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The map label for this gene is cca [H]

Identifier: 116517000

GI number: 116517000

Start: 1407472

End: 1408656

Strand: Reverse

Name: cca [H]

Synonym: SPD_1386

Alternate gene names: 116517000

Gene position: 1408656-1407472 (Counterclockwise)

Preceding gene: 116515624

Following gene: 116516141

Centisome position: 68.85

GC content: 44.05

Gene sequence:

>1185_bases
ATGCCTTCTGAATTTCAGAAGGCTTTACCAGTATTAGAAAAAATTAAAGAAGCAGGCTTTGAGGCTTATTTTGTTGGGGG
CTCTGTTCGAGATGCCCTTCTCCATAGCCCTATCCACGATGTGGATATTGCGACGTCTTCTTATCCAGAAGAGACCAAGC
AGATTTTTCCGCGAACAGCCGATATCGGAATCGAGCATGGAACCGTCTTGGTCTTAGATGGGGATGAGGAGTATGAGGTA
ACAACCTTTCGGACAGAGGATGTCTATGTGGACTATCGCAGACCCAGTGCGGTTTCCTTTGTACGATCGCTAGAAGAAGA
CCTCAAACGCCGTGATTTCACAGTCAACGCCTTTGCCTTGGATGAGACAGGAGAAATCGTTGACTTGTTCCATGGTTTAG
AAGATTTGGAAAAGCAAGTCTTGCGAGCAGTTGGAGTGGCTAGTGAGCGTTTCAACGAAGATGCTTTACGGATTATGCGT
GGTTTCCGTTTTCAGGCTAGTCTTGGTTTTGCACTTGAGCCAGAAACATTTAAAGCTATGAAGACCTTGACGCCGCTTTT
GGAGAAAATTTCTGTAGAGCGTACCTTCGTCGAGTTTGATAAACTCTTGCTGGCTCCATTTTGGAGAAGGGGCTTGGCTT
CCATGATTGAGAGTCAAGCTTATGACTATCTCCCTGATATGGCATCTAGCCAGGACAAGCTCAACAGACTGTTTGATTTG
GAGACTGATTTTACTTTTGAATCCTCTGAACAAGCCTGGGCGGCTTTACTATGGGCTTTGGAGATTGAAAATGCGCAGTC
ATTTTTGAAATCTTGGAAGACCTCACGCCAGTTTGCCAAGCAAGTTCAGGATTTGCTGATTATTTTGGCTCTGCGTGAAA
ATGGAGAATTGAGCAAGCGAGATTGTTATCGCTTTGACATAGATTTGCTTTTACAGGCTGAAAATCTTCGTCAAGCTCAA
GGAAAAGAAGTCAACCCACAAGCCATCACAGAAAAATACCAAAGCTTGACTATCCATGACAAGAAAGAGATTCAGATTAA
TGGCGGTATTTTGATCAAGGAATATGGCTATCAGCCAGGCCCAGACTTGGGAGAGATTTTAACAGAGATTGAGTTTGCCA
TTGTCGATGGAGAATTGGAGAATAATCGTGAAGCCATCCATGCTTACCTGAGGGAGAAAAAATGA

Upstream 100 bases:

>100_bases
GCATCTCCTTCATGACAGGAGTCAATTTGGGAATTAAAGAAGTTGTCAAGCGTCATGAGCTTGTCTATGGATTAGAACAC
TTATTATGAGATTAACGCAA

Downstream 100 bases:

>100_bases
GTGATTTTATCGTTGAAAAACTAAGTAAATCCGTTGGTGACAAGACCGTTTTTAGGGATATTTCCTTTATTATCCATGAC
TTAGACAGAATTGGTTTAAT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIATSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEV
TTFRTEDVYVDYRRPSAVSFVRSLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIESQAYDYLPDMASSQDKLNRLFDL
ETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQ
GKEVNPQAITEKYQSLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLREKK

Sequences:

>Translated_394_residues
MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIATSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEV
TTFRTEDVYVDYRRPSAVSFVRSLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIESQAYDYLPDMASSQDKLNRLFDL
ETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQ
GKEVNPQAITEKYQSLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLREKK
>Mature_393_residues
PSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIATSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVT
TFRTEDVYVDYRRPSAVSFVRSLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMRG
FRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIESQAYDYLPDMASSQDKLNRLFDLE
TDFTFESSEQAWAALLWALEIENAQSFLKSWKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQG
KEVNPQAITEKYQSLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLREKK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=194, Percent_Identity=37.1134020618557, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI87081691, Length=213, Percent_Identity=34.7417840375587, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789436, Length=189, Percent_Identity=37.5661375661376, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71995920, Length=218, Percent_Identity=34.8623853211009, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321016, Length=209, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281360112, Length=218, Percent_Identity=32.1100917431193, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357337, Length=218, Percent_Identity=32.1100917431193, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45158; Mature: 45026

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIATSSYPEETKQIFPRTA
CCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHH
DIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVRSLEEDLKRRDFTVNAFAL
CCCCCCCEEEEEECCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEE
DETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMRGFRFQASLGFALEPETFKAM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEECCCCEECCHHHHHH
KTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIESQAYDYLPDMASSQDKLNRLFDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
ETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSRQFAKQVQDLLIILALRENGELSKR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCC
DCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQSLTIHDKKEIQINGGILIKEYGYQPG
CCEEECHHHEEEHHHHHHHCCCCCCHHHHHHHHHHCEEECCCEEEEECCEEEEECCCCCC
PDLGEILTEIEFAIVDGELENNREAIHAYLREKK
CCHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
PSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIATSSYPEETKQIFPRTA
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHH
DIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVRSLEEDLKRRDFTVNAFAL
CCCCCCCEEEEEECCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEE
DETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMRGFRFQASLGFALEPETFKAM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEECCCCEECCHHHHHH
KTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIESQAYDYLPDMASSQDKLNRLFDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
ETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSRQFAKQVQDLLIILALRENGELSKR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCC
DCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQSLTIHDKKEIQINGGILIKEYGYQPG
CCEEECHHHEEEHHHHHHHCCCCCCHHHHHHHHHHCEEECCCEEEEECCEEEEECCCCCC
PDLGEILTEIEFAIVDGELENNREAIHAYLREKK
CCHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11442348 [H]