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The map label for this gene is nagB

Identifier: 116516222

GI number: 116516222

Start: 1273866

End: 1274573

Strand: Reverse

Name: nagB

Synonym: SPD_1246

Alternate gene names: 116516222

Gene position: 1274573-1273866 (Counterclockwise)

Preceding gene: 116517173

Following gene: 116517095

Centisome position: 62.29

GC content: 41.81

Gene sequence:

>708_bases
ATGAAAGTTATTAAAGTTGAAAACCAAGTTCAAGGTGGAAAAGTTGCTTTTGAGATTTTGAAGGAAAAATTGGCTAATGG
AGCTCAAACTCTAGGACTTGCGACAGGAAGTAGCCCACTTGAATTTTACAAGGAAATTGTTGAGAGTAACCTTGATTTTT
CAAATCTAACCAGTGTCAACCTTGATGAGTATGTGGGCCTTGATGGGGACAATCCACAGTCTTATCGTTACTTCATGCAA
GAAAACTTGTTCAACCAAAAACCATTTAAAGAAAGTTTCTTGCCTCGTGGTGTTAAGGACAATGCTGAAGCTGAAGTTGA
ACGCTACAACCAAATTTTGGCTGACCATCCAGTTGACCTCCAAATCTTGGGAATCGGTCGCAATGGGCATATCGGCTTTA
ATGAGCCTGGTACTCCATTTGACAGTCAAACGCATCTAGTAGAACTTGACCAGTCTACTATCGAAGCCAATGCACGCTTC
TTTGCCAAGATTGAAGACGTCCCAACCCAAGCTATTTCAATGGGGATTAAAAACATTTTGGATGCCAAGTCTATTATTCT
TTTTGCTTACGGTGAGTCGAAAGCAGAAGCCATTGCCGGAACAGTATCTGGCCCAGTGACTGAGAACCTACCTGCAAGTA
GCCTCCAAAATCACCCTGATGTAACCATCATCGCAGATGCTGAAGCGCTTAGCTTACTCGAAAAATAA

Upstream 100 bases:

>100_bases
CAAGTTTGGACGCTCTTTTTTTAGAAATTAAATCGTTTTCACTTGACAAAAATTGGTCTATACCATATAATAAATATAGA
CAGAAATGGAGGATGAAAAG

Downstream 100 bases:

>100_bases
AAAAGCAATGTTCTATTGAACGCTTTCAACTCTTGTATAAATGGAAGAAAAAATCAAAATTAAACCGCATTTTTGCTTGA
CAATTATTCCTTTTACGTGT

Product: glucosamine-6-phosphate isomerase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYKEIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQ
ENLFNQKPFKESFLPRGVKDNAEAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNHPDVTIIADAEALSLLEK

Sequences:

>Translated_235_residues
MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYKEIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQ
ENLFNQKPFKESFLPRGVKDNAEAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNHPDVTIIADAEALSLLEK
>Mature_235_residues
MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYKEIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQ
ENLFNQKPFKESFLPRGVKDNAEAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNHPDVTIIADAEALSLLEK

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=243, Percent_Identity=33.7448559670782, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI19923881, Length=209, Percent_Identity=35.8851674641148, Blast_Score=130, Evalue=8e-31,
Organism=Escherichia coli, GI1786893, Length=240, Percent_Identity=32.0833333333333, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789530, Length=202, Percent_Identity=33.6633663366337, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI48994958, Length=237, Percent_Identity=29.1139240506329, Blast_Score=80, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17554876, Length=243, Percent_Identity=34.9794238683128, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24581960, Length=243, Percent_Identity=34.5679012345679, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI19920764, Length=243, Percent_Identity=34.5679012345679, Blast_Score=137, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_STRP2 (Q04JT5)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816713.1
- ProteinModelPortal:   Q04JT5
- SMR:   Q04JT5
- STRING:   Q04JT5
- EnsemblBacteria:   EBSTRT00000019765
- GeneID:   4441482
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1246
- eggNOG:   COG0363
- GeneTree:   EBGT00050000027970
- HOGENOM:   HBG725991
- OMA:   IIAEHPI
- ProtClustDB:   CLSK884089
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 25729; Mature: 25729

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 62-62 ACT_SITE 128-128 ACT_SITE 130-130 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYKEIVESNLDFSNLTSVN
CEEEEECCCCCCCHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHHCCCCHHHCCCCC
LDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNAEAEVERYNQILADHPVDL
HHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCE
QILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARFFAKIEDVPTQAISMGIKNIL
EEEEECCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCEEEEHHHCCHHHHHHHHHHHH
DAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNHPDVTIIADAEALSLLEK
CCCEEEEEEECCCCHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEECHHHHHHHCC
>Mature Secondary Structure
MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYKEIVESNLDFSNLTSVN
CEEEEECCCCCCCHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHHCCCCHHHCCCCC
LDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNAEAEVERYNQILADHPVDL
HHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCE
QILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARFFAKIEDVPTQAISMGIKNIL
EEEEECCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCEEEEHHHCCHHHHHHHHHHHH
DAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNHPDVTIIADAEALSLLEK
CCCEEEEEEECCCCHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA