Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 116516710

GI number: 116516710

Start: 1313047

End: 1313577

Strand: Reverse

Name: ogt [H]

Synonym: SPD_1292

Alternate gene names: 116516710

Gene position: 1313577-1313047 (Counterclockwise)

Preceding gene: 116515345

Following gene: 116515586

Centisome position: 64.2

GC content: 42.56

Gene sequence:

>531_bases
ATGCAAAAGAAGTATGTAAAAATCCTCTACTCCTCACCAATTGGTATTCTATCACTTGTAGCTGATGACCATTATTTGTA
TGGAATTTGGGTTCAGGAGCAGAAGCATTTTGAGAGGGGACTAGGAGATGAAACGATAGAAGAAGTTGTTAGTCATCCTA
TTTTAGACCCAGTTATTGCTTGCTTAGATGATTACTTTAAAGGCAAGCCTCAGGATTTATCCAACTTGCTCTTGGCGCCA
ATCGGAACGAATTTTGAAAAGAGAGTTTGGGACTATTTACAGGGCATTCCTTATGGTCAGACAGTGACCTATGGACAAAT
TGCTCAAGACCTGCAAGTGGCTTCTGCTCAAGCAATTGGTGGAGCAGTGGGACGCAATCCTTGGTCTATCCTAGTACCTT
GTCATCGTGTGTTGGGAGCAGGCAAGCGTCTGACAGGTTATGCTGCAGGAGTGGAAAAGAAAGCTTGGCTCTTGGAGCAT
GAAGGAGTAGATTTTAAAGATAGAAACAATAGAAGGAGAAGCACATGTTAG

Upstream 100 bases:

>100_bases
ACAAGGTTATGCAACATATTTTTGAAAAACTTGGTTTTAAACAAGTTGGTAAGGTGCCAGTAGATGGCGAACGCTTGGCC
TATCAAAAATTAAAGAAATA

Downstream 100 bases:

>100_bases
AATTTATCGAATATCCCAAATGTTCAACTTGTAAAAAAGCAAAACAAGAATTAAATCAATTAGGTGTGGACTATAAAGCC
GTCCATATCGTGGAAGAAAC

Product: methylated-DNA--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDETIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAP
IGTNFEKRVWDYLQGIPYGQTVTYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
EGVDFKDRNNRRRSTC

Sequences:

>Translated_176_residues
MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDETIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAP
IGTNFEKRVWDYLQGIPYGQTVTYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
EGVDFKDRNNRRRSTC
>Mature_176_residues
MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDETIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAP
IGTNFEKRVWDYLQGIPYGQTVTYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
EGVDFKDRNNRRRSTC

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=49.0196078431373, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1788542, Length=82, Percent_Identity=40.2439024390244, Blast_Score=69, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115533068, Length=118, Percent_Identity=34.7457627118644, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=40, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=82, Percent_Identity=40.2439024390244, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19728; Mature: 19728

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDETIEEVVSHPILDPVIA
CCCCEEEEEECCCCHHHHHEECCCEEEEEEECCHHHHHHCCCHHHHHHHHHCCCHHHHHH
CLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTVTYGQIAQDLQVASAQAIG
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEHEGVDFKDRNNRRRSTC
HHCCCCCCEEHEEHHHHHCCCCCHHHHHCCCCHHHEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDETIEEVVSHPILDPVIA
CCCCEEEEEECCCCHHHHHEECCCEEEEEEECCHHHHHHCCCHHHHHHHHHCCCHHHHHH
CLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTVTYGQIAQDLQVASAQAIG
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEHEGVDFKDRNNRRRSTC
HHCCCCCCEEHEEHHHHHCCCCCHHHHHCCCCHHHEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]