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The map label for this gene is thiF [H]

Identifier: 116619877

GI number: 116619877

Start: 943423

End: 944454

Strand: Direct

Name: thiF [H]

Synonym: Acid_0749

Alternate gene names: 116619877

Gene position: 943423-944454 (Clockwise)

Preceding gene: 116619870

Following gene: 116619878

Centisome position: 9.47

GC content: 66.28

Gene sequence:

>1032_bases
TTGTTACCATCGCAGTTCATGGACCGGCGCGATCGCGACCGCTATTCGCGGCAAATTCTCTTCCCCGGAATCGGTGAGCG
CGGGCAGGAAGCGCTGCTGGCCGCGCACGTGGTGATTGCCGGCTGCGGCGCCCTGGGGAGCTTTCACGCGGCGGCGCTGG
CGCGCGCCGGAGTGGGACGCCTCACCCTGATCGATCGCGACTACATCGAGCCCAGCAACCTGCATCGCCAGTGGCTGTTC
GAAGAAGAGGATGCGGCCGAAGGTCTGCCGAAGGCGGCGGCGGCGGCTCGGAGGCTGGGGCGGATCAACTCGTCCATCCA
GGTGCATCCCGCGATCGCCGACCTCACCGCGGGGAATGCCGAAGAACTGCTGGGGGAAGCCGACGTCATTTTGGACGGCA
CCGACAATTTCGAGACGCGCTACCTGATCAACGATTTCGCGGTCAGCCGGGGGATTCCATGGGTGTACGGCGCCGCGGTC
GGGAGCTATGGATTGACCATGCCGGTGATTCCCGGTCAGAGCGCGTGCCTGCGCTGTGTCTACCCGGAGCCGCCCGGTAC
CGGCCAGCCCACGTGCGAAACGGCCGGAGTGCTGAACGTGATTGTCTCCGCGGTCGCCTCGATTCAGGTGGCGGATACGC
TCCGACTCCTGACCGGAGCGCCCCTGCGGGCGCGAATCACGACCTTCGACGTGTGGAAGGGCGGGATTCGCCAGATCGAC
GCGCCGGATCGCGATCCCGATTGCCCCACATGCGCTCGGCGTGAATTTCCTTGCCTTGTGGAGACGCAGCGTCAGCCTGC
CACCTTATGCGGGCGCAATGCCGTGCAGATTTCCGGCGTGGAGCGGCCGCTCGACCTGGCGGAGCTGAAGGCCCGGCTCG
AGCCGTTGGGCACGGTCCGCGCCAACGAGTACGCGCTGCGCTTTCAGACGGACGCGTACGAACTGACGGTGTTCGCCGAC
GGGCGGGCGATCGTAAAAGGAACCGGCGATACGGGTATCGCGCGCAGCCTCTACGCGCGGTATGTTGGATGA

Upstream 100 bases:

>100_bases
GAGCAGCGATCGCACCAAGTTCATGATAGCGTACGGTTTTCACAGCAATTGAGTGGCTGGACACACATTCATGAAATGGG
CCGGTGCCGGGGGGCCAGCT

Downstream 100 bases:

>100_bases
ATTATGGCGATTGCGTTAGTGACCGGAACCAGCACCGGAATTGGACAGGCGACGGCGGTGACGCTGGCGCGCGGAGGGCA
CACCGTGTACGCCACGATGC

Product: UBA/THIF-type NAD/FAD binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MLPSQFMDRRDRDRYSRQILFPGIGERGQEALLAAHVVIAGCGALGSFHAAALARAGVGRLTLIDRDYIEPSNLHRQWLF
EEEDAAEGLPKAAAAARRLGRINSSIQVHPAIADLTAGNAEELLGEADVILDGTDNFETRYLINDFAVSRGIPWVYGAAV
GSYGLTMPVIPGQSACLRCVYPEPPGTGQPTCETAGVLNVIVSAVASIQVADTLRLLTGAPLRARITTFDVWKGGIRQID
APDRDPDCPTCARREFPCLVETQRQPATLCGRNAVQISGVERPLDLAELKARLEPLGTVRANEYALRFQTDAYELTVFAD
GRAIVKGTGDTGIARSLYARYVG

Sequences:

>Translated_343_residues
MLPSQFMDRRDRDRYSRQILFPGIGERGQEALLAAHVVIAGCGALGSFHAAALARAGVGRLTLIDRDYIEPSNLHRQWLF
EEEDAAEGLPKAAAAARRLGRINSSIQVHPAIADLTAGNAEELLGEADVILDGTDNFETRYLINDFAVSRGIPWVYGAAV
GSYGLTMPVIPGQSACLRCVYPEPPGTGQPTCETAGVLNVIVSAVASIQVADTLRLLTGAPLRARITTFDVWKGGIRQID
APDRDPDCPTCARREFPCLVETQRQPATLCGRNAVQISGVERPLDLAELKARLEPLGTVRANEYALRFQTDAYELTVFAD
GRAIVKGTGDTGIARSLYARYVG
>Mature_343_residues
MLPSQFMDRRDRDRYSRQILFPGIGERGQEALLAAHVVIAGCGALGSFHAAALARAGVGRLTLIDRDYIEPSNLHRQWLF
EEEDAAEGLPKAAAAARRLGRINSSIQVHPAIADLTAGNAEELLGEADVILDGTDNFETRYLINDFAVSRGIPWVYGAAV
GSYGLTMPVIPGQSACLRCVYPEPPGTGQPTCETAGVLNVIVSAVASIQVADTLRLLTGAPLRARITTFDVWKGGIRQID
APDRDPDCPTCARREFPCLVETQRQPATLCGRNAVQISGVERPLDLAELKARLEPLGTVRANEYALRFQTDAYELTVFAD
GRAIVKGTGDTGIARSLYARYVG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=255, Percent_Identity=38.0392156862745, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1787048, Length=243, Percent_Identity=35.3909465020576, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87082356, Length=250, Percent_Identity=38, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17540406, Length=249, Percent_Identity=35.3413654618474, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321903, Length=249, Percent_Identity=31.7269076305221, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320598, Length=171, Percent_Identity=26.9005847953216, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24582879, Length=245, Percent_Identity=34.6938775510204, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28573937, Length=215, Percent_Identity=27.4418604651163, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 37048; Mature: 37048

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSQFMDRRDRDRYSRQILFPGIGERGQEALLAAHVVIAGCGALGSFHAAALARAGVGR
CCCHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCE
LTLIDRDYIEPSNLHRQWLFEEEDAAEGLPKAAAAARRLGRINSSIQVHPAIADLTAGNA
EEEEECCCCCCCCCHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHCCCCCCH
EELLGEADVILDGTDNFETRYLINDFAVSRGIPWVYGAAVGSYGLTMPVIPGQSACLRCV
HHHCCCCCEEEECCCCCCEEEEEHHHHHHCCCCEEEHHHHCCCCCEECCCCCCCCEEEEE
YPEPPGTGQPTCETAGVLNVIVSAVASIQVADTLRLLTGAPLRARITTFDVWKGGIRQID
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCEECC
APDRDPDCPTCARREFPCLVETQRQPATLCGRNAVQISGVERPLDLAELKARLEPLGTVR
CCCCCCCCCCHHCCCCCCEEECCCCCHHHHCCCCEEECCCCCCCCHHHHHHHHCCCCCEE
ANEYALRFQTDAYELTVFADGRAIVKGTGDTGIARSLYARYVG
CCCEEEEEECCEEEEEEEECCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLPSQFMDRRDRDRYSRQILFPGIGERGQEALLAAHVVIAGCGALGSFHAAALARAGVGR
CCCHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCE
LTLIDRDYIEPSNLHRQWLFEEEDAAEGLPKAAAAARRLGRINSSIQVHPAIADLTAGNA
EEEEECCCCCCCCCHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEEEEHHCCCCCCH
EELLGEADVILDGTDNFETRYLINDFAVSRGIPWVYGAAVGSYGLTMPVIPGQSACLRCV
HHHCCCCCEEEECCCCCCEEEEEHHHHHHCCCCEEEHHHHCCCCCEECCCCCCCCEEEEE
YPEPPGTGQPTCETAGVLNVIVSAVASIQVADTLRLLTGAPLRARITTFDVWKGGIRQID
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCEECC
APDRDPDCPTCARREFPCLVETQRQPATLCGRNAVQISGVERPLDLAELKARLEPLGTVR
CCCCCCCCCCHHCCCCCCEEECCCCCHHHHCCCCEEECCCCCCCCHHHHHHHHCCCCCEE
ANEYALRFQTDAYELTVFADGRAIVKGTGDTGIARSLYARYVG
CCCEEEEEECCEEEEEEEECCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]