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The map label for this gene is algU [H]

Identifier: 116620147

GI number: 116620147

Start: 1266183

End: 1266761

Strand: Direct

Name: algU [H]

Synonym: Acid_1020

Alternate gene names: 116620147

Gene position: 1266183-1266761 (Clockwise)

Preceding gene: 116620142

Following gene: 116620148

Centisome position: 12.71

GC content: 54.4

Gene sequence:

>579_bases
ATGCTGGTTGATTTCGAACTGATCACGCGGGCCCAGAAGGGTGACAGCGCGGCGTTCAACGAAATCGTTCTGGTCTACCG
GAAGCGGATTCTGGGGACGATCACCCGTTTGATCGCGCGTCCGGAAGACGTCGAGGATGTCGCGCAGGAAGTCTTTCTGC
GGCTTTATTTTTCCCTCGATCAATTGCGGACGGCAGAGGTCTTTGAACCTTGGTTGTACCGTCTGACGGTCAATGCGGCC
TACGATTACCTGCGCAAGCAGCGGCGTCGCCAGGAGTTCCGCATGTCCGACCTGAGCGAGCAGCAGGTTATGCTGGCCGA
TGCCATGGCAGGCGGGAAAGCCGAACAGGATGTACAGCATCAAAAGAAGGTTCGCGAGTCCGTGGATTCCCTCTTGGGAG
CCGTAAGCGAGGCCGATCGAATTCTTTTGATGTTGAAAGAAGTGGAAGGCCTTTCATTGAAAGAGCTTGAAAAGGTCTAT
AAGGTCAACGAGAATGCTCTTAAGGTCCGTTTGTTCCGTGCCCGGCAAAGGGTGTTAAAAGCCTTCGGGGCGGCAGGAAA
AAAAGAGGAAGCCGGTTAA

Upstream 100 bases:

>100_bases
GCGTTTCCCCGCAAAGCCTTGTAACCTGTCTACTTGCCGGTACGCCTTTCAAATCGAAGAGAATTTTGTTTCCGGGTGCC
GGCACAACCCATGGTGCCGC

Downstream 100 bases:

>100_bases
ACCGGTGGAGTAGAGTTATTATGAGTCTTATGCGCGGCGGCGACGACGAAAAATTGGACGCACTGTTCCGGGCGTACAAC
CAGGCCTGCGGATCTCCCGA

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MLVDFELITRAQKGDSAAFNEIVLVYRKRILGTITRLIARPEDVEDVAQEVFLRLYFSLDQLRTAEVFEPWLYRLTVNAA
YDYLRKQRRRQEFRMSDLSEQQVMLADAMAGGKAEQDVQHQKKVRESVDSLLGAVSEADRILLMLKEVEGLSLKELEKVY
KVNENALKVRLFRARQRVLKAFGAAGKKEEAG

Sequences:

>Translated_192_residues
MLVDFELITRAQKGDSAAFNEIVLVYRKRILGTITRLIARPEDVEDVAQEVFLRLYFSLDQLRTAEVFEPWLYRLTVNAA
YDYLRKQRRRQEFRMSDLSEQQVMLADAMAGGKAEQDVQHQKKVRESVDSLLGAVSEADRILLMLKEVEGLSLKELEKVY
KVNENALKVRLFRARQRVLKAFGAAGKKEEAG
>Mature_192_residues
MLVDFELITRAQKGDSAAFNEIVLVYRKRILGTITRLIARPEDVEDVAQEVFLRLYFSLDQLRTAEVFEPWLYRLTVNAA
YDYLRKQRRRQEFRMSDLSEQQVMLADAMAGGKAEQDVQHQKKVRESVDSLLGAVSEADRILLMLKEVEGLSLKELEKVY
KVNENALKVRLFRARQRVLKAFGAAGKKEEAG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=183, Percent_Identity=31.6939890710383, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22125; Mature: 22125

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVDFELITRAQKGDSAAFNEIVLVYRKRILGTITRLIARPEDVEDVAQEVFLRLYFSLD
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QLRTAEVFEPWLYRLTVNAAYDYLRKQRRRQEFRMSDLSEQQVMLADAMAGGKAEQDVQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHH
QKKVRESVDSLLGAVSEADRILLMLKEVEGLSLKELEKVYKVNENALKVRLFRARQRVLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AFGAAGKKEEAG
HHCCCCCCCCCC
>Mature Secondary Structure
MLVDFELITRAQKGDSAAFNEIVLVYRKRILGTITRLIARPEDVEDVAQEVFLRLYFSLD
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QLRTAEVFEPWLYRLTVNAAYDYLRKQRRRQEFRMSDLSEQQVMLADAMAGGKAEQDVQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHH
QKKVRESVDSLLGAVSEADRILLMLKEVEGLSLKELEKVYKVNENALKVRLFRARQRVLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AFGAAGKKEEAG
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]