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The map label for this gene is pmbA [H]

Identifier: 116620144

GI number: 116620144

Start: 1262157

End: 1263509

Strand: Reverse

Name: pmbA [H]

Synonym: Acid_1017

Alternate gene names: 116620144

Gene position: 1263509-1262157 (Counterclockwise)

Preceding gene: 116620145

Following gene: 116620143

Centisome position: 12.68

GC content: 62.45

Gene sequence:

>1353_bases
TTGAACGGGAATCATCTATTGGAACTCGCGCAAAACATCATCCGGCAGGCGCAAGCCGCGGGCGCGAGCGACGCCGAATG
CACGATCGCGGAGGGCGAGGAGTTCTCGGCGAACGTGCGTATGCGGGAGGTCGAGAATCTGAAGGAAGCGGGCTCGCGCG
GCGCCGGACTCCGTATCCTGATCGGCAAGCACACCGGTGCTTCCTACACCAGCGATCTTTCGAGCGAGGGAATCGCCCAC
ATGGTGAAATCGGCCATCGAACTGGGCGATATCACGACGGAGGATCCCCATGCAGGGCTGCCCGATCCCGAAGATTTCGG
TTCGGTCCCAGGCGATCTCGGGATGTACTCGCCGGACATCGCACAGCTCGATACCGCGGTCAAGATCGACATGGCCAAGC
GGGCGGAGGAGGCGGCGCTGAGCAGCGATCCGCGCATCTCCAACTCCGAGGGCGCGAGTTTCGATAACTACGTGGGCCGG
CATGTCTTCGCCAATTCGCGCGGGTTCGCGGGCGAATACCGCTCAAGCTACTGTTCCCTGAGCACGTCACCGGTGGCGCG
CGATGGCGACTCGATGGAGCGCGACTACTGGTACACGATGGCGCGCACGTTCCCCGGATTAGAGGCGCCGGAGCATGTCG
GACGAATGGCGGCGCAGCGCGCGCTTCGGCGGCTGAACGCCGTGAAGGTGGAGACCCAGAAGGTGCCGGTGGTGTTCGAG
CCGCGCACGGCGCGATCGCTGCTGGACAACATCTTCGAAGCCGTCCACGGCATGTCGATCTACCGGCACGAGAGCTTCCT
GGCAAATAAACTTGGCCAGAAGGTGGCGTGCGACGGACTCACTGTAATCGACGACGGCACTATCCCGGGACTGTTCGGCA
CGTCGCCGTTCGACGATGAAGGCGTGGCCTCGCGGCGCACGGTAGTGATCGAGCGCGGCGTGCTGAAGAACTACCTGCTG
AATTCGTACGCGGCTCGCAAATTGGGGATGAAGACGACGGGCAACGCGTCGCGCGGGCTCACCGGGAATGCCGGCATAGG
GCACGGGAACTTCTTCATCGAGAAAGGCGTCCAAACGCCGGAGCAGATCATTGCGGGCATTCCGAACGGTTTTTACGTCA
CGGAATTGATGGGCTTCGGAGTCAACATCGTGACCGGCGATTATTCGCGGGGAGCGGCGGGACTGTGGATCCGCAATGGC
GAACTGGCGTTTGCGGTCAGCGAGGTGACGATCGCGGGCAACTTGAGTGAGATGCTGCTGGGCATCGAGGCGATCGGCAG
CGACCTGGACTTCCGCGGGTCGGTGGCAGCGCCTACAATCAAGATGGGAGAAATGACCGTTGGTGGCAAGTAA

Upstream 100 bases:

>100_bases
ATGAAGGCATCGGCACCTGCGGCAAGGACGGGCAGAGCGTGCCGGTGGGCGTCGGGATCCCGACCATCAAGATCGATCGT
ATGACCGTGGGAGGCACGGC

Downstream 100 bases:

>100_bases
AGCGCAACCCAAGAAAAGTGCGTCGATACCGCCGGCCGCGGTGGTGGTTGGCCTGGTGCTCGTGCTGGGTCTTGCCGGCT
TCCTTTATTTGGATCGCACG

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNGNHLLELAQNIIRQAQAAGASDAECTIAEGEEFSANVRMREVENLKEAGSRGAGLRILIGKHTGASYTSDLSSEGIAH
MVKSAIELGDITTEDPHAGLPDPEDFGSVPGDLGMYSPDIAQLDTAVKIDMAKRAEEAALSSDPRISNSEGASFDNYVGR
HVFANSRGFAGEYRSSYCSLSTSPVARDGDSMERDYWYTMARTFPGLEAPEHVGRMAAQRALRRLNAVKVETQKVPVVFE
PRTARSLLDNIFEAVHGMSIYRHESFLANKLGQKVACDGLTVIDDGTIPGLFGTSPFDDEGVASRRTVVIERGVLKNYLL
NSYAARKLGMKTTGNASRGLTGNAGIGHGNFFIEKGVQTPEQIIAGIPNGFYVTELMGFGVNIVTGDYSRGAAGLWIRNG
ELAFAVSEVTIAGNLSEMLLGIEAIGSDLDFRGSVAAPTIKMGEMTVGGK

Sequences:

>Translated_450_residues
MNGNHLLELAQNIIRQAQAAGASDAECTIAEGEEFSANVRMREVENLKEAGSRGAGLRILIGKHTGASYTSDLSSEGIAH
MVKSAIELGDITTEDPHAGLPDPEDFGSVPGDLGMYSPDIAQLDTAVKIDMAKRAEEAALSSDPRISNSEGASFDNYVGR
HVFANSRGFAGEYRSSYCSLSTSPVARDGDSMERDYWYTMARTFPGLEAPEHVGRMAAQRALRRLNAVKVETQKVPVVFE
PRTARSLLDNIFEAVHGMSIYRHESFLANKLGQKVACDGLTVIDDGTIPGLFGTSPFDDEGVASRRTVVIERGVLKNYLL
NSYAARKLGMKTTGNASRGLTGNAGIGHGNFFIEKGVQTPEQIIAGIPNGFYVTELMGFGVNIVTGDYSRGAAGLWIRNG
ELAFAVSEVTIAGNLSEMLLGIEAIGSDLDFRGSVAAPTIKMGEMTVGGK
>Mature_450_residues
MNGNHLLELAQNIIRQAQAAGASDAECTIAEGEEFSANVRMREVENLKEAGSRGAGLRILIGKHTGASYTSDLSSEGIAH
MVKSAIELGDITTEDPHAGLPDPEDFGSVPGDLGMYSPDIAQLDTAVKIDMAKRAEEAALSSDPRISNSEGASFDNYVGR
HVFANSRGFAGEYRSSYCSLSTSPVARDGDSMERDYWYTMARTFPGLEAPEHVGRMAAQRALRRLNAVKVETQKVPVVFE
PRTARSLLDNIFEAVHGMSIYRHESFLANKLGQKVACDGLTVIDDGTIPGLFGTSPFDDEGVASRRTVVIERGVLKNYLL
NSYAARKLGMKTTGNASRGLTGNAGIGHGNFFIEKGVQTPEQIIAGIPNGFYVTELMGFGVNIVTGDYSRGAAGLWIRNG
ELAFAVSEVTIAGNLSEMLLGIEAIGSDLDFRGSVAAPTIKMGEMTVGGK

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=433, Percent_Identity=38.568129330254, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1789640, Length=427, Percent_Identity=26.6978922716628, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48083; Mature: 48083

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGNHLLELAQNIIRQAQAAGASDAECTIAEGEEFSANVRMREVENLKEAGSRGAGLRIL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEE
IGKHTGASYTSDLSSEGIAHMVKSAIELGDITTEDPHAGLPDPEDFGSVPGDLGMYSPDI
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCH
AQLDTAVKIDMAKRAEEAALSSDPRISNSEGASFDNYVGRHVFANSRGFAGEYRSSYCSL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEECCCCCCHHHHHHHCCC
STSPVARDGDSMERDYWYTMARTFPGLEAPEHVGRMAAQRALRRLNAVKVETQKVPVVFE
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEC
PRTARSLLDNIFEAVHGMSIYRHESFLANKLGQKVACDGLTVIDDGTIPGLFGTSPFDDE
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCEEEEECCCCCCCCCCCCCCCC
GVASRRTVVIERGVLKNYLLNSYAARKLGMKTTGNASRGLTGNAGIGHGNFFIEKGVQTP
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCH
EQIIAGIPNGFYVTELMGFGVNIVTGDYSRGAAGLWIRNGELAFAVSEVTIAGNLSEMLL
HHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCEEEEECCCEEEEEEEEEEECCHHHHHH
GIEAIGSDLDFRGSVAAPTIKMGEMTVGGK
HHHHHCCCCCCCCCCCCCEEEECCEECCCC
>Mature Secondary Structure
MNGNHLLELAQNIIRQAQAAGASDAECTIAEGEEFSANVRMREVENLKEAGSRGAGLRIL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEE
IGKHTGASYTSDLSSEGIAHMVKSAIELGDITTEDPHAGLPDPEDFGSVPGDLGMYSPDI
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCH
AQLDTAVKIDMAKRAEEAALSSDPRISNSEGASFDNYVGRHVFANSRGFAGEYRSSYCSL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEECCCCCCHHHHHHHCCC
STSPVARDGDSMERDYWYTMARTFPGLEAPEHVGRMAAQRALRRLNAVKVETQKVPVVFE
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEC
PRTARSLLDNIFEAVHGMSIYRHESFLANKLGQKVACDGLTVIDDGTIPGLFGTSPFDDE
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCEEEEECCCCCCCCCCCCCCCC
GVASRRTVVIERGVLKNYLLNSYAARKLGMKTTGNASRGLTGNAGIGHGNFFIEKGVQTP
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCH
EQIIAGIPNGFYVTELMGFGVNIVTGDYSRGAAGLWIRNGELAFAVSEVTIAGNLSEMLL
HHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCEEEEECCCEEEEEEEEEEECCHHHHHH
GIEAIGSDLDFRGSVAAPTIKMGEMTVGGK
HHHHHCCCCCCCCCCCCCEEEECCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]