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The map label for this gene is cspLA [H]

Identifier: 116619951

GI number: 116619951

Start: 1022793

End: 1022996

Strand: Direct

Name: cspLA [H]

Synonym: Acid_0823

Alternate gene names: 116619951

Gene position: 1022793-1022996 (Clockwise)

Preceding gene: 116619950

Following gene: 116619952

Centisome position: 10.26

GC content: 59.31

Gene sequence:

>204_bases
GTGAAGGAAAAAGGCACCGTGAAATGGTTTAACGCCGCCAAGGGTTACGGATTTATCCAGCGATCGGGCGGCGATGACGT
GTTCGTTCACTTTTCCGCGATCCAGGCCAACGGCTACCGCTCGCTGGACGAAGGCGCGGAGGTGGAGTTCGAGGTCAAGC
AGGGGCCCAAAGGACTGCAGGCCGAGAACGTGGCACTGGTCTAG

Upstream 100 bases:

>100_bases
GTTTGCACGCGCCTCGCGAGTTCTTAGCAAGCCTCGAAAGTCCGCTTTCCTGGTTTGGGACACCCAAGAGCACGACGAAC
AATGATCAGGAGATTTGTAA

Downstream 100 bases:

>100_bases
GGTTACCTACAGGAATCAGCGCAGGGCTCTTCCGGGAGCCCTTCTTTCCTTCCCGCCCTCGCGCCGTGCAATAATAGGCA
CCAAATGTTGACGCGAGGCC

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: CspL [H]

Number of amino acids: Translated: 67; Mature: 67

Protein sequence:

>67_residues
MKEKGTVKWFNAAKGYGFIQRSGGDDVFVHFSAIQANGYRSLDEGAEVEFEVKQGPKGLQAENVALV

Sequences:

>Translated_67_residues
MKEKGTVKWFNAAKGYGFIQRSGGDDVFVHFSAIQANGYRSLDEGAEVEFEVKQGPKGLQAENVALV
>Mature_67_residues
MKEKGTVKWFNAAKGYGFIQRSGGDDVFVHFSAIQANGYRSLDEGAEVEFEVKQGPKGLQAENVALV

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=66, Percent_Identity=57.5757575757576, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=60.9375, Blast_Score=79, Evalue=8e-17,
Organism=Escherichia coli, GI1788126, Length=67, Percent_Identity=58.2089552238806, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=60, Blast_Score=76, Evalue=4e-16,
Organism=Escherichia coli, GI2367114, Length=67, Percent_Identity=53.7313432835821, Blast_Score=74, Evalue=1e-15,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=55.3846153846154, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=55.2238805970149, Blast_Score=72, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17533635, Length=70, Percent_Identity=50, Blast_Score=62, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24658520, Length=62, Percent_Identity=53.2258064516129, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7321; Mature: 7321

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKGTVKWFNAAKGYGFIQRSGGDDVFVHFSAIQANGYRSLDEGAEVEFEVKQGPKGLQ
CCCCCCEEEEECCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCC
AENVALV
CCCEEEC
>Mature Secondary Structure
MKEKGTVKWFNAAKGYGFIQRSGGDDVFVHFSAIQANGYRSLDEGAEVEFEVKQGPKGLQ
CCCCCCEEEEECCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCC
AENVALV
CCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003 [H]