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The map label for this gene is argG

Identifier: 116496233

GI number: 116496233

Start: 2794535

End: 2795755

Strand: Direct

Name: argG

Synonym: LSEI_2811

Alternate gene names: 116496233

Gene position: 2794535-2795755 (Clockwise)

Preceding gene: 116496232

Following gene: 116496234

Centisome position: 96.52

GC content: 48.98

Gene sequence:

>1221_bases
ATGACAGAAAAAATCGTACTGGCATATTCAGGCGGCTTGGACACTTCGGTTGCCATCCCGTGGTTAAAAGACAAAGGCTA
TGAAGTGATTGCGGTGGTATTAAATGTCGGCCAGCCAAACGCCGACTTTGACGCCATTCAGCAAAAAGCACTGAACGTCG
GTGCACTCGATTCGATTGTGGTTGACGCCCAAGATGAATTTGCCGATCACTATGTTGCCCCGGTCATTAAAGCTAATGCC
TTGTACGAAGGCGACTATCCGCTGGTTTCCGCCTTATCTCGGCCATTGATCATTGAACATCTGGTGAAAATTGCCCACGC
GCAAAATGCCACAGCGATTGCCCATGGTTCAACCGGTAAAGGCAATGACCAAGTTCGCTTTGAAGCCGCAATCCACGCCT
TGGACCCGGAAATGAAAATCGAAGCCCCGATCCGTGATTTTCACTGGTCCCGTGAGGAAGAAATTGATTACGCCAAGGAG
CATAACGTCCCGGTACCAATTGGTAAAAAATCTCCTTATTCCATTGATGCCAACCTCTGGGGCCGTGCAAATGAAGCCGG
TATTCTTGAAAATCCTTGGAATCAGGCACCAGATGATGCTTGGGGTATGACAGTTTCCCCGGAGGCCGCTCCTGATGACC
CAACTTTTATTGATCTCACCTTCAAGCAAGGCGTTCCGGTGGCGTTAAATGATGAACCCATGGCACTGGCAACGATGATC
AAGGAGCTAAACAAGCTGGCTGGCGACAATGGCATTGGCCGGATTGATAAAATCGAAAATCGGCTAGTTGGCATCAAGTC
GCGTGAAGTCTATGAAGCACCAGCCGCTGCTGTCATTATGGCGGCCCACCACGATCTAGAAAACCTGACGCTTGAACGTG
ACGTTCAGCATTTTAAACCAACGATCGAAGATAAAATCACCAACATGATCTATGAAGCGCAGTGGATCTCACCGTTGTTT
GATGCCTTGATGGCGTTCATCGACAAGACCCAGGCTGTTGTTAATGGCACAGTGAAAATGAAGCTTTACAAAGGCAACGC
TACAGCCGTTGCTCGCAAATCCGCCCACAACTCGCTTTACGATGAAGATCTCGCGACTTACACAAGCGCTGATTCCTTCG
ATCAAGAAGCAGCAGCTGGCTTTATCAAACTCTGGACCTTGCCGACAACCGTTTTCGAGCAAGTCAACCATGTGCACTCC
GAGGAAAAACAACATGACTGA

Upstream 100 bases:

>100_bases
TTCACCCTTTCACGCCTGCTGCGACTGATTGAAAAACGCTTTAATTAAGATCAAAGTAACGCTCAAATAGCACCTCAAAA
CTAAGGAGGATTTTTAACTC

Downstream 100 bases:

>100_bases
TAAGTTATGGGGCGGTCGGTTTACTGAAAAAGCGGCACACTGGGTTGATGCTTTTGGAGCCTCCATCGGTTTTGATCAGC
AAATGGCCCAAGAAGATTTG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTEKIVLAYSGGLDTSVAIPWLKDKGYEVIAVVLNVGQPNADFDAIQQKALNVGALDSIVVDAQDEFADHYVAPVIKANA
LYEGDYPLVSALSRPLIIEHLVKIAHAQNATAIAHGSTGKGNDQVRFEAAIHALDPEMKIEAPIRDFHWSREEEIDYAKE
HNVPVPIGKKSPYSIDANLWGRANEAGILENPWNQAPDDAWGMTVSPEAAPDDPTFIDLTFKQGVPVALNDEPMALATMI
KELNKLAGDNGIGRIDKIENRLVGIKSREVYEAPAAAVIMAAHHDLENLTLERDVQHFKPTIEDKITNMIYEAQWISPLF
DALMAFIDKTQAVVNGTVKMKLYKGNATAVARKSAHNSLYDEDLATYTSADSFDQEAAAGFIKLWTLPTTVFEQVNHVHS
EEKQHD

Sequences:

>Translated_406_residues
MTEKIVLAYSGGLDTSVAIPWLKDKGYEVIAVVLNVGQPNADFDAIQQKALNVGALDSIVVDAQDEFADHYVAPVIKANA
LYEGDYPLVSALSRPLIIEHLVKIAHAQNATAIAHGSTGKGNDQVRFEAAIHALDPEMKIEAPIRDFHWSREEEIDYAKE
HNVPVPIGKKSPYSIDANLWGRANEAGILENPWNQAPDDAWGMTVSPEAAPDDPTFIDLTFKQGVPVALNDEPMALATMI
KELNKLAGDNGIGRIDKIENRLVGIKSREVYEAPAAAVIMAAHHDLENLTLERDVQHFKPTIEDKITNMIYEAQWISPLF
DALMAFIDKTQAVVNGTVKMKLYKGNATAVARKSAHNSLYDEDLATYTSADSFDQEAAAGFIKLWTLPTTVFEQVNHVHS
EEKQHD
>Mature_405_residues
TEKIVLAYSGGLDTSVAIPWLKDKGYEVIAVVLNVGQPNADFDAIQQKALNVGALDSIVVDAQDEFADHYVAPVIKANAL
YEGDYPLVSALSRPLIIEHLVKIAHAQNATAIAHGSTGKGNDQVRFEAAIHALDPEMKIEAPIRDFHWSREEEIDYAKEH
NVPVPIGKKSPYSIDANLWGRANEAGILENPWNQAPDDAWGMTVSPEAAPDDPTFIDLTFKQGVPVALNDEPMALATMIK
ELNKLAGDNGIGRIDKIENRLVGIKSREVYEAPAAAVIMAAHHDLENLTLERDVQHFKPTIEDKITNMIYEAQWISPLFD
ALMAFIDKTQAVVNGTVKMKLYKGNATAVARKSAHNSLYDEDLATYTSADSFDQEAAAGFIKLWTLPTTVFEQVNHVHSE
EKQHD

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=393, Percent_Identity=39.9491094147583, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI16950633, Length=393, Percent_Identity=39.9491094147583, Blast_Score=293, Evalue=3e-79,
Organism=Escherichia coli, GI1789563, Length=393, Percent_Identity=27.735368956743, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324514, Length=414, Percent_Identity=36.4734299516908, Blast_Score=280, Evalue=4e-76,
Organism=Drosophila melanogaster, GI21358151, Length=395, Percent_Identity=40.7594936708861, Blast_Score=296, Evalue=2e-80,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_LACC3 (Q033U7)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_807967.1
- ProteinModelPortal:   Q033U7
- SMR:   Q033U7
- STRING:   Q033U7
- GeneID:   4421381
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_2811
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- PhylomeDB:   Q033U7
- ProtClustDB:   PRK00509
- BioCyc:   LCAS321967:LSEI_2811-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44700; Mature: 44569

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2; PS00430 TONB_DEPENDENT_REC_1

Important sites: BINDING 86-86 BINDING 116-116 BINDING 118-118 BINDING 122-122 BINDING 122-122 BINDING 123-123 BINDING 126-126 BINDING 174-174 BINDING 259-259 BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKIVLAYSGGLDTSVAIPWLKDKGYEVIAVVLNVGQPNADFDAIQQKALNVGALDSIV
CCCEEEEEECCCCCCEEECCEECCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCHHEE
VDAQDEFADHYVAPVIKANALYEGDYPLVSALSRPLIIEHLVKIAHAQNATAIAHGSTGK
ECCCHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCC
GNDQVRFEAAIHALDPEMKIEAPIRDFHWSREEEIDYAKEHNVPVPIGKKSPYSIDANLW
CCCCEEEEEEHHHCCCCCEEECCHHHHCCCCHHHCCHHHHCCCCCCCCCCCCCEECCCCC
GRANEAGILENPWNQAPDDAWGMTVSPEAAPDDPTFIDLTFKQGVPVALNDEPMALATMI
CCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCEEECCCCHHHHHHH
KELNKLAGDNGIGRIDKIENRLVGIKSREVYEAPAAAVIMAAHHDLENLTLERDVQHFKP
HHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHCCHHEEEEEHHCCHHHHHHHHHHHHHCC
TIEDKITNMIYEAQWISPLFDALMAFIDKTQAVVNGTVKMKLYKGNATAVARKSAHNSLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEHHHHHCCHH
DEDLATYTSADSFDQEAAAGFIKLWTLPTTVFEQVNHVHSEEKQHD
HHHHHHHCCCCCCCHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEKIVLAYSGGLDTSVAIPWLKDKGYEVIAVVLNVGQPNADFDAIQQKALNVGALDSIV
CCEEEEEECCCCCCEEECCEECCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCHHEE
VDAQDEFADHYVAPVIKANALYEGDYPLVSALSRPLIIEHLVKIAHAQNATAIAHGSTGK
ECCCHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCC
GNDQVRFEAAIHALDPEMKIEAPIRDFHWSREEEIDYAKEHNVPVPIGKKSPYSIDANLW
CCCCEEEEEEHHHCCCCCEEECCHHHHCCCCHHHCCHHHHCCCCCCCCCCCCCEECCCCC
GRANEAGILENPWNQAPDDAWGMTVSPEAAPDDPTFIDLTFKQGVPVALNDEPMALATMI
CCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCEEECCCCHHHHHHH
KELNKLAGDNGIGRIDKIENRLVGIKSREVYEAPAAAVIMAAHHDLENLTLERDVQHFKP
HHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHCCHHEEEEEHHCCHHHHHHHHHHHHHCC
TIEDKITNMIYEAQWISPLFDALMAFIDKTQAVVNGTVKMKLYKGNATAVARKSAHNSLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEHHHHHCCHH
DEDLATYTSADSFDQEAAAGFIKLWTLPTTVFEQVNHVHSEEKQHD
HHHHHHHCCCCCCCHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA