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The map label for this gene is thiC

Identifier: 116620522

GI number: 116620522

Start: 1726805

End: 1728190

Strand: Direct

Name: thiC

Synonym: Acid_1400

Alternate gene names: 116620522

Gene position: 1726805-1728190 (Clockwise)

Preceding gene: 116620521

Following gene: 116620523

Centisome position: 17.33

GC content: 60.17

Gene sequence:

>1386_bases
ATGCGCACAGACTGGGTAGCCAAACGTAAGAACGATCCGATTCGCACGCAAATGCACTATGCCCGCAAGGGCATTATCAC
CGAGGAAATGCACTACATCGCGAAGCGCGAGAGCATTTCTCCGGAACTGATACGCAGCGAAGTGGCGCGCGGCCGCATGA
TCATCCCGGCCAACGTTAATCACGCCAACCTGGAGCCGATGTGCATCGGCGTGGCTTCCAAGTGCAAGATCAACTCGAAT
ATCGGCAACTCGTCCGTGGTCAGCGATATTGCGGGCGAACTGGAAAAACTGGAGTACTCGGTAAAGTACGGCGCCGACAC
CGTGATGGATCTCTCCACCGGCGGCGATATCCCTGCGATCCGAAAAGCGATCATCGGCGCTTCGCCGATCCCGATCGGGA
CGGTGCCGATTTACGAGGCCCTTTCGCGCGTGCGCCGCGTGGAAGATCTCTCGGCTCAGGTAATGCTGGAAGTGATCGAA
GAGCAGGCCGAGCAGGGCGTGGACTATATGACAATCCATGCCGGCATCCTGGTGCAGCACATTCCGCTGACCACGAAGCG
CGTCACCGGCATCGTGAGCCGCGGCGGGTCGATTTTGGCGGAGTGGATGGTCAAGAATCACAAGCAGAATTTCCTGTACG
AGTGTTTTGAGGACATCTGCAAGATTCTCCAAAAACACGATGTGAGCTTCTCGCTGGGCGACGGTCTTCGTCCCGGCAGC
ATTGCCGACGCAAGCGACGCGGCGCAGTTTGCCGAACTCAAGACCTTGGGCGAGCTGACGCAGGTCGCCTGGAAGCACGA
CGTGCAGACCATGATCGAAGGTCCGGGGCACATTCCGATGGACCAGATCGAAATGCAGGTGATCAAGGAAAAGGAGCTGT
GCTTCGAAGCGCCGTTCTATACGCTCGGACCTCTGGTCACCGATATCGCGCCTGGGTACGACCACATCACGAGTGCCATC
GGGGCCGCCATGATCGGCTGGTATGGCGCCAGCATGCTCTGTTACGTGACGCCGAAGGAACACCTGGGGCTGCCCAATCG
CGAAGACGTGAAGGCGGGCATCATCGCGTATAAGATCGCCGCGCACGCCGCGGATATCGCGCGTCACCGCCCGGGCGTGC
GCGACCGCGACGACGCGCTATCCCGCGCACGATATCGCTTCGACTGGAAGGAGCAGTTCGCGCTTTCGCTGGACCCCGAA
ACCGCGCGCTCCATGCACGATGAGACTCTCCCGGAGGACGGCTTCAAGGACGCGCATTTCTGCTCGATGTGCGGGCCGAA
GTTCTGCTCCATGAACATCTCCGCGAAGGTCGAGTCGTTCACGGCCGACGATGCGGCGGCGGTACTCAGCGGTGCCGCGC
CGGAGAGCCTGGTCAACATCGAATAG

Upstream 100 bases:

>100_bases
AGCCCAAGAAAAAGAAGTAACCGGGGACCAGGTTTTACCAGCGGGCGCGCTTCAGTGCGCGCCCGCCGCCATCAGGAAGT
CAATATCAGGGGAGGCCGTC

Downstream 100 bases:

>100_bases
GCGTTGTTACGCTCCCTCACGGTCGCATCTCGTTGAGAGGCTGTTCTGGAAGTGCGATTGCATCGTTCCGGGCGTCAGGA
GGAATGATGAAGAAACGTTG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRTDWVAKRKNDPIRTQMHYARKGIITEEMHYIAKRESISPELIRSEVARGRMIIPANVNHANLEPMCIGVASKCKINSN
IGNSSVVSDIAGELEKLEYSVKYGADTVMDLSTGGDIPAIRKAIIGASPIPIGTVPIYEALSRVRRVEDLSAQVMLEVIE
EQAEQGVDYMTIHAGILVQHIPLTTKRVTGIVSRGGSILAEWMVKNHKQNFLYECFEDICKILQKHDVSFSLGDGLRPGS
IADASDAAQFAELKTLGELTQVAWKHDVQTMIEGPGHIPMDQIEMQVIKEKELCFEAPFYTLGPLVTDIAPGYDHITSAI
GAAMIGWYGASMLCYVTPKEHLGLPNREDVKAGIIAYKIAAHAADIARHRPGVRDRDDALSRARYRFDWKEQFALSLDPE
TARSMHDETLPEDGFKDAHFCSMCGPKFCSMNISAKVESFTADDAAAVLSGAAPESLVNIE

Sequences:

>Translated_461_residues
MRTDWVAKRKNDPIRTQMHYARKGIITEEMHYIAKRESISPELIRSEVARGRMIIPANVNHANLEPMCIGVASKCKINSN
IGNSSVVSDIAGELEKLEYSVKYGADTVMDLSTGGDIPAIRKAIIGASPIPIGTVPIYEALSRVRRVEDLSAQVMLEVIE
EQAEQGVDYMTIHAGILVQHIPLTTKRVTGIVSRGGSILAEWMVKNHKQNFLYECFEDICKILQKHDVSFSLGDGLRPGS
IADASDAAQFAELKTLGELTQVAWKHDVQTMIEGPGHIPMDQIEMQVIKEKELCFEAPFYTLGPLVTDIAPGYDHITSAI
GAAMIGWYGASMLCYVTPKEHLGLPNREDVKAGIIAYKIAAHAADIARHRPGVRDRDDALSRARYRFDWKEQFALSLDPE
TARSMHDETLPEDGFKDAHFCSMCGPKFCSMNISAKVESFTADDAAAVLSGAAPESLVNIE
>Mature_461_residues
MRTDWVAKRKNDPIRTQMHYARKGIITEEMHYIAKRESISPELIRSEVARGRMIIPANVNHANLEPMCIGVASKCKINSN
IGNSSVVSDIAGELEKLEYSVKYGADTVMDLSTGGDIPAIRKAIIGASPIPIGTVPIYEALSRVRRVEDLSAQVMLEVIE
EQAEQGVDYMTIHAGILVQHIPLTTKRVTGIVSRGGSILAEWMVKNHKQNFLYECFEDICKILQKHDVSFSLGDGLRPGS
IADASDAAQFAELKTLGELTQVAWKHDVQTMIEGPGHIPMDQIEMQVIKEKELCFEAPFYTLGPLVTDIAPGYDHITSAI
GAAMIGWYGASMLCYVTPKEHLGLPNREDVKAGIIAYKIAAHAADIARHRPGVRDRDDALSRARYRFDWKEQFALSLDPE
TARSMHDETLPEDGFKDAHFCSMCGPKFCSMNISAKVESFTADDAAAVLSGAAPESLVNIE

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=455, Percent_Identity=60, Blast_Score=573, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_SOLUE (Q028Z8)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_822678.1
- STRING:   Q028Z8
- GeneID:   4430428
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_1400
- NMPDR:   fig|234267.9.peg.1332
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   EPMAIGI
- PhylomeDB:   Q028Z8
- ProtClustDB:   PRK13352
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 50799; Mature: 50799

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: BINDING 80-80 BINDING 109-109 BINDING 138-138 BINDING 173-173 BINDING 273-273 BINDING 300-300

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTDWVAKRKNDPIRTQMHYARKGIITEEMHYIAKRESISPELIRSEVARGRMIIPANVN
CCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HANLEPMCIGVASKCKINSNIGNSSVVSDIAGELEKLEYSVKYGADTVMDLSTGGDIPAI
CCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
RKAIIGASPIPIGTVPIYEALSRVRRVEDLSAQVMLEVIEEQAEQGVDYMTIHAGILVQH
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
IPLTTKRVTGIVSRGGSILAEWMVKNHKQNFLYECFEDICKILQKHDVSFSLGDGLRPGS
CCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
IADASDAAQFAELKTLGELTQVAWKHDVQTMIEGPGHIPMDQIEMQVIKEKELCFEAPFY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCH
TLGPLVTDIAPGYDHITSAIGAAMIGWYGASMLCYVTPKEHLGLPNREDVKAGIIAYKIA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHH
AHAADIARHRPGVRDRDDALSRARYRFDWKEQFALSLDPETARSMHDETLPEDGFKDAHF
HHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHEEEECCCHHHHHHHHHCCCCCCCCCCHH
CSMCGPKFCSMNISAKVESFTADDAAAVLSGAAPESLVNIE
HHHCCCHHCCCCCCHHEHHCCCCHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MRTDWVAKRKNDPIRTQMHYARKGIITEEMHYIAKRESISPELIRSEVARGRMIIPANVN
CCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HANLEPMCIGVASKCKINSNIGNSSVVSDIAGELEKLEYSVKYGADTVMDLSTGGDIPAI
CCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
RKAIIGASPIPIGTVPIYEALSRVRRVEDLSAQVMLEVIEEQAEQGVDYMTIHAGILVQH
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
IPLTTKRVTGIVSRGGSILAEWMVKNHKQNFLYECFEDICKILQKHDVSFSLGDGLRPGS
CCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
IADASDAAQFAELKTLGELTQVAWKHDVQTMIEGPGHIPMDQIEMQVIKEKELCFEAPFY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCH
TLGPLVTDIAPGYDHITSAIGAAMIGWYGASMLCYVTPKEHLGLPNREDVKAGIIAYKIA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHH
AHAADIARHRPGVRDRDDALSRARYRFDWKEQFALSLDPETARSMHDETLPEDGFKDAHF
HHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHEEEECCCHHHHHHHHHCCCCCCCCCCHH
CSMCGPKFCSMNISAKVESFTADDAAAVLSGAAPESLVNIE
HHHCCCHHCCCCCCHHEHHCCCCHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA