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The map label for this gene is yfhA [H]

Identifier: 116619723

GI number: 116619723

Start: 724462

End: 726015

Strand: Direct

Name: yfhA [H]

Synonym: Acid_0588

Alternate gene names: 116619723

Gene position: 724462-726015 (Clockwise)

Preceding gene: 116619719

Following gene: 116619724

Centisome position: 7.27

GC content: 65.57

Gene sequence:

>1554_bases
ATGCTGTACATTAAATGTACGATGGCTTTCTTGACGCTGGCCCAGGATACGCTCCTCCGGGGCGTCTCACGGCTCGGCTA
CGTCAATCCCTTCCTGCCCGAGCGCATCGCCTGCGAACGCGCCGTTCTCGGTGACGAATTCCTGGAAGGCGAGCCCGTGT
GGAGCTACCGCGCCGAACATCCCGAGCCGCGTGCCAATGTCTGGCGCGCCATGGCCAAAATCCAGCCCCTAGTCGAACAG
CTGCGGACCCGCCTCGCTGCCGGAGCCTCCGCCCGCGAGCACGACCTGCAACTCTACGAGGACGCCGCGCTTCAGCTGAT
TTATCAGCGCTGCTACCCCAAGTTCTTCGCAGCCGGTTTCGGCGCCGAACAATCAAACTCCTCCCGCTGGCGCTTCTACA
ACGAGTTCCTCGCCGACTGGCGCCACTTCTTCCACATCGACGGTGTGCGATTCCCCTCGGAGCACGACCCGCGCCATACC
TTCGCCTGCTTCCGCCAGATCCAGCGCGCCTTCGACATCACCTTCCGCGACATCATCGGCAGTTCGCTCCCCGCCGCCCG
CCTCCGCGGCGCCATCTGGCAATCCATCTTCACCCATGACATGCGCCGCTACCGGCGCACTTTGTATGCACGCATGGGCG
AGTTCGCCACCCTCATCACCGGCCCTTCCGGCACCGGCAAGGAACTCGCCGCCCGCGCCATCGCCGAATCGCGCTACGTG
CCTTTCGACGATCGCCGCCTCGCGTTCGCCGACGATGCCGGCCAGGTATTCTTCCCCATCAACATCTCCGCCCTTTCGCC
CACGTTGGTGGAGTCCGAGCTCTTCGGACACCGCCGCGGCGCCTTTACGGGCGCGGTGGCCGACCGGCGCGGCTGGCTCG
AAACCTGTCCCGAGCTCGGCTCCGTCTTCCTCGACGAACTCGGTGACATGGACCCCGCCATCCAGGTCAAGTTGCTGCGT
GTCATCGAAACCCGCACCTTCCACCCAGTCGGCGATACCGCCGCGCGCCAGTTCCGTGGCAAGCTCATCGCCGCCACCAA
TCGCGACCTGCCCGACGCCATGCGAAAAGGACAGTTTCGCGAGGACCTCTACTACCGCCTCTGCTCCGACCAGATCTCGA
CCGCCTCCATGGCCGAGCAACTCGCCGCTTCCCCCGGCGTCCTGCGCGAGTTAGTGGTCTATATGTCGCGCCGCGTGGCC
GGCGGCGATGGCGATGCGCTCGCCGAAGAGGTCATGCCCTGGATCGAATCCAATCTTGGCCCCGATTACCCCTGGCCCGG
CAACTACCGCGAGCTCGAGCAGTGCGTGAAGAATCTGCTGATCCGCGGCAACTATCGCCCCGCCCGCGCGGCGTCCGAAG
ATCCCGCGGAGGAACTCGCAGCCCACGCGCGCGCCGGAACTCTTACCGCGGACGAGCTGCTCTCCCGCTACGTCACGCTC
GTCTACAGCCGCACCGGCAGCTACGAAGAGACGGCCCGCCGTCTCGCGCTCGACCGCCGCACCGTAAAAGCCAAGGTCGA
TTCAGCGCTGCTCGCCCGGCTGCACATTTCTTGA

Upstream 100 bases:

>100_bases
AATTGCACCCCGCCTGCCCAAACCTAACACACATATTGTGAGCAGTTTACGTTGCCAACCCACGTTTCCCCGGCCTAGCG
CTTATACATTAAATGTATGG

Downstream 100 bases:

>100_bases
AGTGCGAAACTGCACCTCGAGGGCCAGTTGGCGTACGATAATCCTTACGATGTCTTCGGCTCTTCGTTGGAGGGCGTTCG
CTTTCGCAGCGGTCGCCATT

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MLYIKCTMAFLTLAQDTLLRGVSRLGYVNPFLPERIACERAVLGDEFLEGEPVWSYRAEHPEPRANVWRAMAKIQPLVEQ
LRTRLAAGASAREHDLQLYEDAALQLIYQRCYPKFFAAGFGAEQSNSSRWRFYNEFLADWRHFFHIDGVRFPSEHDPRHT
FACFRQIQRAFDITFRDIIGSSLPAARLRGAIWQSIFTHDMRRYRRTLYARMGEFATLITGPSGTGKELAARAIAESRYV
PFDDRRLAFADDAGQVFFPINISALSPTLVESELFGHRRGAFTGAVADRRGWLETCPELGSVFLDELGDMDPAIQVKLLR
VIETRTFHPVGDTAARQFRGKLIAATNRDLPDAMRKGQFREDLYYRLCSDQISTASMAEQLAASPGVLRELVVYMSRRVA
GGDGDALAEEVMPWIESNLGPDYPWPGNYRELEQCVKNLLIRGNYRPARAASEDPAEELAAHARAGTLTADELLSRYVTL
VYSRTGSYEETARRLALDRRTVKAKVDSALLARLHIS

Sequences:

>Translated_517_residues
MLYIKCTMAFLTLAQDTLLRGVSRLGYVNPFLPERIACERAVLGDEFLEGEPVWSYRAEHPEPRANVWRAMAKIQPLVEQ
LRTRLAAGASAREHDLQLYEDAALQLIYQRCYPKFFAAGFGAEQSNSSRWRFYNEFLADWRHFFHIDGVRFPSEHDPRHT
FACFRQIQRAFDITFRDIIGSSLPAARLRGAIWQSIFTHDMRRYRRTLYARMGEFATLITGPSGTGKELAARAIAESRYV
PFDDRRLAFADDAGQVFFPINISALSPTLVESELFGHRRGAFTGAVADRRGWLETCPELGSVFLDELGDMDPAIQVKLLR
VIETRTFHPVGDTAARQFRGKLIAATNRDLPDAMRKGQFREDLYYRLCSDQISTASMAEQLAASPGVLRELVVYMSRRVA
GGDGDALAEEVMPWIESNLGPDYPWPGNYRELEQCVKNLLIRGNYRPARAASEDPAEELAAHARAGTLTADELLSRYVTL
VYSRTGSYEETARRLALDRRTVKAKVDSALLARLHIS
>Mature_517_residues
MLYIKCTMAFLTLAQDTLLRGVSRLGYVNPFLPERIACERAVLGDEFLEGEPVWSYRAEHPEPRANVWRAMAKIQPLVEQ
LRTRLAAGASAREHDLQLYEDAALQLIYQRCYPKFFAAGFGAEQSNSSRWRFYNEFLADWRHFFHIDGVRFPSEHDPRHT
FACFRQIQRAFDITFRDIIGSSLPAARLRGAIWQSIFTHDMRRYRRTLYARMGEFATLITGPSGTGKELAARAIAESRYV
PFDDRRLAFADDAGQVFFPINISALSPTLVESELFGHRRGAFTGAVADRRGWLETCPELGSVFLDELGDMDPAIQVKLLR
VIETRTFHPVGDTAARQFRGKLIAATNRDLPDAMRKGQFREDLYYRLCSDQISTASMAEQLAASPGVLRELVVYMSRRVA
GGDGDALAEEVMPWIESNLGPDYPWPGNYRELEQCVKNLLIRGNYRPARAASEDPAEELAAHARAGTLTADELLSRYVTL
VYSRTGSYEETARRLALDRRTVKAKVDSALLARLHIS

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=272, Percent_Identity=37.5, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789087, Length=231, Percent_Identity=39.8268398268398, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1790437, Length=302, Percent_Identity=34.4370860927152, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=31.0769230769231, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788550, Length=257, Percent_Identity=34.6303501945525, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=37.2294372294372, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790299, Length=227, Percent_Identity=35.6828193832599, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87082152, Length=228, Percent_Identity=35.5263157894737, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786524, Length=250, Percent_Identity=34.4, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=35.9307359307359, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787583, Length=300, Percent_Identity=30.6666666666667, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=33.6283185840708, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 58562; Mature: 58562

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYIKCTMAFLTLAQDTLLRGVSRLGYVNPFLPERIACERAVLGDEFLEGEPVWSYRAEH
CEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PEPRANVWRAMAKIQPLVEQLRTRLAAGASAREHDLQLYEDAALQLIYQRCYPKFFAAGF
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAEQSNSSRWRFYNEFLADWRHFFHIDGVRFPSEHDPRHTFACFRQIQRAFDITFRDIIG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHC
SSLPAARLRGAIWQSIFTHDMRRYRRTLYARMGEFATLITGPSGTGKELAARAIAESRYV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCC
PFDDRRLAFADDAGQVFFPINISALSPTLVESELFGHRRGAFTGAVADRRGWLETCPELG
CCCCCEEEEECCCCCEEEEEEHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHH
SVFLDELGDMDPAIQVKLLRVIETRTFHPVGDTAARQFRGKLIAATNRDLPDAMRKGQFR
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCCHHHHHCCCHH
EDLYYRLCSDQISTASMAEQLAASPGVLRELVVYMSRRVAGGDGDALAEEVMPWIESNLG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
PDYPWPGNYRELEQCVKNLLIRGNYRPARAASEDPAEELAAHARAGTLTADELLSRYVTL
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
VYSRTGSYEETARRLALDRRTVKAKVDSALLARLHIS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLYIKCTMAFLTLAQDTLLRGVSRLGYVNPFLPERIACERAVLGDEFLEGEPVWSYRAEH
CEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PEPRANVWRAMAKIQPLVEQLRTRLAAGASAREHDLQLYEDAALQLIYQRCYPKFFAAGF
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAEQSNSSRWRFYNEFLADWRHFFHIDGVRFPSEHDPRHTFACFRQIQRAFDITFRDIIG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHC
SSLPAARLRGAIWQSIFTHDMRRYRRTLYARMGEFATLITGPSGTGKELAARAIAESRYV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCC
PFDDRRLAFADDAGQVFFPINISALSPTLVESELFGHRRGAFTGAVADRRGWLETCPELG
CCCCCEEEEECCCCCEEEEEEHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHH
SVFLDELGDMDPAIQVKLLRVIETRTFHPVGDTAARQFRGKLIAATNRDLPDAMRKGQFR
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCCHHHHHCCCHH
EDLYYRLCSDQISTASMAEQLAASPGVLRELVVYMSRRVAGGDGDALAEEVMPWIESNLG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
PDYPWPGNYRELEQCVKNLLIRGNYRPARAASEDPAEELAAHARAGTLTADELLSRYVTL
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
VYSRTGSYEETARRLALDRRTVKAKVDSALLARLHIS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]