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The map label for this gene is oprF [H]

Identifier: 116620548

GI number: 116620548

Start: 1753430

End: 1754218

Strand: Reverse

Name: oprF [H]

Synonym: Acid_1426

Alternate gene names: 116620548

Gene position: 1754218-1753430 (Counterclockwise)

Preceding gene: 116620549

Following gene: 116620543

Centisome position: 17.6

GC content: 58.94

Gene sequence:

>789_bases
ATGAGAGTAATTCTCGCGACCACTTGCATCCTGACGGGGGCGTTGCTCGCCGGCGGATGCGCAACGAAGAAGTATGTGCG
CAACACTACCGCGCCAGTCCAGGCGAAGGTGGATCAGGTAGGCGACCAGACTACCAGAAACGGCCAGCAGATTGAGGACA
CTCGCAACCAGGTGAAGCAGGTTGACGAGAGGGCGCAGAACGGCATCAGCGCGGCGCAGGAACGCGCATCGAGTGCCGAT
CAGCACGCCGCAACGGCGGACCAGCACGCCGGCGAGGCGATGACTCGCGCCAATCAGGCCAACCAATTGGGGGAACAGAA
CACTCAGGCTTTGAACAGTCTGAAGAGCGTGGTTTCTAATATCGACGACTACAAACTGCAGACTTCGGTAGCCGTCCCGT
TCCAATTCAACAAATACAATCTCTCCTCCGATGCAAAACAGGATCTCGACAAGCTGGCGAGTGACGTGAAGAGCGACAAG
CGCTACTTCATCGCGGTGGAAGGCTACACCGACAAGACCGGATCCAAGGCATATAACTCCGCATTAAGCCGCCGCAGGGC
GGATGCGGTGGTCGAATACCTGGTAGCACAGCATGACGTGCCGATCTACCGGATCCACATGATTGGCTTGGGCGACGAGA
AGCCGGTGGATGAAGCGAAGAACCGGGCGGCGCGAGCCAAGAACCGCCGCGTGGAAGTGAAAGTGTTCAGCGCGGACCAG
GTGATGGCCGGAATTAGCAGCGACGCGGGTGCCAGCACTGCCAACCGGCAGCAGGAAGGTAAGCAGTAG

Upstream 100 bases:

>100_bases
TCGTGGCCCCGCCCACATTAAATTGGTTGATGTTATTGAAAATGGTGGGATTACAACGATGGCTTGAGGATTGCATAAAG
CGAGAACGAGGTTTGACACC

Downstream 100 bases:

>100_bases
CGGGACATTCGTTTGGAAGTTCCTCCGGCAAGAAAAGGGGCTGCATCGAGAGATGCAGCCCTTTTTTTATGGTCGGCGCG
CGGCTGGACCGTACCGTCCT

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRVILATTCILTGALLAGGCATKKYVRNTTAPVQAKVDQVGDQTTRNGQQIEDTRNQVKQVDERAQNGISAAQERASSAD
QHAATADQHAGEAMTRANQANQLGEQNTQALNSLKSVVSNIDDYKLQTSVAVPFQFNKYNLSSDAKQDLDKLASDVKSDK
RYFIAVEGYTDKTGSKAYNSALSRRRADAVVEYLVAQHDVPIYRIHMIGLGDEKPVDEAKNRAARAKNRRVEVKVFSADQ
VMAGISSDAGASTANRQQEGKQ

Sequences:

>Translated_262_residues
MRVILATTCILTGALLAGGCATKKYVRNTTAPVQAKVDQVGDQTTRNGQQIEDTRNQVKQVDERAQNGISAAQERASSAD
QHAATADQHAGEAMTRANQANQLGEQNTQALNSLKSVVSNIDDYKLQTSVAVPFQFNKYNLSSDAKQDLDKLASDVKSDK
RYFIAVEGYTDKTGSKAYNSALSRRRADAVVEYLVAQHDVPIYRIHMIGLGDEKPVDEAKNRAARAKNRRVEVKVFSADQ
VMAGISSDAGASTANRQQEGKQ
>Mature_262_residues
MRVILATTCILTGALLAGGCATKKYVRNTTAPVQAKVDQVGDQTTRNGQQIEDTRNQVKQVDERAQNGISAAQERASSAD
QHAATADQHAGEAMTRANQANQLGEQNTQALNSLKSVVSNIDDYKLQTSVAVPFQFNKYNLSSDAKQDLDKLASDVKSDK
RYFIAVEGYTDKTGSKAYNSALSRRRADAVVEYLVAQHDVPIYRIHMIGLGDEKPVDEAKNRAARAKNRRVEVKVFSADQ
VMAGISSDAGASTANRQQEGKQ

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1787191, Length=115, Percent_Identity=40.8695652173913, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786962, Length=99, Percent_Identity=39.3939393939394, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788957, Length=113, Percent_Identity=30.9734513274336, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 28491; Mature: 28491

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILATTCILTGALLAGGCATKKYVRNTTAPVQAKVDQVGDQTTRNGQQIEDTRNQVKQ
CEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCHHHHHHHHHHH
VDERAQNGISAAQERASSADQHAATADQHAGEAMTRANQANQLGEQNTQALNSLKSVVSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IDDYKLQTSVAVPFQFNKYNLSSDAKQDLDKLASDVKSDKRYFIAVEGYTDKTGSKAYNS
CCCCEEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHH
ALSRRRADAVVEYLVAQHDVPIYRIHMIGLGDEKPVDEAKNRAARAKNRRVEVKVFSADQ
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEECHHH
VMAGISSDAGASTANRQQEGKQ
HHHCCCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MRVILATTCILTGALLAGGCATKKYVRNTTAPVQAKVDQVGDQTTRNGQQIEDTRNQVKQ
CEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCHHHHHHHHHHH
VDERAQNGISAAQERASSADQHAATADQHAGEAMTRANQANQLGEQNTQALNSLKSVVSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IDDYKLQTSVAVPFQFNKYNLSSDAKQDLDKLASDVKSDKRYFIAVEGYTDKTGSKAYNS
CCCCEEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHH
ALSRRRADAVVEYLVAQHDVPIYRIHMIGLGDEKPVDEAKNRAARAKNRRVEVKVFSADQ
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEECHHH
VMAGISSDAGASTANRQQEGKQ
HHHCCCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]