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The map label for this gene is trmJ [H]

Identifier: 116620037

GI number: 116620037

Start: 1136956

End: 1137714

Strand: Direct

Name: trmJ [H]

Synonym: Acid_0909

Alternate gene names: 116620037

Gene position: 1136956-1137714 (Clockwise)

Preceding gene: 116620029

Following gene: 116620040

Centisome position: 11.41

GC content: 63.37

Gene sequence:

>759_bases
ATGCCGCCTTTTGGTCCTGCGCTACCATTAAACATCAACGCCAACTGGAAAAACAACCTGCGGGTGGTACTGGTTTCCAC
GCGCAATCCGCTCAATATCGGTGCCGCCGCACGCGCGATGGCGAATTTCGGGTTCTCCCGCCTAGGCGTCGTGGCGCCGT
ATGATGTGGCTTTCCGTGAGGCACGCTCGGCCGTCGGCGCCGCGCCCCTGCTCCAGAAGGCGGAAGAATTCCCGAGCGTC
GCCGCCGCCGTTGCCGACTGCCGTCTCGTGGTCGGCACTACCAGCGTCGGTCACCGCGAACTGCAGCACCCCATTCGCCG
GCTCGAGTACGGGGCGCGCCTGATGCTGAAGGGGCTGCCTGCGGCCCCCGTAGCGCTGCTCTTCGGGAGCGAGAAGTTCG
GCCTCTCCAATGAGGACCTGAGTCACTGCCACTGGCTGATGCGCATCCCGACGGTCGGCCAGGATCTCTCGATGAACCTC
GGGCAGGCGGTCGCTCTCTGCCTTTACGAATTGGCGCGTGATCCTAAGGCCGCGCTCGCCAGCCCGGAAAAAATCCGGTC
CGCCAATGCCGGCGAGCTCGAACAGATCACCACCCGCCTGCTCGAGGTGCTCAACCGGAGCGGCTACGTCAATCCCGTGA
CATCGATTTCGACCGACGAAAAAGTCCGCCGCCTGGTGCGCCGCCTGGAAATTCCCGGACGCGATGCCAAGGTAATCCTG
GGAATGCTGCGCCAGATTTTGTGGAAGCTGGAAAACTGA

Upstream 100 bases:

>100_bases
CTATTCGCGTTGCAAAATACTTAGTCTAATGGGTTTGGGGATTCGTTGTCAAATCGGGTTACAAATTGAAGGCGCACACC
TGCCCCGCACCCAATGCGTG

Downstream 100 bases:

>100_bases
ATCGTTCTCAGGTCAGGTGGGCCAGCATGTCCGCGGTCCGGGTGACCGTGCAATACTCGCCGTCCAGGTTGGCCAGGCTC
ATCGCGTGAACCTCGCTCGC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MPPFGPALPLNINANWKNNLRVVLVSTRNPLNIGAAARAMANFGFSRLGVVAPYDVAFREARSAVGAAPLLQKAEEFPSV
AAAVADCRLVVGTTSVGHRELQHPIRRLEYGARLMLKGLPAAPVALLFGSEKFGLSNEDLSHCHWLMRIPTVGQDLSMNL
GQAVALCLYELARDPKAALASPEKIRSANAGELEQITTRLLEVLNRSGYVNPVTSISTDEKVRRLVRRLEIPGRDAKVIL
GMLRQILWKLEN

Sequences:

>Translated_252_residues
MPPFGPALPLNINANWKNNLRVVLVSTRNPLNIGAAARAMANFGFSRLGVVAPYDVAFREARSAVGAAPLLQKAEEFPSV
AAAVADCRLVVGTTSVGHRELQHPIRRLEYGARLMLKGLPAAPVALLFGSEKFGLSNEDLSHCHWLMRIPTVGQDLSMNL
GQAVALCLYELARDPKAALASPEKIRSANAGELEQITTRLLEVLNRSGYVNPVTSISTDEKVRRLVRRLEIPGRDAKVIL
GMLRQILWKLEN
>Mature_251_residues
PPFGPALPLNINANWKNNLRVVLVSTRNPLNIGAAARAMANFGFSRLGVVAPYDVAFREARSAVGAAPLLQKAEEFPSVA
AAVADCRLVVGTTSVGHRELQHPIRRLEYGARLMLKGLPAAPVALLFGSEKFGLSNEDLSHCHWLMRIPTVGQDLSMNLG
QAVALCLYELARDPKAALASPEKIRSANAGELEQITTRLLEVLNRSGYVNPVTSISTDEKVRRLVRRLEIPGRDAKVILG
MLRQILWKLEN

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=234, Percent_Identity=29.9145299145299, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1790865, Length=156, Percent_Identity=34.6153846153846, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27532; Mature: 27401

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPFGPALPLNINANWKNNLRVVLVSTRNPLNIGAAARAMANFGFSRLGVVAPYDVAFRE
CCCCCCCEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCHHHHHHH
ARSAVGAAPLLQKAEEFPSVAAAVADCRLVVGTTSVGHRELQHPIRRLEYGARLMLKGLP
HHHHHCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC
AAPVALLFGSEKFGLSNEDLSHCHWLMRIPTVGQDLSMNLGQAVALCLYELARDPKAALA
CCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHCCCHHHHC
SPEKIRSANAGELEQITTRLLEVLNRSGYVNPVTSISTDEKVRRLVRRLEIPGRDAKVIL
CHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
GMLRQILWKLEN
HHHHHHHHHCCC
>Mature Secondary Structure 
PPFGPALPLNINANWKNNLRVVLVSTRNPLNIGAAARAMANFGFSRLGVVAPYDVAFRE
CCCCCCEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCHHHHHHH
ARSAVGAAPLLQKAEEFPSVAAAVADCRLVVGTTSVGHRELQHPIRRLEYGARLMLKGLP
HHHHHCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC
AAPVALLFGSEKFGLSNEDLSHCHWLMRIPTVGQDLSMNLGQAVALCLYELARDPKAALA
CCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHCCCHHHHC
SPEKIRSANAGELEQITTRLLEVLNRSGYVNPVTSISTDEKVRRLVRRLEIPGRDAKVIL
CHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
GMLRQILWKLEN
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA