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The map label for this gene is folC [H]

Identifier: 116516494

GI number: 116516494

Start: 185813

End: 187069

Strand: Direct

Name: folC [H]

Synonym: SPD_0183

Alternate gene names: 116516494

Gene position: 185813-187069 (Clockwise)

Preceding gene: 116516683

Following gene: 116515524

Centisome position: 9.08

GC content: 43.91

Gene sequence:

>1257_bases
ATGTGTATGTTTGAAGTAAAAGAATGGCTCCATAGTCGGATTGGTTTGAATTTTCGATCAGGTTTGGGCCGAATGCAGCA
AGCGGTGGATTTGTTAGGAAATCCTGAGCAGTCTTACCCTATTATCCACGTAACAGGGACTAATGGGAAAGGATCTACCA
TTGCTTTTATGAGGGAATTATTTATGGGGCATGGCAAAAAAGTTGCGACCTTTACCTCCCCTCATATCGTCTCTATCAAT
GACCGAATCTGCATTAATGGGCAACCTATAGCAGACGCAGACTTTATCCGTTTGGCTGATCAGGTCAAGGAGATGGAGAA
AACGCTTCTGCAAACTCCTGACCAGTTGTCCTTTTTTGAATTGCTGACCTTAGTTGCTTTTCTTTATTTTAGGGGGCAAG
AGGTGGATTTGGTTTTATTAGAAGTGGGAATTGGTGGCTTACTTGACACGACCAATGTGGTAACTGGAGAGCTTGCTGTC
ATCACCTCCATTGGGCTTGACCATCAAGAAACCTTGGGTGATAGTCTAGAAGCAATTGCAGAGCAGAAAGCTGGTATTTT
CAAGGCTGGTAAGAAGGCAGTGATTGCGAAATTGCCTCCAGAAGCTAGGCTTGTCTGTCAGAAAAAAGCCGAATCTTTAG
CTGTTAACCTTTATCAGGCAGGTCAAGATTTTTCAGTGCTGAATGGTGATTTTTCAAGCTCTTTACTAAATCTTTCACAG
CTGAACATAGGCTTAGAAGGAGTCTATCAGCAGGAGAATGCAGCCTTGGCGTTGCAAACTTTTCTTCTTTTTATGGGAGA
AGGAAAGGAAGCTGTTGATGAACAGGCTGTAAGAAAGGCCTTGGAACAGACCCATTGGGCTGGTCGCTTGGAGCGTATTC
GCCCACAGATTTACTTGGATGGTGCTCATAACCTCCCTGCCTTGACTCGCTTGGTTGAGTTTATCAAAGAAAAAGAGCAG
GAAGGCTATCGACCTCAAATCCTCTTTGGATCCTTGAAACGTAAGGATTATCAAGGGATGTTGGGTTATCTGACTGAAAA
TTTACCTCAAGTGGAACTCAAAATGACCGGCTTTGACTATCAAGGGTCTTTGGACGAGACAGATGTAACAGGTTACCATG
TAATTCCCTCTTACCGAGAATTTATCAGCAGTTTCGAAGAAAGGGCAGACGCTCAAGACTTGCTGTTCGTTACAGGGTCT
CTCTATTTTATCTCAGAAGTACGGGGCTACCTGCTGGACCGTGAGCAGATAAATTGA

Upstream 100 bases:

>100_bases
GATTAGCTAACCAGGTAAGAGGTCGGGACGAAAATCCTGGCCTTGTTTTTATTAGTAACCATACTTTAATGCGATAATTG
ATTGGACTTTTGTCAAGGAG

Downstream 100 bases:

>100_bases
CCCCCTTTTTTGAACTTGTTATACTAGGGGAATTGCTGGTTAGAGAAAATTTCTCTAAATTGGTAGCAGAAAGGAAATTC
ATCATGAAATTAAAAAGATT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MCMFEVKEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTGTNGKGSTIAFMRELFMGHGKKVATFTSPHIVSIN
DRICINGQPIADADFIRLADQVKEMEKTLLQTPDQLSFFELLTLVAFLYFRGQEVDLVLLEVGIGGLLDTTNVVTGELAV
ITSIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQAGQDFSVLNGDFSSSLLNLSQ
LNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVRKALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQ
EGYRPQILFGSLKRKDYQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDLLFVTGS
LYFISEVRGYLLDREQIN

Sequences:

>Translated_418_residues
MCMFEVKEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTGTNGKGSTIAFMRELFMGHGKKVATFTSPHIVSIN
DRICINGQPIADADFIRLADQVKEMEKTLLQTPDQLSFFELLTLVAFLYFRGQEVDLVLLEVGIGGLLDTTNVVTGELAV
ITSIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQAGQDFSVLNGDFSSSLLNLSQ
LNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVRKALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQ
EGYRPQILFGSLKRKDYQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDLLFVTGS
LYFISEVRGYLLDREQIN
>Mature_418_residues
MCMFEVKEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTGTNGKGSTIAFMRELFMGHGKKVATFTSPHIVSIN
DRICINGQPIADADFIRLADQVKEMEKTLLQTPDQLSFFELLTLVAFLYFRGQEVDLVLLEVGIGGLLDTTNVVTGELAV
ITSIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQAGQDFSVLNGDFSSSLLNLSQ
LNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVRKALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQ
EGYRPQILFGSLKRKDYQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDLLFVTGS
LYFISEVRGYLLDREQIN

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=342, Percent_Identity=33.6257309941521, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI66932990, Length=342, Percent_Identity=33.6257309941521, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1788654, Length=339, Percent_Identity=30.9734513274336, Blast_Score=138, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71984923, Length=427, Percent_Identity=26.6978922716628, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17553148, Length=427, Percent_Identity=26.6978922716628, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17553150, Length=427, Percent_Identity=26.6978922716628, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323760, Length=424, Percent_Identity=28.0660377358491, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324815, Length=380, Percent_Identity=27.8947368421053, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6322718, Length=176, Percent_Identity=25.5681818181818, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24641571, Length=311, Percent_Identity=32.7974276527331, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24581568, Length=327, Percent_Identity=25.0764525993884, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46682; Mature: 46682

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCMFEVKEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTGTNGKGSTIAFMREL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHH
FMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKEMEKTLLQTPDQLSFFE
HHCCCCEEEEECCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LLTLVAFLYFRGQEVDLVLLEVGIGGLLDTTNVVTGELAVITSIGLDHQETLGDSLEAIA
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHH
EQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQAGQDFSVLNGDFSSSLLNLSQ
HHHCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHH
LNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVRKALEQTHWAGRLERIRPQIYLD
HCCCHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
GAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKDYQGMLGYLTENLPQVELKMTGFDY
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHHHHHHHHHCCCCCEEEEEECCCC
QGSLDETDVTGYHVIPSYREFISSFEERADAQDLLFVTGSLYFISEVRGYLLDREQIN
CCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCHHCCC
>Mature Secondary Structure
MCMFEVKEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTGTNGKGSTIAFMREL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHH
FMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKEMEKTLLQTPDQLSFFE
HHCCCCEEEEECCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LLTLVAFLYFRGQEVDLVLLEVGIGGLLDTTNVVTGELAVITSIGLDHQETLGDSLEAIA
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHEEECCCCCHHHHHHHHHHHHH
EQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQAGQDFSVLNGDFSSSLLNLSQ
HHHCCHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHH
LNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVRKALEQTHWAGRLERIRPQIYLD
HCCCHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
GAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKDYQGMLGYLTENLPQVELKMTGFDY
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHHHHHHHHHCCCCCEEEEEECCCC
QGSLDETDVTGYHVIPSYREFISSFEERADAQDLLFVTGSLYFISEVRGYLLDREQIN
CCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]