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The map label for this gene is scrR [H]

Identifier: 116516061

GI number: 116516061

Start: 1557730

End: 1558695

Strand: Direct

Name: scrR [H]

Synonym: SPD_1535

Alternate gene names: 116516061

Gene position: 1557730-1558695 (Clockwise)

Preceding gene: 116516412

Following gene: 116516711

Centisome position: 76.13

GC content: 42.44

Gene sequence:

>966_bases
ATGGTCGCAAAACTAACTGATGTCGCCAAACTTGCAGGCGTCAGTCCTACTACCGTTTCTCGGGTTATCAATAAAAAAGG
ATATCTATCTGAGAAAACCATCCAAAAAGTCAATGAAGCCATGAGAGAATTAGGCTATAAACCCAACAACCTGGCTCGTA
GTCTGCAAGGAAAATCAGCTAAGTTAATCGGCTTGATTTTCCCCAATATTTCCAATGTTTTCTATGCAGAATTGATTGAT
AAATTGGAACACCAACTCTTCAAAAATGGTTACAAGACCATCATCTGCAACAGTGAACATGATTCTGAGAAGGAACGCGA
ATACATCGAAATGTTGGAAGCCAATCAGGTAGACGGCATCATTTCTGGTAGTCACAACCTAGGAATCGAAGACTACAATC
GTGTGACAGCGCCGATTATTTCCTTTGACCGAAACCTATCGCCAGACATCCCTGTTGTCTCCTCTGACAACTATGCTGGT
GGGGTTCTTGCTGCCCAAACCTTGGTCAAGACAGGTGCCCAGTCTATCATCATGATTACAGGGAATGACAATTCCAATTC
GCCAACCGGACTGCGCCACGCTGGTTTTGCATCCGTACTCCCAAAAGCTCCTATTATCAATGTTTCCAGTGACTTTTCTC
CCGTCAGAAAAGAAATGGAAATCAAGAATATCTTGACCCGGGAAAAACCAGATGCCATTTTTGCTTCGGATGATTTGACA
GCTATTCTGGTCATTAAAATCGCTCAAGAATTGGGCATTTCTGTCCCAAAAGAGCTCAAGGTCATCGGCTATGATGGGAC
CTACTTTATCGAAAATTACTACCCTCAATTGGCTACTATCAAGCAACCTTTGGAAGAGATTGCTTGTCTCACTATTGATC
TTCTCTTGCAAAAGATTGAAGGCAAGGAAGTCGCCACAACTGGTTACTTCTTACCAGTTACGCTATTACCAGGAAAAAGT
ATTTAA

Upstream 100 bases:

>100_bases
GGAGAAAAAGTATTTTCTGGTCGTGTCTTCCCACATGCGGACCAAAATGGTATCCTGATCAAATCTGGAAACCCAACTGG
AACTTACTATGAATTAGATT

Downstream 100 bases:

>100_bases
ACACAAGAAAACTCAGACCGATTCGTCTGAGTTTTTATGATCTTAAATTTTCGAGATAGCGCTGGGCTGTCTCTAGGTTA
AAGGTTTTATCTGAGATGAG

Product: sucrose operon repressor

Products: NA

Alternate protein names: Scr operon regulatory protein [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMRELGYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELID
KLEHQLFKNGYKTIICNSEHDSEKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKEMEIKNILTREKPDAIFASDDLT
AILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQLATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKS
I

Sequences:

>Translated_321_residues
MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMRELGYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELID
KLEHQLFKNGYKTIICNSEHDSEKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKEMEIKNILTREKPDAIFASDDLT
AILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQLATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKS
I
>Mature_321_residues
MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMRELGYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELID
KLEHQLFKNGYKTIICNSEHDSEKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKEMEIKNILTREKPDAIFASDDLT
AILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQLATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKS
I

Specific function: Negative regulator of scrB expression [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=33.0097087378641, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1787948, Length=313, Percent_Identity=31.3099041533546, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=29.5522388059701, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1790369, Length=324, Percent_Identity=26.5432098765432, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788474, Length=328, Percent_Identity=28.3536585365854, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786540, Length=328, Percent_Identity=29.2682926829268, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI48994940, Length=329, Percent_Identity=26.1398176291793, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1789202, Length=299, Percent_Identity=27.0903010033445, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786268, Length=211, Percent_Identity=35.5450236966825, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1787906, Length=344, Percent_Identity=25.8720930232558, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790715, Length=309, Percent_Identity=22.9773462783171, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787580, Length=326, Percent_Identity=24.5398773006135, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790689, Length=301, Percent_Identity=21.5946843853821, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35185; Mature: 35185

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMRELGYKPNNLARSLQGKSA
CCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCC
KLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDSEKEREYIEMLEANQVDGI
EEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCE
ISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAGGVLAAQTLVKTGAQSIIMIT
EECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEE
GNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKEMEIKNILTREKPDAIFASDDLT
CCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCH
AILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQLATIKQPLEEIACLTIDLLLQKIE
HHHHHHHHHHHCCCCCCCEEEEECCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
GKEVATTGYFLPVTLLPGKSI
CCEEEECCEEEEEEECCCCCC
>Mature Secondary Structure
MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMRELGYKPNNLARSLQGKSA
CCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCC
KLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDSEKEREYIEMLEANQVDGI
EEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCE
ISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAGGVLAAQTLVKTGAQSIIMIT
EECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEE
GNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKEMEIKNILTREKPDAIFASDDLT
CCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCH
AILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQLATIKQPLEEIACLTIDLLLQKIE
HHHHHHHHHHHCCCCCCCEEEEECCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
GKEVATTGYFLPVTLLPGKSI
CCEEEECCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9673256; 12397186 [H]