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The map label for this gene is gca [H]

Identifier: 116620146

GI number: 116620146

Start: 1264990

End: 1265970

Strand: Reverse

Name: gca [H]

Synonym: Acid_1019

Alternate gene names: 116620146

Gene position: 1265970-1264990 (Counterclockwise)

Preceding gene: 116620150

Following gene: 116620145

Centisome position: 12.7

GC content: 60.45

Gene sequence:

>981_bases
ATGGCCTCCAAATGCGCTCTGCTTACAGGGATTACCGGCCAGGACGGCTCGTATCTTTCGGAGTGGCTCCTTTCCCAGGG
CTACGACGTGCACGGAATTGTACGCCGGGTGGCCCTGGAGGACCCGGACCACCGGCTCAGCCGGTTGAAAGAGGTCCGCG
AACGGCTGCACCTGCACGCGGCCTCGCTGGAAAGCTACGGCAGCATCCACCGGGTGGTTCACGAGGTTCAACCCGATGAA
TGCTATCACCTTGCGGCTCAAAGCTTTGTGAGCTATTCGTTTGACGACGAGTTCTCCACTCTTAATGCCAATATCAACGG
CACACACTACCTGCTCTCGGCGCTGAAGGCGCTGACCCCGGATTGCCGGTTCTACTTCGCGGGCTCGAGCGAAATGTTCG
GGATGGCGGAAACCGTGCCGCAGAACGAGGATACGCGGTTTCATCCGCGCTCTTCTTATGGCATTAGTAAGGTTGCCGGC
TTCGATTTGACGCGCAACTACCGGGAAGCATATGGGATGCATGCTTCCAGCGGAATCCTGTTCAACCACGAGTCGCCACG
CCGCGGCTTCGAGTTCGTGACGCGGAAGATCGCTGCCGGAGTGGCCCGGATCGTAGCGGGCAAAAGCAGGGAATTGCGTC
TGGGGAACCTGGATGCGAAGCGCGATTGGGGACACGCGCGAGAATACGTGACCGCGATGTGGATGATGTTACAACAGACC
ACGCCGGACGACTATGTTGTGGCGACCGGCGACTGCCACTCGGTTCGCGAGTTCGCGGAGCTCGCGTTTTCGCACGTGGG
ACTGGACTACACGAAGTACATCACCACCGATCCGCAACTCTTTCGTCCGGCCGAGGTCAACTTGTTGAAGGGGGATGCCG
CCAAGGCGCAGCGCGTACTCGGCTGGCGCCACCGCGTGACTTTTCCGGAGTTGGTGCGGGAGATGGTGGACGAGGATTGC
CGCGCGCTGGGGATCGGGTAG

Upstream 100 bases:

>100_bases
TGCGTCCGGGAAACGCTGCTCCGTTGACTCTATCGAGCCATATCGGACCGGAATCCGCGACGATCCGTGAGGTCATGTAC
TTGTTACCATTATTCCAGAG

Downstream 100 bases:

>100_bases
AACCGTAAGGGACCGGTCGGGAACTCAGTCTCACTCTGCTAGCCTGAAGGTATATGCAGTTTTCCGATTCCATCTTCGCA
ACACGCTTCGGGATCACACG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MASKCALLTGITGQDGSYLSEWLLSQGYDVHGIVRRVALEDPDHRLSRLKEVRERLHLHAASLESYGSIHRVVHEVQPDE
CYHLAAQSFVSYSFDDEFSTLNANINGTHYLLSALKALTPDCRFYFAGSSEMFGMAETVPQNEDTRFHPRSSYGISKVAG
FDLTRNYREAYGMHASSGILFNHESPRRGFEFVTRKIAAGVARIVAGKSRELRLGNLDAKRDWGHAREYVTAMWMMLQQT
TPDDYVVATGDCHSVREFAELAFSHVGLDYTKYITTDPQLFRPAEVNLLKGDAAKAQRVLGWRHRVTFPELVREMVDEDC
RALGIG

Sequences:

>Translated_326_residues
MASKCALLTGITGQDGSYLSEWLLSQGYDVHGIVRRVALEDPDHRLSRLKEVRERLHLHAASLESYGSIHRVVHEVQPDE
CYHLAAQSFVSYSFDDEFSTLNANINGTHYLLSALKALTPDCRFYFAGSSEMFGMAETVPQNEDTRFHPRSSYGISKVAG
FDLTRNYREAYGMHASSGILFNHESPRRGFEFVTRKIAAGVARIVAGKSRELRLGNLDAKRDWGHAREYVTAMWMMLQQT
TPDDYVVATGDCHSVREFAELAFSHVGLDYTKYITTDPQLFRPAEVNLLKGDAAKAQRVLGWRHRVTFPELVREMVDEDC
RALGIG
>Mature_325_residues
ASKCALLTGITGQDGSYLSEWLLSQGYDVHGIVRRVALEDPDHRLSRLKEVRERLHLHAASLESYGSIHRVVHEVQPDEC
YHLAAQSFVSYSFDDEFSTLNANINGTHYLLSALKALTPDCRFYFAGSSEMFGMAETVPQNEDTRFHPRSSYGISKVAGF
DLTRNYREAYGMHASSGILFNHESPRRGFEFVTRKIAAGVARIVAGKSRELRLGNLDAKRDWGHAREYVTAMWMMLQQTT
PDDYVVATGDCHSVREFAELAFSHVGLDYTKYITTDPQLFRPAEVNLLKGDAAKAQRVLGWRHRVTFPELVREMVDEDCR
ALGIG

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=45.906432748538, Blast_Score=299, Evalue=2e-81,
Organism=Escherichia coli, GI1788366, Length=352, Percent_Identity=44.6022727272727, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI48994969, Length=332, Percent_Identity=26.2048192771084, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788353, Length=338, Percent_Identity=23.9644970414201, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=345, Percent_Identity=46.0869565217391, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI133901786, Length=345, Percent_Identity=46.0869565217391, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17539422, Length=345, Percent_Identity=46.0869565217391, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI133901788, Length=345, Percent_Identity=46.0869565217391, Blast_Score=300, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI133901790, Length=345, Percent_Identity=46.0869565217391, Blast_Score=300, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17507723, Length=343, Percent_Identity=46.064139941691, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24158427, Length=350, Percent_Identity=46.5714285714286, Blast_Score=306, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 36786; Mature: 36655

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKCALLTGITGQDGSYLSEWLLSQGYDVHGIVRRVALEDPDHRLSRLKEVRERLHLHA
CCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ASLESYGSIHRVVHEVQPDECYHLAAQSFVSYSFDDEFSTLNANINGTHYLLSALKALTP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHCCC
DCRFYFAGSSEMFGMAETVPQNEDTRFHPRSSYGISKVAGFDLTRNYREAYGMHASSGIL
CCEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCEE
FNHESPRRGFEFVTRKIAAGVARIVAGKSRELRLGNLDAKRDWGHAREYVTAMWMMLQQT
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCC
TPDDYVVATGDCHSVREFAELAFSHVGLDYTKYITTDPQLFRPAEVNLLKGDAAKAQRVL
CCCCEEEECCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCEEEECCHHHHHHHH
GWRHRVTFPELVREMVDEDCRALGIG
HHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASKCALLTGITGQDGSYLSEWLLSQGYDVHGIVRRVALEDPDHRLSRLKEVRERLHLHA
CCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ASLESYGSIHRVVHEVQPDECYHLAAQSFVSYSFDDEFSTLNANINGTHYLLSALKALTP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHCCC
DCRFYFAGSSEMFGMAETVPQNEDTRFHPRSSYGISKVAGFDLTRNYREAYGMHASSGIL
CCEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCEE
FNHESPRRGFEFVTRKIAAGVARIVAGKSRELRLGNLDAKRDWGHAREYVTAMWMMLQQT
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCC
TPDDYVVATGDCHSVREFAELAFSHVGLDYTKYITTDPQLFRPAEVNLLKGDAAKAQRVL
CCCCEEEECCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCEEEECCHHHHHHHH
GWRHRVTFPELVREMVDEDCRALGIG
HHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]