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The map label for this gene is galR [H]

Identifier: 116515548

GI number: 116515548

Start: 1649464

End: 1650471

Strand: Direct

Name: galR [H]

Synonym: SPD_1635

Alternate gene names: 116515548

Gene position: 1649464-1650471 (Clockwise)

Preceding gene: 116516978

Following gene: 116516665

Centisome position: 80.61

GC content: 46.23

Gene sequence:

>1008_bases
ATGGCTACCTTAAAAGACATTGCACAGCTAGCCTCTGTCTCTATCGCGACCGTATCCCGTGTCCTCAACCGCGACCAGAG
CCTATCTGTTACAGAAGAAACCAGACACCGTATTTTAACCGTTGCTGAAGAGCTGGGCTACACCAAGCACCTCAAGACAG
GCGAGTCCCATAAACCCAAGCAAAAGATTGCCATTATTCAATGGGTCAGCGAACAAGGGGAGCTAGACGACCTCTACTAC
TACCAGATTCGCCTAGGAATAGAAAAAAGAGCCCAAGAGTTGGACTACGATATCTTGCGCTATTTTAATGACCACCCTTT
TACCCTAAGCGAGGAAGTGATTGGGATTCTCTGCATCGGAAAGTTTAGTCGAGCTCAGATTTCTGCCTTTGAAGAATACC
AAAAGCCTCTTGTATTTCTAGACAGCGATACACTTTCCCTGGGACATACCTGTATTATCACGGATTTTTACACTGCTATG
AAACAGGTTGTCGATTATTTCCTCAGTCAAGGGATGGACCGCATCGGGATTCTAACAGGGCTTGAGGAAACAACAGACCA
AGAAGAAATCATTCAGGACAAGCGTCTAGAAAACTTCAAAAACTACAGTCAAGCGAGGGGAATCTATCATGATGAACTGG
TCTTTCAAGGAAGATTTACTGCCCAGTCTGGCTATGACTTAATGAAGGAGGCCATTCAGAGCTTGGGAGACCAACTTCCG
CCAGCATTTTTCGCAGCCAGCGATAGTTTAGCTATCGGTGCCCTCCGTGCCCTCCAAGAAGCTGGAATCAGCCTGCCAGA
TCGCGTCAGCCTCATTTCATTCAACGACACCAGTCTGACCAAACAGGTCTATCCTCCCCTCTCTAGTATTACAGTTTATA
CTGAAGAAATGGGGCGAGCAGGTATGGATATTCTTAACAAAGAAGTACTCCACGGTCGCAAAATTCCTAGCCTGACCATG
CTGGGAACCAGACTGACATTAAGAGAAAGTACCCTAAATCAAGAATAG

Upstream 100 bases:

>100_bases
CCAAGGGATTTCCCAAGGTCAATCCTTTTTACTAAAATTTTACTAAACTATCAGTAAAAAGCAGAAAAATATGATATACT
AACCTAATCAAGGAGGATTT

Downstream 100 bases:

>100_bases
GATAACATAAAAAACGAATAGAGTTCTAAAACTCCTATTCGTTTTTTATTCGATTACAATCATAGACTTAATTGTCTTAC
GTTCATCCATATCTTTATAG

Product: galactose operon repressor

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEELGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYY
YQIRLGIEKRAQELDYDILRYFNDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGRFTAQSGYDLMKEAIQSLGDQLP
PAFFAASDSLAIGALRALQEAGISLPDRVSLISFNDTSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTM
LGTRLTLRESTLNQE

Sequences:

>Translated_335_residues
MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEELGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYY
YQIRLGIEKRAQELDYDILRYFNDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGRFTAQSGYDLMKEAIQSLGDQLP
PAFFAASDSLAIGALRALQEAGISLPDRVSLISFNDTSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTM
LGTRLTLRESTLNQE
>Mature_334_residues
ATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEELGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYY
QIRLGIEKRAQELDYDILRYFNDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAMK
QVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGRFTAQSGYDLMKEAIQSLGDQLPP
AFFAASDSLAIGALRALQEAGISLPDRVSLISFNDTSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTML
GTRLTLRESTLNQE

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=335, Percent_Identity=35.5223880597015, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1790369, Length=349, Percent_Identity=30.6590257879656, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787948, Length=350, Percent_Identity=26, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789068, Length=307, Percent_Identity=27.3615635179153, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790194, Length=324, Percent_Identity=25.3086419753086, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787580, Length=341, Percent_Identity=24.3401759530792, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789202, Length=306, Percent_Identity=25.8169934640523, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786540, Length=347, Percent_Identity=25.3602305475504, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788474, Length=312, Percent_Identity=23.3974358974359, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37880; Mature: 37748

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEELGYTKHLKTGESHKPK
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCH
QKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYFNDHPFTLSEEVIGILCIG
HHHHHHHHHCCCCCCCCEEEEEEEECHHHHHHHCCHHHHHHHCCCCCEEHHHHEEEEEEC
KFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAMKQVVDYFLSQGMDRIGILTG
CCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHC
LEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGRFTAQSGYDLMKEAIQSLGDQLP
CHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHCC
PAFFAASDSLAIGALRALQEAGISLPDRVSLISFNDTSLTKQVYPPLSSITVYTEEMGRA
CHHEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCCHHHHEEEHHHHCHH
GMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQE
HHHHHHHHHHCCCCCCCCEECCCEEEEEHHHCCCC
>Mature Secondary Structure 
ATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEELGYTKHLKTGESHKPK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCH
QKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYFNDHPFTLSEEVIGILCIG
HHHHHHHHHCCCCCCCCEEEEEEEECHHHHHHHCCHHHHHHHCCCCCEEHHHHEEEEEEC
KFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAMKQVVDYFLSQGMDRIGILTG
CCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHC
LEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGRFTAQSGYDLMKEAIQSLGDQLP
CHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHCC
PAFFAASDSLAIGALRALQEAGISLPDRVSLISFNDTSLTKQVYPPLSSITVYTEEMGRA
CHHEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCCHHHHEEEHHHHCHH
GMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQE
HHHHHHHHHHCCCCCCCCEECCCEEEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11157930 [H]