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The map label for this gene is bphF [H]

Identifier: 116620173

GI number: 116620173

Start: 1294522

End: 1295364

Strand: Direct

Name: bphF [H]

Synonym: Acid_1046

Alternate gene names: 116620173

Gene position: 1294522-1295364 (Clockwise)

Preceding gene: 116620171

Following gene: 116620178

Centisome position: 12.99

GC content: 58.24

Gene sequence:

>843_bases
ATGAGCGAATCACTCAGCGGTGCGGAATTTAAGAAACAACTGCGTGCGGGACAGCCGAAGATGGGGCTGTTCGTGAACTC
GCACAGTCCCACGGTCGCCGAACAACTGGCGCACAGCGGCTATGACTGGCTGCTGATCGATACGCAGCACGGCCCGATGG
GCTTTCCCGATCTCTCCGCGATGCTGGCGGGAGTTTCGGGCGGGGGCGCCAAATCGATGGTCCGGGTGGCCGGCTATCAC
GATCGCGGCGGCATTCAGCAGTCTCTCGATATCGGCGCGGATGGCGTGCTGGTGCCATACATCAATACCGCTGAAGAGGC
GCGGCAGGCGGTCAGTTGCACGCGCTATCCAACCGCCGGCACACGCTCGGTTTATTTTCCGCAGCGGAGTATGAACAAGG
CCGGACTGTTGGGTTATGCCGGAGCTGCGAACGACAACGTGATTGTCGCGCTGCAGGTGGAGACCGCATCTTGCATCGAG
AATATCGACGAGATTGCGGCAGTGCAGGGAGTGGACATTCTGTTCCTTGGGCAGAACGATCTCTGCATGTCGATGGGACT
TTACGAGAAGTACGAGTTCCCGCACATGTACACGTCGCCCGAATTGCAGGCGGCGACCGACAAGCTGATTGCGGCCGCCA
AACGGAATAACGTCATCCTGGGGCTGTTCTTATTCGGAACGTCGCGGGTCGCGGAGTTTCTGGACAAGGATTTCACGTTT
ATCAGCATTGGCAACGATTTGCACCACATCCTCACGCAGGTCGGCGCCTATGTAAAGGATATGGAATCGATCGCCAAAGG
CAGGGCGAATAGCTGGATGCGACGACCAACGGCACTGTTGTAG

Upstream 100 bases:

>100_bases
GAACGTCGGAGTCTTGGCCAGCGTTCGCAGGCCGTAGCGCATATCCTGAAGCAAAATATCCAGCATCGCCGCCATCCTAA
TCCAAAGTATACTGATCGGA

Downstream 100 bases:

>100_bases
CCGTTCACACACGTAGTCCTCTTTTCATCAGGACCCCACAAACGAAAGAGCGTTGCGAACTCGGCCGAAGGGTAGGCGCT
CCTGTTCGAGCCGGACTTGA

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: HOA [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSESLSGAEFKKQLRAGQPKMGLFVNSHSPTVAEQLAHSGYDWLLIDTQHGPMGFPDLSAMLAGVSGGGAKSMVRVAGYH
DRGGIQQSLDIGADGVLVPYINTAEEARQAVSCTRYPTAGTRSVYFPQRSMNKAGLLGYAGAANDNVIVALQVETASCIE
NIDEIAAVQGVDILFLGQNDLCMSMGLYEKYEFPHMYTSPELQAATDKLIAAAKRNNVILGLFLFGTSRVAEFLDKDFTF
ISIGNDLHHILTQVGAYVKDMESIAKGRANSWMRRPTALL

Sequences:

>Translated_280_residues
MSESLSGAEFKKQLRAGQPKMGLFVNSHSPTVAEQLAHSGYDWLLIDTQHGPMGFPDLSAMLAGVSGGGAKSMVRVAGYH
DRGGIQQSLDIGADGVLVPYINTAEEARQAVSCTRYPTAGTRSVYFPQRSMNKAGLLGYAGAANDNVIVALQVETASCIE
NIDEIAAVQGVDILFLGQNDLCMSMGLYEKYEFPHMYTSPELQAATDKLIAAAKRNNVILGLFLFGTSRVAEFLDKDFTF
ISIGNDLHHILTQVGAYVKDMESIAKGRANSWMRRPTALL
>Mature_279_residues
SESLSGAEFKKQLRAGQPKMGLFVNSHSPTVAEQLAHSGYDWLLIDTQHGPMGFPDLSAMLAGVSGGGAKSMVRVAGYHD
RGGIQQSLDIGADGVLVPYINTAEEARQAVSCTRYPTAGTRSVYFPQRSMNKAGLLGYAGAANDNVIVALQVETASCIEN
IDEIAAVQGVDILFLGQNDLCMSMGLYEKYEFPHMYTSPELQAATDKLIAAAKRNNVILGLFLFGTSRVAEFLDKDFTFI
SIGNDLHHILTQVGAYVKDMESIAKGRANSWMRRPTALL

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-oxovalerate to pyruvate and acetaldehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=239, Percent_Identity=34.7280334728033, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789514, Length=249, Percent_Identity=29.3172690763052, Blast_Score=121, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 30272; Mature: 30141

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESLSGAEFKKQLRAGQPKMGLFVNSHSPTVAEQLAHSGYDWLLIDTQHGPMGFPDLSA
CCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHH
MLAGVSGGGAKSMVRVAGYHDRGGIQQSLDIGADGVLVPYINTAEEARQAVSCTRYPTAG
HHHCCCCCCHHHHHEEECCCCCCCCCHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCC
TRSVYFPQRSMNKAGLLGYAGAANDNVIVALQVETASCIENIDEIAAVQGVDILFLGQND
CCEEECCHHCCCCCCCEEECCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCEEEEECCCC
LCMSMGLYEKYEFPHMYTSPELQAATDKLIAAAKRNNVILGLFLFGTSRVAEFLDKDFTF
CEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCEE
ISIGNDLHHILTQVGAYVKDMESIAKGRANSWMRRPTALL
EEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCC
>Mature Secondary Structure 
SESLSGAEFKKQLRAGQPKMGLFVNSHSPTVAEQLAHSGYDWLLIDTQHGPMGFPDLSA
CCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHH
MLAGVSGGGAKSMVRVAGYHDRGGIQQSLDIGADGVLVPYINTAEEARQAVSCTRYPTAG
HHHCCCCCCHHHHHEEECCCCCCCCCHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCC
TRSVYFPQRSMNKAGLLGYAGAANDNVIVALQVETASCIENIDEIAAVQGVDILFLGQND
CCEEECCHHCCCCCCCEEECCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCEEEEECCCC
LCMSMGLYEKYEFPHMYTSPELQAATDKLIAAAKRNNVILGLFLFGTSRVAEFLDKDFTF
CEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCEE
ISIGNDLHHILTQVGAYVKDMESIAKGRANSWMRRPTALL
EEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10049392 [H]