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The map label for this gene is rfbE [H]

Identifier: 116619427

GI number: 116619427

Start: 385352

End: 386425

Strand: Direct

Name: rfbE [H]

Synonym: Acid_0286

Alternate gene names: 116619427

Gene position: 385352-386425 (Clockwise)

Preceding gene: 116619426

Following gene: 116619434

Centisome position: 3.87

GC content: 63.31

Gene sequence:

>1074_bases
ATGCATGAAACCGTTCTGATCACAGGGGGCGCCGGTTTTGTCGGCTCCGCCCTGGCGATCGCGATCCGGCGCGCTCATCC
CGCCACCAAGGTCATCGCCTTCGATAACTTGCGGCGACGGGGTTCGGAAATGAACTTGCCGCGCCTCAAAGCGGAAGGTG
TGCGGTTTGTACACGGCGACGTGCGTTCGCTGGCGGACTTGACCGACATACGTCCCGCGCCGGACCTGATTCTGGAATGC
TCGGCGGAGCCTTCGGTGCTCGCGGGCTACGGAGGTTCGCCGGAATACCTGATCCACACCAATTTGAACGGGTGCTTCCA
TTGCCTGGAGATCGCCAGGCAGGCGAAGGCCGATTTTCTATTCGTCTCCACCAGCCGCGTGTATCCGGTGGCGCTGCTCA
ACGGACTGGGTTATGACGAGGGCGAGACGCGCTTTCACCTGAGGCCGGATCAGACCGTGACGGGCGCGAGCCAGTGCGGG
ATCAGCGAGATGTTTCCGCTGGACGGCGCACGCTCCTTGTATGGAATGACGAAGCTGGCAGCGGAGTTGATGGTGGCGGA
ATATGGCGACGCTTACGGGATGAGGTTTGTCATCGACCGCTGCGGCCTCTTGACCGGTCCGTGGCAGATGGCCAAGGCGG
ACCAGGGCGTGGTAGCGCTGTGGGTGGCGGCGCACTGCCTCAATCGGGGATTGAAGTATATCGGGTTCGGCGGGACCGGG
AAGCAGGTGCGCGACTTCCTGCACATCGACGACTTCTGCGACCTGGTGCTCGACCAGATTGCGAACTTCGACGCGTATGC
CGGGCACCGCTGGAACGTCGGCGGAGGCGTGAACAACAGCGTGTCCTTGCGCGAGGCTACGGAGCTTTGCCGGGAGATCA
CGGGCCGCACAGTAGAGGTGGTCTCGACCGATGAAAACCGGCCTTCCGACCTGCGTTCTTACATCACCGATCACCGCGCG
GTAACGGCCGTGCGTGGCTGGACGCCGCAGCGCGATGCGAGGCGCACGATCGCCGATATCGCGGGATGGATCGACGGCGA
AGGCGCGCAACTCCGCGACGTGTTGATGGGGTAG

Upstream 100 bases:

>100_bases
CTGTTCATCGTGCTGTACCTCTGGCTGGAAAAGCACCTGTCCCGCGGCGATTATCACCGCAAGGTGATTGCTCCGGCGGA
CGAGAGGACCACTCACATCA

Downstream 100 bases:

>100_bases
GAGGCCGGAAGGCGAAGCCGCCGGCCCGCATTACGCCTTGCGGCGGGCTTCTACGATTTCACCGATGATGCGCGGCAGAT
CGTATTCCAATTTCCACTTC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MHETVLITGGAGFVGSALAIAIRRAHPATKVIAFDNLRRRGSEMNLPRLKAEGVRFVHGDVRSLADLTDIRPAPDLILEC
SAEPSVLAGYGGSPEYLIHTNLNGCFHCLEIARQAKADFLFVSTSRVYPVALLNGLGYDEGETRFHLRPDQTVTGASQCG
ISEMFPLDGARSLYGMTKLAAELMVAEYGDAYGMRFVIDRCGLLTGPWQMAKADQGVVALWVAAHCLNRGLKYIGFGGTG
KQVRDFLHIDDFCDLVLDQIANFDAYAGHRWNVGGGVNNSVSLREATELCREITGRTVEVVSTDENRPSDLRSYITDHRA
VTAVRGWTPQRDARRTIADIAGWIDGEGAQLRDVLMG

Sequences:

>Translated_357_residues
MHETVLITGGAGFVGSALAIAIRRAHPATKVIAFDNLRRRGSEMNLPRLKAEGVRFVHGDVRSLADLTDIRPAPDLILEC
SAEPSVLAGYGGSPEYLIHTNLNGCFHCLEIARQAKADFLFVSTSRVYPVALLNGLGYDEGETRFHLRPDQTVTGASQCG
ISEMFPLDGARSLYGMTKLAAELMVAEYGDAYGMRFVIDRCGLLTGPWQMAKADQGVVALWVAAHCLNRGLKYIGFGGTG
KQVRDFLHIDDFCDLVLDQIANFDAYAGHRWNVGGGVNNSVSLREATELCREITGRTVEVVSTDENRPSDLRSYITDHRA
VTAVRGWTPQRDARRTIADIAGWIDGEGAQLRDVLMG
>Mature_357_residues
MHETVLITGGAGFVGSALAIAIRRAHPATKVIAFDNLRRRGSEMNLPRLKAEGVRFVHGDVRSLADLTDIRPAPDLILEC
SAEPSVLAGYGGSPEYLIHTNLNGCFHCLEIARQAKADFLFVSTSRVYPVALLNGLGYDEGETRFHLRPDQTVTGASQCG
ISEMFPLDGARSLYGMTKLAAELMVAEYGDAYGMRFVIDRCGLLTGPWQMAKADQGVVALWVAAHCLNRGLKYIGFGGTG
KQVRDFLHIDDFCDLVLDQIANFDAYAGHRWNVGGGVNNSVSLREATELCREITGRTVEVVSTDENRPSDLRSYITDHRA
VTAVRGWTPQRDARRTIADIAGWIDGEGAQLRDVLMG

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 38986; Mature: 38986

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHETVLITGGAGFVGSALAIAIRRAHPATKVIAFDNLRRRGSEMNLPRLKAEGVRFVHGD
CCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCHHCCEEEEHHH
VRSLADLTDIRPAPDLILECSAEPSVLAGYGGSPEYLIHTNLNGCFHCLEIARQAKADFL
HHHHHHHHHCCCCCCCEEECCCCCCEEECCCCCCCEEEEECCHHHHHHHHHHHHCCCCEE
FVSTSRVYPVALLNGLGYDEGETRFHLRPDQTVTGASQCGISEMFPLDGARSLYGMTKLA
EEECCCCCHHHHHHCCCCCCCCCEEEECCCCCCCCHHHCCCHHCCCCCCHHHHHHHHHHH
AELMVAEYGDAYGMRFVIDRCGLLTGPWQMAKADQGVVALWVAAHCLNRGLKYIGFGGTG
HHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCH
KQVRDFLHIDDFCDLVLDQIANFDAYAGHRWNVGGGVNNSVSLREATELCREITGRTVEV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCEEEE
VSTDENRPSDLRSYITDHRAVTAVRGWTPQRDARRTIADIAGWIDGEGAQLRDVLMG
EECCCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCC
>Mature Secondary Structure
MHETVLITGGAGFVGSALAIAIRRAHPATKVIAFDNLRRRGSEMNLPRLKAEGVRFVHGD
CCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCHHCCEEEEHHH
VRSLADLTDIRPAPDLILECSAEPSVLAGYGGSPEYLIHTNLNGCFHCLEIARQAKADFL
HHHHHHHHHCCCCCCCEEECCCCCCEEECCCCCCCEEEEECCHHHHHHHHHHHHCCCCEE
FVSTSRVYPVALLNGLGYDEGETRFHLRPDQTVTGASQCGISEMFPLDGARSLYGMTKLA
EEECCCCCHHHHHHCCCCCCCCCEEEECCCCCCCCHHHCCCHHCCCCCCHHHHHHHHHHH
AELMVAEYGDAYGMRFVIDRCGLLTGPWQMAKADQGVVALWVAAHCLNRGLKYIGFGGTG
HHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCH
KQVRDFLHIDDFCDLVLDQIANFDAYAGHRWNVGGGVNNSVSLREATELCREITGRTVEV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCEEEE
VSTDENRPSDLRSYITDHRAVTAVRGWTPQRDARRTIADIAGWIDGEGAQLRDVLMG
EECCCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]