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The map label for this gene is dnaK [H]

Identifier: 116620104

GI number: 116620104

Start: 1214964

End: 1216769

Strand: Direct

Name: dnaK [H]

Synonym: Acid_0977

Alternate gene names: 116620104

Gene position: 1214964-1216769 (Clockwise)

Preceding gene: 116620103

Following gene: 116620106

Centisome position: 12.19

GC content: 66.33

Gene sequence:

>1806_bases
GTGATCGGGATCGATCTCGGCACCACCAATTCCGGTGTGGCCTATGCGGAGATCCTCGCCGATCCAGACCCGTTTGCGCC
CGCCAACGTGCAGTTGCTCGCCATTCCGCAGCTCACCAATCCCGGCGAAGTGCGCGAGGAAGATCTGCTGCCCTCGTTCC
TCTATCTGCCCGGCTCCAGTGATTTTCCCGCGGGCAGCATCGCGCTGCCGTGGGACGAAGGGGCCACCGTGGTCACGGGC
CGGCTGGCGCAAAAACGAGGCGTCGAAAATACCGGCCGGCTGGTCTCTTCGGCCAAGAGCTGGCTTTCACACTCGGGTGT
GGACCGGACATCGCCGCTGCTTCCCTTCCGCGCGCCGGAAGGCGTGGAAAAGATCTCGCCGGTGGCTGCGAGCCGCCGCT
ACCTGGAGCACATGCGCCAGGCGTGGGACGCCAAGATGCCGGACGCGCCCTTCCAGCAGCAGCAGATCCTGGTGACGGTG
CCGGCGTCTTTCGACGCGGTGGCGCGCGAACTCACGCTGGAGGCCGCTCAGCAGGCGGGCTATCAAAACATCACGCTGCT
CGAAGAACCGCAGGCCGCCTTCTACGCGTGGATCGAGCGGCACCCCGACTGGCGCCAGCGCGTCACGGTGGGCGACCTGA
TCCTGGTGGTGGATATCGGCGGCGGCACCACGGATTTCACGCTGATCGCGGTTACAGAAGCGAATGGCGAACTGAGCCTC
AACCGCGTGGCGGTGGGGGATCACATTCTGTTGGGCGGCGATAACATCGATCTCGCGCTCGCCGGAGTAGTGGCGCAGCG
GCTGGCCGAGAAGGGCACAAAAATCGACAGCCGGCAACACCAGGCGCTGTGGGCGAACTGCCGCGTCGCGAAGGAAAAGC
TGCTCGAGCCCGGCGCGAAGACCAGGGAACAGCCGGTCACGATTCTGGGCAAAGGCAGCGGCCTGGTCGGCGGCACTATT
AAGGCGACGCTGCTTACGGACGATATTGACCGCGTCCTGGGCGAGGGCTTTCTGCCCACTGTCGCGAGCACCGACATGCC
CGCCGTGCAGAGGCGTGTCGGACTCCAGGAACTCGGACTGCCCTATGCGGCCGACCCGGCGATCACGCGGCACATGGCGC
GCTTCCTGCGCCAGCAGGCCGCCAGCGTGGAACACGGCGCGGTCCGGCGCGGCCCCAGCGGGATCGCGGCGCCGACGCAC
GTGCTGTTCAACGGCGGCGTGCTCAATGCTGACCTGGTGCGTGAGCGCGTGCTCGCCACGCTCGATTCCTGGCTGGCCGC
GGAGGGACTGCCGCCGGTCAAGCCGTTGAGCGGCGAAGATCTGATGCACGCGGTTTCGCGCGGCGCGGCGTATTACGGGC
TGGCGCGTACCGGCCGCGGCGTGCGCATTCGCGGCGGCGTCCCACGCACTTACTATGTGGGCGTGGAGAGCGCGATGCCG
AGCATCCCCGGCTTTCCGGCGCCGCTGAAGGCACTCGCGGTAGTGCCATTCGGGATGGAGGAAGGCACCGAGACGCGGAT
TCCCGGCCGCGAATTCGGCTTGGTGATCGGACAGCCGGCGGAGTTCCGCTTCTTCACCAGCGCCTCGCGCAAGAACGACC
AGCCCGGAGACCTGATTGAAGATTACGGCGACGCGCTCGAAGAACTCTCGCCGATGGAGGTAGCGTTCGAATCGGGCGAG
ACTTCCGAGGTTGTGCCGGTCGCGTTTGAGAGCGTGGTCACGGAAACCGGTATGTTGCAGCTATGGTGCGTGGCGCGTGA
CGGACGCCGCTGGAAGCTGGAATTCAACGTTCGCGAAAGGGTGTAG

Upstream 100 bases:

>100_bases
ACGAAGGTTGATATGCCTGCGCTGCCGGCGCGCCTGGACGCCACCATCATCGCCCCCGCGGAAATTGAAATTGAGTAAGA
GAGCCGCAGTACCCAAGTAC

Downstream 100 bases:

>100_bases
GGGCTAGCGGCCGCGCTGTTTTTCCTGAAGCGTGCGTCGCAGGACAGGTAAGACGGCGCGGTCCTTTTCGCCGGCGAGCT
CTTCCTTCAACGCCACGATC

Product: putative heat-shock chaperone protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MIGIDLGTTNSGVAYAEILADPDPFAPANVQLLAIPQLTNPGEVREEDLLPSFLYLPGSSDFPAGSIALPWDEGATVVTG
RLAQKRGVENTGRLVSSAKSWLSHSGVDRTSPLLPFRAPEGVEKISPVAASRRYLEHMRQAWDAKMPDAPFQQQQILVTV
PASFDAVARELTLEAAQQAGYQNITLLEEPQAAFYAWIERHPDWRQRVTVGDLILVVDIGGGTTDFTLIAVTEANGELSL
NRVAVGDHILLGGDNIDLALAGVVAQRLAEKGTKIDSRQHQALWANCRVAKEKLLEPGAKTREQPVTILGKGSGLVGGTI
KATLLTDDIDRVLGEGFLPTVASTDMPAVQRRVGLQELGLPYAADPAITRHMARFLRQQAASVEHGAVRRGPSGIAAPTH
VLFNGGVLNADLVRERVLATLDSWLAAEGLPPVKPLSGEDLMHAVSRGAAYYGLARTGRGVRIRGGVPRTYYVGVESAMP
SIPGFPAPLKALAVVPFGMEEGTETRIPGREFGLVIGQPAEFRFFTSASRKNDQPGDLIEDYGDALEELSPMEVAFESGE
TSEVVPVAFESVVTETGMLQLWCVARDGRRWKLEFNVRERV

Sequences:

>Translated_601_residues
MIGIDLGTTNSGVAYAEILADPDPFAPANVQLLAIPQLTNPGEVREEDLLPSFLYLPGSSDFPAGSIALPWDEGATVVTG
RLAQKRGVENTGRLVSSAKSWLSHSGVDRTSPLLPFRAPEGVEKISPVAASRRYLEHMRQAWDAKMPDAPFQQQQILVTV
PASFDAVARELTLEAAQQAGYQNITLLEEPQAAFYAWIERHPDWRQRVTVGDLILVVDIGGGTTDFTLIAVTEANGELSL
NRVAVGDHILLGGDNIDLALAGVVAQRLAEKGTKIDSRQHQALWANCRVAKEKLLEPGAKTREQPVTILGKGSGLVGGTI
KATLLTDDIDRVLGEGFLPTVASTDMPAVQRRVGLQELGLPYAADPAITRHMARFLRQQAASVEHGAVRRGPSGIAAPTH
VLFNGGVLNADLVRERVLATLDSWLAAEGLPPVKPLSGEDLMHAVSRGAAYYGLARTGRGVRIRGGVPRTYYVGVESAMP
SIPGFPAPLKALAVVPFGMEEGTETRIPGREFGLVIGQPAEFRFFTSASRKNDQPGDLIEDYGDALEELSPMEVAFESGE
TSEVVPVAFESVVTETGMLQLWCVARDGRRWKLEFNVRERV
>Mature_601_residues
MIGIDLGTTNSGVAYAEILADPDPFAPANVQLLAIPQLTNPGEVREEDLLPSFLYLPGSSDFPAGSIALPWDEGATVVTG
RLAQKRGVENTGRLVSSAKSWLSHSGVDRTSPLLPFRAPEGVEKISPVAASRRYLEHMRQAWDAKMPDAPFQQQQILVTV
PASFDAVARELTLEAAQQAGYQNITLLEEPQAAFYAWIERHPDWRQRVTVGDLILVVDIGGGTTDFTLIAVTEANGELSL
NRVAVGDHILLGGDNIDLALAGVVAQRLAEKGTKIDSRQHQALWANCRVAKEKLLEPGAKTREQPVTILGKGSGLVGGTI
KATLLTDDIDRVLGEGFLPTVASTDMPAVQRRVGLQELGLPYAADPAITRHMARFLRQQAASVEHGAVRRGPSGIAAPTH
VLFNGGVLNADLVRERVLATLDSWLAAEGLPPVKPLSGEDLMHAVSRGAAYYGLARTGRGVRIRGGVPRTYYVGVESAMP
SIPGFPAPLKALAVVPFGMEEGTETRIPGREFGLVIGQPAEFRFFTSASRKNDQPGDLIEDYGDALEELSPMEVAFESGE
TSEVVPVAFESVVTETGMLQLWCVARDGRRWKLEFNVRERV

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Escherichia coli, GI1786196, Length=307, Percent_Identity=25.7328990228013, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 64807; Mature: 64807

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00297 HSP70_1 ; PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGIDLGTTNSGVAYAEILADPDPFAPANVQLLAIPQLTNPGEVREEDLLPSFLYLPGSS
CEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCEEECCCCC
DFPAGSIALPWDEGATVVTGRLAQKRGVENTGRLVSSAKSWLSHSGVDRTSPLLPFRAPE
CCCCCCEEECCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GVEKISPVAASRRYLEHMRQAWDAKMPDAPFQQQQILVTVPASFDAVARELTLEAAQQAG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
YQNITLLEEPQAAFYAWIERHPDWRQRVTVGDLILVVDIGGGTTDFTLIAVTEANGELSL
CCCEEEEECCHHHHHHHHHCCCCHHHEEEECCEEEEEECCCCCCCEEEEEEEECCCCEEE
NRVAVGDHILLGGDNIDLALAGVVAQRLAEKGTKIDSRQHQALWANCRVAKEKLLEPGAK
EEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TREQPVTILGKGSGLVGGTIKATLLTDDIDRVLGEGFLPTVASTDMPAVQRRVGLQELGL
CCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHCC
PYAADPAITRHMARFLRQQAASVEHGAVRRGPSGIAAPTHVLFNGGVLNADLVRERVLAT
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
LDSWLAAEGLPPVKPLSGEDLMHAVSRGAAYYGLARTGRGVRIRGGVPRTYYVGVESAMP
HHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEHHHCCCCEEEECCCCEEEEEEHHHCCC
SIPGFPAPLKALAVVPFGMEEGTETRIPGREFGLVIGQPAEFRFFTSASRKNDQPGDLIE
CCCCCCCCCHHHEEECCCCCCCCCCCCCCHHCCEEECCCCCEEEEECCCCCCCCCCHHHH
DYGDALEELSPMEVAFESGETSEVVPVAFESVVTETGMLQLWCVARDGRRWKLEFNVRER
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECHHCC
V
C
>Mature Secondary Structure
MIGIDLGTTNSGVAYAEILADPDPFAPANVQLLAIPQLTNPGEVREEDLLPSFLYLPGSS
CEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCEEECCCCC
DFPAGSIALPWDEGATVVTGRLAQKRGVENTGRLVSSAKSWLSHSGVDRTSPLLPFRAPE
CCCCCCEEECCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GVEKISPVAASRRYLEHMRQAWDAKMPDAPFQQQQILVTVPASFDAVARELTLEAAQQAG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
YQNITLLEEPQAAFYAWIERHPDWRQRVTVGDLILVVDIGGGTTDFTLIAVTEANGELSL
CCCEEEEECCHHHHHHHHHCCCCHHHEEEECCEEEEEECCCCCCCEEEEEEEECCCCEEE
NRVAVGDHILLGGDNIDLALAGVVAQRLAEKGTKIDSRQHQALWANCRVAKEKLLEPGAK
EEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TREQPVTILGKGSGLVGGTIKATLLTDDIDRVLGEGFLPTVASTDMPAVQRRVGLQELGL
CCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHCC
PYAADPAITRHMARFLRQQAASVEHGAVRRGPSGIAAPTHVLFNGGVLNADLVRERVLAT
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
LDSWLAAEGLPPVKPLSGEDLMHAVSRGAAYYGLARTGRGVRIRGGVPRTYYVGVESAMP
HHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEHHHCCCCEEEECCCCEEEEEEHHHCCC
SIPGFPAPLKALAVVPFGMEEGTETRIPGREFGLVIGQPAEFRFFTSASRKNDQPGDLIE
CCCCCCCCCHHHEEECCCCCCCCCCCCCCHHCCEEECCCCCEEEEECCCCCCCCCCHHHH
DYGDALEELSPMEVAFESGETSEVVPVAFESVVTETGMLQLWCVARDGRRWKLEFNVRER
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECHHCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]