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The map label for this gene is lcfB [H]

Identifier: 116620485

GI number: 116620485

Start: 1678519

End: 1679862

Strand: Direct

Name: lcfB [H]

Synonym: Acid_1363

Alternate gene names: 116620485

Gene position: 1678519-1679862 (Clockwise)

Preceding gene: 116620484

Following gene: 116620486

Centisome position: 16.84

GC content: 63.91

Gene sequence:

>1344_bases
ATGTTTCTGCAAGACGCGTCGATTCCCGAATCCAACCTCGCGCTGTTCGATGCGGCGCAGGCGCGCTGGTATAGCTACGG
CGAGTTGTGGAGCGAAGTGGCGCGCACGGCGGAGTCGCTGAAGGGGCCGAAGGCGCTGGCCTTCTGCTTCTGCCGGAATA
ACATCGCATCGGTGGTGTGGTATCTGGCCGCCGTAGAGGCAGGGCACTCGGTGGCGCTGCTGGACGACGGGCTGACTCCG
GAGTTCAAGACGCGGCTGATCGAACTGTACGACCCGGAACTGATCCAGGAGGCGGGCCAGCACGAGTGGCGGCGCACAAA
CGCGGCCGGCGGAACGATCGCGTCGGAACTCGCGGTGCTGCTTTCGACTTCGGGCAGCACCGGCAGTCCAAAATTCGTTC
GCTTGACTGCGGAGAACGTCTCGGCCAATGCGCGCTCGATCTCGGAAGCGCTGGCCATCGGCGCGGCGGACCGCGCGATC
AGCAGCCTGCCGATGCACTATTCCTACGGGCTTTCGGTGCTCAACACGCACCTGCTGCGGGGAGCGTCGATGGTGTTGAC
CAACGACGGGCTGATGTCGCCCAATTTCTGGAAGCTGTTTCGCGAACAGGAGTGCACATCGTTCGCGGGCGTGCCGTACT
CGTACCAGATGCTGCGGCGATTGGGGATCGACGGACTGAACGTGCCGAGCCTGCGCACCATGACACAGGCGGGCGGCAAG
CTGCACACCGACCTGGTGGCGCATTTTCACGAAAAGATGGTGGAGCGCGGCGGCCGGTTCTTCGTGATGTACGGACAGAC
GGAGGCCACGGCACGCATCGCGATATTGCCGCACGACCGGCTGCCGGAGAAGTTGGGATCGGCCGGCGTCGCAATTCCGG
GCGGACGCTTTCACATTGAAAGCGATGGCAGGGAAAGCGGAGAGCTGATCTACACGGGTCCAAACGTGATGATGGGATAC
GCCACCTGCCGCGAGGATCTCGCGCTGGGCGACGTGCTGCAAGGCAGGCTCGCAACCGGCGACCTGGCGCGGCTGGATTC
CGAAGGCTTCGTCACTATCGAGGGCCGGATGAAGCGCGACGCCAAGCTGTTCGGGCTGCGCATCAACCTGGACGAGATCG
AGAGCATGCTGCGCGCGCACGGTCCCACGGCGGTAATCAGCGGACCCGACAAGCTGTTGATTTATTGCGAACACGGGGAC
GAGGCGGCCTTTGCGCGCTACCGCACGGATCTGGCTGCGAAGCTGAAGGTGAACCATCGCGCGCTGGAGTTTTTCCGCCT
GGAGCGTCTGCCGGTCAATGGCAGCGGGAAGATCGATTACCAGGCGCTGGCGGGCGCGCGATGA

Upstream 100 bases:

>100_bases
ACGTGGCCAATGCGATCCTGTTTCTGGCTTCGGACCTGTCGAGTTACGTAACCGGGCAGGTGCTCGGCGTCGATGGCGGC
ATGTTGATTTGAGAGCGGAC

Downstream 100 bases:

>100_bases
ACTTCGCCGAGCTGCTGCAATCGCCGCAATACAGCATTCCACAGGCTCGGAAGGAAGCGCTGCTGCTGGAGCATCTCAAT
GAGCTCGACCGGATGCATCG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MFLQDASIPESNLALFDAAQARWYSYGELWSEVARTAESLKGPKALAFCFCRNNIASVVWYLAAVEAGHSVALLDDGLTP
EFKTRLIELYDPELIQEAGQHEWRRTNAAGGTIASELAVLLSTSGSTGSPKFVRLTAENVSANARSISEALAIGAADRAI
SSLPMHYSYGLSVLNTHLLRGASMVLTNDGLMSPNFWKLFREQECTSFAGVPYSYQMLRRLGIDGLNVPSLRTMTQAGGK
LHTDLVAHFHEKMVERGGRFFVMYGQTEATARIAILPHDRLPEKLGSAGVAIPGGRFHIESDGRESGELIYTGPNVMMGY
ATCREDLALGDVLQGRLATGDLARLDSEGFVTIEGRMKRDAKLFGLRINLDEIESMLRAHGPTAVISGPDKLLIYCEHGD
EAAFARYRTDLAAKLKVNHRALEFFRLERLPVNGSGKIDYQALAGAR

Sequences:

>Translated_447_residues
MFLQDASIPESNLALFDAAQARWYSYGELWSEVARTAESLKGPKALAFCFCRNNIASVVWYLAAVEAGHSVALLDDGLTP
EFKTRLIELYDPELIQEAGQHEWRRTNAAGGTIASELAVLLSTSGSTGSPKFVRLTAENVSANARSISEALAIGAADRAI
SSLPMHYSYGLSVLNTHLLRGASMVLTNDGLMSPNFWKLFREQECTSFAGVPYSYQMLRRLGIDGLNVPSLRTMTQAGGK
LHTDLVAHFHEKMVERGGRFFVMYGQTEATARIAILPHDRLPEKLGSAGVAIPGGRFHIESDGRESGELIYTGPNVMMGY
ATCREDLALGDVLQGRLATGDLARLDSEGFVTIEGRMKRDAKLFGLRINLDEIESMLRAHGPTAVISGPDKLLIYCEHGD
EAAFARYRTDLAAKLKVNHRALEFFRLERLPVNGSGKIDYQALAGAR
>Mature_447_residues
MFLQDASIPESNLALFDAAQARWYSYGELWSEVARTAESLKGPKALAFCFCRNNIASVVWYLAAVEAGHSVALLDDGLTP
EFKTRLIELYDPELIQEAGQHEWRRTNAAGGTIASELAVLLSTSGSTGSPKFVRLTAENVSANARSISEALAIGAADRAI
SSLPMHYSYGLSVLNTHLLRGASMVLTNDGLMSPNFWKLFREQECTSFAGVPYSYQMLRRLGIDGLNVPSLRTMTQAGGK
LHTDLVAHFHEKMVERGGRFFVMYGQTEATARIAILPHDRLPEKLGSAGVAIPGGRFHIESDGRESGELIYTGPNVMMGY
ATCREDLALGDVLQGRLATGDLARLDSEGFVTIEGRMKRDAKLFGLRINLDEIESMLRAHGPTAVISGPDKLLIYCEHGD
EAAFARYRTDLAAKLKVNHRALEFFRLERLPVNGSGKIDYQALAGAR

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1789201, Length=350, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI145693145, Length=278, Percent_Identity=29.4964028776978, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788107, Length=317, Percent_Identity=27.1293375394322, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786810, Length=382, Percent_Identity=23.2984293193717, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559526, Length=310, Percent_Identity=26.7741935483871, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=363, Percent_Identity=25.6198347107438, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71996755, Length=341, Percent_Identity=25.2199413489736, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=387, Percent_Identity=24.031007751938, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18859661, Length=352, Percent_Identity=23.8636363636364, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24581924, Length=352, Percent_Identity=28.125, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 48933; Mature: 48933

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLQDASIPESNLALFDAAQARWYSYGELWSEVARTAESLKGPKALAFCFCRNNIASVVW
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEHHCCHHHHHH
YLAAVEAGHSVALLDDGLTPEFKTRLIELYDPELIQEAGQHEWRRTNAAGGTIASELAVL
HHHHHHCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHCHHHHHHCCCCCCHHHHHHHHH
LSTSGSTGSPKFVRLTAENVSANARSISEALAIGAADRAISSLPMHYSYGLSVLNTHLLR
HCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHC
GASMVLTNDGLMSPNFWKLFREQECTSFAGVPYSYQMLRRLGIDGLNVPSLRTMTQAGGK
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCC
LHTDLVAHFHEKMVERGGRFFVMYGQTEATARIAILPHDRLPEKLGSAGVAIPGGRFHIE
HHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCHHCHHHHCCCCEECCCCEEEEC
SDGRESGELIYTGPNVMMGYATCREDLALGDVLQGRLATGDLARLDSEGFVTIEGRMKRD
CCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCC
AKLFGLRINLDEIESMLRAHGPTAVISGPDKLLIYCEHGDEAAFARYRTDLAAKLKVNHR
CEEEEEEECHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHEEEEECHH
ALEFFRLERLPVNGSGKIDYQALAGAR
HHHHHHHEECCCCCCCCEEHHHCCCCC
>Mature Secondary Structure
MFLQDASIPESNLALFDAAQARWYSYGELWSEVARTAESLKGPKALAFCFCRNNIASVVW
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEHHCCHHHHHH
YLAAVEAGHSVALLDDGLTPEFKTRLIELYDPELIQEAGQHEWRRTNAAGGTIASELAVL
HHHHHHCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHCHHHHHHCCCCCCHHHHHHHHH
LSTSGSTGSPKFVRLTAENVSANARSISEALAIGAADRAISSLPMHYSYGLSVLNTHLLR
HCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHC
GASMVLTNDGLMSPNFWKLFREQECTSFAGVPYSYQMLRRLGIDGLNVPSLRTMTQAGGK
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCC
LHTDLVAHFHEKMVERGGRFFVMYGQTEATARIAILPHDRLPEKLGSAGVAIPGGRFHIE
HHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCHHCHHHHCCCCEECCCCEEEEC
SDGRESGELIYTGPNVMMGYATCREDLALGDVLQGRLATGDLARLDSEGFVTIEGRMKRD
CCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCC
AKLFGLRINLDEIESMLRAHGPTAVISGPDKLLIYCEHGDEAAFARYRTDLAAKLKVNHR
CEEEEEEECHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHEEEEECHH
ALEFFRLERLPVNGSGKIDYQALAGAR
HHHHHHHEECCCCCCCCEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]