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The map label for this gene is lieA [H]

Identifier: 116619507

GI number: 116619507

Start: 465975

End: 467642

Strand: Direct

Name: lieA [H]

Synonym: Acid_0366

Alternate gene names: 116619507

Gene position: 465975-467642 (Clockwise)

Preceding gene: 116619506

Following gene: 116619508

Centisome position: 4.68

GC content: 63.61

Gene sequence:

>1668_bases
ATGAAAAAGCTGTTACTGGGCGCTGCCGTAGCTTCCCTGCTACTCGCGCAGGAGAAGGTCGATCAGGCTACCGATGCGCG
CATCCGTAGTGAGGAACTGGAGCATTCGCAGATCATGCACACGCTGCACATGCTCACCGACCGTTACGGGCCGCGTGTCA
CTGGTACCCCGAACCACGAAGCGGCCGCCAAGTGGGTGGTCAAACAACTCACCGAATGGGGTTTTAAGAACGCCCATCTG
GAGGCCTGGGAATTTGGGCACCCGGGCTGGAGCAACGAGCGCGCCACCGCCTTCCTGATTTCGCCGGTGCAGCAAAACTT
AAAATTCGAAGTGCTGGCGTGGACGCCCTCCACCAACGGGACCGTCACGGGGTCGGCGATCCAGATTGTGACGCCGCAGG
GTCCGTTGCCACCGGCGGCGGCCGATGCTCCGGCAACTGTGGGGCGCGGCCGCGGCGGGCGCGGCGGCGGTGCGGTGCGG
CTTGGCCCCACGAAAGCGGAAATGGAAGCCTGGGTCAAAGCTACCCAACCGGCGGTAAAGGGCAAGATCGTGCTGGTGGG
CAAGGCGGCGGTGATTCCGGTCAACTTCGATCCGCCGGCGAAGCGGCGTGCCGATGAAGATGTGAAGGCGCAGTACGATC
CCAACAACCCGAATGCGGGACGCGGGCGGGGCGGCTTCGGCGGCGGCGGACGCGGTGCGGCGACGCCCGATCCGGACCGG
CTGACGCCGGCACAGGTTTCCGAAGCGCTCGACAGCATGCTGCTCGCGGGCGGCGCGGTGGTGCGGTTGAACGACGCGGC
GCGCGGCGATGGAATCATCGTCGCCCAACAGCACCGCGCGTACGAGCCAAGCAAGACGGTGCCGACGCTGATTATCCGCA
ACGACGATTACGGCCGCATCGAACGCCTATTGGCCGATGGGGAAGACGTGAAGATGGAATTCAATATCGTCAATCACGTG
TATCCGGAAGGCAAGACGAGTTATAACGTGGTCGGCGAGATTCCAGGCACTGATAAAGCCGACGAGATCGTGATGCTCGG
CGGACATCTGGACTCCTGGCACGCGGCCACCGGCGCGACGGACAACGCGATCGGCTCTTCGATCATGATGGAAGCGGCGC
GGTTGATCCAGCAACTCGGGCTCAAGCCGCGGCGCACCATCCGGGTGGCGCTGTGGTCGGGTGAAGAGGAAGGTCTGCTT
GGGTCGCTGGCCTATGTGAAGCAGCACTTCGGCACCTTCGAAAATCCCAAGCCGGAATTTGCCAAACTGGATTGCTATTT
CAACGTGGATACCGGCACGGGACGGCTTCGCGGGGCTGGGATCTTCGGACCGGGGGAGGCCGCGGCGGTGCTGCGCCCTG
TGCTGGCGCAGTATACCGACTGGGGCGTGTTTGGTGCAAACTCCACGGGAAGCCGCGCGACCGGCGGCACGGACAGCACG
TCTTTCAACAACGCCGGATTGCCGGGCGTTGGTTTTCAGCAGGACCCGATCGAGTACAACTCGATGACGCACCACACCAA
CCTGGATACGTACGAGCGCATCATTCCGGACGATGTGCAGAAGGCGGCGGCGATCGTGGCCGCGTCCATCTGGCATGTCG
CCAATCGCGACCAGATGGTTCCGAGGTTCACCAGGGAGACGATGCCTGCGCCGGTTGTAGCGCGATAG

Upstream 100 bases:

>100_bases
CTGGAAGATAAGATCTACGAGATCGAAGACGAGCTCCGGCTGGCCGCGCAAGCGTAATCGCGCGATCTGCAGGCTCCAGG
TAAGCTATCCTGGATCGCAT

Downstream 100 bases:

>100_bases
AGGGCGGCCGCGTCGCAGCGAGGCGAGCCGGCTGAAAGCCGGCTGCAGGCAGGATTGCCTGCCCCACGGAGCAATTAGTG
GATATCACAGAGCTGGTAAC

Product: peptidase M28

Products: NA

Alternate protein names: LIE1 [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKKLLLGAAVASLLLAQEKVDQATDARIRSEELEHSQIMHTLHMLTDRYGPRVTGTPNHEAAAKWVVKQLTEWGFKNAHL
EAWEFGHPGWSNERATAFLISPVQQNLKFEVLAWTPSTNGTVTGSAIQIVTPQGPLPPAAADAPATVGRGRGGRGGGAVR
LGPTKAEMEAWVKATQPAVKGKIVLVGKAAVIPVNFDPPAKRRADEDVKAQYDPNNPNAGRGRGGFGGGGRGAATPDPDR
LTPAQVSEALDSMLLAGGAVVRLNDAARGDGIIVAQQHRAYEPSKTVPTLIIRNDDYGRIERLLADGEDVKMEFNIVNHV
YPEGKTSYNVVGEIPGTDKADEIVMLGGHLDSWHAATGATDNAIGSSIMMEAARLIQQLGLKPRRTIRVALWSGEEEGLL
GSLAYVKQHFGTFENPKPEFAKLDCYFNVDTGTGRLRGAGIFGPGEAAAVLRPVLAQYTDWGVFGANSTGSRATGGTDST
SFNNAGLPGVGFQQDPIEYNSMTHHTNLDTYERIIPDDVQKAAAIVAASIWHVANRDQMVPRFTRETMPAPVVAR

Sequences:

>Translated_555_residues
MKKLLLGAAVASLLLAQEKVDQATDARIRSEELEHSQIMHTLHMLTDRYGPRVTGTPNHEAAAKWVVKQLTEWGFKNAHL
EAWEFGHPGWSNERATAFLISPVQQNLKFEVLAWTPSTNGTVTGSAIQIVTPQGPLPPAAADAPATVGRGRGGRGGGAVR
LGPTKAEMEAWVKATQPAVKGKIVLVGKAAVIPVNFDPPAKRRADEDVKAQYDPNNPNAGRGRGGFGGGGRGAATPDPDR
LTPAQVSEALDSMLLAGGAVVRLNDAARGDGIIVAQQHRAYEPSKTVPTLIIRNDDYGRIERLLADGEDVKMEFNIVNHV
YPEGKTSYNVVGEIPGTDKADEIVMLGGHLDSWHAATGATDNAIGSSIMMEAARLIQQLGLKPRRTIRVALWSGEEEGLL
GSLAYVKQHFGTFENPKPEFAKLDCYFNVDTGTGRLRGAGIFGPGEAAAVLRPVLAQYTDWGVFGANSTGSRATGGTDST
SFNNAGLPGVGFQQDPIEYNSMTHHTNLDTYERIIPDDVQKAAAIVAASIWHVANRDQMVPRFTRETMPAPVVAR
>Mature_555_residues
MKKLLLGAAVASLLLAQEKVDQATDARIRSEELEHSQIMHTLHMLTDRYGPRVTGTPNHEAAAKWVVKQLTEWGFKNAHL
EAWEFGHPGWSNERATAFLISPVQQNLKFEVLAWTPSTNGTVTGSAIQIVTPQGPLPPAAADAPATVGRGRGGRGGGAVR
LGPTKAEMEAWVKATQPAVKGKIVLVGKAAVIPVNFDPPAKRRADEDVKAQYDPNNPNAGRGRGGFGGGGRGAATPDPDR
LTPAQVSEALDSMLLAGGAVVRLNDAARGDGIIVAQQHRAYEPSKTVPTLIIRNDDYGRIERLLADGEDVKMEFNIVNHV
YPEGKTSYNVVGEIPGTDKADEIVMLGGHLDSWHAATGATDNAIGSSIMMEAARLIQQLGLKPRRTIRVALWSGEEEGLL
GSLAYVKQHFGTFENPKPEFAKLDCYFNVDTGTGRLRGAGIFGPGEAAAVLRPVLAQYTDWGVFGANSTGSRATGGTDST
SFNNAGLPGVGFQQDPIEYNSMTHHTNLDTYERIIPDDVQKAAAIVAASIWHVANRDQMVPRFTRETMPAPVVAR

Specific function: A leucine-specific metalloprotease that plays a role in controlling the amount of leupeptin during colony development. Degrades leupeptin into three components, acetyl-leucine, leucine and argininal [H]

COG id: COG2234

COG function: function code R; Predicted aminopeptidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M28 family. M28A subfamily [H]

Homologues:

Organism=Homo sapiens, GI7706387, Length=256, Percent_Identity=30.859375, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI4885505, Length=96, Percent_Identity=44.7916666666667, Blast_Score=71, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25147314, Length=126, Percent_Identity=31.7460317460317, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71995331, Length=126, Percent_Identity=31.7460317460317, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71995324, Length=126, Percent_Identity=31.7460317460317, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322586, Length=215, Percent_Identity=27.906976744186, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR007484
- InterPro:   IPR002884 [H]

Pfam domain/function: PF01483 P_proprotein; PF04389 Peptidase_M28 [H]

EC number: NA

Molecular weight: Translated: 59488; Mature: 59488

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLLGAAVASLLLAQEKVDQATDARIRSEELEHSQIMHTLHMLTDRYGPRVTGTPNHE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
AAAKWVVKQLTEWGFKNAHLEAWEFGHPGWSNERATAFLISPVQQNLKFEVLAWTPSTNG
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEEECHHCCCEEEEEEECCCCCC
TVTGSAIQIVTPQGPLPPAAADAPATVGRGRGGRGGGAVRLGPTKAEMEAWVKATQPAVK
EEECCEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCC
GKIVLVGKAAVIPVNFDPPAKRRADEDVKAQYDPNNPNAGRGRGGFGGGGRGAATPDPDR
CEEEEEECEEEEEECCCCCHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
LTPAQVSEALDSMLLAGGAVVRLNDAARGDGIIVAQQHRAYEPSKTVPTLIIRNDDYGRI
CCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHH
ERLLADGEDVKMEFNIVNHVYPEGKTSYNVVGEIPGTDKADEIVMLGGHLDSWHAATGAT
HHHHCCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCC
DNAIGSSIMMEAARLIQQLGLKPRRTIRVALWSGEEEGLLGSLAYVKQHFGTFENPKPEF
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCC
AKLDCYFNVDTGTGRLRGAGIFGPGEAAAVLRPVLAQYTDWGVFGANSTGSRATGGTDST
EEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCC
SFNNAGLPGVGFQQDPIEYNSMTHHTNLDTYERIIPDDVQKAAAIVAASIWHVANRDQMV
CCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
PRFTRETMPAPVVAR
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MKKLLLGAAVASLLLAQEKVDQATDARIRSEELEHSQIMHTLHMLTDRYGPRVTGTPNHE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
AAAKWVVKQLTEWGFKNAHLEAWEFGHPGWSNERATAFLISPVQQNLKFEVLAWTPSTNG
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEEECHHCCCEEEEEEECCCCCC
TVTGSAIQIVTPQGPLPPAAADAPATVGRGRGGRGGGAVRLGPTKAEMEAWVKATQPAVK
EEECCEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCC
GKIVLVGKAAVIPVNFDPPAKRRADEDVKAQYDPNNPNAGRGRGGFGGGGRGAATPDPDR
CEEEEEECEEEEEECCCCCHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
LTPAQVSEALDSMLLAGGAVVRLNDAARGDGIIVAQQHRAYEPSKTVPTLIIRNDDYGRI
CCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHH
ERLLADGEDVKMEFNIVNHVYPEGKTSYNVVGEIPGTDKADEIVMLGGHLDSWHAATGAT
HHHHCCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCC
DNAIGSSIMMEAARLIQQLGLKPRRTIRVALWSGEEEGLLGSLAYVKQHFGTFENPKPEF
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCC
AKLDCYFNVDTGTGRLRGAGIFGPGEAAAVLRPVLAQYTDWGVFGANSTGSRATGGTDST
EEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCC
SFNNAGLPGVGFQQDPIEYNSMTHHTNLDTYERIIPDDVQKAAAIVAASIWHVANRDQMV
CCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
PRFTRETMPAPVVAR
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA