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The map label for this gene is grpE [H]

Identifier: 116619861

GI number: 116619861

Start: 922090

End: 922581

Strand: Direct

Name: grpE [H]

Synonym: Acid_0733

Alternate gene names: 116619861

Gene position: 922090-922581 (Clockwise)

Preceding gene: 116619860

Following gene: 116619862

Centisome position: 9.25

GC content: 60.37

Gene sequence:

>492_bases
ATGGCGGAAGCTGAAGGCGGCGGGGAATCCCAGTCCGCCGGGCTTGCCGCCCAATGCGATCAACTTGCCGTCGAAAAGGC
GGAGCTTCAGGACCGTGTGCTGCGCGCGCGCGCCGAGTTTGATAACTTCCGCCGCCGCGCCGAACGTGAGCGCTCGGAAT
ACCTGCAGTTTGCCGGGATGGAGACGATCCGCGAGATCCTGCCCATCGTGGACGATTTCGAGCGCGCCCTCAAGGTGGAA
ACCGCCGACCGCGATTACGCCAAAGGCGTCGAGTTGATTTATCAGCGCATGTTGGATTCCCTGAAAAAGATGGGGTTGGA
GCCCATCGAAACCGCGGGGAAGAAATTCGATCCGAACCTGCACCAGGCCGTGGAACGCGTGCAGACCGAGGAGGCGGAAG
ACCAGTCCATCCTCGGCGAGTTCCAACGTGGATATAATTTCAAAGGAAAGCTCCTCAGGCCCGCGATGGTAAAAGTCGCG
GTCCACCCGTAA

Upstream 100 bases:

>100_bases
CGGGCGCTCGGGAGCGCACAGTTTTAGCGCCGCCGCGCGCGGCGCAGGAAAGCCAATTAAGGAAGTGAACACCCTGAACC
CTCAAGAAAATACTCCGGAA

Downstream 100 bases:

>100_bases
CGCCGTGAATCCTGTGTCAAAGAGAGATTATTACGAAGTACTCGGTGTCGGAAAACAAGCAGCCGATTCGGAACTAAAGG
CCGCTTACCGCGAACTCGCC

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MAEAEGGGESQSAGLAAQCDQLAVEKAELQDRVLRARAEFDNFRRRAERERSEYLQFAGMETIREILPIVDDFERALKVE
TADRDYAKGVELIYQRMLDSLKKMGLEPIETAGKKFDPNLHQAVERVQTEEAEDQSILGEFQRGYNFKGKLLRPAMVKVA
VHP

Sequences:

>Translated_163_residues
MAEAEGGGESQSAGLAAQCDQLAVEKAELQDRVLRARAEFDNFRRRAERERSEYLQFAGMETIREILPIVDDFERALKVE
TADRDYAKGVELIYQRMLDSLKKMGLEPIETAGKKFDPNLHQAVERVQTEEAEDQSILGEFQRGYNFKGKLLRPAMVKVA
VHP
>Mature_162_residues
AEAEGGGESQSAGLAAQCDQLAVEKAELQDRVLRARAEFDNFRRRAERERSEYLQFAGMETIREILPIVDDFERALKVET
ADRDYAKGVELIYQRMLDSLKKMGLEPIETAGKKFDPNLHQAVERVQTEEAEDQSILGEFQRGYNFKGKLLRPAMVKVAV
HP

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=144, Percent_Identity=38.1944444444444, Blast_Score=104, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17552458, Length=159, Percent_Identity=29.559748427673, Blast_Score=63, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324806, Length=160, Percent_Identity=26.25, Blast_Score=65, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24653432, Length=144, Percent_Identity=25.6944444444444, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 18516; Mature: 18385

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAEGGGESQSAGLAAQCDQLAVEKAELQDRVLRARAEFDNFRRRAERERSEYLQFAGM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETIREILPIVDDFERALKVETADRDYAKGVELIYQRMLDSLKKMGLEPIETAGKKFDPNL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHH
HQAVERVQTEEAEDQSILGEFQRGYNFKGKLLRPAMVKVAVHP
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
AEAEGGGESQSAGLAAQCDQLAVEKAELQDRVLRARAEFDNFRRRAERERSEYLQFAGM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETIREILPIVDDFERALKVETADRDYAKGVELIYQRMLDSLKKMGLEPIETAGKKFDPNL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHH
HQAVERVQTEEAEDQSILGEFQRGYNFKGKLLRPAMVKVAVHP
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA