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The map label for this gene is trpE(G) [H]

Identifier: 116620114

GI number: 116620114

Start: 1229396

End: 1231567

Strand: Reverse

Name: trpE(G) [H]

Synonym: Acid_0987

Alternate gene names: 116620114

Gene position: 1231567-1229396 (Counterclockwise)

Preceding gene: 116620115

Following gene: 116620113

Centisome position: 12.36

GC content: 64.36

Gene sequence:

>2172_bases
ATGCGCGAATACCGCTATACGACTCCCCACGGGATCCAGGTCACCCGCACCGTTTCGAAGACCAATTTCCGGAAGGGGCT
GACGCACCTTCTTAGCGATCTGGACCAGCACCGTGGGATTTATCTATCCTCGGGATACGAGTATCCCGGCAGATACTCGC
GCTGGGATATCGCATCCACATGCCCGCCGCTCGAGATCATCGCCTACGACCGGCGCTTTGAACTCCGCCCGCTGAATCAG
CGCGGCCGGGAAATCCTGCGGCTGTTCCATCCGCTCCTCGCCAAACTTCCCCACTGGGACGCATTCGACTATGAAGGCGA
GATGCTCGCCGGACGCCTGAAGCCGCTGGCGGCGCTGTTCTCCGAAGAGGAGCGCAGCAAGCAGCCCTCCGCATTTTCGA
TTCTGCGCGCTCTTATCGAGGAATTCCGCGGCGAGGAGAACTCCCGTCTCGGGCTCGTGGGCGCCTTCGGCTACGATCTG
CTGTTCCAGTTCGAACCCATCGAAAGGAAGCTGCCGCGCCACGGCCACAAAGATCTGCACCTGTTCCTCTGCGACGACAT
CTGGTACATGGATCGCAAGAAAGAGCAGGTCGAGCGCTTCCAGTACGAATTCCAGGGGGAAGGCGCCAGCACCGTGGGAC
TGCCGCGCGAGGGCGAACTGGTGCCGCCGCCCGCGCCCGCTCCGGCCGGACCCATCACCAGCGATCACACGCCCGAAGAG
TACATGGCCAACGTGGAAAAGGTGCGCGAGGGCATGCGCCGCGGCGATTATTACGAAGTCGTGCTGCGCCAGACCTTCCG
CACGCCCTTCTCCGGCAAGGCCAGCGAACTCTTCCGCCGCGTCCAAACTGCCAGCCCGAGCCCTTACGAATTCCTCCTGC
AGTTTGGCGAGGAACAACTGGTAGGCGCGTCGCCCGAGATGTTCGTCAGGGTGGAAGGCGCGCGCGTCGAGACCTGCCCC
ATCTCGGGCACCGCGCAGCGCACCGGCGACCCCTTGCGCGACGCTGACAACATCCGCGAACTCCTGGTCAGCACCAAGGA
AGAGAGCGAGCTGACCATGTGCACGGACGTGGACCGCAACGATAAGTCGCGCGTCTGCGAACCGGGCACGGTCAAAGTCA
TCGGGCGCCGCTTGATCGAAAGCTACGCCGGCGTGTTTCACACCGTGGATCACGTGGAGGGAATCCTCCAGGAGGGCTTC
GACGCCCTGGATGCCTTTCTCAGCCACATGTGGGCCGTGACCGTAATCGGCGCTCCCAAGAAGGCGGCCGCACAAACCGT
CGAAGCGCTTGAACGGAACGCGCGGGGCTGGTATGGCGGCGCGGTGGGGATGATCTCGCTCGGCGGCGACATCAACACCG
GCATCCTGATCCGCACGACCTATCTGCGTGACGGCGTGGCCAGCTATCCCGTCGGCGCCACTCTCCTGTTCGATTCCGTG
CCGGTCATGGAAGAGCGCGAAACACGCCTCAAAGCCACCGGTTTTTTCCGGACCCTCGGCACGCCCGAAGCCAAGGCCGC
GGCCGGGGCGCTGGAGCACGCCGCCGGCGGCGCGGGTGCGCGCCTGCTGCTGGTGGATAACGACGATTGCTTCATCCACA
CGCTTGCCAACTACGCGCGCCAGACCGGCGCCGAAGTGGTCACGTACCGCGCGGGCTTTCCGCCGGAGTTTATCGCGCAG
GTGGCGCCGAACCTGATTCTGATCTCGCCCGGTCCGGGACGTCCGGCCGATTTCGGCGTCCCCGATGTCGTGAAGACGGC
CGTCCGCCTGGGCGTGCCGGTGTTCGGGGTGTGCCTGGGCTTGCAAGGCATAGTCGAGGCCTTTGGTGGTGAACTCGGTG
TGCTCGATTATCCAATGCATGGCAAACCTTCCACCGTGCGCAATCGCGGCGTCGGCGTGTTCCAAGGGCTGCCTGCGGAG
TTTCAAGTGGGCCGCTACCACTCGCTCTTCGCGCGCCGCGAGACCTTCCCCAGCTGCCTGGAAATCACCGCGGAAACAGA
GGACGGCGTGATCATGGGCGTCCGCCATCGCGAACTTCCGGTAGAGGCCGTGCAGTTCCACCCGGAATCGATTCTGACCG
CCGGGGGCGATCACGGTCTCAGACTCATGGCCAATGCCATGCGCCTCGCGAAGGCCGCCGCGGCCGTGCAGGTTCGAAAC
GTTGACCGCTGA

Upstream 100 bases:

>100_bases
GAAAAATCCGGTCACGAACTGAAGGCGGCCGAGCAGATCGGCGAAGAGATTCTGGCCCGCATGCTATCCTGAGAAATATT
TTTCCCAAAAGGGCGTGGCT

Downstream 100 bases:

>100_bases
CTCCGCTGACCGGTCAACCCTGCGCGGCTGCGCGCTCCTGGCGGCGCTCCTGGTATTGATCGGCGCGCTGCGCATCGTTT
CCAGCTACACCGCGTTGAGC

Product: anthranilate synthase

Products: NA

Alternate protein names: Glutamine amidotransferase [H]

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MREYRYTTPHGIQVTRTVSKTNFRKGLTHLLSDLDQHRGIYLSSGYEYPGRYSRWDIASTCPPLEIIAYDRRFELRPLNQ
RGREILRLFHPLLAKLPHWDAFDYEGEMLAGRLKPLAALFSEEERSKQPSAFSILRALIEEFRGEENSRLGLVGAFGYDL
LFQFEPIERKLPRHGHKDLHLFLCDDIWYMDRKKEQVERFQYEFQGEGASTVGLPREGELVPPPAPAPAGPITSDHTPEE
YMANVEKVREGMRRGDYYEVVLRQTFRTPFSGKASELFRRVQTASPSPYEFLLQFGEEQLVGASPEMFVRVEGARVETCP
ISGTAQRTGDPLRDADNIRELLVSTKEESELTMCTDVDRNDKSRVCEPGTVKVIGRRLIESYAGVFHTVDHVEGILQEGF
DALDAFLSHMWAVTVIGAPKKAAAQTVEALERNARGWYGGAVGMISLGGDINTGILIRTTYLRDGVASYPVGATLLFDSV
PVMEERETRLKATGFFRTLGTPEAKAAAGALEHAAGGAGARLLLVDNDDCFIHTLANYARQTGAEVVTYRAGFPPEFIAQ
VAPNLILISPGPGRPADFGVPDVVKTAVRLGVPVFGVCLGLQGIVEAFGGELGVLDYPMHGKPSTVRNRGVGVFQGLPAE
FQVGRYHSLFARRETFPSCLEITAETEDGVIMGVRHRELPVEAVQFHPESILTAGGDHGLRLMANAMRLAKAAAAVQVRN
VDR

Sequences:

>Translated_723_residues
MREYRYTTPHGIQVTRTVSKTNFRKGLTHLLSDLDQHRGIYLSSGYEYPGRYSRWDIASTCPPLEIIAYDRRFELRPLNQ
RGREILRLFHPLLAKLPHWDAFDYEGEMLAGRLKPLAALFSEEERSKQPSAFSILRALIEEFRGEENSRLGLVGAFGYDL
LFQFEPIERKLPRHGHKDLHLFLCDDIWYMDRKKEQVERFQYEFQGEGASTVGLPREGELVPPPAPAPAGPITSDHTPEE
YMANVEKVREGMRRGDYYEVVLRQTFRTPFSGKASELFRRVQTASPSPYEFLLQFGEEQLVGASPEMFVRVEGARVETCP
ISGTAQRTGDPLRDADNIRELLVSTKEESELTMCTDVDRNDKSRVCEPGTVKVIGRRLIESYAGVFHTVDHVEGILQEGF
DALDAFLSHMWAVTVIGAPKKAAAQTVEALERNARGWYGGAVGMISLGGDINTGILIRTTYLRDGVASYPVGATLLFDSV
PVMEERETRLKATGFFRTLGTPEAKAAAGALEHAAGGAGARLLLVDNDDCFIHTLANYARQTGAEVVTYRAGFPPEFIAQ
VAPNLILISPGPGRPADFGVPDVVKTAVRLGVPVFGVCLGLQGIVEAFGGELGVLDYPMHGKPSTVRNRGVGVFQGLPAE
FQVGRYHSLFARRETFPSCLEITAETEDGVIMGVRHRELPVEAVQFHPESILTAGGDHGLRLMANAMRLAKAAAAVQVRN
VDR
>Mature_723_residues
MREYRYTTPHGIQVTRTVSKTNFRKGLTHLLSDLDQHRGIYLSSGYEYPGRYSRWDIASTCPPLEIIAYDRRFELRPLNQ
RGREILRLFHPLLAKLPHWDAFDYEGEMLAGRLKPLAALFSEEERSKQPSAFSILRALIEEFRGEENSRLGLVGAFGYDL
LFQFEPIERKLPRHGHKDLHLFLCDDIWYMDRKKEQVERFQYEFQGEGASTVGLPREGELVPPPAPAPAGPITSDHTPEE
YMANVEKVREGMRRGDYYEVVLRQTFRTPFSGKASELFRRVQTASPSPYEFLLQFGEEQLVGASPEMFVRVEGARVETCP
ISGTAQRTGDPLRDADNIRELLVSTKEESELTMCTDVDRNDKSRVCEPGTVKVIGRRLIESYAGVFHTVDHVEGILQEGF
DALDAFLSHMWAVTVIGAPKKAAAQTVEALERNARGWYGGAVGMISLGGDINTGILIRTTYLRDGVASYPVGATLLFDSV
PVMEERETRLKATGFFRTLGTPEAKAAAGALEHAAGGAGARLLLVDNDDCFIHTLANYARQTGAEVVTYRAGFPPEFIAQ
VAPNLILISPGPGRPADFGVPDVVKTAVRLGVPVFGVCLGLQGIVEAFGGELGVLDYPMHGKPSTVRNRGVGVFQGLPAE
FQVGRYHSLFARRETFPSCLEITAETEDGVIMGVRHRELPVEAVQFHPESILTAGGDHGLRLMANAMRLAKAAAAVQVRN
VDR

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=410, Percent_Identity=31.7073170731707, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789760, Length=190, Percent_Identity=42.1052631578947, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788114, Length=390, Percent_Identity=27.1794871794872, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1787517, Length=196, Percent_Identity=39.2857142857143, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1786809, Length=230, Percent_Identity=28.695652173913, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322638, Length=198, Percent_Identity=42.9292929292929, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320935, Length=370, Percent_Identity=29.7297297297297, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324361, Length=125, Percent_Identity=34.4, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR006805
- InterPro:   IPR006220
- InterPro:   IPR010112
- InterPro:   IPR001317
- InterPro:   IPR015890
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 80165; Mature: 80165

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00626 RCC1_2 ; PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREYRYTTPHGIQVTRTVSKTNFRKGLTHLLSDLDQHRGIYLSSGYEYPGRYSRWDIAST
CCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCC
CPPLEIIAYDRRFELRPLNQRGREILRLFHPLLAKLPHWDAFDYEGEMLAGRLKPLAALF
CCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
SEEERSKQPSAFSILRALIEEFRGEENSRLGLVGAFGYDLLFQFEPIERKLPRHGHKDLH
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCHHHHCCCCCCCCEE
LFLCDDIWYMDRKKEQVERFQYEFQGEGASTVGLPREGELVPPPAPAPAGPITSDHTPEE
EEEECCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
YMANVEKVREGMRRGDYYEVVLRQTFRTPFSGKASELFRRVQTASPSPYEFLLQFGEEQL
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCHHH
VGASPEMFVRVEGARVETCPISGTAQRTGDPLRDADNIRELLVSTKEESELTMCTDVDRN
CCCCCCEEEEEECCEEEECCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCC
DKSRVCEPGTVKVIGRRLIESYAGVFHTVDHVEGILQEGFDALDAFLSHMWAVTVIGAPK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCH
KAAAQTVEALERNARGWYGGAVGMISLGGDINTGILIRTTYLRDGVASYPVGATLLFDSV
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEHHHCCCCCCCCCCEEEECCC
PVMEERETRLKATGFFRTLGTPEAKAAAGALEHAAGGAGARLLLVDNDDCFIHTLANYAR
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHH
QTGAEVVTYRAGFPPEFIAQVAPNLILISPGPGRPADFGVPDVVKTAVRLGVPVFGVCLG
HCCCCEEEEECCCCHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LQGIVEAFGGELGVLDYPMHGKPSTVRNRGVGVFQGLPAEFQVGRYHSLFARRETFPSCL
HHHHHHHCCCCCCEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
EITAETEDGVIMGVRHRELPVEAVQFHPESILTAGGDHGLRLMANAMRLAKAAAAVQVRN
EEEECCCCCEEEEECCCCCCHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
VDR
CCC
>Mature Secondary Structure
MREYRYTTPHGIQVTRTVSKTNFRKGLTHLLSDLDQHRGIYLSSGYEYPGRYSRWDIAST
CCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCC
CPPLEIIAYDRRFELRPLNQRGREILRLFHPLLAKLPHWDAFDYEGEMLAGRLKPLAALF
CCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
SEEERSKQPSAFSILRALIEEFRGEENSRLGLVGAFGYDLLFQFEPIERKLPRHGHKDLH
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCHHHHCCCCCCCCEE
LFLCDDIWYMDRKKEQVERFQYEFQGEGASTVGLPREGELVPPPAPAPAGPITSDHTPEE
EEEECCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
YMANVEKVREGMRRGDYYEVVLRQTFRTPFSGKASELFRRVQTASPSPYEFLLQFGEEQL
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCHHH
VGASPEMFVRVEGARVETCPISGTAQRTGDPLRDADNIRELLVSTKEESELTMCTDVDRN
CCCCCCEEEEEECCEEEECCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCC
DKSRVCEPGTVKVIGRRLIESYAGVFHTVDHVEGILQEGFDALDAFLSHMWAVTVIGAPK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCH
KAAAQTVEALERNARGWYGGAVGMISLGGDINTGILIRTTYLRDGVASYPVGATLLFDSV
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEHHHCCCCCCCCCCEEEECCC
PVMEERETRLKATGFFRTLGTPEAKAAAGALEHAAGGAGARLLLVDNDDCFIHTLANYAR
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHH
QTGAEVVTYRAGFPPEFIAQVAPNLILISPGPGRPADFGVPDVVKTAVRLGVPVFGVCLG
HCCCCEEEEECCCCHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LQGIVEAFGGELGVLDYPMHGKPSTVRNRGVGVFQGLPAEFQVGRYHSLFARRETFPSCL
HHHHHHHCCCCCCEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
EITAETEDGVIMGVRHRELPVEAVQFHPESILTAGGDHGLRLMANAMRLAKAAAAVQVRN
EEEECCCCCEEEEECCCCCCHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
VDR
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656657; 11481430 [H]