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The map label for this gene is glnA [H]

Identifier: 116619212

GI number: 116619212

Start: 95861

End: 97270

Strand: Direct

Name: glnA [H]

Synonym: Acid_0067

Alternate gene names: 116619212

Gene position: 95861-97270 (Clockwise)

Preceding gene: 116619209

Following gene: 116619213

Centisome position: 0.96

GC content: 60.92

Gene sequence:

>1410_bases
ATGACTCCACAGGAAGTGCTGGATTACGCGAAAACGAATGGTGCGCAGCAACTGGATCTCCGGTTCACCGATATTCCGAG
CCTGCAGCATCATGTCTCTTATCCCATGAACCAGGTGGATGAGAGCAGCTTCGAAGAGGGCTTCGGCATGGACGGATCGA
GCATCCGCGGCTGGGCGGCGATCCACGAGAGCGACATGCTCCTCATCCCCGACTCCACCACCGCCTTCATGGATCCCTTC
GCCGAGGTACCCACCCTGGTGATGTACGGCGATGTGAAGGACCCCATCACCAAGCAGCGCTACGAGCGCGACCCGCGCTG
GATCGCTCAAAAGGCCGAGCTCTATTTGAAGAATAGCGGGGTCGCCGACACGTCCTATTTCGGCGCTGAGGCGGAGTTCT
TCATCTTCGATAACGTCAGCTTCGATCAGAACCAGCACTCCGGCTTCTACTACATCGATGCCGAAGAGGGCCGCTGGAAT
TCCGGCCGCCGCGAAAACAATCTGGGCTACCGCCCGCGCTACAAGGAAGGCTACTTCCCGGTGCCGCCCACGGACCACTA
CCAGGACCTGCGCAGCGAGATGGTCCGCACCATGATCGAGTGCGGCCTCAATATCGAGTGCCACCACCACGAAGTCGCCA
CCGGCGGACAGTGCGAGATCGATCAGAAGTATGACACGCTTGTGCGCAGCGCGGACAACATGATGCTGTACAAATATGTG
GTCCGCAACGTGGCCAACCAGTATGGCAAGACAGTCACCTTCATGCCCAAGCCCATCTTCGGCGATAACGGCAGCGGCAT
GCACTGCCACCAGAGCCTGTGGCGCGAGGGCAAGCCGCTCTTCGCCGGCGATAGCTACGCCGGGCTCAGCCAGATGGCGC
TCCACTACATCGGCGGACTGCTCAAGCACGCGCGCGCGCTTTCCGCCATCATTGCCCCCACCACCAACAGCTATAAGCGC
CTGGTGCCCGGTTACGAAGCGCCGGTCAACCTCGCCTATTCGCGCCGCAATCGCAGCGCCGCGGTGCGCATCCCGATGTA
CTCCGCGAGTCCCAAGACCAAGCGCGTGGAATTCCGTCCGCCGGACGCCTCCGCCAATCCGTACCTGGCCTTTGCCGCGA
TGCTGATGGCCGGGCTCGATGGCGTGATGAACCGCATCAATCCCGGCGAGCCGCTCGACAAAGACATCTACGACCTCTCG
CCCGAGGAAATGAAGAGCGTGCCCTCTATGCCAGGTTCGCTCGACGAAGCGCTCACCTGTCTGGAAGACGACCACGGTTT
CCTGATGCGCGGCGACGTTTTTACCGAAGAGCTGCTCGAGACCTTCATCGATTACAAACGCAAGAACGAAGCCGAGGCCG
TGCGTCTGCGCCCGCACCCCTACGAGTTCGCCCTCTACTACGACATCTGA

Upstream 100 bases:

>100_bases
CCACAACATAGAGCAGACCGGGCATGTATTGAGTATAGTGTCGCGAGTCGCCCCGGCGATCCAGTCGCTATACTCGAAAG
TTAGGACTTAGGAGAAATCG

Downstream 100 bases:

>100_bases
GCCGCGACCGTTAGGGAGCGATCTACACCTATGCACGGCACCATCGTTCAGGTAAGCATCTCGCCCGGTGGACTGCCCAA
GCGCGCGATCGCCGAAGGCA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTPQEVLDYAKTNGAQQLDLRFTDIPSLQHHVSYPMNQVDESSFEEGFGMDGSSIRGWAAIHESDMLLIPDSTTAFMDPF
AEVPTLVMYGDVKDPITKQRYERDPRWIAQKAELYLKNSGVADTSYFGAEAEFFIFDNVSFDQNQHSGFYYIDAEEGRWN
SGRRENNLGYRPRYKEGYFPVPPTDHYQDLRSEMVRTMIECGLNIECHHHEVATGGQCEIDQKYDTLVRSADNMMLYKYV
VRNVANQYGKTVTFMPKPIFGDNGSGMHCHQSLWREGKPLFAGDSYAGLSQMALHYIGGLLKHARALSAIIAPTTNSYKR
LVPGYEAPVNLAYSRRNRSAAVRIPMYSASPKTKRVEFRPPDASANPYLAFAAMLMAGLDGVMNRINPGEPLDKDIYDLS
PEEMKSVPSMPGSLDEALTCLEDDHGFLMRGDVFTEELLETFIDYKRKNEAEAVRLRPHPYEFALYYDI

Sequences:

>Translated_469_residues
MTPQEVLDYAKTNGAQQLDLRFTDIPSLQHHVSYPMNQVDESSFEEGFGMDGSSIRGWAAIHESDMLLIPDSTTAFMDPF
AEVPTLVMYGDVKDPITKQRYERDPRWIAQKAELYLKNSGVADTSYFGAEAEFFIFDNVSFDQNQHSGFYYIDAEEGRWN
SGRRENNLGYRPRYKEGYFPVPPTDHYQDLRSEMVRTMIECGLNIECHHHEVATGGQCEIDQKYDTLVRSADNMMLYKYV
VRNVANQYGKTVTFMPKPIFGDNGSGMHCHQSLWREGKPLFAGDSYAGLSQMALHYIGGLLKHARALSAIIAPTTNSYKR
LVPGYEAPVNLAYSRRNRSAAVRIPMYSASPKTKRVEFRPPDASANPYLAFAAMLMAGLDGVMNRINPGEPLDKDIYDLS
PEEMKSVPSMPGSLDEALTCLEDDHGFLMRGDVFTEELLETFIDYKRKNEAEAVRLRPHPYEFALYYDI
>Mature_468_residues
TPQEVLDYAKTNGAQQLDLRFTDIPSLQHHVSYPMNQVDESSFEEGFGMDGSSIRGWAAIHESDMLLIPDSTTAFMDPFA
EVPTLVMYGDVKDPITKQRYERDPRWIAQKAELYLKNSGVADTSYFGAEAEFFIFDNVSFDQNQHSGFYYIDAEEGRWNS
GRRENNLGYRPRYKEGYFPVPPTDHYQDLRSEMVRTMIECGLNIECHHHEVATGGQCEIDQKYDTLVRSADNMMLYKYVV
RNVANQYGKTVTFMPKPIFGDNGSGMHCHQSLWREGKPLFAGDSYAGLSQMALHYIGGLLKHARALSAIIAPTTNSYKRL
VPGYEAPVNLAYSRRNRSAAVRIPMYSASPKTKRVEFRPPDASANPYLAFAAMLMAGLDGVMNRINPGEPLDKDIYDLSP
EEMKSVPSMPGSLDEALTCLEDDHGFLMRGDVFTEELLETFIDYKRKNEAEAVRLRPHPYEFALYYDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=422, Percent_Identity=26.0663507109005, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1790301, Length=471, Percent_Identity=54.9893842887473, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI87081870, Length=185, Percent_Identity=31.8918918918919, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 53274; Mature: 53142

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQEVLDYAKTNGAQQLDLRFTDIPSLQHHVSYPMNQVDESSFEEGFGMDGSSIRGWAA
CCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCHHHCCHHHHHHCCCCCCCCCCCEEE
IHESDMLLIPDSTTAFMDPFAEVPTLVMYGDVKDPITKQRYERDPRWIAQKAELYLKNSG
ECCCCEEEECCCCHHHHCCHHHCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHEEEECCC
VADTSYFGAEAEFFIFDNVSFDQNQHSGFYYIDAEEGRWNSGRRENNLGYRPRYKEGYFP
CCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
VPPTDHYQDLRSEMVRTMIECGLNIECHHHEVATGGQCEIDQKYDTLVRSADNMMLYKYV
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEECHHHHHHHHCCCCHHHHHHH
VRNVANQYGKTVTFMPKPIFGDNGSGMHCHQSLWREGKPLFAGDSYAGLSQMALHYIGGL
HHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHH
LKHARALSAIIAPTTNSYKRLVPGYEAPVNLAYSRRNRSAAVRIPMYSASPKTKRVEFRP
HHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEECCCCCCEEEEEEEECCCCCCCEEEECC
PDASANPYLAFAAMLMAGLDGVMNRINPGEPLDKDIYDLSPEEMKSVPSMPGSLDEALTC
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHCCCCCCCHHHHHHH
LEDDHGFLMRGDVFTEELLETFIDYKRKNEAEAVRLRPHPYEFALYYDI
HCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEC
>Mature Secondary Structure 
TPQEVLDYAKTNGAQQLDLRFTDIPSLQHHVSYPMNQVDESSFEEGFGMDGSSIRGWAA
CHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCHHHCCHHHHHHCCCCCCCCCCCEEE
IHESDMLLIPDSTTAFMDPFAEVPTLVMYGDVKDPITKQRYERDPRWIAQKAELYLKNSG
ECCCCEEEECCCCHHHHCCHHHCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHEEEECCC
VADTSYFGAEAEFFIFDNVSFDQNQHSGFYYIDAEEGRWNSGRRENNLGYRPRYKEGYFP
CCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
VPPTDHYQDLRSEMVRTMIECGLNIECHHHEVATGGQCEIDQKYDTLVRSADNMMLYKYV
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEECHHHHHHHHCCCCHHHHHHH
VRNVANQYGKTVTFMPKPIFGDNGSGMHCHQSLWREGKPLFAGDSYAGLSQMALHYIGGL
HHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHH
LKHARALSAIIAPTTNSYKRLVPGYEAPVNLAYSRRNRSAAVRIPMYSASPKTKRVEFRP
HHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEECCCCCCEEEEEEEECCCCCCCEEEECC
PDASANPYLAFAAMLMAGLDGVMNRINPGEPLDKDIYDLSPEEMKSVPSMPGSLDEALTC
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHCCCCCCCHHHHHHH
LEDDHGFLMRGDVFTEELLETFIDYKRKNEAEAVRLRPHPYEFALYYDI
HCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]