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The map label for this gene is ispH [H]

Identifier: 116620382

GI number: 116620382

Start: 1557356

End: 1558327

Strand: Direct

Name: ispH [H]

Synonym: Acid_1259

Alternate gene names: 116620382

Gene position: 1557356-1558327 (Clockwise)

Preceding gene: 116620380

Following gene: 116620383

Centisome position: 15.63

GC content: 59.57

Gene sequence:

>972_bases
ATGCCTCTTAAGCCTTTCGCACCAAATTTCGGCGCGGAGAAAAAGATTTTTCTGCTGAAGCCGCGTGGCTTCTGCGCCGG
GGTCGTCAGGGCGATCGATGTGGTCAAGATTGCTCTTGATCTCTACGGGCCCCCGGTCTACGTGCGGAAGGAAATTGTCC
ACAACAAACATGTGGTGGAGGAACTGCGCGAAGCCGGCGCGGTTTTCGTCGAGGAACTCGACGAGGTGCCCTCGGGCGCG
CGCGCTATTTTCAGCGCGCACGGAGTATCCCCGGCGGTACGCGCCCAGGCGAAGGAGCGCAATCTGGAGATCATCGACGC
CACCTGTCCGCTGGTGACGAAGGTGCATCTGGAAGCGGTGCGGTTCGCGCGCGATGGATTCAGCATCGTGTTGATCGGCC
ACAAGGATCACGATGAAGTGATCGGCACGCTGGGCGAGGTGCCGGATCGCAGCTACCTGGTGGAAACTACGGATGACGTG
GACAACCTGGTGCTGCCCGACCCGACGCGCGTCCGGTACCTGACGCAAACCACGCTGAGCCTGGATGAGACGCGCGACAT
TGTAGATCGGCTCAAACAGCGCTTCCCGATGATCGAAGGACCCCATTCCCAGGACATCTGCTATGCCACCGAAAACCGGC
AGATGGCGGTAAAGGCGATTTCGACAAATGTGGACCTCCTGCTGGTGGTGGGCTCGGAGAACAGTTCCAACTCCAAGCGC
CTCGTGGAAGTGGGCGACAATTTCGGCGTTCGTTCCCATCTGGTGAACGATCGCGGCGATGTGGATGCGAGCTGGCTTGA
CGGCGTCAAGAACGTTGGCGTCACCGCGGGAGCTTCGGCGCCCGAACATCTGGTGCAGGAGCTGATCGAGTTTCTGCGCG
AATGGGGCTTTGAAGGCCTGGAAGAAATCGAACTGGTGGATGAAGACGTACGATTCTCCCTGCCCGCGGAGTTGACGATC
TCGATCCAATGA

Upstream 100 bases:

>100_bases
TCTGTCAAGCAGATTTCGGTCCACTCGATCATGCCCCCAGTTTGTCATACGACCTGCGGCGTTTGTCTTCCACCTGTTAT
AATCGATCTCAATCACTTCT

Downstream 100 bases:

>100_bases
GCGCACCGTCACACGTCGGCAATACGTTGGAGCACGCGGCCGAACTGGCTACCCGCAAGGCGATGGCCTACCTGACCTGC
CTGCAGGAGCGCGACGGTCA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MPLKPFAPNFGAEKKIFLLKPRGFCAGVVRAIDVVKIALDLYGPPVYVRKEIVHNKHVVEELREAGAVFVEELDEVPSGA
RAIFSAHGVSPAVRAQAKERNLEIIDATCPLVTKVHLEAVRFARDGFSIVLIGHKDHDEVIGTLGEVPDRSYLVETTDDV
DNLVLPDPTRVRYLTQTTLSLDETRDIVDRLKQRFPMIEGPHSQDICYATENRQMAVKAISTNVDLLLVVGSENSSNSKR
LVEVGDNFGVRSHLVNDRGDVDASWLDGVKNVGVTAGASAPEHLVQELIEFLREWGFEGLEEIELVDEDVRFSLPAELTI
SIQ

Sequences:

>Translated_323_residues
MPLKPFAPNFGAEKKIFLLKPRGFCAGVVRAIDVVKIALDLYGPPVYVRKEIVHNKHVVEELREAGAVFVEELDEVPSGA
RAIFSAHGVSPAVRAQAKERNLEIIDATCPLVTKVHLEAVRFARDGFSIVLIGHKDHDEVIGTLGEVPDRSYLVETTDDV
DNLVLPDPTRVRYLTQTTLSLDETRDIVDRLKQRFPMIEGPHSQDICYATENRQMAVKAISTNVDLLLVVGSENSSNSKR
LVEVGDNFGVRSHLVNDRGDVDASWLDGVKNVGVTAGASAPEHLVQELIEFLREWGFEGLEEIELVDEDVRFSLPAELTI
SIQ
>Mature_322_residues
PLKPFAPNFGAEKKIFLLKPRGFCAGVVRAIDVVKIALDLYGPPVYVRKEIVHNKHVVEELREAGAVFVEELDEVPSGAR
AIFSAHGVSPAVRAQAKERNLEIIDATCPLVTKVHLEAVRFARDGFSIVLIGHKDHDEVIGTLGEVPDRSYLVETTDDVD
NLVLPDPTRVRYLTQTTLSLDETRDIVDRLKQRFPMIEGPHSQDICYATENRQMAVKAISTNVDLLLVVGSENSSNSKRL
VEVGDNFGVRSHLVNDRGDVDASWLDGVKNVGVTAGASAPEHLVQELIEFLREWGFEGLEEIELVDEDVRFSLPAELTIS
IQ

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=312, Percent_Identity=51.2820512820513, Blast_Score=322, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35767; Mature: 35636

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKPFAPNFGAEKKIFLLKPRGFCAGVVRAIDVVKIALDLYGPPVYVRKEIVHNKHVVE
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ELREAGAVFVEELDEVPSGARAIFSAHGVSPAVRAQAKERNLEIIDATCPLVTKVHLEAV
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
RFARDGFSIVLIGHKDHDEVIGTLGEVPDRSYLVETTDDVDNLVLPDPTRVRYLTQTTLS
HHHCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEEEEHHHHC
LDETRDIVDRLKQRFPMIEGPHSQDICYATENRQMAVKAISTNVDLLLVVGSENSSNSKR
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEEEECCCEEEEEECCCCCCCCCE
LVEVGDNFGVRSHLVNDRGDVDASWLDGVKNVGVTAGASAPEHLVQELIEFLREWGFEGL
EEEECCCCCHHHHHCCCCCCCCHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHCCCCH
EEIELVDEDVRFSLPAELTISIQ
HHHHHHCCCCEEECCCEEEEEEC
>Mature Secondary Structure 
PLKPFAPNFGAEKKIFLLKPRGFCAGVVRAIDVVKIALDLYGPPVYVRKEIVHNKHVVE
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ELREAGAVFVEELDEVPSGARAIFSAHGVSPAVRAQAKERNLEIIDATCPLVTKVHLEAV
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
RFARDGFSIVLIGHKDHDEVIGTLGEVPDRSYLVETTDDVDNLVLPDPTRVRYLTQTTLS
HHHCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEEEEHHHHC
LDETRDIVDRLKQRFPMIEGPHSQDICYATENRQMAVKAISTNVDLLLVVGSENSSNSKR
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEEEECCCEEEEEECCCCCCCCCE
LVEVGDNFGVRSHLVNDRGDVDASWLDGVKNVGVTAGASAPEHLVQELIEFLREWGFEGL
EEEECCCCCHHHHHCCCCCCCCHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHCCCCH
EEIELVDEDVRFSLPAELTISIQ
HHHHHHCCCCEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA