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The map label for this gene is dctA [H]

Identifier: 116620240

GI number: 116620240

Start: 1373956

End: 1375149

Strand: Direct

Name: dctA [H]

Synonym: Acid_1117

Alternate gene names: 116620240

Gene position: 1373956-1375149 (Clockwise)

Preceding gene: 116620239

Following gene: 116620241

Centisome position: 13.79

GC content: 62.9

Gene sequence:

>1194_bases
ATGAAAAGAATTTCGCTGACGGCATGGATCTTTATCGGCATGGCCGCCGGTGTGGCCCTCGGTGTCCTTGCGCCCTCATT
CGCCGCGCAACTCGGCCTCCTAAGCACGATTTTCCTCCGCCTCATCCGGTCCATCATCGCGCCCCTCATCTTCGCCACCC
TGGTCGCCGGCATCGCCGGCGGCGGCGATATCAAACACATGGGCCGCATCGGCGGCAAGGCGATTTTGTACTTTGAAATC
GTCACCACCTTCGCACTCTTCCTCGGCCTCGGCATCGTCAACCTCGTCCGCCCCGGCGACGGAGTGCCGATCGCGCGCAC
TGCCGCCGATAAAGTCGCCGCCGGTCCGCCGGCATCGCTCGCCACCATCGTGGAGCACACCTTCCCGGCAAGCATCATCG
ACGCCATGGCACGCAACGACGTTCTACAGGTGGTCGTCTTCGCCTTCCTCTTCGGCGCCGCCTGTGCCGCCATTGGCGGA
AAGGCCGAGCCCGTGGTCTCGTTCTGTTCGTCGCTTGCCGAGGTGATGTTCCGCTATACGCGCTACGTCATGTATCTCGC
GCCGCTCGGCGTCGGCGCCGCGCTCGCCGTCACCGTCGGCGCCAAAGGCATCGGCGTACTCTTCGGACTCGGCAAGTTGA
TCCTCACCATGTACAGTGCGCTGATCCTGTTCGTGGTCATCGTGCTTGGCGCCGTGGTGAGCATCACGCGTATCCCCATG
CGGTCGTTTTACCAGGCCGTGCGCGAGCCTTTCATGATCGCCTTTTCCACTGCCAGCAGTGAAGCCGCTCTGCCTCTGGC
GCTCGAGAATATGGAAAAGTTCGGCGTGCCCAAACACATCGTCGGCTTCGTGCTTCCCACCGGCTACAGCTTCAACCTGG
ATGGCACCACGCTCTACCTCTCTATGGCGTCGATGTTTGTCGCCCAGGCCGCCGGCGTTCACATGACCTTCGGCGAGCAG
TTGATTATGATGCTGACGCTCATGCTCACCAGCAAGGGAGTCGCCGGAGTGCCGCGCTCCGCGCTGGTGATCCTCGCCGG
TACCCTGACCACCTTCCACCTCCCGGTGGAAGGCTTCGCTCTTCTCCTTGGTGTCGATGCCGTGCTCGATATGGCGCGCA
CTTCCGTCAACGTCCTCGGCAACTGCCTGGCCAGCGCCGTGGTGGCTCGCTGGGAAGGCTTCAAGGTCGGCTGA

Upstream 100 bases:

>100_bases
CCGTAGTCTGCCATAGCTCGCACCATTCGCTGCTGCTGATCCGTCCGCCGCGCATGATGTTCTTCGATGTGCTGCGCCAG
AAACTCAAGTGGGGAGAACG

Downstream 100 bases:

>100_bases
CAACGACGACTGTTTTATACTTGAGCGAGCATGTTCCGAATAGCAGTTCTGCTTGTCTGTGCCCTTCCGTTGTCCGCCTT
TCAAGGAGCCGATTCCACGG

Product: sodium:dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKRISLTAWIFIGMAAGVALGVLAPSFAAQLGLLSTIFLRLIRSIIAPLIFATLVAGIAGGGDIKHMGRIGGKAILYFEI
VTTFALFLGLGIVNLVRPGDGVPIARTAADKVAAGPPASLATIVEHTFPASIIDAMARNDVLQVVVFAFLFGAACAAIGG
KAEPVVSFCSSLAEVMFRYTRYVMYLAPLGVGAALAVTVGAKGIGVLFGLGKLILTMYSALILFVVIVLGAVVSITRIPM
RSFYQAVREPFMIAFSTASSEAALPLALENMEKFGVPKHIVGFVLPTGYSFNLDGTTLYLSMASMFVAQAAGVHMTFGEQ
LIMMLTLMLTSKGVAGVPRSALVILAGTLTTFHLPVEGFALLLGVDAVLDMARTSVNVLGNCLASAVVARWEGFKVG

Sequences:

>Translated_397_residues
MKRISLTAWIFIGMAAGVALGVLAPSFAAQLGLLSTIFLRLIRSIIAPLIFATLVAGIAGGGDIKHMGRIGGKAILYFEI
VTTFALFLGLGIVNLVRPGDGVPIARTAADKVAAGPPASLATIVEHTFPASIIDAMARNDVLQVVVFAFLFGAACAAIGG
KAEPVVSFCSSLAEVMFRYTRYVMYLAPLGVGAALAVTVGAKGIGVLFGLGKLILTMYSALILFVVIVLGAVVSITRIPM
RSFYQAVREPFMIAFSTASSEAALPLALENMEKFGVPKHIVGFVLPTGYSFNLDGTTLYLSMASMFVAQAAGVHMTFGEQ
LIMMLTLMLTSKGVAGVPRSALVILAGTLTTFHLPVEGFALLLGVDAVLDMARTSVNVLGNCLASAVVARWEGFKVG
>Mature_397_residues
MKRISLTAWIFIGMAAGVALGVLAPSFAAQLGLLSTIFLRLIRSIIAPLIFATLVAGIAGGGDIKHMGRIGGKAILYFEI
VTTFALFLGLGIVNLVRPGDGVPIARTAADKVAAGPPASLATIVEHTFPASIIDAMARNDVLQVVVFAFLFGAACAAIGG
KAEPVVSFCSSLAEVMFRYTRYVMYLAPLGVGAALAVTVGAKGIGVLFGLGKLILTMYSALILFVVIVLGAVVSITRIPM
RSFYQAVREPFMIAFSTASSEAALPLALENMEKFGVPKHIVGFVLPTGYSFNLDGTTLYLSMASMFVAQAAGVHMTFGEQ
LIMMLTLMLTSKGVAGVPRSALVILAGTLTTFHLPVEGFALLLGVDAVLDMARTSVNVLGNCLASAVVARWEGFKVG

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI21314632, Length=399, Percent_Identity=29.0726817042606, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI66773030, Length=455, Percent_Identity=26.1538461538462, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI40254478, Length=423, Percent_Identity=28.6052009456265, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI169790839, Length=411, Percent_Identity=27.2506082725061, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI5032093, Length=381, Percent_Identity=29.1338582677165, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI223468564, Length=256, Percent_Identity=32.421875, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI223468566, Length=256, Percent_Identity=32.421875, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4827012, Length=264, Percent_Identity=32.5757575757576, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI194239697, Length=258, Percent_Identity=31.0077519379845, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI262359914, Length=357, Percent_Identity=25.7703081232493, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI301601644, Length=147, Percent_Identity=31.9727891156463, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789947, Length=394, Percent_Identity=43.4010152284264, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1790514, Length=413, Percent_Identity=40.6779661016949, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI1788024, Length=374, Percent_Identity=23.7967914438503, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789473, Length=323, Percent_Identity=20.4334365325077, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=439, Percent_Identity=28.246013667426, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI193206505, Length=435, Percent_Identity=29.4252873563218, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI71983099, Length=429, Percent_Identity=26.8065268065268, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71983106, Length=429, Percent_Identity=26.8065268065268, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71996953, Length=448, Percent_Identity=27.6785714285714, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI193206654, Length=446, Percent_Identity=27.1300448430493, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17541374, Length=411, Percent_Identity=25.7907542579075, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24583025, Length=390, Percent_Identity=27.4358974358974, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI17137668, Length=390, Percent_Identity=27.4358974358974, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24583023, Length=390, Percent_Identity=27.4358974358974, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI281360481, Length=416, Percent_Identity=30.2884615384615, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI281360483, Length=416, Percent_Identity=30.2884615384615, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI17137666, Length=416, Percent_Identity=30.2884615384615, Blast_Score=131, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 41484; Mature: 41484

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA