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The map label for this gene is nadE

Identifier: 116515793

GI number: 116515793

Start: 1278942

End: 1279766

Strand: Reverse

Name: nadE

Synonym: SPD_1250

Alternate gene names: 116515793

Gene position: 1279766-1278942 (Counterclockwise)

Preceding gene: 116515881

Following gene: 116516411

Centisome position: 62.55

GC content: 47.27

Gene sequence:

>825_bases
ATGAGTTTGCAAGAAACGATTATCCAAGAGCTGGGTGTCAAACCAGTGATTGATGCCCAGGAAGAAATCCGTCGTTCTAT
TGATTTCTTAAAAAGATATCTGAAAAAACATCCCTTCCTAAAAACCTTTGTACTAGGGATTTCTGGGGGACAAGATTCAA
CCTTGGCAGGACGTTTGGCGCAATTAGCTATGGAAGAACTGCGAGCTGAAACGGGAGACGATAGCTACAAATTTATCGCT
GTCCGCCTGCCATACGGAGTGCAAGCTGATGAAGCAGATGCTCAAAAAGCCCTAGCCTTCATCCAGCCAGATGTCAGCTT
GGTTGTGAATATCAAGGAATCAGCTGATGCCATGACAGCTGCAGTTGAAGCGACAGGTAGTCCTGTTTCAGACTTCAACA
AGGGGAATATCAAGGCACGTTGCCGTATGATTGCTCAGTATGCCCTTGCTGGTTCCCATAGCGGAGCGGTCATTGGAACA
GACCACGCCGCGGAAAATATCACAGGTTTCTTTACCAAGTTTGGTGACGGCGGTGCGGATATTCTCCCTCTTTACCGCCT
CAATAAACGCCAAGGAAAACAACTCTTGCAGAAACTTGGCGCAGAGCCAGCCCTTTATGAAAAAATCCCAACGGCAGACC
TAGAAGAAGACAAACCAGGCCTAGCTGACGAAGTCGCACTTGGAGTCACCTATGCAGAGATTGACGATTACCTAGAAGGC
AAAACAATCAGCCCAGAAGCTCAAGCGACCATTGAAAACTGGTGGCACAAAGGCCAACACAAACGCCACTTACCCATCAC
CGTATTTGATGACTTTTGGGAGTAA

Upstream 100 bases:

>100_bases
CGCAGCACTATCCAGTGGATTTGGCGCGTGATGTATGGCAAGATAAGATGGACTTGATTGATAAGATGCGCAAGGAAGCC
CTTGGTGAAGGAGAAGAAGA

Downstream 100 bases:

>100_bases
AAAGGTCCGGGGGATCTTGAACCCCGAGTTTAGAAATAAGAAAATGAGGCAGATTCGGTAACTCGAAGAGTTCGATTTCA
TCGTCTTACCCCTGCAACGA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIA
VRLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEG
KTISPEAQATIENWWHKGQHKRHLPITVFDDFWE

Sequences:

>Translated_274_residues
MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIA
VRLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEG
KTISPEAQATIENWWHKGQHKRHLPITVFDDFWE
>Mature_273_residues
SLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAV
RLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGTD
HAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEGK
TISPEAQATIENWWHKGQHKRHLPITVFDDFWE

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=274, Percent_Identity=66.0583941605839, Blast_Score=373, Evalue=1e-105,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_STRP2 (Q04JT1)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816717.1
- ProteinModelPortal:   Q04JT1
- SMR:   Q04JT1
- STRING:   Q04JT1
- EnsemblBacteria:   EBSTRT00000019462
- GeneID:   4441558
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1250
- eggNOG:   COG0171
- GeneTree:   EBGT00050000027750
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- ProtClustDB:   PRK00768
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30135; Mature: 30004

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLA
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCHHHHHHHH
QLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTA
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
AVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGTDHAAENITGFFTKFGDGGAD
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCC
ILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEG
EEHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHCC
KTISPEAQATIENWWHKGQHKRHLPITVFDDFWE
CCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCC
>Mature Secondary Structure 
SLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLA
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCHHHHHHHH
QLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTA
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
AVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGTDHAAENITGFFTKFGDGGAD
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCC
ILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEG
EEHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHCC
KTISPEAQATIENWWHKGQHKRHLPITVFDDFWE
CCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA