHHHEEEEECCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DADMLPLGRIGIRAERGNDRRTRFTPAEQRTLMTLWSIARSPLMFGGDLPSNDAATEALL
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHC
TNDEVLAANQKGTHSRKLSQQGDAVIWTSDVGGRQYFAAFNLGEIPDLLITIPGTGPLRD
CCCCEEECCCCCCHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCCHHH
VWEHRDLTNRTLRLEPHSAALVVHVP
HHHHCCCCCCEEEECCCCEEEEEECC
>Mature Secondary Structure
MQPAFSRLAPLAAAPAIYTCYRNLMRIGFVFCTCLFALSAAEPPIVIAPTPPMGWNSWDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
YGTSVTEAEVKANADYMAAKLKSHGWQYIVVDIQWSDPLAKPHGYRPDAELAIDEFGRLI
CCCCEEHHHHHCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
PAVNRFPAGFKALGDYLHARGLKFGIHIMRGIPRRAVAANLPIAGSKARAADIADTKSIC
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHH
RWNTDMYGIDMSKPGAQDYYDSILTLYAGWGVDFIKCDDIAQPFHGDEIAALHKAILRTK
HCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCC
RPIVLSLSPGPAELSKAAFYAANANLWRISGDFWDLWTDLRKTFDLLDKWSPYVQPGGWP
CCEEEEECCCHHHHHHHEEEEECCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DADMLPLGRIGIRAERGNDRRTRFTPAEQRTLMTLWSIARSPLMFGGDLPSNDAATEALL
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHC
TNDEVLAANQKGTHSRKLSQQGDAVIWTSDVGGRQYFAAFNLGEIPDLLITIPGTGPLRD
CCCCEEECCCCCCHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCCHHH
VWEHRDLTNRTLRLEPHSAALVVHVP
HHHHCCCCCCEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA