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The map label for this gene is miaA

Identifier: 116620420

GI number: 116620420

Start: 1597613

End: 1598536

Strand: Reverse

Name: miaA

Synonym: Acid_1297

Alternate gene names: 116620420

Gene position: 1598536-1597613 (Counterclockwise)

Preceding gene: 116620422

Following gene: 116620419

Centisome position: 16.04

GC content: 61.15

Gene sequence:

>924_bases
GTGCCATTGGTTGCAGTCGCCGGTCCGACCGGGTCCGGAAAGAGCGAATTGGCCCTTCTGATTGCCGAAAAATTCCATGG
CGAAATCGTCAACTGCGATTCGTTGCAGGTATACCGCCACTTCGATATCGGCACGGCCAAACTGCCACTGGGGGAGCGCC
GAGGTATCCCCCATCACCTGATTGACATCATCGACCCAAACGAGCTATTTACGGCGGGGGAATACGCACGCATCGCACGC
AACACCATTGCCGATATTTCGGCGCGCGGCCGGCTCCCGATCCTGGCCGGAGGAACTGGTTTCTACCTGCGCGCTCTACT
GGATGGACTCTTCGAGGGGCCAGCCCGGGACCAGCCATTGCGCGACCGCCTGACCGCGCGTGAGGCACGGCGTGCGGGAT
CGCTGCACCGCATCCTAAGAAGGTTGGATTCCGTTTCGGCCGCAAAGATTCACGCAAACGACGTTCCGAAGGTGACCCGG
GCGCTCGAGGTCTGCCTGTTGACGCAGCGTCCGGTGAGTGAGCTTTTCGCGCGCGGCCGAGATTCGCTGCGGGGCTATCG
CACGCTCAAGTTGGGGCTGCTGCCGGATCGCGAGGTGCTGTATCCGCGGCTTGACGCGCGCTGCGCCTGGATGTTTGAGA
ACGGACTGGTGGACGAAGTGCGCGCGATTCTGGCACTGGGCTTCGCGGCGGAGTGCAAGCCTTTCGAATCGCACGGCTAC
AAGCAGGCGCTGCAACACATCCGCGGTGAATTGAATTTGCGTGAGGCGATTTTCTACGCGCAGCGCAATACGCGCAACTA
TGCGAAGCGTCAAATCACGTGGTTCCGACGCGAACCCGAGCTGGTGTGGCTGAAGGATTTCGGCAACGCGCCTGAGATCC
GCGAGACGGCCATGGATCGCGTCGCAAAATTCCTGGGGCAATAA

Upstream 100 bases:

>100_bases
CCTCGCGGCACCCCCGCATCGGGTCGAAAAACTTCTTTTTATTGTATAACGGGGTTTGACCGCAAATACCCCATCCCCTA
CAGACGCAGACGTGACCGAT

Downstream 100 bases:

>100_bases
GCGCACGATCAATTGCAAGAGCGGCGGAACGTTTTTCGATTTCGTTCGTATCATCTTCTGTAAGCACAAGCCACACAGAG
GCTTTGTAAAAAGCAGACAC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPLVAVAGPTGSGKSELALLIAEKFHGEIVNCDSLQVYRHFDIGTAKLPLGERRGIPHHLIDIIDPNELFTAGEYARIAR
NTIADISARGRLPILAGGTGFYLRALLDGLFEGPARDQPLRDRLTAREARRAGSLHRILRRLDSVSAAKIHANDVPKVTR
ALEVCLLTQRPVSELFARGRDSLRGYRTLKLGLLPDREVLYPRLDARCAWMFENGLVDEVRAILALGFAAECKPFESHGY
KQALQHIRGELNLREAIFYAQRNTRNYAKRQITWFRREPELVWLKDFGNAPEIRETAMDRVAKFLGQ

Sequences:

>Translated_307_residues
MPLVAVAGPTGSGKSELALLIAEKFHGEIVNCDSLQVYRHFDIGTAKLPLGERRGIPHHLIDIIDPNELFTAGEYARIAR
NTIADISARGRLPILAGGTGFYLRALLDGLFEGPARDQPLRDRLTAREARRAGSLHRILRRLDSVSAAKIHANDVPKVTR
ALEVCLLTQRPVSELFARGRDSLRGYRTLKLGLLPDREVLYPRLDARCAWMFENGLVDEVRAILALGFAAECKPFESHGY
KQALQHIRGELNLREAIFYAQRNTRNYAKRQITWFRREPELVWLKDFGNAPEIRETAMDRVAKFLGQ
>Mature_306_residues
PLVAVAGPTGSGKSELALLIAEKFHGEIVNCDSLQVYRHFDIGTAKLPLGERRGIPHHLIDIIDPNELFTAGEYARIARN
TIADISARGRLPILAGGTGFYLRALLDGLFEGPARDQPLRDRLTAREARRAGSLHRILRRLDSVSAAKIHANDVPKVTRA
LEVCLLTQRPVSELFARGRDSLRGYRTLKLGLLPDREVLYPRLDARCAWMFENGLVDEVRAILALGFAAECKPFESHGYK
QALQHIRGELNLREAIFYAQRNTRNYAKRQITWFRREPELVWLKDFGNAPEIRETAMDRVAKFLGQ

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=308, Percent_Identity=30.8441558441558, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=36.6013071895425, Blast_Score=185, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI25144712, Length=266, Percent_Identity=29.3233082706767, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24649830, Length=269, Percent_Identity=33.0855018587361, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_SOLUE (Q029I5)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_822576.1
- ProteinModelPortal:   Q029I5
- SMR:   Q029I5
- STRING:   Q029I5
- GeneID:   4430529
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_1297
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q029I5
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34614; Mature: 34483

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLVAVAGPTGSGKSELALLIAEKFHGEIVNCDSLQVYRHFDIGTAKLPLGERRGIPHHL
CCEEEEECCCCCCHHHHHHHHHHHHCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCHHH
IDIIDPNELFTAGEYARIARNTIADISARGRLPILAGGTGFYLRALLDGLFEGPARDQPL
HEEECCHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCH
RDRLTAREARRAGSLHRILRRLDSVSAAKIHANDVPKVTRALEVCLLTQRPVSELFARGR
HHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCH
DSLRGYRTLKLGLLPDREVLYPRLDARCAWMFENGLVDEVRAILALGFAAECKPFESHGY
HHHCCEEEEEEECCCCHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCHHCCCCHHHCCH
KQALQHIRGELNLREAIFYAQRNTRNYAKRQITWFRREPELVWLKDFGNAPEIRETAMDR
HHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHH
VAKFLGQ
HHHHHCC
>Mature Secondary Structure 
PLVAVAGPTGSGKSELALLIAEKFHGEIVNCDSLQVYRHFDIGTAKLPLGERRGIPHHL
CEEEEECCCCCCHHHHHHHHHHHHCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCHHH
IDIIDPNELFTAGEYARIARNTIADISARGRLPILAGGTGFYLRALLDGLFEGPARDQPL
HEEECCHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCH
RDRLTAREARRAGSLHRILRRLDSVSAAKIHANDVPKVTRALEVCLLTQRPVSELFARGR
HHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCH
DSLRGYRTLKLGLLPDREVLYPRLDARCAWMFENGLVDEVRAILALGFAAECKPFESHGY
HHHCCEEEEEEECCCCHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCHHCCCCHHHCCH
KQALQHIRGELNLREAIFYAQRNTRNYAKRQITWFRREPELVWLKDFGNAPEIRETAMDR
HHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHH
VAKFLGQ
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA