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The map label for this gene is queF

Identifier: 116515904

GI number: 116515904

Start: 1586556

End: 1586900

Strand: Direct

Name: queF

Synonym: SPD_1568

Alternate gene names: 116515904

Gene position: 1586556-1586900 (Clockwise)

Preceding gene: 116516780

Following gene: 116516731

Centisome position: 77.54

GC content: 45.8

Gene sequence:

>345_bases
ATGTCTCTGCTGGGACAAATCACTGCTCAGCCAGACTTTGCGACGATTCATATTTCCTACATTCCTGACAAGCTCTGTGT
CGAGTCAAAATCCCTCAAACTATATCTATTTAGCTACCGAAACCACGGAGATTTCCACGAAAACTGTATCAACACCATCG
GGAAAGACTTGGTCAACTTGCTAGACCCTCGCTATTTAGAAGTCTGGGGAAAATTCACTCCGCGCGGTGGCATTTCAATC
GACCCCTACTACAACTACGGTAAGCAAGGAACTAAGTATGAGGGCTTGGCAGAACAACGCCTCTTCCAACACGACCTTTA
TCCAGAGAAAATTGACAACCGCTAA

Upstream 100 bases:

>100_bases
CGAAGCTGTCACCTTTGCCTACGGCCAACATCATCACCTCGAAATTCAAATTACTAGAGAAATCGCTAAGGAACAGGGCA
TTCGTCACCATATCCTCGAT

Downstream 100 bases:

>100_bases
ACTCATACTCAATGAAAATCAAAGAGCAAACTAGGAAGTTAGCCGCAGGTTACTCAAAGCAGTGTTTTGAGGTTGTAGAT
GAAACTGACGAAGTCAGCTT

Product: GTP cyclohydrolase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 114; Mature: 113

Protein sequence:

>114_residues
MSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISI
DPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR

Sequences:

>Translated_114_residues
MSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISI
DPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR
>Mature_113_residues
SLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISID
PYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=77, Percent_Identity=37.6623376623377, Blast_Score=65, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_STRPN (Q97P67)

Other databases:

- EMBL:   AE005672
- PIR:   A95207
- RefSeq:   NP_346210.1
- EnsemblBacteria:   EBSTRT00000026656
- GeneID:   931058
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_1777
- TIGR:   SP_1777
- GeneTree:   EBGT00050000029526
- HOGENOM:   HBG294103
- OMA:   GIRHHIL
- ProtClustDB:   PRK13258
- BioCyc:   SPNE170187-1:SP_1777-MONOMER
- BRENDA:   1.7.1.13
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF06508 ExsB; PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 13246; Mature: 13115

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNL
CCCCCCCCCCCCEEEEEEEECCHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHH
LDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR
HCCHHEEHHCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
SLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNL
CCCCCCCCCCCEEEEEEEECCHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHH
LDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR
HCCHHEEHHCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916