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The map label for this gene is pckA1 [H]

Identifier: 116620206

GI number: 116620206

Start: 1331295

End: 1332893

Strand: Reverse

Name: pckA1 [H]

Synonym: Acid_1079

Alternate gene names: 116620206

Gene position: 1332893-1331295 (Counterclockwise)

Preceding gene: 116620208

Following gene: 116620205

Centisome position: 13.37

GC content: 63.1

Gene sequence:

>1599_bases
ATGCAGAATCTAGGTATTCTTCCCAGCCGTTACGGTCTCCAGGAACACGGCATTCGCAACGCCAGCTTCGTGTATTGGAA
TCTGGGGACGGCTCAGCTGGTCGAGAAGGCCATCCAGCGCCGCGAGGGCCTGCTGGCTTCCGGCGGGGGACTCGTGGTGC
GCACCGGCGATCAGACCGGCCGTTCGCCGCGCGATAAATTCGTGGTCCGCGACGCCGGCACCGAGAACACCGTGAACTGG
GGCAGCGTAAACCAGCCCTTCCTCCCCGAGCAGTTCGACCGCCTCTACGGAAAACTGCTCGCCTACCTGCAGGCGCGCGA
CCTCTTCGTGCAGGATTGCTACGCCGGCGCCGACCCGCAGTATCAGATTCCGATCCGCGTCATCACCGAGCTCGCCTGGC
ACAACCTTTTCGCGCGCCAGCTCTTCGTGCGCGCCCACCCCGAAAAGACCGATAGCCATGTCCCGCAGTTTACCGTCATC
GATGCGCCGGGCTTCCGCGCCGAAGCGACCGACGACGGCACGAATTCGGACGTTTTCGTGATCATCAATTTCAGCAAGAA
GCTGGTGATCATCGGCGGCACTCAATACGCCGGCGAAATGAAGAAGGCCATCTTCAGCGTGCTGAACTACTACCTGCCCA
CGCAGGACGTGCTTCCCATGCATTGCTCCGCGAACATCGGCACCGATAACGAGACCGCGCTTTTCTTCGGCCTCTCCGGC
ACCGGCAAGACCACACTGTCCGCCGACCCCTCCCGCCGCCTGATTGGCGACGACGAGCATGGCTGGGCGCCCAGCGGCGT
CTTCAATTTCGAAGGCGGCTGCTACGCCAAGTGCATCCGGCTGCGGCACGAAACCGAGCCGCAGATCTGGGACGCGATCC
GCTTCGGCACGGTGCTCGAGAACGTCGCCGTGGATAGCGAGCTCCGCCTGCTCGATTACGACGACACCACCTTCACGGAA
AATACGCGCGCGGCCTACCCGGTCACGTTCATCGAAAACGCGGTCCTGCCCGGACTCGGCGGACATCCCAAGAACATCGT
CTTCCTCACCTGCGATGCCTTCGGCGTCCTGCCTCCGCTCTCCCGCCTTACGCCCGACCAGGCGATGTTCCACTTCCTAA
ACGGCTACACCGCCAAAGTCGCGGGCACCGAACGCGGACTCGGCAAAGAGCCCGAAGCCACCTTCAGCTCCTGCTTTGGA
TCGCCCTTCCTGCCCCGCAATCCTGAGGTTTACGCGGAGATGCTCGGCAGGAAGATGACCCAGCAAGGCGCTGCCTGCTG
GCTGATCAACACCGGGTGGAGCGGCGGCCCTTACGGCGTCGGCAATCGCATGAGCCTCCCGATCACTCGCGCACTGGTTA
CGGAAGCGCTCAGCGGCGCGCTGGCCAAGGCCGATTACCGCATCGACCCGGTCTTCAAGGTTCAGGTCCCGGTGGCCTGC
CCCAATGTGGACTCCAAGGCGCTCGATCCGCGCGCCACCTGGAGCGATCCCAAAGCCTACGACAAGGCCGCCGATGACCT
GGCCGCGCGCTTTCGCAAAAACTTCGCCAAATTCGAAAAGGCCGGCGGCATTCTGAAGTGCGAAGAAGTACTGGCGTAA

Upstream 100 bases:

>100_bases
TCACCGATGCAGGCGAGCATTTACACGTTTGAATCCAAGCGTTTAGCTTGGCCGCTAAATTGCACCGGAGACTCTGAATA
TTTTTCAGGAGAAATCATAA

Downstream 100 bases:

>100_bases
TTCCTACCCGCCATTCCGGGCTGCCCGTCACACCGCGGGCAGCCCTTTCCGCAGAGCCCCCACCGCTCGCATCCGGACTC
ATGATAGGATGATGGCCAAT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase 1; PEPCK; Phosphoenolpyruvate carboxylase 1 [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MQNLGILPSRYGLQEHGIRNASFVYWNLGTAQLVEKAIQRREGLLASGGGLVVRTGDQTGRSPRDKFVVRDAGTENTVNW
GSVNQPFLPEQFDRLYGKLLAYLQARDLFVQDCYAGADPQYQIPIRVITELAWHNLFARQLFVRAHPEKTDSHVPQFTVI
DAPGFRAEATDDGTNSDVFVIINFSKKLVIIGGTQYAGEMKKAIFSVLNYYLPTQDVLPMHCSANIGTDNETALFFGLSG
TGKTTLSADPSRRLIGDDEHGWAPSGVFNFEGGCYAKCIRLRHETEPQIWDAIRFGTVLENVAVDSELRLLDYDDTTFTE
NTRAAYPVTFIENAVLPGLGGHPKNIVFLTCDAFGVLPPLSRLTPDQAMFHFLNGYTAKVAGTERGLGKEPEATFSSCFG
SPFLPRNPEVYAEMLGRKMTQQGAACWLINTGWSGGPYGVGNRMSLPITRALVTEALSGALAKADYRIDPVFKVQVPVAC
PNVDSKALDPRATWSDPKAYDKAADDLAARFRKNFAKFEKAGGILKCEEVLA

Sequences:

>Translated_532_residues
MQNLGILPSRYGLQEHGIRNASFVYWNLGTAQLVEKAIQRREGLLASGGGLVVRTGDQTGRSPRDKFVVRDAGTENTVNW
GSVNQPFLPEQFDRLYGKLLAYLQARDLFVQDCYAGADPQYQIPIRVITELAWHNLFARQLFVRAHPEKTDSHVPQFTVI
DAPGFRAEATDDGTNSDVFVIINFSKKLVIIGGTQYAGEMKKAIFSVLNYYLPTQDVLPMHCSANIGTDNETALFFGLSG
TGKTTLSADPSRRLIGDDEHGWAPSGVFNFEGGCYAKCIRLRHETEPQIWDAIRFGTVLENVAVDSELRLLDYDDTTFTE
NTRAAYPVTFIENAVLPGLGGHPKNIVFLTCDAFGVLPPLSRLTPDQAMFHFLNGYTAKVAGTERGLGKEPEATFSSCFG
SPFLPRNPEVYAEMLGRKMTQQGAACWLINTGWSGGPYGVGNRMSLPITRALVTEALSGALAKADYRIDPVFKVQVPVAC
PNVDSKALDPRATWSDPKAYDKAADDLAARFRKNFAKFEKAGGILKCEEVLA
>Mature_532_residues
MQNLGILPSRYGLQEHGIRNASFVYWNLGTAQLVEKAIQRREGLLASGGGLVVRTGDQTGRSPRDKFVVRDAGTENTVNW
GSVNQPFLPEQFDRLYGKLLAYLQARDLFVQDCYAGADPQYQIPIRVITELAWHNLFARQLFVRAHPEKTDSHVPQFTVI
DAPGFRAEATDDGTNSDVFVIINFSKKLVIIGGTQYAGEMKKAIFSVLNYYLPTQDVLPMHCSANIGTDNETALFFGLSG
TGKTTLSADPSRRLIGDDEHGWAPSGVFNFEGGCYAKCIRLRHETEPQIWDAIRFGTVLENVAVDSELRLLDYDDTTFTE
NTRAAYPVTFIENAVLPGLGGHPKNIVFLTCDAFGVLPPLSRLTPDQAMFHFLNGYTAKVAGTERGLGKEPEATFSSCFG
SPFLPRNPEVYAEMLGRKMTQQGAACWLINTGWSGGPYGVGNRMSLPITRALVTEALSGALAKADYRIDPVFKVQVPVAC
PNVDSKALDPRATWSDPKAYDKAADDLAARFRKNFAKFEKAGGILKCEEVLA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=529, Percent_Identity=44.9905482041588, Blast_Score=458, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322950, Length=501, Percent_Identity=47.1057884231537, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 58542; Mature: 58542

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLGILPSRYGLQEHGIRNASFVYWNLGTAQLVEKAIQRREGLLASGGGLVVRTGDQTG
CCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCEECCCCEEEECCCCCC
RSPRDKFVVRDAGTENTVNWGSVNQPFLPEQFDRLYGKLLAYLQARDLFVQDCYAGADPQ
CCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YQIPIRVITELAWHNLFARQLFVRAHPEKTDSHVPQFTVIDAPGFRAEATDDGTNSDVFV
EECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEE
IINFSKKLVIIGGTQYAGEMKKAIFSVLNYYLPTQDVLPMHCSANIGTDNETALFFGLSG
EEECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECC
TGKTTLSADPSRRLIGDDEHGWAPSGVFNFEGGCYAKCIRLRHETEPQIWDAIRFGTVLE
CCCEEECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
NVAVDSELRLLDYDDTTFTENTRAAYPVTFIENAVLPGLGGHPKNIVFLTCDAFGVLPPL
HHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCH
SRLTPDQAMFHFLNGYTAKVAGTERGLGKEPEATFSSCFGSPFLPRNPEVYAEMLGRKMT
HHCCHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHH
QQGAACWLINTGWSGGPYGVGNRMSLPITRALVTEALSGALAKADYRIDPVFKVQVPVAC
HCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEEEC
PNVDSKALDPRATWSDPKAYDKAADDLAARFRKNFAKFEKAGGILKCEEVLA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCC
>Mature Secondary Structure
MQNLGILPSRYGLQEHGIRNASFVYWNLGTAQLVEKAIQRREGLLASGGGLVVRTGDQTG
CCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCEECCCCEEEECCCCCC
RSPRDKFVVRDAGTENTVNWGSVNQPFLPEQFDRLYGKLLAYLQARDLFVQDCYAGADPQ
CCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YQIPIRVITELAWHNLFARQLFVRAHPEKTDSHVPQFTVIDAPGFRAEATDDGTNSDVFV
EECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEE
IINFSKKLVIIGGTQYAGEMKKAIFSVLNYYLPTQDVLPMHCSANIGTDNETALFFGLSG
EEECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECC
TGKTTLSADPSRRLIGDDEHGWAPSGVFNFEGGCYAKCIRLRHETEPQIWDAIRFGTVLE
CCCEEECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
NVAVDSELRLLDYDDTTFTENTRAAYPVTFIENAVLPGLGGHPKNIVFLTCDAFGVLPPL
HHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCH
SRLTPDQAMFHFLNGYTAKVAGTERGLGKEPEATFSSCFGSPFLPRNPEVYAEMLGRKMT
HHCCHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHH
QQGAACWLINTGWSGGPYGVGNRMSLPITRALVTEALSGALAKADYRIDPVFKVQVPVAC
HCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEEEC
PNVDSKALDPRATWSDPKAYDKAADDLAARFRKNFAKFEKAGGILKCEEVLA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA