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The map label for this gene is cofH [H]

Identifier: 116620431

GI number: 116620431

Start: 1618968

End: 1620086

Strand: Reverse

Name: cofH [H]

Synonym: Acid_1309

Alternate gene names: 116620431

Gene position: 1620086-1618968 (Counterclockwise)

Preceding gene: 116620432

Following gene: 116620428

Centisome position: 16.26

GC content: 61.93

Gene sequence:

>1119_bases
ATGCAAGTCGTATTCGATGATCCCCTGTTGACCCCGATCCGCGCCAAAGTCGAAGCCGGCGAACGCCTGTCTTACGACGA
CGGAGTGACGCTATACCGCAGCAACGACATCCTGGCGATCGGCTATATGGCCAACCTGGTGCGCGAGCGGTTGCACGGAT
CGAAGACCTACTTCAACGTCAACCGCCACATCAACCCGACCGATGTCTGCGTGGCCAGCTGCCGGCTGTGCGCGTTCGGC
AAGCGCGTGCGCGACCCGAAGGCGTACACGATGTCGCTGGAGGAGGTCTGGCACAAGGCCGGCGAAGGCTGGAGCGAAGC
GGTCACGGAGTTCCACATCGTCGGCGGGCTGCATCCCGAGTTGACGCTCGATTGGTTCTGCGAAATGCTGCGCGGGCTCA
AGCAGCGCTTTCCGCAGGTACACCTGAAGGCCTTCACGATGGTGGAGATCGCGTACCTGGCGAAGCGGCAGAAGATTTCC
ATCCGCGAGACTTTGGAGCGGCTGCGCGACGCCGGCATGGATTCGCTGCCCGGCGGCGGCGCGGAGATCTTTAACGAGCG
CGTGCGGCGAATCATCTGCGACCACAAGATCGATGGTAACGAGTGGCTGGAGACGGCCAGGATCGCGCATCAACTGGGCC
TGCGCTCCAACTGCACGATGCTGTACGGCCACCTGGAAAACGACGAAGATCGCGCCGACCACCTTGTGAGGCTGCGGACG
CTGCAGGACGAGACCAACGGCTTCGTCACCTATATTCCGCTGGCCTTCCATCCCGACAATACGCCGCTGCAGCACATCCC
GAAGACGACGGGCTTCAACGACATCAAGAATATCGCGGTGGCGCGGCTGATGCTGGACAACATCCCGCACATCAAGGCGT
ACTGGGTGATGATGACACCTCGAATCGCGCAGATCGCACTAAAGTTCGGCGCCGATGATCTGGACGGCACGATCGTGGAA
GAACGCATCTATCACGATGCGGGCGCGACTACGCAGCAGGGGCTGCGGCGCAACGAACTACTCGCACTGATTCGGAAGGC
GGGACGGGAGCCGATCGAGCGCGACACGCTGTACCGGCCGGTGACACGGACGGAATCCACCTTCACCGTGCTGGTGTAG

Upstream 100 bases:

>100_bases
TCAACGCGGCCTTCTTCACCATGAACGGCTACGTGAGCGTGTTATTCTTTATTTTCTGGGCCGCTGATATTTTCCTCCTC
AAACCGGGTGCTTGAAAACC

Downstream 100 bases:

>100_bases
CTCGGGTCTACTTGGAATCCAGAATAAACGTGGGGACGATGCCGGCGGCCGACGAGAAGCCGGTTTTGCCCATCGAGAGC
CAGCTGGAGCAGCCCTGCAC

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MQVVFDDPLLTPIRAKVEAGERLSYDDGVTLYRSNDILAIGYMANLVRERLHGSKTYFNVNRHINPTDVCVASCRLCAFG
KRVRDPKAYTMSLEEVWHKAGEGWSEAVTEFHIVGGLHPELTLDWFCEMLRGLKQRFPQVHLKAFTMVEIAYLAKRQKIS
IRETLERLRDAGMDSLPGGGAEIFNERVRRIICDHKIDGNEWLETARIAHQLGLRSNCTMLYGHLENDEDRADHLVRLRT
LQDETNGFVTYIPLAFHPDNTPLQHIPKTTGFNDIKNIAVARLMLDNIPHIKAYWVMMTPRIAQIALKFGADDLDGTIVE
ERIYHDAGATTQQGLRRNELLALIRKAGREPIERDTLYRPVTRTESTFTVLV

Sequences:

>Translated_372_residues
MQVVFDDPLLTPIRAKVEAGERLSYDDGVTLYRSNDILAIGYMANLVRERLHGSKTYFNVNRHINPTDVCVASCRLCAFG
KRVRDPKAYTMSLEEVWHKAGEGWSEAVTEFHIVGGLHPELTLDWFCEMLRGLKQRFPQVHLKAFTMVEIAYLAKRQKIS
IRETLERLRDAGMDSLPGGGAEIFNERVRRIICDHKIDGNEWLETARIAHQLGLRSNCTMLYGHLENDEDRADHLVRLRT
LQDETNGFVTYIPLAFHPDNTPLQHIPKTTGFNDIKNIAVARLMLDNIPHIKAYWVMMTPRIAQIALKFGADDLDGTIVE
ERIYHDAGATTQQGLRRNELLALIRKAGREPIERDTLYRPVTRTESTFTVLV
>Mature_372_residues
MQVVFDDPLLTPIRAKVEAGERLSYDDGVTLYRSNDILAIGYMANLVRERLHGSKTYFNVNRHINPTDVCVASCRLCAFG
KRVRDPKAYTMSLEEVWHKAGEGWSEAVTEFHIVGGLHPELTLDWFCEMLRGLKQRFPQVHLKAFTMVEIAYLAKRQKIS
IRETLERLRDAGMDSLPGGGAEIFNERVRRIICDHKIDGNEWLETARIAHQLGLRSNCTMLYGHLENDEDRADHLVRLRT
LQDETNGFVTYIPLAFHPDNTPLQHIPKTTGFNDIKNIAVARLMLDNIPHIKAYWVMMTPRIAQIALKFGADDLDGTIVE
ERIYHDAGATTQQGLRRNELLALIRKAGREPIERDTLYRPVTRTESTFTVLV

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42535; Mature: 42535

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVVFDDPLLTPIRAKVEAGERLSYDDGVTLYRSNDILAIGYMANLVRERLHGSKTYFNV
CCEEECCCCCCHHHHHHCCCCCCCCCCCEEEEECCCEEEHHHHHHHHHHHHCCCCEEEEE
NRHINPTDVCVASCRLCAFGKRVRDPKAYTMSLEEVWHKAGEGWSEAVTEFHIVGGLHPE
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCC
LTLDWFCEMLRGLKQRFPQVHLKAFTMVEIAYLAKRQKISIRETLERLRDAGMDSLPGGG
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
AEIFNERVRRIICDHKIDGNEWLETARIAHQLGLRSNCTMLYGHLENDEDRADHLVRLRT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
LQDETNGFVTYIPLAFHPDNTPLQHIPKTTGFNDIKNIAVARLMLDNIPHIKAYWVMMTP
HHHCCCCEEEEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECC
RIAQIALKFGADDLDGTIVEERIYHDAGATTQQGLRRNELLALIRKAGREPIERDTLYRP
HHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
VTRTESTFTVLV
CCCCCCEEEEEC
>Mature Secondary Structure
MQVVFDDPLLTPIRAKVEAGERLSYDDGVTLYRSNDILAIGYMANLVRERLHGSKTYFNV
CCEEECCCCCCHHHHHHCCCCCCCCCCCEEEEECCCEEEHHHHHHHHHHHHCCCCEEEEE
NRHINPTDVCVASCRLCAFGKRVRDPKAYTMSLEEVWHKAGEGWSEAVTEFHIVGGLHPE
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCC
LTLDWFCEMLRGLKQRFPQVHLKAFTMVEIAYLAKRQKISIRETLERLRDAGMDSLPGGG
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
AEIFNERVRRIICDHKIDGNEWLETARIAHQLGLRSNCTMLYGHLENDEDRADHLVRLRT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
LQDETNGFVTYIPLAFHPDNTPLQHIPKTTGFNDIKNIAVARLMLDNIPHIKAYWVMMTP
HHHCCCCEEEEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECC
RIAQIALKFGADDLDGTIVEERIYHDAGATTQQGLRRNELLALIRKAGREPIERDTLYRP
HHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
VTRTESTFTVLV
CCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]